Amount Of Particulate Organic Matter Research Articles

Land use impact on carbon mineralization in well aerated soils is mainly explained by variations of particulate organic matter rather than of soil structure

Abstract. Land use is known to exert a dominant impact on a range of essential soil functions like water retention, carbon sequestration, organic matter cycling and plant growth. At the same time, land use management is known to have a strong influence on soil structure, e.g., through bioturbation, tillage and compaction. However, it is often unclear whether the differences in soil structure are the actual cause of the differences in soil functions or if they only co-occur. This impact of land use (conventional and organic farming, intensive and extensive meadow, extensive pasture) on the relationship between soil structure and short-term carbon mineralization was investigated at the Global Change Exploratory Facility, in Bad Lauchstädt, Germany. Intact topsoil cores (upper 10 cm, n=75) were sampled from all land use types at the early growing season. Soil structure and microbial activity were measured using X-ray-computed tomography and respirometry, respectively. Differences in microstructural properties between land uses were small in comparison to the variation within land uses. The most striking difference between land uses was larger macropore diameters in grassland soils due to the presence of large biopores that are periodically destroyed in croplands. Grasslands had larger amounts of particulate organic matter (POM), including root biomass, and also greater microbial activity than croplands, both in terms of basal respiration and rate of carbon mineralization during growth. Basal respiration among soil cores varied by more than 1 order of magnitude (0.08–1.42 µg CO2-C h−1 g−1 soil) and was best explained by POM mass (R2=0.53, p<0.001). Predictive power was only slightly improved by considering all bulk, microstructure and microbial properties jointly. The predictive power of image-derived microstructural properties was low, because aeration did not limit carbon mineralization and was sustained by pores smaller than the image resolution limit (<30 µm). The frequently postulated dependency of basal respiration on soil moisture was not evident even though some cores were apparently water limited, as it was likely disguised by the co-limitation of POM mass. This finding was interpreted in regards to the microbial hotspots which form on decomposing plant residues and which are decoupled from water limitation in bulk soil. The rate of glucose mineralization during growth was explained well by substrate-induced respiration (R2=0.84) prior to growth, which in turn correlated with total microbial biomass, basal respiration and POM mass, and was not affected by pore metrics. These findings stress that soil structure had little relevance in predicting carbon mineralization in well-aerated soil, as mineralization appeared to by predominantly driven by the decomposition of plant residues in intact soil. Land use therefore affects carbon mineralization in well-aerated soil mainly in the amount and quality of labile carbon.

Effective Purification of Eutrophic Wastewater from the Beverage Industry by Microbubbles

Beverage industries often discharge large amounts of organic matter with their wastewater. Purification of the effluent is their obligation, but it is nontrivial. Among wastewater components, removal of dissolved organic matter often requires much effort. Therefore, a special effective technique must be considered. Microbubbles (1–100 μm) have several special properties of relevance to wastewater treatment. In this study, the effectiveness of microbubbles for treating and purifying beverage wastewater was evaluated. Orange juice, lactic acid drink, and milk were used as model substrates of dissolved organic matter, and degradation experiments were carried out. Rates of air supply by microbubbles were 0.05% (air/wastewater) min−1. Results indicated that the total organic carbon (TOC) in an experimental vessel containing milk (high nitrogen content) decreased by 93.1% from 11.0 to 0.76 g during a 10-day incubation. The TOC of lactic acid drink (least nitrogen content) decreased by 66.3%, from 15.6 to 5.26 g, and the TOC of orange juice (medium nitrogen content) decreased by 82.7%, from 14.8 to 2.55 g. Large amounts of particulate organic matter floated on the water surface in the milk with microbubbles and were removed easily, while almost no floating materials were observed in the orange juice and lactic acid drink. In contrast, in the macrobubble treatment (diameter 0.1 to 2 mm), only 37.0% of TOC in the milk was removed. Whereas the macrobubble treatments were anaerobic throughout the incubations, the microbubble treatments returned to aerobic conditions quickly, and brought 10 times greater bacterial abundances (>108 cells mL−1). These results suggest that microbubbles are much superior to macrobubbles in supplying oxygen and accelerating the growth of aerobic bacteria, and that wastewater containing more nitrogenous compounds was purified more effectively than that with less nitrogen by microbial degradation and floating separation.

