Amount Of Caffeine Research Articles

Quantitative Determination of Caffeine of Green Tea Seed Ethanol Extract on Anti-obesity in C57BL/6 Mice Fed a High-fat Diet and 3T3-L1 Cells

The study was carried out to examine the anti-obesity effects of 40% ethanol extract from green tea seed (GS) and quantitative determination of caffeine as its major compound. The specificity was satisfied with retention time and UV spectrum by analysis of caffeine using HPLC and comparison with standard compound. It showed a high linearity in the calibration curve with a coefficient of correlation (R 2 ) of 0.9974. The amount of caffeine in GS was about 4.649 mg/g (0.465%) in the three times analysis, and relative standard deviation (RSD) was less than 0.452% by the validated method. The anti-obesity effects of GS were evaluated by using Oil Red O staining in 3T3-L1 adipocytes and body weight, visceral fat and lipid profiles in high fat diet (HFD)-induced C57BL/6 obese mice. Our results indicated that treatment with GS dose-dependently decreased lipid accumulation contents (p<0.001). Moreover, after oral administration for 30 days feeding with HFD-induced obses mice, GS (100 and 300 mg/kg/day) produced a significant decrease in serum total cholesterol (TC), glutamic oxaloacetic transaminase (GOT), glutamic pyruvic transaminase (GPT) and visceral fat. Thus, the result of this study indicate that the GS may be a useful resource for the management of obesity.

Quantitative Determination of Caffeine of Green Tea Seed Ethanol Extract on Anti-obesity in C57BL/6 Mice Fed a High-fat Diet and 3T3-L1 Cells

The study was carried out to examine the anti-obesity effects of 40% ethanol extract from green tea seed (GS) and quantitative determination of caffeine as its major compound. The specificity was satisfied with retention time and UV spectrum by analysis of caffeine using HPLC and comparison with standard compound. It showed a high linearity in the calibration curve with a coefficient of correlation (R²) of 0.9974. The amount of caffeine in GS was about 4.649 mg/g (0.465%) in the three times analysis, and relative standard deviation (RSD) was less than 0.452% by the validated method. The anti-obesity effects of GS were evaluated by using Oil Red O staining in 3T3-L1 adipocytes and body weight, visceral fat and lipid profiles in high fat diet (HFD)-induced C57BL/6 obese mice. Our results indicated that treatment with GS dose-dependently decreased lipid accumulation contents (p0.001). Moreover, after oral administration for 30 days feeding with HFD-induced obses mice, GS (100 and 300 mg/kg/day) produced a significant decrease in serum total cholesterol (TC), glutamic oxaloacetic transaminase (GOT), glutamic pyruvic transaminase (GPT) and visceral fat. Thus, the result of this study indicate that the GS may be a useful resource for the management of obesity.

Fast analysis of caffeine in beverages and drugs by paper spray tandem mass spectrometry.

A simple and fast method based on paper spray mass spectrometry for the determination of caffeine in commercial beverages and drugs has been developed; the analyses were carried out in MRM mode, monitoring the transitions m/z 195 → m/z 138 for caffeine and m/z 198 → m/z 140 for the labeled internal standard. To verify the reliability of the proposed approach, a spiked sample (soda drink and paracetamol tablet) with a known amount of caffeine has been prepared and analyzed by PS-MS, providing accuracy values about 100%; the LOQ and LOD values were calculated at 1.2 and 1.6μg/mL, respectively. Both beverages and drugs were also analyzed with the classic analytical method based on LC-UV measurements, showing consistent results between the two approaches, thus confirming the reliability of the developed ambient MS determination. Graphical Abstract The assay of caffeine by paper mass spectrometry.

Energy drink consumption, health complaints and late bedtime among young adolescents.

Energy drinks contain large amounts of caffeine. Their effects on adolescent well-being are poorly known. We examined the relationship of energy drink consumption with health complaints and late bedtime among 13-year-olds. A classroom survey was conducted on all 7th graders in the Helsinki metropolitan region, Finland (73% responded; n=9446). Logistic regression analysis and structural equation modeling (SEM) were used. The prevalence of health complaints increased as energy drink consumption increased from non-consumers to several times/day. Late bedtime (≥11 PM) increased correspondingly. Those consuming energy drinks several times/day exhibited increased odds of experiencing daily health complaints compared with non-consumers: headache OR (adjusted)=4.6 (2.8-7.7), sleeping problems OR=3.6 (2.2-5.8), irritation OR=4.1 (2.7-6.1), tiredness/fatigue OR=3.7 (2.4-5.7), and late bedtime OR=7.8 (5.7-10.9). In SEM, energy drink consumption had a direct effect on health complaints and late bedtime and an indirect effect on health complaints via late bedtime. Energy drinks, late bedtime, and health complaints form a behavior pattern that is worth considering in schools, home and clinical settings when adolescents complain about headaches, problems with sleeping and corresponding symptoms.

