Amount Of Fluorescent Material Research Articles

1024 蛍光剤混入法による管内流計測用光学センサ

It seems to be important and efficient to monitor the flow inside a pipe, in order to analyze the flow condition and make higher controlled air-conditioners. Mixing the fluorescent material into the refrigerant has been used as the leak tester. We proposed an optical sensor system to monitor the flow condition by using the amount of scattered light and the fluorescent light from the fluorescent material changed by the flow condition. Since the scattered light is strongly influenced by the change of shape at the boundary between the liquid refrigerant and the vapor and the fluorescent light by the amount of fluorescent material, the change of both amounts of light inform us the change of flow condition. The design methodology of optical monitoring sensor was described by the experimental results

The role of calcium ions in the synthesis and transport of noradrenaline carrier vesicles in guinea-pig sympathetic neurons in vitro.

Guinea-pig inferior mesenteric ganglia (IMG)/hypogastric nerve preparations were incubated in tissue culture media containing ethylene glycol-bis-(beta-aminoethyl ether)N,N'tetra-acetic acid (EGTA) or colchicine and examined either by fluorescence microscopy or by transmission electron microscopy. Two millimolar EGTA inhibited the accumulation of noradrenaline (NA) fluophore proximal to a crush, but did not produce an increase in the fluorescent intensity of, or the number of dense-cored vesicles (DCVs) within the neuronal perikarya. Calcium ions, but not magnesium ions, were able to block this effect of EGTA. Preparations incubated in the presence of colchicine (2.5 microgram/ml) showed a reduction in the amount of fluorescent material accumulating proximal to a crush, but an increase in both the fluorescent intensity and the number of DCVs within the neuronal perikarya. The suggestion that calcium ions are required for the synthesis of some part of the NA-containing vesicle rather than for their loading onto the axoplasmic transport mechanism is discussed.

The ontogenesis of monoaminergic nerve fibres in the hypophysis of Rana temporaria with special reference to the pars distalis.

In Rana temporaria tadpoles, fluorescent fibres appear in the prospective eminentia mediana, the pars intermedia and the pars distalis at Gosner's stage 25. During prometamorphosis the amount of fluorescent material increases around the developing primary capillary plexus in the eminentia mediana. In the pars intermedia the fibres form a dense fluorescent network but in the pars distalis the fibres are few and delicate. At stages 42-43, the onset of climax, the pars distalis fibres disappear. The possible functional significance of the pars distalis fibres is discussed. The background adaptation ability appears at stages 28-29, while the fluorescent pars intermedia innervation is observable at stage 25.

Quantitative determination of griseofulvin and griseofulvin-4'-alcohol in plasma by fluorimetry on thin layer chromatograms.

Abstract A method for quantitatively determining griseofulvin and griseofulvin-4′-alcohol in plasma was developed employing measurement of fluorescence directly on thin layer chromatograms. Chromatograms of plasma extracts were mechanically scanned with a modified Photovolt TLC Densitometer. The areas under the peaks produced by the scan were found to be linear with the amount of fluorescent material on the spots within a useful range. The errors incurred in the assay were 3.2 for the determination of griseofulvin and 4.7 for griseofulvin-4′-alcohol. The results of the TLC fluorimetric assay and the spectrophotofluorometric assay on plasma samples following oral doses of griseofulvin to rabbtis were in good agreement.

XLIV.On the variation of the E.M.F. of a photoactive cell, containing a fluorescent electrolyte, when the amount of fluorescent material in solution is varied

XLIV.<i>On the variation of the E.M.F. of a photoactive cell, containing a fluorescent electrolyte, when the amount of fluorescent material in solution is varied</i>