Harpacticoid assemblages (Copepoda: Harpacticoida) in the hyporheic zone of four streams in central Slovenia

Harpacticoids are an important component of meiofaunal assemblages in hyporheic zone. The goal of this study was to investigate distribution patterns of interstitial harpacticoid assemblages from four pre-Alpine streams originating in the Dinaric Karst and flowing into the Ljubljanica River. The sampling was conducted in 2002 at 12 locations distributed at a distance of approximately 1 km along each stream including tributaries, at a depth of 30–60 cm in the wetted channel (three sites per location) and depths from 65 to 160 cm on the stream banks (one site per location) using a Bou-Rouch pump. Concurrently, the interstitial water’s physical and chemical parameters were measured at two sites within each location (streambed, streambank). A total of 24 harpacticoid species were found, 12 of which were stygobionts (i.e., species living exclusively in groundwaters). Among them, two previously unknown species for science were found. Harpacticoid assemblage composition, with the exception of those from the Iška stream, did not differ significantly between the streams, indicating interconnectivity of the interstitial milieu. Sediment structure, amounts of particulate organic matter, conductivity and redox conditions seemed to have certain impacts, indicating the importance of hydrological and geological settings for harpacticoid assemblages.

Sediment transport, turbidity, and dissolved oxygen responses to annual streambed drawdowns for downstream fish passage in a flood control reservoir

Sediment transport, turbidity, and dissolved oxygen were evaluated during six consecutive water years (2013–2018) of drawdowns of a flood control reservoir in the upper Willamette Valley, Oregon, USA. The drawdowns were conducted to allow volitional passage of endangered juvenile chinook salmon through the dam's regulating outlets by lowering the reservoir elevation to a point where the historical streambed was exposed and transported water and sediment through the reservoir dam. Sediment loads during the drawdown were highest in the first year of monitoring, with a computed value of 40,200 metric tons over a 5-day drawdown, followed by 5 years of lower sediment loads and lower sediment transport rates, suggesting that much of the stored sediment within the reservoir thalweg was transported out of the reservoir in the early years of the consecutive drawdowns. Suspended sediment concentrations (SSC) computed using turbidity and streamflow data resulted in maximum SSC at the onset of the drawdowns, with the highest computed values occurring during the water year 2017 drawdown at 17,500 mg/L (turbidity = 2,990 FNU), and average drawdown SSC values ranging from 654 to 3,950 mg/L for the six years of monitoring. Computed SSC were on the lower range of concentrations that could be harmful to out-migrating juvenile salmon published in other studies. High amounts of particulate organic matter and sand-sized material in drawdown SSC samples affected relations between turbidity and SSC, requiring the use of multiple surrogate regression models over short time frames. Dissolved oxygen minimum values were recorded in two of the monitoring years, with a minimum value of 0.71 and 3.4 mg/L recorded at the onset of the drawdowns in water years 2016 and 2018, respectively. Dissolved oxygen values below 4 mg/L lasted for 1 h, suggesting a rapidly expressed chemical oxygen demand. The response of suspended sediment loads and SSC highlight the site-specific nature of reservoir drawdowns, and the need for evaluation of expected sediment responses for drawdowns being considered at other locations.

The impact of particulate and soluble organic matter on physicochemical properties of extracellular polymeric substances in a microalga Neocystis mucosa SX

Microalgal harvesting is an important link for the integration microalgae-based wastewater treatment with biodiesel production. Extracellular polymAAeric substances (EPS) are considered to be an important factor affecting the flocculation of microalgae. Because the remarkable amount of particulate organic matter exists in real wastewater and is thought to be not readily biodegradable, the effect of particulate organic matter on EPS properties cannot be ignored. Nevertheless, the response of EPS in microalgae to particulate organic matter has still remained unclear. In the study, starch, glucose and sucrose were used to simulate particulate and soluble organic matter to investigate the effect of particulate and soluble organic matter on physicochemical properties of EPS in a microalga Neocystis mucosa SX. The results clearly showed that particulate organic matter significantly promoted the production of EPS, especially extracellular polysaccharides, in the microalga via the cell lysis and the stress induced by available carbon starvation. Compared to notable change on EPS content, the chemical structure and the monomer composition of extracellular polysaccharides did not significantly respond to particulate and soluble organic matter. However, the proportion of functional groups and monomers had a notable response. EPS in the microalga cultivated with particulate organic matter had a lower proportion of some hydrophilic groups C-OH and CO, compared to the microalga cultivated with soluble organic matter. The high content of extracellular polysaccharides and the low proportion of hydrophilic components in EPS as binding sites for the flocculation significantly reduced the flocculation efficiency of the microalga. The results were conducive to reveal the potential mechanism on the response of physicochemical properties of EPS to particulate and soluble organic matter in the process of microalgae-based wastewater treatment, which was essential for microalgal harvesting.

