Amount Of Citric Acid Research Articles

Optimization of citric acid production by Aspergillus niger using two downgraded Algerian date varieties.

In the present work, the GHARS and the MECH DEGLA downgraded date varieties were used in a fermentation medium in order to produce citric acid by the Aspergillus niger. The biochemical characteristics of the dates were investigated, along with the chemical and physical characteristics of the solutions of both samples. The analyzed parameters included the moisture and sugar content, the ash residual, the pH values, and the electrical conductivity. The effect of the following fermentation parameters was studied: initial pH, temperature, incubation period, and methanol. For the GHARS and MECH DEGLA date varieties respectively, the ash residual measured at 1.90% and 2.47%. For each date variety, the moisture and total sugars were measured at 11.59% and 85%, for the GHARS, and 12.82% and 80.47% for the MECH DEGLA. Citric acid production using either of the two varieties of dates showed a high yield in a short time.The obtained results showed that the highest production of citric acid by both medium of dates was achieved at the initial pH value of 3.0, temperature 30 °C, and an incubation period of 8 days. Also, the maximum amount of citric acid was produced when both mediums contained 4% of methanol. Both varieties of dates showed a good yield for the citric acid and can be used as a culture medium since they are economic and ensure good growth for the Aspergillus niger.

Oil palm trunk powder blended with citric acid directly used as wood adhesive by one step liquefaction reaction

The use of lignocellulosic raw materials to synthesize bio-adhesives through liquefaction is growing. To add value to oil palm trunk agricultural waste, this study aimed to investigate the ability to bond rubberwood veneer with powdered oil palm trunk, with or without citric acid by one-step liquefaction during hot pressing. The results revealed that the oil palm trunk powder acts as a great binder of rubberwood veneer when compressed at a medium temperature (180 °C) for 5 min. The dry shear strength was not significantly affected by the amount of citric acid when compared to control specimens bonded with only oil palm trunk powder. The acidity of oil palm powder increased with the citric acid dose. The citric acid functions as a cross-linking agent and catalyst improving water resistance, especially in acidic conditions by generating furan products that were confirmed with FTIR. It should be noted that a high dose of citric could degrade the carbohydrate polymers and reduce the lifetime of the wood product.

The Effects of Pineapple Extract Toothpaste (Ananas comosus L.Merr) on Saliva Flow Rate

Women in the menopause period experience biological changes, decreased production of the hormones estrogen and progesterone resulting in xerostomia (dry mouth). Dry mouth treatment can be carried out using mechanical and chemical stimuli. Citric acid stimulation is one way to increase the flow rate of saliva. Pineapple contains a high amount of citric acid. The purpose of this study is to determine the effect of toothpaste with pineapple extract, which increasing salivary flow rate in women in menopause period at the Integrated Health Center (Posyandu) for elderly in Baso Health Center. This type of experimental research using pretest-posttest control group design and the sample selection was taken by simple random sampling with a lottery technique. The number of samples was 32 people who were divided into two groups, namely the treatment group and the control group. Saliva was collected before and after treatment into the saliva pot for 5 minutes using the spitting method. Data analysis using paired T test and unpaired T test. The results showed that there was an increase in salivary flow rate with the use of pineapple extract toothpaste 50% of 1.651 ml/minute and with fluoride toothpaste. 1,170 ml/minute. There is an effect of brushing teeth with pineapple extract toothpaste on the salivary flow rate with a significance value of p = 0.000 <0.05. Pineapple extract toothpaste can be used as an alternative treatment for xerostomia for women in menopause period.

Effect of citric acid on the morpho-structural and magnetic properties of ultrasmall iron oxide nanoparticles

The structural and magnetic properties of iron oxide nanoparticles obtained by the precipitation of iron ions in the presence of different amounts of citric acid were studied. The citric acid acts as a capping agent and provides control of the particle size to be obtained. The size dependence of the magnetic properties was investigated by comparing the results for ~2, 4, 6, and 8 nm particles. With decreasing the particle size, a reduction of the saturation magnetization (from ~84 to 33 Am2/kg) and an increase of coercivity (from ~32 to ~100 mT) were observed, due to the increasing role of the disordered surface. The behaviour of the zero-field cooled/field cooled magnetization curves indicates a superspin-glass freezing for 2 nm and 4 nm particle samples. Memory experiments below the freezing temperature on the 2 nm particle sample provide further evidence of a glassy magnetic state. The observation of exchange bias, due to core/shell interface exchange coupling, for 2 nm and 4 nm particle samples provides evidence of a surface spin-glass type freezing, which contributes to the observed glassy properties.

