Amount Of Chlorophyll-a Research Articles

The correlation between sea surface temperature and chlorophyll-a concentration

The examination of the correlation between sea surface temperature and chlorophyll-a concentration is essential for facilitating the surveillance and evaluation of marine ecosystems. Chlorophyll-a functions as a gauge of the prevalence of plant plankton and biomass in coastal waters, whereas sea surface temperature is a determinant that impacts the proliferation of plant plankton. This study aims to investigate the relationship between sea surface temperature and chlorophyll-a concentration on the surface of the sea. The case study was conducted in the upper Gulf of Thailand, Chonburi Province, in the year 2566 using data from the Aqua/MODIS satellite. Study results indicate that the amount of chlorophyll-a produced in 2022 ranged from 0.369 µg/L to 5.714 µg/L. When the amount of chlorophyll-a was correlated with the sea surface temperature, a high level of correlation was observed, with a regression coefficient of 0.859.

Seasonal biases in fluorescence-estimated chlorophyll-a derived from biogeochemical profiling floats

Marine phytoplankton biomass and chlorophyll-a concentration are often estimated from pigment fluorescence measurements, which have become routine despite known variability in the fluorescent response for a given amount of chlorophyll-a. Here, we present a near-global, monthly climatology of chlorophyll-a fluorescence measurements from profiling floats combined with ocean color satellite estimates of chlorophyll-a concentration to illuminate seasonal biases in the fluorescent response and expand upon previously observed regional patterns in this bias. Global biases span over an order of magnitude, and can vary seasonally by a factor of 10. An independent estimate of chlorophyll-a from light attenuation shows similar global patterns in the chlorophyll-fluorescence bias when compared to biases derived from satellite estimates. Without accounting for these biases, studies or models using fluorescence-estimated chlorophyll-a will inherit the seasonal and regional biases described here.

Cambio'nun Arthrospira Platensis Gomont ve Chlorella Vulgaris Beijerinck (Beijerinck) Üzerinde Oluşturduğu Oksidatif Stresin Ve Büyüme Değişimlerinin Değerlendirilmesi

This study aims to evaluate the toxicity effects of different concentrations of Cambio on Chlorella vulgaris (0-500 μg mL-1) and Arthrospira platensis (0-50 μg mL-1) algae by determining the changes in chlorophyll-a amount, OD 750 (biomass) and antioxidant parameters (the activities of Superoxide dismutase (SOD), Ascorbate peroxidase (APX), Glutathione reductase (GR) and the contents of malondialdehyde (MDA), hydrogen peroxide (H2O2), proline). A. platensis is being a cyanobacterium used commercially because of its high nutrient content. C. vulgaris used for medical and commercial purposes due to the capability of bioremediation, the structure of drug raw material, and nutrient compound. Ecotoxicological studies on these cosmopolitan algae are important for determining the harmful effects of chemicals on freshwater ecosystems. Cambio was toxic to A. platensis cells at the highest concentration, however, it stimulated the growth of C. vulgaris. For A. platensis application, the activity of Superoxide dismutase significantly decreased at moderate concentrations (p

Growth, Yield and Biochemical Qualities of Spinach (Spinacia oleracea) being Influenced by the Foliar Application of Chitosan

The study was carried out to evaluate the effects of various doses of foliar application of chitosan on spinach growth, yield, and quality characteristics (including chlorophyll-a, chlorophyll-b, total chlorophyll, carotenoids, total ash, and main nutrient contents). The Randomized Completely Block Design (RCBD) was used to set up the field experiment, with six treatment combinations [T0 = Control (no chitosan application), T1 = 100 ppm chitosan solution, T2 = 150 ppm chitosan solution, T3 = 200 ppm chitosan solution, T4 = 250 ppm chitosan solution, and T5 = 300 ppm chitosan solution] and three replications. The results revealed that applying chitosan at different doses significantly affected growth (viz., plant height, the number of leaves per plant) and yield of spinach. Moreover, due to the application of chitosan at various doses, there were notable differences observed in chlorophyll, total phenol, and ash contents of spinach. The spinach sample contained an average of 13.61–15.82 mg per g of chlorophyll–a, 9.16–10.18 mg per g of chlorophyll–b, and 22.77–25.85 mg per g of total chlorophyll. On the contrary, the effect of foliar application of chitosan at different doses showed a statistically significant difference in the P, K, Zn, and Fe contents of spinach. The highest amounts of chlorophyll-a, total ash, P, and K in spinach were obtained from treatment T3. However, the results showed that most of the biochemical properties of spinach were reduced by the higher doses (treatments T4 and T5) of foliar application of chitosan. Finally, according to the study's findings, foliar application of chitosan at the rate of 200 ppm significantly affects the growth, yield, pigments, total ash, P, and K contents of spinach.