Sedimentología e icnología de un delta fluvio-dominado, Formación Lajas (Jurásico Medio), cuenca Neuquina, Argentina

Sedimentologic and ichnologic analysis of the Middle Jurassic Lajas Formation in Bajada de Los Molles area allow recognizing upper offshore-lower shoreface deposits in transition to prodelta, delta front and interdistributary bay succesions. This system is classified as a river-dominated delta due to the presence of distributary channel deposits with huge amounts of particulate organic matter and low diversity and abundance of trace fossils. Hyperpycnites are common in the basal and upper part of the studied section, and form channel-levee and distributary-channel systems, integrated by massive sandstones and load deformation structures, or transitional and recurrent passages of tractive sedimentary structures with abundant particulate organic matter on the foresets. The greatest diversity and abundance of trace fossils are recognized in the upper offshore-lower shoreface deposits where Skolithos and Cruziana ichnofacies occur. Deltaic deposits show trace fossil associations with lower diversity and abundance than the fully marine ones, whereas the hyperpycnite deposits are either unbioturbated or show the lowest diversity and abundance of trace fossils, reflecting the most stressed conditions within the system.

Divergent Capacity of Scleractinian and Soft Corals to Assimilate and Transfer Diazotrophically Derived Nitrogen to the Reef Environment.

Corals are associated with dinitrogen (N2)-fixing bacteria that potentially represent an additional nitrogen (N) source for the coral holobiont in oligotrophic reef environments. Nevertheless, the few studies investigating the assimilation of diazotrophically derived nitrogen (DDN) by tropical corals are limited to a single scleractinian species (i.e., Stylophora pistillata). The present study quantified DDN assimilation rates in four scleractinian and three soft coral species from the shallow waters of the oligotrophic Northern Red Sea using the 15N2 tracer technique. All scleractinian species significantly stimulated N2 fixation in the coral-surrounding seawater (and mucus) and assimilated DDN into their tissue. Interestingly, N2 fixation was not detected in the tissue and surrounding seawater of soft corals, despite the fact that soft corals were able to take up DDN from a culture of free-living diazotrophs. Soft coral mucus likely represents an unfavorable habitat for the colonization and activity of diazotrophs as it contains a low amount of particulate organic matter, with a relatively high N content, compared to the mucus of scleractinian corals. In addition, it is known to present antimicrobial properties. Overall, this study suggests that DDN assimilation into coral tissues depends on the presence of active diazotrophs in the coral’s mucus layer and/or surrounding seawater. Since N is often a limiting nutrient for primary productivity in oligotrophic reef waters, the divergent capacity of scleractinian and soft corals to promote N2 fixation may have implications for N availability and reef biogeochemistry in scleractinian versus soft coral-dominated reefs.

Slurry acidification and anaerobic digestion affects the speciation and vertical movement of particulate and nanoparticulate phosphorus in soil after cattle slurry application