Utilization of whey for formulation of whey jamun juice ice pops with antidiabetic potential

AbstractWhey utilization has been a subject of much research, as it has a very high biological oxygen demand (BOD) and must be treated prior to disposal. Several strategies, such as isolation of whey proteins, bioconversions of lactose to ethanol, and methane production, have aimed at reducing the organic load for reducing the BOD levels. In order to enhance the utilization of whey, a novel approach for formulating a functional food was employed. Whey was blended with jamun juice to prepare whey jamun ice pops (WJP) by the use of freezing technology (−20°C) with the addition of small amounts of citric acid as preservative as well as for enhancing taste and nonnutritive sugar (sucralose). The formulation containing 59.7 ml of jamun pulp juice, 40.11 ml of whey, 0.06 g of citric acid, and 0.13 g of sucralose, which ranked best among all combinations, was selected. The formulation was analyzed for physicochemical parameters, total polyphenols, total flavonoids, and sensory acceptability. WJP was found to have 78% DPPH (2,2‐diphenyl‐1‐picrylhydrazyl) inhibition (antioxidant activity), 7.89 mg GAE/ml of total polyphenols, 4.58 QE mg/ml of total flavonoids, 0.7% (v/v expressed as citric acid) titrable acidity, pH 4.3, TSS 12 °Brix, 14.3 mg/100 ml vitamin C, and 6% reducing sugar. In the present study, in vitro antidiabetic activity showed inhibition of α‐amylase activity by 60%, and in vivo activity tested in albino rats revealed that the final product helped in regulating the blood glucose in the body.

Preparation of Amaranthus caudatus L. and Punica granatum Composite Beverage and Its Resisting Exercise Fatigue Effect

The composite beverage was developed with Punica granatum fruit and polysaccharide of Amaranthus caudatus L. as main raw materials and observed its anti-fatigue activity. Sensory evaluation score was used as the index. The single factor experiments and response surface analysis method were used to optimize the formula of composite beverage, and investigateits effect of exhaustive swimming time and related biochemical indexes after animal exercise. The results showed that the best formula of the compound beverage was as follows: The volume ratio of 10% Prunus syringae polysaccharide liquid to 20% Punica granatum juice was 2:5, the amount of sucrose was 4.5%, the amount of citric acid was 0.4%, the amount of sodium carboxymethyl cellulose was 0.06%, and the sensory score was 89.8. Compared with the blank control group, the animal groups of composite beverage at middle and high dose could significantly prolong the exhaustive swimming time (P<0.01), obviously decreased BLA (P<0.01) and BUN (P<0.01) contents after exercise. Therefore, the composite beverage of Amaranthus caudatus L. and Punica granatum had better anti-fatigue function.

Crystal phase, morphology evolution and luminescence properties of Eu3+ -doped BiPO4 phosphor prepared using the hydrothermal method.

A series of Bi1-x Eux PO4 phosphors was successfully prepared using an easy citric acid- assisted hydrothermal method. The effects of synthesis temperature, amount of citric acid, and Eu3+ concentration on the crystal phase, morphology, and luminescence properties were investigated. X-ray diffraction results revealed that BiPO4 :Eu3+ formed a hexagonal phase at 80°C, although it transformed to a monoclinic phase when the hydrothermal temperature was increased to 160°C. Moreover, it was found that a small amount of Eu3+ doping and the addition of citric acid introduced no secondary or related phases. The morphologies of the samples varied from nanoparticles to spherical microparticles to polyhedrons after the synthesis temperature was adjusted. Particle size was controlled by adding different amounts of citric acid. Under 394 nm excitation, the Bi0.95 Eu0.05 PO4 phosphor prepared at 160°C with 0.3 g of citric acid had excellent luminescence properties. A possible crystal growth mechanism during this reaction is proposed.

Synthesis and characterization of potassium silicate fertilizer using fly ashes of bituminous and sub-bituminous and lignite coal

Present study focuses on coal fly ash, a waste acquired from coal power plants for the synthesis of potassium based fertilizer by means of coal fly ash and lignite with simple blending of KOH and adding small amount of citric acid. Lignite and two types of fly ash bituminous and sub-bituminous were utilized for the formulation of fertilizer. X-ray diffraction (XRD) revealed the occurrence quartz and oxides of potassium in all fly ashes. XRD pattern also exhibited the orthoclase, mullite and zeolite as major compounds in fertilizer which were made from sub-bituminous ash. K2O, P2O5 and N were determined as essential compounds in sub-bituminous through using wet analysis. However, major presence of humic acid and organic matter were observed in lignite through wet analysis. Fourier transform infrared (FTIR) spectroscopy also confirmed the occurrence of humic acid in the form of carboxylic group in lignite and bituminous. Surface Mean Diameter measured by sieve analysis showed that particle size of sub-bituminous ash was smaller than others that most appropriate for synthesis of potassium silicate fertilizer.