Oxidative Damages of Two Neonicotinoid Pesticides to Arthrospira platensis (Gomont)

In this study, chlorophyll-a amount, OD 560 and antioxidant parameters (total SOD, APX, GR, MDA, H2O2 and Proline) were determined in order to understand the effects of Thiacloprid and Imidacloprid on Arthrospira platensis Gomont. Both Imidacloprid and Thiacloprid applications showed significant reductions in growth rate and chlorophyll-a content of A. platensis cultures with dose-dependent manner when the days and concentrations were compared each other. SOD activity significantly decreased in the Imidacloprid application while Thiacloprid caused a significant increase only at 75 µg mL-1 concentration. APX activity significantly increased in the Imidacloprid and Thiacloprid applications at 50 µg mL-1 and 35 µg mL-1 concentrations, respectively. Imidacloprid treatment increased GR activity at 20 and 30 µg mL-1 concentrations while GR activity increased at 15, 25 and 35 µg mL-1 Thiacloprid concentrations. The MDA content of A. platensis cultures did not change with Imidacloprid or Thiacloprid applications. The H2O2 content did not change at all different Imidacloprid concentrations. However, the H2O2 content decreased at 15 µg mL-1 and increased at 45 and 75 µg mL-1 Thiacloprid concentrations. Free proline content increased in the Imidacloprid and Thiacloprid applications at 100 µg mL-1 and 75 µg mL-1 concentrations, respectively. These neonicotinoid pesticides cause oxidative stress in A. platensis cells.

Comparative Toxicity of Heavy Metals Cd, Zn, and Pb to Three Acrocarpous Moss Species using Chlorophyll Contents

Mosses have often been used for biomonitoring because of their diversity of habitats, structural simplicity, and rapid multiplication rate. This research aimed to study the tolerance of heavy metal in 3 species of terricolous mosses i.e., Barbula consanguinea, Hyophila apiculata, and H. involuta. The gametophores of mosses were immersed at 4 concentrations of each of the heavy metals Cd, Pb, and Zn as well as in controls with no heavy metal. After 10, 20, and 30 days of exposure, mosses were extracted for chlorophyll-a in ethanol. The extracted chlorophyll-a was analyzed by spectrophotometer at 664 nm. The results revealed that the amount of chlorophyll-a in all species decreased with increasing concentrations of Cd, Pb, and Zn. The degree of metal toxicity for all species was Cd > Pb > Zn. By comparing the ratio of extracted chlorophyll-a in heavy metal-treated mosses to chlorophyll-a extracted in control mosses, the tolerance against 3 heavy metals in 3 mosses was H. involuta ≥ H. apiculata > B. consanguinea. Possibly, H. involuta can be used for biomonitoring of heavy metals in contaminated environments in the future. HIGHLIGHTS Mosses are often used for biomonitoring of heavy metal pollution because of their habitat diversity, structural simplicity, and rapid growth Three Thai mosses treated with Cd, Pb, and Zn showed a trend of a decrease in chlorophyll-a content with increasing concentrations of the metals The degree of metal toxicity for the mosses was Cd > Pb > Zn Among the three moss species, Hyophila involuta may be used for biomonitoring of heavy metals in contaminated environments in the future GRAPHICAL ABSTRACT

Identification of Photosynthetic Pigments extracted from Phaeodactylum tricornutum as High-Value Bioactive Compounds

The present study intends to provide information about a promising source of unique bioactive compounds and for choosing the favorable growth conditions of the diatom “Phaeodactylum tricornutum” suited to different applications. P. tricornutum was cultivated in the temperature range of 20-35°C, light intensity range of 275-538 μmolm-2s-1 and aeration rate range of 2.5- 3.5 Lmin-1. P. tricornutum extracts were analyzed in terms of photosynthetic pigment composition in comparison with a well-known green microalgae Chlorella miniata using the C18 column. Lutein, canthaxanthin, β-carotene and chlorophyll a were detected in C. miniata whereas in P. tricornutum extracts, chlorophyll a, zeaxanthin and β-carotene were identified. Quantitatively, P. tricornutum was distinguished to have greater amount of chlorophyll-a (113.66 mgmL-1) while C. miniata was predominated with a β-carotene content (14.20 mgmL-1). Considerable amounts of pigments were encountered in all of the P. tricornutum extracts obtained under the varying growth conditions. As the temperature was increased and the light intensity was decreased, the accumulation of chlorophyll a and β-carotene increased at the highest aeration rate. Conversely, at the highest light intensity of 538 μmolm-2s-1 and an aeration rate of 3.0 to 3.5 Lmin-1, the zeaxanthin content was maximized and limited at the temperature values over 27.5°C.