Livestock slurry contains a large amount of particulate organic matter. When applied to soil these particles can act as carriers transporting phosphorus (P) through and over soils to watercourses. Little is known, however, about how slurry treatment (e.g. acidification and anaerobic digestion) may affect slurry particle size distribution and P forms within the particulate fraction. To characterize the small slurry particles, we separated untreated, acidified and anaerobically digested cattle slurry into 0.45–63 μm, <0.45 μm, <100 kDa and <3 kDa fractions and determined their P speciation. To understand the role of slurry particles on P leaching/translocation in soil, we applied the <63 μm, <0.45 μm and <100 kDa fractions of untreated and processed slurries to the surface of an unplanted agricultural, sand-textured soil in laboratory microcosms and studied P movement under a simulated rainfall regime. Results showed that the 0.45–63 μm particulate fraction accounted for >60% of total P content of the untreated and anaerobically digested cattle slurries. Acidification reduced the total P quantity of the 0.45–63 μm fraction by 26% through dissolution and desorption, resulting in a greater P in the <0.45 μm fraction. Anaerobic digestion increased the total P content of the 0.45–63 μm fraction, as the decomposition and breakdown of coarser particles transferred more P to this fraction. After application to soil, 3.3% of the added inorganic P and 1.7% of the added organic P was found in the leachate in the untreated <63 μm treatment. The 0.45–63 μm particulate fraction largely contributed to this P leaching, while the nanoparticulate fraction (3 kDa-0.45 μm) contained little P and had no appreciable effect on P leaching. Overall, acidification had no impact on P leaching. In contrast, anaerobic digestion increased inorganic P leaching by 67% and organic P leaching by 127%. In conclusion, our findings highlight the importance of the 0.45–63 μm fraction in P transport through soil, and the enhanced bioavailability and mobility of P following anaerobic digestion. These need greater consideration in future studies promoting the sustainable use of livestock waste and P recovery technologies.

Soil structure formation and organic matter distribution as affected by earthworm species interactions and crop residue placement

Earthworms play an important role in soil organic matter (SOM) dynamics and soil structure formation, including soil porosity and aggregate stability. Earthworms feed on organic inputs such as crop residues (CR) which are displaced by mouldboard ploughing. In a 61-day mesocosm experiment, we investigated the effects of CR placement (surface-applied vs. incorporated) and different earthworm species (combinations) on: 1) the survival and biomass of the earthworm species Lumbricus terrestris, L. rubellus, and Aporrectodea caliginosa, representing anecic, epigeic and endogeic ecological groups, respectively; and 2) earthworm-mediated soil structure formation. Earthworms were present either as single species or as species mixtures combining anecics with each of the other groups. Incorporating CR reduced biomass of surface-feeders (L. terrestris: −30% of initial body weight vs. −9% when CR were surface-applied; L. rubellus: −74% vs. −24%, respectively). L. rubellus survival was also lower when CR were incorporated (50%) than when CR were surface-applied (92%). In surface-applied CR treatments, the amount of particulate organic matter (POM) > 250 μm in the soil profile was positively affected by L. terrestris in the soil upper 20 cm by 16.5%. A similar but weaker effect was found when CR were incorporated (9% increase). Large water-stable macroaggregates (>2000 μm) increased in the upper 20 cm soil only when CR were surface-applied and L. terrestris was present (from 2.7 to 13.1 g kg−1). Small water-stable aggregates increased with functional groups interactions at all soil depths, irrespective of the CR placement. Surface-applied CR increased soil porosity at 2.5–10 cm depth. Large water-stable macroaggregate formation by earthworms was hampered through the incorporation of CR, although CR incorporation increased porosity between 2.5 and 30 cm soil depth despite reduced earthworm biomass. Furthermore, small macroaggregate formation was hampered by single species, whereas combining functional groups stimulated their formation. Under field conditions residue incorporation might result in trade-offs between the contribution of surface-feeding earthworms to soil porosity and i) their fitness, as surface-feeding earthworms' body weight loss was larger than when crop residues were surface-applied; as well as ii) large water-stable macroaggregates formation, as no increase in those was found when CR was incorporated.

Microscale Heterogeneity of the Spatial Distribution of Organic Matter Can Promote Bacterial Biodiversity in Soils: Insights From Computer Simulations.

There is still no satisfactory understanding of the factors that enable soil microbial populations to be as highly biodiverse as they are. The present article explores in silico the hypothesis that the heterogeneous distribution of soil organic matter, in addition to the spatial connectivity of the soil moisture, might account for the observed microbial biodiversity in soils. A multi-species, individual-based, pore-scale model is developed and parameterized with data from 3 Arthrobacter sp. strains, known to be, respectively, competitive, versatile, and poorly competitive. In the simulations, bacteria of each strain are distributed in a 3D computed tomography (CT) image of a real soil and three water saturation levels (100, 50, and 25%) and spatial heterogeneity levels (high, intermediate, and low) in the distribution of the soil organic matter are considered. High and intermediate heterogeneity levels assume, respectively, an amount of particulate organic matter (POM) distributed in a single (high heterogeneity) or in four (intermediate heterogeneity) randomly placed fragments. POM is hydrolyzed at a constant rate following a first-order kinetic, and continuously delivers dissolved organic carbon (DOC) into the liquid phase, where it is then taken up by bacteria. The low heterogeneity level assumes that the food source is available from the start as DOC. Unlike the relative abundances of the 3 strains, the total bacterial biomass and respiration are similar under the high and intermediate resource heterogeneity schemes. The key result of the simulations is that spatial heterogeneity in the distribution of organic matter influences the maintenance of bacterial biodiversity. The least competing strain, which does not reach noticeable growth for the low and intermediate spatial heterogeneities of resource distribution, can grow appreciably and even become more abundant than the other strains in the absence of direct competition, if the placement of the resource is favorable. For geodesic distances exceeding 5 mm, microbial colonies cannot grow. These conclusions are conditioned by assumptions made in the model, yet they suggest that microscale factors need to be considered to better understand the root causes of the high biodiversity of soils.

