BACKGROUND: When creating models utilizing artificial neural networks, the quantity of training data and the distribution of data need to be considered, particularly when making gender predictions. AIM: This study seeks to determine the potential impact of using the synthetic minority oversampling technique (SMOTE) on gender prediction using the artificial neural networks model. MATERIALS AND METHODS: The current study utilized a dataset consisting of 297 cephalometric measurements from Indonesian patients, comprising 229 samples from females and 68 samples from males. WebCeph is used to measure certain parameters, such as Sella-Nation-Point A (SNA) angle, mandibular length, mandibular angle, Sella-Glabella-Point A (SGA) angle, and diagnosis. Data processing and artificial neural networks model creation were conducted using Python. RESULTS: The gender identification accuracy of the artificial neural networks model is 87% for females and 0% for males, resulting in an overall average accuracy of 78%. When using SMOTE, the accuracy is 22%, with 0% for females and 37% for males. However, when using SMOTE and normalization, the accuracy increases to 71%, with 82% for females and 30% for males. The accuracy of normalization without SMOTE is 76%, with 86% for females and 14% for males. CONCLUSIONS: This research has proven the efficacy of SMOTE in improving the classification of male matrices. Nevertheless, this study reveals that the overall accuracy results of SMOTE are suboptimal in comparison to the absence of SMOTE and normalization. The application of data balancing strategies is necessary to achieve optimal accuracy in gender prediction when artificial neural networks, and other parameters must be applied.