Interaction of TiO2 nanoparticles with the C. vulgaris: oxidative stress, lipid peroxidation and lipid amount.

Nanoparticles are widely used in many industries such as food and cosmetics. With increasing use, its spread to environmental environments is also increasing. Microalgae have an important place in the uptake of nanoparticles into the food chain. In this study, the effect of TiO2 nanoparticle on antioxidant enzyme activity, malondialdehyde, hydrogen peroxide, chlorophyll-a and total lipid amount in C. vulgaris microalgae has been investigated. As a result of the dose study, while the superoxide dismutase and ascorbate peroxidase enzyme activities decreased, the amount of MDA, H2O2 and chlorophyll-a increased. Depending on the times at different light:dark ratios, both an increase and a decrease occurred in the SOD, APX enzyme activity and the amount of MDA, H2O2. There was an increase in the amount of chlorophyll-a. In the time study, while the SOD and APX enzyme activities increased, the amount of MDA and H2O2 decreased. The amount of chlorophyll-a increased. In the total lipid study, the total lipid amount in the group with nano TiO2 increased compared to the control group. At the same time, C18:2 T (linoleic acid) has been found as fatty acid methyl ester in both groups.

Growth Effects of Corn Plants (Zea mays) on Agronomic Aspects of Oil Palm Tree Stands

The study aimed to determine the agronomic study of the tolerance of corn plants to conditions shaded by oil palm trees. This research was conducted from July to October 2022. At the Oil Palm Plantation of the Community of Bunut Seberang Village, Asahan Regency, North Sumatra. This study used a factorial randomized block design with two factors and three replications. The first treatment consisted of a shading level (P) with 4 treatment levels namely (P0) without shading, (P1) 35% shading, (P2) 70% shading. The second treatment consisted of a variety of corn (J) with 6 varieties, namely Exsotic Pertiwi (V1), Paragon (V2), Bonanza F1 (V3), Bisi 2 (V4) varieties. The best plant height parameter in the shade level treatment showed 35% shade (P1) resulted in the highest plant height of 113.98 cm, while in the Variety (V4) Bisi-2 variety treatment showed the highest plant, namely 112.83 when compared to other varieties. The effect of various varieties on leaf area showed a significant effect at the age of 6 and 8 WAP. The degree of shade and differences in varieties show the similarity in the quality of the green leaves. The level of shade showed a significant effect on chlorophyll-a and total chlorophyll-a and b where the highest amount of chlorophyll-a was found in the treatment without shade, which was not significantly different from the level of 70% shade, while varieties had a significant effect in producing net photosynthesis formed

Effect of Foliar Application of Chitosan on Growth, Yield and Nutritional Qualities of Red Amaranth (Amaranthus gangeticus L.)

Chitosan is a promising natural biopolymer that stimulates growth, increases yield, and boosts-up plants' immune systems. A field experiment was conducted to study the effect of foliar application of chitosan on growth, yield and quality attributes (viz. chlorophyll-a, chlorophyll-b, total chlorophyll, carotenoids, total ash and major nutrient contents) of red amaranth. The field experiment was laid out in a Randomized Completely Block Design (RCBD) with three replications and six treatments [viz. T0 = Control (no chitosan application); T1 = 100 mg L-1; T2 = 150 mg L-1; T3 = 200 mg L-1; T4 = 250 mg L-1 and T5 = 300 mg L-1 chitosan solution]. The study results revealed that the application of chitosan at different doses had no significant effect on plant height, the number of leaves plant-1, and the moisture content of red amaranth. But there were significant variations in stem diameter, root length, and yield of red amaranth. Similarly, the application of chitosan at different doses also showed a significant effect on chlorophyll contents, carotenoids and total ash contents of red amaranth. The average chlorophyll-a, chlorophyll-b, total chlorophylls, carotenoids and ash content of red amaranth ranged from 6.02-9.51, 3.67-5.92 and 9.68-15.15 mg g-1 tissue, 0.20-0.28 µg g-1 sample (fresh wt.), and 16.36-17.69%, respectively. Among the major nutrient elements, the amount of Ca, Mg, Na, K and P in red amaranth was statistically insignificant among the treatments. On the contrary, the effect of foliar application of chitosan at different doses disclosed a statistically significant difference in S, Zn and Fe contents of red amaranth. The highest amounts of chlorophyll-a, carotenoids, total ash, S, and Fe in red amaranth were obtained from treatment T5. Finally, the study results concluded that foliar application of chitosan at 300 mg L-1 has a significant positive effect on the growth, yield, pigments, S, and Fe contents of red amaranth.