Climate effects of a hypothetical regional nuclear war: Sensitivity to emission duration and particle composition

AbstractHere, we use a coupled atmospheric‐ocean‐aerosol model to investigate the plume development and climate effects of the smoke generated by fires following a regional nuclear war between emerging third‐world nuclear powers. We simulate a standard scenario where 5 Tg of black carbon (BC) is emitted over 1 day in the upper troposphere–lower stratosphere. However, it is likely that the emissions from the fires ignited by bomb detonations include a substantial amount of particulate organic matter (POM) and that they last more than 1 day. We therefore test the sensitivity of the aerosol plume and climate system to the BC/POM ratio (1:3, 1:9) and to the emission length (1 day, 1 week, 1 month). We find that in general, an emission length of 1 month substantially reduces the cooling compared to the 1‐day case, whereas taking into account POM emissions notably increases the cooling and the reduction of precipitation associated with the nuclear war during the first year following the detonation. Accounting for POM emissions increases the particle size in the short‐emission‐length scenarios (1 day/1 week), reducing the residence time of the injected particle. While the initial cooling is more intense when including POM emission, the long‐lasting effects, while still large, may be less extreme compared to the BC‐only case. Our study highlights that the emission altitude reached by the plume is sensitive to both the particle type emitted by the fires and the emission duration. Consequently, the climate effects of a nuclear war are strongly dependent on these parameters.

Land use effects and stream metabolic rates: a review of ecosystem response

Abstract Aim To conduct a review of the literature in order to identify the general stream metabolic responses to land use change. Methods I conducted a scientometric review analyzing the distribution of the studies among different environments, the land use scale used, and the general trends in stream metabolism response under each kind of land-use impact. Major Results Most of the analyzed studies were conducted in temperate environments, studying land-use impacts at catchment scale. Ecosystem metabolism responded to land use impacts most of the cases, especially under agricultural pressure. The general responses to land-use alterations were increases in rates of Gross Primary Production (GPP) and ecosystem Respiration (R). Primary production increases were mostly related to light and nutrient concentration increases, while R was usually related to water nutrient concentration, temperature and amount of particulate organic matter, but this general behavior can change under high impact levels where sometimes GPP decreases in response to turbidity increases. Riparian vegetation restoration have a positive effect in driving stream metabolic conditions in the direction of pristine condition, but the effectiveness of this approach is reduced in highly impacted systems. Conclusions To elucidate the mechanistic relations between stream metabolic changes and land use impacts is still one fundamental aspect to study in order to best predict effects of land use changes and establish management and protection programs. Thus, studies should focus on the causative relations between stream processes and land use changes considering different scales and multiple stress scenarios in order to improve our understanding about factors that drive the observed metabolic changes.

Large amounts of labile organic carbon in permafrost soils of northern Alaska.