Chemical composition in wild ethiopian Arabica coffee accessions

The exploration of accessions originating from Ethiopia is one option available to increase the genetic basis of new coffee cultivars. The aim of this study was to describe the chemical composition variability of Ethiopian coffee accessions maintained in Londrina and to evaluate the year effect on that. We observed no significant interaction between the years of cultivation and the amounts of caffeine, protein, lipids, chlorogenic acids, sucrose, cafestol and kahweol, thereby suggesting that evaluations of one year were reliable to describe this collection. All compounds showed variability, but the highest variability was found in cafestol and kahweol (32.81 and 34.45 %, respectively). Significant correlations among caffeine, chlorogenic acids and protein as well as between sucrose and lipids were observed. A significant negative correlation was found between kahweol and cafestol, and kahweol had positive and negative correlation with caffeine and chlorogenic acids, respectively. Hierarchical clustering analyses identified groups in the population, and the diversity found in one crop year was similar to that for 3 years. Greater diversity of composition was observed among the Western accessions compared to the ones from the East, but no clear separation was found between the accessions of these regions. These accessions are potential sources to increase the genetic diversity to obtain new coffee cultivars.

General and Genetic Toxicology of Guayusa Concentrate (Ilex guayusa).

Tea from the leaves of guayusa (Ilex guayusa) has a long history of consumption by Ecuadorian natives in regions where the plant is indigenous. The tea contains the methylxanthines caffeine and theobromine as well as chlorogenic acids, flavonoids, and sugars. Various studies were performed to evaluate the general and genetic toxicology of a standardized liquid concentrate of guayusa (GC). Guayusa concentrate was found to be negative in in vitro genotoxicity tests including the Ames test and a chromosome aberration study in human lymphocytes. The oral median lethal dose (LD50) of GC was >5,000 mg/kg for female rats. Guayusa concentrate was administered to male and female rats in a 90-day subchronic study at 1,200, 2,500, and 5,000 mg/kg/d of GC and a caffeine-positive control at 150 mg/kg/d corresponding to the amount of caffeine in the high-dose GC group. Effects observed in the GC-treated groups were comparable to those in the caffeine control group and included reductions in body weights, food efficiency, triglycerides values, and fat pad weights and increases in blood chemistry values for serum aspartate aminotransferase, serum alanine aminotransferase, and cholesterol and adaptive salivary gland hypertrophy. No signs of incremental toxicity due to any other components of guayusa were observed. The studies indicate no harmful effects of GC in these test systems.

The Determination of Caffeine Level of Wolaita Zone, Ethiopia Coffee Using UV-visible Spectrophotometer

Coffee is an important commodity culturally, commercially and economically in the world. Because of its consumption in most countries in the world, it is important to investigate the exact amounts of its chemical constituents. Caffeine is one of the main components of coffee that affect the quality of coffee. In this research, rapid, simple, accurate, reproducible and cheap UV-Vis spectrometer was used for the quantification of caffeine in coffee and dichloromethane was used as a solvent for the coffee samples taken from Wolaita zone of five woredas. The results of this research show that the amount of caffeine of the coffee samples ranged from (450.13 to 579.60 (mg/L). The amounts of caffeine in Bolso Bomba coffee is the greatest, of all the other coffee samples studied in Wolaita Zone. On the other hand, Kindo Koisha and that of Bolso sore coffee has the least caffeine content compared to the other coffee samples measured. The caffeine content of Sodo Zuria coffee and Humbo coffee samples has an average amounts of caffeine.