Aprotic and protic solvent for extraction of chlorophyll from various plants: Chemical characteristic and analysis

The extraction efficiencies of acetone (80%), ethanol, DMSO, and DMF for chlorophyll extraction from mango, pine, spinach, and hibiscus have been compared. Acetone (80%) has yielded highest amount of chlorophyll-a (chl-a) from pine and ethanol has yielded highest amount of chlorophyll-b (chl-b) from mango leaves. The chl-a/b ratio ranges between 2.481 - 3.794, showing insignificant effect of solvent polarity. The UV - Vis absorption spectra has shown changes with solvent polarity with the polar protic solvent having a broad peak around 664 nm. There has been an insignificant effect of the solvent polarity on extraction of chlorophylls from the selected plants. The ANOVA results have shown a statistically significant difference in chlorophyll extraction efficiencies of different solvents. The absorbance values in the spectral region between 500 - 550 nm has been found to be less than 10 % of the absorbance at 664 nm, indicating tracer amounts of pheophytins and chlorophyll by-products in the solution.

New Approach for Analyzing Marine Ecosystem Structure Using Bayesian Networks

Aquatic ecosystems of the Black Sea are complex multiparametric systems with a hierarchical structure. Thus, the main goal of our research was to investigate possibilities of using Bayesian networks to study the structure fo the natural systems in the Black Sea. We used CMEMS Black Sea environmental dataset, which consists of 7 different variables, that, in our opinion, can describe structural relations in the Black Sea ecosystem -- sea surface temperature and salinity, concentrations of nitrates and phosphates, amount of chlorophyll-a and net primary production and also dissolved oxygen concentration. We think, that these variables can generally define interactions in water environment of the Black Sea, organisms, that live there and human activity. As a modelling result, we receive a structure of environmental variables interactions. At the top of this structure is a dissolved oxygen, as a final result of the ecosystem functioning. Further, we think it's more appropriate to use Dynamic Bayesian networks for investigation of spatio-temporal changes to distinguish main drivers of changes and provide more balanced management of natural territories.

Macrophyte Diversity as a Response to Extreme Conditions in the Post-Mining Lakes of the Muskau Arch (West Poland)

The pit lakes localized in the eastern part of the Muskau Arch (Western Poland) developed as a result of lignite mining at the turn of the 20th century. The formation of the lakes varied over time and was determined by many hydrogeochemical and biological factors, which resulted in very diverse ecosystems. Thirty of these lakes were studied in the vegetation seasons of 2016 and 2017. The aim of the study was to identify the main factors influencing the diversity of macrophyte vegetation growing under very extreme and diversified habitat conditions. Therefore, in each lake the numbers of macrophyte species and communities were determined, and the water was characterized by 26 physico-chemical parameters and Chl a. Additionally, the genesis, age, area and maximum depth of each lake was determined. The results showed high diversity of habitat conditions. Based on the physical and chemical water properties, four different clusters of lakes were distinguished. The multiple statistical comparisons showed that the main factors responsible for lake differentiation were pH, water mineralization and to a lesser extent, nutrient concentration and water transparency. The physico-chemical differentiation was reflected in the significant differences in the number of species, number of communities, maximum vegetation extent and amount of chlorophyll-a per lake. The correlational analyses confirmed significant relationships between habitat conditions and macrophyte flora diversity in the lakes. Some macrophyte species were found in very extreme environments not previously reported in the literature. Our results showed that in addition to natural processes affecting species diversity in the lakes, the negative impact of anthropogenic pressures (neutralization and fertilization of the water), which leads to the degradation of their unique character, is also an important factor.