Permafrost-affected soils of the northern circumpolar region represent 50% of the terrestrial soil organic carbon (SOC) reservoir and are most strongly affected by climatic change. There is growing concern that this vast SOC pool could transition from a net C sink to a source. But so far little is known on how the organic matter (OM) in permafrost soils will respond in a warming future, which is governed by OM composition and possible stabilization mechanisms. To investigate if and how SOC in the active layer and adjacent permafrost is protected against degradation, we employed density fractionation to separate differently stabilized SOM fractions. We studied the quantity and quality of OM in different compartments using elemental analysis, 13 C solid-phase nuclear magnetic resonance (13 C-NMR) spectroscopy, and 14 C analyses. The soil samples were derived from 16 cores from drained thaw lake basins, ranging from 0 to 5500years of age, representing a unique series of developing Arctic soils over time. The normalized SOC stocks ranged between 35.5 and 86.2kgSOCm-3 , with the major amount of SOC located in the active layers. The SOC stock is dominated by large amounts of particulate organic matter (POM), whereas mineral-associated OM especially in older soils is of minor importance on a mass basis. We show that tremendous amounts of over 25kgOC per square meter are stored as presumably easily degradable OM rich in carbohydrates. Only about 10kgOC per square meter is present as presumably more stable, mineral-associated OC. Significant amounts of the easily degradable, carbohydrate-rich OM are preserved in the yet permanently frozen soil below the permafrost table. Forced by global warming, this vast labile OM pool could soon become available for microbial degradation due to the continuous deepening of the annually thawing active layer.

Effect of plant communities on aggregate composition and organic matter stabilisation in young soils

Objectives Carbon (C) content in pools of very young soils that developed during 45 years from loess was analysed in relation to vegetation: deciduous and coniferous forests and cropland. We hypothesised that variations in the amount of particulate organic matter (POM) can explain the C accumulation and also affects the C bound to mineral surfaces in soil under various vegetation.

Spatio-temporal variation of sediment transport in the Selenga River Basin, Mongolia and Russia

Many Asian rivers have been intensively used to boost economic growth, resulting in sudden and drastic changes in sediment transport patterns. However, a few rivers are still undisturbed. The present paper considers the unregulated Selenga River and its basin, located in Russia and Mongolia. The river contributes to 50 % of the total inflow to Lake Baikal. Pending scientific challenges include the quantification of sediment loads and erosion–deposition patterns along the Selenga River system, the understanding of suspended particulate matter composition and the importance of peak flow events for total sediment discharge and heavy metal transport. Field data and hydraulic modeling converge on showing that peak flow events during spring and summer contribute to the main part (70–80 %) of the annual sediment and pollution loads in upstream parts of the basin. The Selenga River carries mostly silt and sand. The average particle size differs by a factor of four between summer floods and base flow periods. The low amount of particulate organic matter (ranging between 1 and 16 % in the studied rivers) is consistent with the significant role of sediments originating from mining areas and in-channel sources. The bed load transport in the downstream part of the river basin is high (up to 50 % of the total transport), and channel storage plays an important role in the total sediment transport to Lake Baikal. Reported statistically significant multi-decadal declines in sediment fluxes in the downstream Selenga River can be attributed to the abandonment of cultivated lands and (most likely) to changing hydroclimatic factors.

Live weight and relative faecal production of Hylamorpha elegans (Burm.) (Coleoptera: Scarabaeidae) larvae fed on soils with different particulate organic matter content

AbstractPrevious studies using white grub larvae have not found a relationship between scarabaeid larval growth and the quantity of total organic matter (OM) in the feeding soil. This is probably because it is the type or quality of the OM that is more important to larval nutritional requirements. The aim of this research was to study, under laboratory conditions, how the quality of OM, measured as the percentage of particulate OM (POM), affected the live weight and faecal production of Hylamorpha elegans (Coleoptera: Scarabaeidae) larvae. Results showed that live weight gain of second and third instar larvae increased with an increase in the amount of POM. The faecal production showed no differences. Therefore, the effect on second and third instar larvae was related to the quality of the resource and not to an increase in the quantity of soil ingested. Better understanding of the dynamics of POM and other labile fractions of the soil OM as microbial biomass could be fundamental to assessing and implementing out agricultural practices that can ameliorate the impact of this pest.

Bacterial diversity in the bottom boundary layer of the inner continental shelf of Oregon, USA