Habitual caffeine use in psychiatric patients: relationship with sleep quality and symptom severity

Objective: The purpose of this study was to investigate the patterns of caffeine use and the one-month prevalence of caffeine intoxication among psychiatric patients in comparison with healthy controls. Methods: Four hundred and one patients with various psychiatric disorders and 150 healthy controls were screened for current (one month) caffeine intoxication according to the Diagnostic and Statistical Manual of Mental Disorders, fourth edition. All parti-cipants were asked to complete Pittsburgh Sleep Quality Index (PSQI). The patients were also assessed with the Clinical Global Impression Scale (CGI) to determine symptom severity. Results: The amount of daily caffeine con-sumption was statistically significantly higher in healthy control subjects than in patients. However, the prevalence of caffeine intoxication was greater among patients with a psychiatric disorder (8%) when compared with healthy controls (2.7%). In the patients, the amount of caffeine consumption correlated positively with age, CGI, and PSQI scores, indicating that patients with older age, poorer sleep quality, and more severe pathology consumed higher amounts of caffeine. Conclusions: Caffeine intoxication was more prevalent in psychiatric patients than in healthy subjects. The amount of caffeine intake was shown to be associated positively with the severity of pathology and inversely with sleep quality. Further studies are needed to investigate the effect of regulating caffeine consumption on severity of pathology and sleep quality among psychiatric patients.

Variation in Caffeine Concentration Among Different Weight Loss Supplements Containing Green Tea and Green Coffee Extracts

Many dietary supplements have caffeine as an active ingredient. Because they are supplements, they are not required by the FDA to state the concentration of caffeine they contain. The purpose of this study was to find the actual amount of caffeine in various supplements, specifically those that include green tea and green coffee extract. We analyzed the dietary supplements, green coffee, and green tea by high-pressure liquid chromatography to compare the amounts of caffeine in each. We found that the amount of caffeine in all of the supplements tested was higher than the predicted value. This was expected as extracts should be more concentrated than their original substance. In addition, the supplements differed from each other in their caffeine content, with a range of 5.33 to 67.2 mg/serving in green coffee–based supplements and a range of 2.97 to 12.85 mg/serving in green tea–based supplements. An ANOVA test revealed significant differences between brands in most pairings and a t-test revealed significant differences between expected caffeine content and actual caffeine content found by HPLC. This supports our hypothesis that the resulting extracts from different preparation methods can vary significantly in caffeine levels. This variation may raise health and safety concerns as the ingredients list does not tell consumers how much caffeine they are ingesting.

Study on Roasted Date Seed Non Caffeinated Coffee Powder as a Promising Alternative

Although some amount of caffeine is good for health it has many serious health risks such as high blood pressure, hypertension among young adults, gout flare up, insomnia, indigestion, infertility, inhibition of collagen production in skin etc., Caffeine is an addictive drug whose consumption must be limited. Hence an alternate natural substance that looks tastes and smells like coffee that has no health risk is very much needed. Roasted date palm seed is taken as a substitute or coffee in this study. Date seed coffee powder is manufactured by washing, drying and roasting it for 30 minutes at 125°C. This date seed coffee powder was analysed using FTIR spectroscopy and it revealed that it contains 0% caffeine (indicted by the absence of peaks 1600 and 1800 cm-1). This paper indicates that the 9% roasted date seed powder can be used as a suitable alternate for coffee.

CHANGES THE STRUCTURE AND CAFFEINE ADSORPTION PROPERTY OF CALCINED MONTMORILLONITE

Montmorillonite is widely used as a natural adsorbent. It is generally considered that theadsorption properties of montmorillonite are largely due to its characteristic layer structure. The structure ofmontmorillonite changes under different temperature conditions. In this study, changes in the structure ofmontmorillonite with temperature are analyzed using Synchrotron X-Ray Powder Diffraction (SR-XRD).Observations are made on how montmorillonite with a modified structure is affected by the adsorption ofnonionic organic compounds in a water system. The object of this study is to elucidate the mechanism bywhich montmorillonite adsorbs nonionic organic compounds. It is confirmed that, without calcinationtreatment, adsorption of caffeine on montmorillonite changes its layer structure. Calcination treatment atabove 573 K causes irreversible dehydration in the structure of montmorillonite. Samples of montmorillonitecalcined at 673 K and 873 K show a reduction in the amount of caffeine adsorbed. In addition, calcinationtreatment of montmorillonite at 673 K and 873 K causes less intercalation into the layers. These results showthat adsorption of caffeine on montmorillonite without calcination treatment occurs with the intercalation ofcaffeine between layers of montmorillonite. On the other hand, montmorillonite calcined at 873 K isconfirmed to adsorb approximately 20 % of caffeine compared to untreated montmorillonite. It suggested thepossibility of the adsorption of caffeine even on the surfaces and end faces of layers of montmorillonite.