Adaptation and Validation of a Sentinel-Based Chlorophyll-a Retrieval Software for the Central European Freshwater Lake, Balaton

The importance of lakes and reservoirs leads to the high need for monitoring lake water quality both at local and global scales. Remote sensing is a rapidly evolving, versatile technology that can be successfully applied in several economic and scientific fields. Numerous studies demonstrate the applicability of satellites in algae detection and monitoring. Algae play an essential role in aquatic ecosystems, although their overgrowth poses a serious risk. Overgrowth of algae, also known as algal bloom, has serious ecological, social, economic and health effects. The research area of our study was Lake Balaton, the largest lake in Central Europe. The aim was to find the most appropriate algorithm(s) for the inland lake to define the chlorophyll-a amount. In addition, two new algorithms were developed based on the reflectance values of the satellite image. The results show that the two highest correlations were performed by the newly validated, blue/green ratio-based algorithm, Chl-aB/G (r = 0.93) and the chl_re_oc2 algorithm (r = 0.86) of the Acolite software. Although the Acolite software was not developed for lakes but for marine waters, it is also applicable to inland waters.

Identification The Content of Lead (Pb), Mercury (Hg), Chlorophyll-a, and Cell Morphology of Seaweed Kappaphycus alvarezii in Bluto and Saronggi Water, Sumenep, Madura, East Java

Kappaphycus alvarezii is one of Indonesia's export commodities because it has high economic value as food and industry. One area in East Java which is the center of seaweed cultivation is Sumenep regency such as Saronggi and Bluto. Currently Sumenep Regency is an area for oil and gas exploration and exploitation. Types of pollutants resulting from these activities cause pollution in aquatic environments such as lead (Pb), mercury (Hg) and cadmium (Cd). The heavy metals found in the waters can be absorbed and accumulated in the seaweed thallus. The purpose of this research is to know the heavy metal content of Pb, Hg, Cd, chlorophyll-a, and cell morphology in E. cottonii in Bluto and Saronggi waters. This research is survey and descriptive research. The results showed that there are differences in heavy metal content of lead in E. cottonii, seawater, and sediments in Bluto waters and Saronggi waters. The waters of Bluto have a lower amount of chlorophyll-a than the Saronggi Waters. Cell wall thickness in E. cottonii in Saronggi Waters has thinner cell walls than Bluto waters. Measurement of water quality in Bluto and Saronggi waters through temperature, pH, salinity, brightness and DO parameters. The measurement results from both waters are not any striking difference and under optimal conditions.

Toxicity, Bioaccumulation and Biotransformation of Glucose-Capped Silver Nanoparticles in Green Microalgae Chlorella vulgaris.

Silver nanoparticles (AgNPs) are one of the most widely used nanomaterials in consumer products. When discharged into the aquatic environment AgNPs can cause toxicity to aquatic biota, through mechanisms that are still under debate, thus rendering the nanoparticles (NPs) effects evaluation a necessary step. Different aquatic organism models, i.e., microalgae, mussels, Daphnia magna, sea urchins and Danio rerio, etc. have been largely exploited for NPs toxicity assessment. On the other hand, alternative biological microorganisms abundantly present in nature, i.e., microalgae, are nowadays exploited as a potential sink for removal of toxic substances from the environment. Indeed, the green microalgae Chlorella vulgaris is one of the most used microorganisms for waste treatment. With the aim to verify the possible involvement of C. vulgaris not only as a model microorganism of NPs toxicity but also for the protection toward NPs pollution, we used these microalgae to measure the AgNPs biotoxicity and bioaccumulation. In particular, to exclude any toxicity derived by Ag+ ions release, green chemistry-synthesised and glucose-coated AgNPs (AgNPs-G) were used. C. vulgaris actively internalised AgNPs-G whose amount increases in a time- and dose-dependent manner. The internalised NPs, found inside large vacuoles, were not released back into the medium, even after 1 week, and did not undergo biotransformation since AgNPs-G maintained their crystalline nature. Biotoxicity of AgNPs-G causes an exposure time and AgNPs-G dose-dependent growth reduction and a decrease in chlorophyll-a amount. These results confirm C. vulgaris as a bioaccumulating microalgae for possible use in environmental protection.