There have been few studies of the bacterial community within the bottom boundary layer (BBL)—the turbulent region of the water column above the benthos—in shallow seas. Typi- cally, the BBL has large amounts of particulate organic matter suspended by turbulence, and it is often the first region of the water column to become hypoxic when oxygen declines. Communities at the surface (5 m) and in the BBL (1 to 10 m above the sea floor) were compared by terminal restric- tion fragment length polymorphism (T-RFLP) analysis and sequencing of the 16S rRNA gene. Multi- variate statistical methods (hierarchical clustering, non-metric multidimensional scaling, and analy- sis of similarity (ANOSIM)) indicated that the microbial community of the BBL is distinct from the surface community. ANOSIM supported the distinction between surface and BBLs (R values 0.427 and 0.463, based on analysis with restriction enzymes BsuR1 and Hin6I, respectively, p < 0.1%). Six terminal restriction fragments showed an increase in abundance with depth. Cloning, screening and sequencing identified these as a novel environmental clade (Eastern North Pacific Chromatiales (ENPC) clade), the ARTIC96BD-19 clade of Gammaproteobacteria, the 6N14 and Agg8 clades of the phylum Planctomycetes, the OM60/NOR5 clade of Gammaproteobacteria, and uncultivated mem- bers of the Roseobacter clade in the MB11C09 and ULA23 subgroups. To the best of our knowledge, this analysis is the first to focus on the unique composition of microbial communities of the BBL in shallow, inner-shelf regions off the coast of Oregon, USA, and the first to report that an uncharacter- ized clade of Chromatiales is indigenous in this habitat.

Effect of agitation on the performance of an anaerobic sequencing batch biofilm reactor in the treatment of dairy effluents

Agitation rate is an important parameter in the operation of Anaerobic Sequencing Biofilm Batch Reactors (ASBBRs), and a proper agitation rate guarantees good mixing, improves mass transfer, and enhances the solubility of the particulate organic matter. Dairy effluents have a high amount of particulate organic matter, and their anaerobic digestion presents inhibitory intermediates (e.g., long-chain fatty acids). The importance of studying agitation in such batch systems is clear. The present study aimed to evaluate how agitation frequency influences the anaerobic treatment of dairy effluents. The ASBBR was fed with wastewater from milk pasteurisation process and cheese manufacture with no whey segregation. The organic matter concentration, measured as chemical oxygen demand (COD), was maintained at approximately 8,000 mg/L. The reactor was operated with four agitation frequencies: 500 rpm, 350 rpm, 200 rpm, and no agitation. In terms of COD removal efficiency, similar results were observed for 500 rpm and 350 rpm (around 90%) and for 200 rpm and no agitation (around 80%). Increasing the system's agitation thus not only improved the global efficiency of organic matter removal but also influenced volatile acid production and consumption and clearly modified this balance in each experimental condition.

Filtration pressure by bivalves affects the trophic conditions in Mediterranean shallow ecosystems

Bivalve filtration may control the amount of seston in coastal waters, reducing local euthrophication and keeping degrading phenomena like hypoxia and anthropogenic pollution under control. Two Sicilian brackish-marine ponds (Ganzirri and Faro) present us with the opportunity to gain data on the effect of bivalve filtration on the amount of particulate organic matter in the field. The cultivation of bivalves has been carried out in both of the ponds since the early 1990s but stopped in Ganzirri in 1995. We tested whether the cessation of bivalve cultivation influenced features of organic matter available to suspension feeders (total suspended matter, its inorganic and organic fractions, chlorophyll a, carbohydrates, proteins and lipids). Since the bivalve cultivation was stopped in Ganzirri in 1995, chlorophyll a sharply and significantly increased compared to Faro, where, in contrast, they remained the same as in previous decades. Recent data shows that organic matter was significantly higher in Ganzirri than Faro and that differences were maintained throughout the study period. Using clearance rate data from the literature, we determined that bivalves can filter the available volume in Ganzirri by about 540 times and in Faro by 650 times per year. Thus bivalve farmed biomass (about 300 tonnes per year of fresh biomass) can exert a high filtration pressure to both (i) control the phytoplankton biomass and trophic dynamics in ponds, and (ii) reduce a possible role of natural-with-sea exchange and polluted waters coming from the hinterlands.

Removal of organic matter and nitrogen in an horizontal subsurface flow (HSSF) constructed wetland under transient loads

A monitoring campaign in a horizontal subsurface flow constructed wetland under the influence of transient loads of flow-rate, organic matter, nitrogen and suspended solids showed an irregular removal of COD and TSS and lower both removal efficiencies and mass removal rates than the ones observed in other studies for similar operating conditions. This circumstance is associated to the presence of large amount of particulate organic matter from non-point sources. The mass removal rate of ammonia increased 39% as both the water and soil temperatures increased from weeks 1-8 to weeks 9-14. A good correlation between mass load and mass removal rate was observed for all measured parameters, which attests a satisfactory response of the bed under to transient loads.