Substance use and misuse in burn patients: Testing the classical hypotheses of the interaction between post-traumatic symptomatology and substance use

ABSTRACTThe authors aimed to test whether the three classical hypotheses of the interaction between post-traumatic symptomatology and substance use (high risk of trauma exposure, susceptibility for post-traumatic symptomatology, and self-medication of symptoms), may be useful in the understanding of substance use among burn patients. Substance use data (nicotine, alcohol, cannabis, amphetamines, cocaine, opiates, and tranquilizers) and psychopathology measures among burn patients admitted to a burn unit and enrolled in a longitudinal observational study were analyzed. Lifetime substance use information (n = 246) was incorporated to analyses aiming to test the high risk hypothesis. Only patients assessed for psychopathology in a 6-month follow-up (n = 183) were included in prospective analyses testing the susceptibility and self-medication hypotheses. Regarding the high risk hypothesis, results show a higher proportion of heroin and tranquilizer users compared to the general population. Furthermore, in line with the susceptibility hypothesis, higher levels of symptomatology were found in lifetime alcohol, tobacco, and drug users during recovery. The self-medication hypothesis could be tested partially due to the hospital stay “cleaning” effect, but severity of symptoms was linked to the amount of caffeine, nicotine, alcohol, and cannabis use after discharge. It was found that the 3 classical hypotheses could be used to understand the link between traumatic experiences and substance use explaining different patterns of burn patient's risk for trauma exposure and emergence of symptomatology.

A survey of energy drink and alcohol mixed with energy drink consumption.

BackgroundEnergy drink consumption among youth is increasing despite recommendations by the American Academy of Pediatrics to eliminate consumption by youth. This study provides information on consumption of energy drinks and alcohol mixed with energy drinks (AmED) in a sample of Israeli youth and how consumer knowledge about the risks affects consumption rates.MethodsThe study was conducted in three Tel Aviv public schools, with a total enrollment of 1,253 students in grades 8 through 12. Among them, 802 students completed a 49-item questionnaire about energy drink and AmED consumption, for a 64 % response rate Non-responders included 451 students who were absent or refused to participate. All students in the same school were administered the questionnaire on the same day.ResultsEnergy drinks are popular among youth (84.2 % have ever drunk). More tenth through twelfth grade students consumed energy drinks than eighth and ninth grade students. Students who began drinking in elementary school (36.8 %) are at elevated risk for current energy drink (P < .001) and AmED (P = .002) use. Knowledge about amounts consumed and recommended allowances is associated with less consumption (OR 1.925; 95 %CI 1.18–3.14).DiscussionThe association between current AmED consumption and drinking ED at a young age is important. Boys and those who start drinking early have a greater risk of both ED and AmED consumption. The characteristics of early drinkers can help increase awareness of potential at-risk youth, such as junior and senior high school students with less educated or single parents.ConclusionsRisks posed by early use on later energy drink and AmED consumption are concerning. We suggest that parents should limit accessibility. Increased knowledge about acceptable and actual amounts of caffeine in a single product might decrease consumption.

Multivariate optimization of residual caffeine extraction from decaffeinated coffee

This study evaluated techniques of extraction of caffeine from decaffeinated coffee samples for application in quality control of industrial decaffeination processes. The extraction was studied using two methods, an aqueous one and another through liquid-liquid partition with chloroform. The objective was to extract the maximum amount of caffeine with the minimum of interference from the matrix and with good repeatability of extraction. After comparing the aqueous extraction and extraction with chloroform, a 23 factorial design was performed to optimize the liquid-liquid extraction. The parameters analyzed in the factorial design were the solvent:sample ratio, extraction time, and filtration or not after extraction. The optimum extraction point was defined using the amount of caffeine extracted as the response factor. Caffeine levels were quantified by capillary electrophoresis according to an analytical method previously optimized and validated. The best extraction condition was achieved through liquid-liquid partition with chloroform, using 30 mL of solvent, 7 min of agitation, and without filtration. This condition showed good repeatability (2.8%, n = 7), recovery of 96.7-107.4%, and removal of interfering matrix. The method was applied to samples of decaffeinated roasted and ground coffee, and instant coffee.