Effect of iron and magnesium addition on population dynamics and high value product of microalgae grown in anaerobic liquid digestate

In this study, FeSO4 supplementation ranging from 0 to 4.5 mM, and MgSO4 supplementation ranging from 0 to 5.1 mM were investigated to observe the effect on the population dynamics, biochemical composition and fatty acid content of mixed microalgae grown in Anaerobic Liquid Digestate (ALD). Overall, 3.1 mM FeSO4 addition into ALD increased the total protein content 60% and led to highest biomass (1.56 g L−1) and chlorophyll-a amount (18.7 mg L−1) produced. Meanwhile, 0.4 mM MgSO4 addition increased the total carotenoid amount 2.2 folds and slightly increased the biomass amount. According to the microbial community analysis, Diphylleia rotans, Synechocystis PCC-6803 and Chlorella sorokiniana were identified as mostly detected species after confirmation with 4 different markers. The abundance of Chlorella sorokiniana and Synechocystis PCC-6803 increased almost 2 folds both in iron and magnesium addition. On the other hand, the dominancy of Diphylleia rotans was not affected by iron addition while drastically decreased (95%) with magnesium addition. This study helps to understand how the dynamics of symbiotic life changes if macro elements are added to the ALD and reveal that microalgae can adapt to adverse environmental conditions by fostering the diversity with a positive effect on high value product.

Effect of Low Temperature on Chlorophyll and Carotenoid Content on the Seedlings of Some Selected Boro Rice Varieties

A study was conducted to screen out the low-temperature tolerant Boro rice seedlings from November 2012 to January 2013 for facing the upcoming rice production challenge in Northwest Bangladesh. The experimental time was characterized by a prevailing low environmental temperature of below 15°C. Five rice cultivars (V1: BR-2; V2: BR-16; V3: Pariza; V4: Minicate; V5: BRRI dhan 50) were selected for the study. The leaf proline, chlorophyll content and total carotenoid content were investigated. The V2 (BR-16) seedling synthesized the higher leaf proline (1.228 g·g-1) at a low temperature than those of other tested cultivars. Again, the highest amount of chlorophyll-a (3.957 g·g-1), chlorophyll-b (2.118 g·g-1), chlorophyll-a/b ratio (3.6754 mg·g-1) and total chlorophyll (5.051 g·g-1) was measured in V2 (BR-16). The maximum total carotenoid (1.213 g·g-1) was also observed in V2. In this experiment, the V2 (BR-16) showed comparatively better potentiality to survive at low temperatures (below 15°C) than other varieties.

The effect of the epiphytes of Chaetomorpha crassa on the total chlorophyll-a and growth of Gracilaria verrucosa

Gracilaria belongs to the class of red algae (Rhodophyta), it has many subtypes with different morphology and anatomy. The epiphytes that often attack Gracilaria are Chaetomorpha crassa epiphytes; they trap Gracilaria and have a negative effect such as nutrient competition, inhibiting the light penetration and reducing the growth rate. The purpose of this study was to determine the effect of Chaetomorpha crassa epiphytes on the amount of chlorophyll-a and the growth of Gracilaria verrucosa. The treatment used was an epiphyte mass of Chaetomorpha crassa by as much as 0% (control), 25%, 50%, 75% and 100% of the mass of Gracilaria verrucosa. The analysis of the data used Analysis of Variants (ANOVA) and continued with further tests using Duncan's multiple range test. The results showed that Chaetomorpha crassa had no effect on the amount of chlorophyll-a, but that it affected the absolute weight growth and daily growth rate. The amount of chlorophyll-a, absolute weight growth, daily growth rate, and best long growth were obtained in treatment A (control). The amount of chlorophyll-a, absolute weight growth, and daily growth rate was 0.3110 μg / ml, 0.93 gram, and 1.68% per day respectively.

KUALITAS AIR SUNGAI PITAP BERDASARKAN INDEKS AUTOTROPIK DI KALIMANTAN SELATAN

An epilithic benthic algae functions as a primary producer living on rock substrates. The Autotrofic Index (AI) was measured to understand the level of water pollutions by quantifying an autotrofy organism. This research determined water quality of Pitap river based on the autotrofic level of an epilithic benthic algae and to understand the correlation between the autotrofic level and physicochemical parameters. The measurement of water quality based on Ash-Free Dry Mass (AFDM), the amount of chlorophyll-a and physicochemical parameters were taken every month with two repetitions on September and October 2014. Five sampling sites were selected using a purposive sampling method based on the variety of environmental conditions around the Pitap river. The results showed that the water quality in the upstream (Ajung and Langkap villages) were classified non-pollution (dominated by autotrof compounds) until less pollution (dominated by autotrof-heterotof compounds). Other locations in downstream (Tebing Tinggi, Sungsum and Juuh villages) were determined pollution of water quality by dominance of autotrof-heterotrof components to dominance by heterotof components. Spearman correlation explained that autotrofic index on September 2014 was closely correlated with water temperature and water velocity with their value are 0,954 and -0,794 at significantly α=0,01. On October 2014 water temperature was closely correlated with its value 0,681 at significantly α=0,05.