Quantitative Analysis of Active Pharmaceutical Ingredients (APIs) Using a Potentiometric Electronic Tongue in a SIA Flow System

AbstractAn advanced potentiometric electronic tongue and Sequential Injection Analysis (SIA) measurement system was applied for the quantitative analysis of mixtures containing three active pharmaceutical ingredients (APIs): acetaminophen, ascorbic acid and acetylsalicylic acid, in the presence of various amounts of caffeine as interferent. The flow‐through sensor array was composed of miniaturized classical ion‐selective electrodes based on plasticized PVC membranes containing only ion exchangers. Partial Least Squares (PLS) analysis of the steady‐state sensor array responses, measured in API mixtures prepared by the SIA system permitted a correct quantitative analysis of acetylsalicylic acid and ascorbic acid. Further optimization using multiway PLS fed by dynamic responses without additional feature extraction did not improve significantly the resolution of acetaminophen. Lastly, the chemometric treatment, involving the extraction of dynamic components of the transient response employing the Wavelet transform, the removal of less‐significant coefficients by means of Causal Index pruning and training of an Artificial Neural Network (ANN) with the selected coefficients, allowed the simultaneous determination of all the three studied APIs, while counterbalancing any interference due to caffeine.

Tobacco Metabolites and Caffeine in Human Milk Purchased via the Internet.

Chemicals inhaled or ingested by mothers can be present in their milk. Our objective was to determine levels of nicotine, cotinine, and caffeine in human milk purchased via the Internet. We purchased human milk (n=102) via the Internet and abstracted seller advertisements for information volunteered about tobacco and caffeine use. Nicotine, cotinine, and caffeine levels in the milk were quantified by mass spectrometry according to published protocols. No sellers indicated smoking in their advertisement. Many of the milk samples (58%) had detectable nicotine or cotinine; four (4%) of the samples had nicotine or cotinine levels high enough to indicate active smoking. Twelve (12%) sellers said in their advertisements that they specifically limit (4%) or avoid (8%) caffeine entirely. Five (5%) of the samples had caffeine levels consistent with consuming at least 1 cup of coffee 2 hours prior to milk expression. Detectable amounts of caffeine were found in almost all of the samples (97%). In 102 milk samples, we detected evidence of active smoking, secondhand smoke exposure, and almost ubiquitous caffeine consumption. Buyers of human milk on the Internet should be aware that advertisements do not always include accurate information as to what substances may be present. Sellers may misrepresent their health behaviors or be unaware of lifestyle factors that can lead to exposure to nicotine and caffeine.

A single serving of caffeinated coffee impairs postprandial glucose metabolism in overweight men.

Previous studies regarding the acute effects of coffee on glycaemic control have used a single large dose of coffee, typically containing the caffeine equivalent of 2-4 servings of coffee. This study investigates whether the acute effects of coffee are dose-dependent, starting with a single serving. A total of ten healthy overweight males participated in a two-part randomised double-blind cross-over study. In the first part, they ingested 2, 4 or 8 g instant decaffeinated coffee (DC) dissolved in 400 ml water with caffeine added in proportion to the DC (total 100, 200 or 400 mg caffeine) or control (400 ml water) all with 50 g glucose. In the second part, they ingested the same amounts of DC (2, 4, 8 g) or control, but with a standard 100 mg caffeine added to each. Capillary blood samples were taken every 15 min for 2 h after each drink and glucose and insulin levels were measured. Repeated measures ANOVA on glucose results found an effect when caffeine was varied in line with DC (P=0·008). Post hoc analysis revealed that both 2 and 4 g DC with varied caffeine content increased the glycaemic response v. There was no effect of escalating doses of DC when caffeine remained constant at 100 mg. These results demonstrate that one standard serving of coffee (2 g) is sufficient to affect glucose metabolism. Furthermore, the amount of caffeine found in one serving (100 mg) is sufficient to mask any potential beneficial effects of increasing other components. No dose-dependent effect was found.

Coffee consumption and propensity to experience aggressive feelings in provoking and frustrating situations

The present study focused on examining whether coffee intake is related to the intensity of aggressive feelings in reaction to various provoking or frustrating situations, along with consideration of sex and culture. Previous studies focused mainly on the dose of caffeine (not the habit of coffee drinking) and aggressive behavior. Many of these studies showed a positive relation between the amount of caffeine consumed and aggression, so it was expected that also habitual coffee consumption would be positively related to propensity for aggressive feelings &lt;i&gt;&lt;i&gt;&lt;i&gt;&lt;i&gt;&lt;i&gt;&lt;i&gt;in situ&lt;/i&gt;&lt;/i&gt;&lt;/i&gt;&lt;/i&gt;&lt;/i&gt;&lt;/i&gt;ations of provocation and frustration. Participants from Greece (n = 299) and Poland (n = 300) declared whether they were coffee drinkers (at least 1 mug of coffee per day) and completed the Situational Triggers of Aggressive Responses questionnaire. The results showed that in females, coffee drinkers had higher scores in Sensitivity to Provocation (SP) and Frustration (SF) than nondrinkers, while no differences between drinkers and nondrinkers appeared in males. Moreover, amongst coffee drinkers, females had higher scores in SP and SF than males, while no sex differences were found in nondrinkers. Furthermore, in Poland coffee drinkers had higher SP scores than nondrinkers. Poles also had higher SP scores than Greeks, but only when coffee drinkers were compared.

Associations Between Dietary Intake And 161-km Race Performance, Fatigue, And Muscle Soreness

Ultra-marathons (>42.2km) are increasingly popular in the US, but there is a paucity of empirical data regarding the effects of diet on ultra-endurance performance and associated variables. PURPOSE: To characterize the dietary intake of ultra-marathoners, and to determine the relationships between diet and 100 mile run performance, muscular soreness, and fatigue. METHODS:Participants (n=19) were registrants of the Mohican 100 mile Trail Run held in Loudonville, OH. Two weeks prior to the event, participants recorded three days of dietary intake representing their typical diet, which was completed online using the validated ASA24, and submitted a race-diet plan. Participants reported perceived muscle soreness and fatigue every 25 miles, and 24 and 48hr post-race using a visual analogue scale (VAS). Non-paired t-tests were used to compare caloric and macronutrient intake during training between finishers and non-finishers. Pearson’s correlations were performed to determine if absolute or relative macronutrient intake or caloric intake were associated soreness and fatigue. RESULTS: Males (n=15) consumed 386±76.7g carbohydrate (CHO), 136±32.3g protein (PRO), 118±37.2g fat (FAT), and 3121±579.2 total kcals per day during training. Females (n=4) consumed 270±39.6g CHO, 81.9±22.3g PRO, 97.4±37.6g FAT, and 2221±482.1 kcals per day. Overall, participants’ training diet was comprised of 49±.06% CHO, 16±.03% PRO, and 34±.06% FAT. Finishers (n=15) consumed significantly more CHO (360±86.3, p=0.04) than non-finishers (n=4), but no differences were found in fat, protein or total caloric intake between finishers and non-finishers. Participants who consumed more fat during the race reported higher fatigue at 25 miles (r=0.67, p=0.04), and those who consumed higher amounts of caffeine reported higher soreness at 50 miles (r=0.80; p=0.03). Race intake of CHO and total kcal were not associated with soreness or fatigue (p>0.05). CONCLUSIONS: These preliminary results indicate that CHO intake during training is associated with finishing a 100-mile race; however, ultra-marathon runners may not consume adequate CHO. Higher fat and caffeine intake during ultra-marathons may be associated with muscular fatigue and soreness, but further research is needed to confirm these relationships.

The effect of different doses of caffeine on cardiovascular variables and shooting performance

Summary Study aim: the purpose of this study was to assess the effect of 3 mg and 5 mg per kg of body weight of caffeine on heart rate, blood pressure and shooting performance among elite shooters. Material and methods: sStudy participants comprised 8 male shooters among athletes at the national level with at least 2 years of experience, with the mean age (26.50±13.08 years), weight (73.02 ± 12.2 kg), height (174.62 ± 8.97 cm) and BMI (23.93 ± 2.88 kg/m2). The blood pressure and heart rate of all participants were measured at rest. Participants then randomly took caffeine (3 mg and 5 mg per kg of body weight) or placebo for 3 different days, 2 days apart. One hour after ingestion, they shot with rifle and air pistol. Statistical analysis was performed using the ANOVA with repeated measures and the Bonferroni test. Results: the results of the survey showed that taking 5 mg/kg of caffeine caused a significant increase in systolic blood pressure (p &lt; 0.001), diastolic blood pressure (p &lt; 0.05) and also caused a significant increase in heart rate (p &lt; 0.05) and a significant decrease in shooting performance (p &lt; 0.05). Moreover, taking 3 mg/kg of caffeine caused a significant increase in systolic blood pressure (p &lt; 0.05). But this amount of caffeine had no significant effect on the heart rate, diastolic blood pressure or shooting performance (p &lt; 0.05). Conclusions: the results of this study suggest that taking 5 mg/kg of caffeine can increase the blood pressure and heart rate of the shooters that leads to a decrease in shooting performance.