Fruit Characteristics of Different Varieties of Cornelian Cherry (Cornus mas L.) Cultivated in the Czech Republic

The use of the fruit of the Cornus mas L. in the food industry has a long tradition in the Czech Republic. Although these fruits currently belong to marginal species, there is an increasing interest in cultivating this crop. The main goal of the present study was to determine pomological properties of 6 varieties and 4 ecotypes of Cornus mas L. fruit grown in the Czech Republic and to find out whether it is possible to differentiate these varieties and ecotypes from one another by means of analysis of variance of the obtained data. To exclude the impact of climatic conditions, fruits harvested in three seasons (2010–2012) were analysed. The results showed that the chemical composition of Cornus mas L. varieties or ecotypes originating in the Czech Republic (‘Ruzynsky’, ‘Tisnovsky’, ‘Olomoucky’), Ukraine (‘Elegantni’, ‘Vydubecky’, ‘Vysegrodsky’, ‘Lukjanovsky’), Austria (‘Fruchtal’, ‘Jolico’) and Slovakia (‘Sokolnicky’) is significantly different. Varieties or ecotypes of Cornus mas L. originating in the Czech Republic contained high amount of ascorbic acid (avg. 315 mg kg−1 f.w.). Varieties of Cornus mas originating in Austria were rich in polyphenols (avg. 1728 mg kg−1 f.w.). Slovakian ecotype of Cornus mas ‘Sokolnicky’ contained the highest amount of citric acid (avg. 0.53 g kg−1 f.w.). Ukrainian varieties were characterized by a higher content of trace elements (avg. 2.41 mg kg−1 Cu and 3.37 mg kg−1 Zn f.w.).

Wet extrusion molding of wood powder with hydroxy-propylmethyl cellulose and with citric acid as a crosslinking agent

To mitigate global warming and the serious problems incurred by the disposal of petroleum-based plastics, it is important to develop derivatives of biomass materials that can be used as substitutes. To overcome the lack of thermoplasticity of wood, a wet extrusion molding process for wood powder using a cellulose derivative, hydroxypropylmethyl cellulose (HPMC), had been developed. However, this material quickly reabsorbed water, swelled, and disintegrated in liquid. In the present study, a natural organic acid, citric acid, was added and kneaded together with the wood powder, the HPMC, and water. The resultant clay-like material was extruded into a tube-shaped material. The tube was air-dried and heated at 180 °C for 5 min to 30 min to allow crosslinking. By heating 1% citric acid for 30 min, the material avoided disintegrating in water for 60 min. The addition of 3% citric acid with 30 min crosslinking gave the material water resistance in water for 12 h. The degradability in the water was found to be controllable by changing the amount of citric acid and the heating time. This is a novel result because wood can be molded into a practical three-dimensional (3D) biomass composite material using this technology with natural substances without relying on petroleum-based plastics.

Colonization and bioweathering of monazite by Aspergillus niger: solubilization and precipitation of rare earth elements.

Geoactive fungi play a significant role in bioweathering of rock and mineral substrates. Monazite is a phosphate mineral containing the rare earth elements (REE) cerium, lanthanum and neodymium. Little is known about geomicrobial transformations of REE-bearing minerals which are also relevant to REE biorecovery from terrestrial and extra-terrestrial reserves. The geoactive soil fungus Aspergillus niger colonized monazite in solid and liquid growth media without any apparent growth inhibition. In a glucose-minerals salts medium, monazite enhanced growth and mycelium extensively covered rock particle surfaces, probably due to the provision of phosphate and essential trace metals. Teeth-like and pagoda-like etching patterns indicated monazite dissolution, with extensive precipitation of secondary oxalate minerals. Biomechanical forces ensued causing aggressive bioweathering effects by tunnelling, penetration and splitting of the ore particles. High amounts of oxalic acid (~46 mM) and moderate amounts of citric acid (~5 mM) were produced in liquid media containing 2% (wt./vol.) monazite, and REE and phosphate were released. Correlation analysis suggested that citric acid was more effective than oxalic acid in REE mobilization, although the higher concentration of oxalic acid also implied complexant activity, as well as the prime role in REE-oxalate precipitation.

Mosaic structure ZnO formed by secondary crystallization with enhanced photocatalytic performance

Zinc acetate is used as a raw material to synthesize the desired ZnO in hot solvent by controlling the amount of citric acid (CA) added. Notably, the amount of CA added has a significant relationship with the control of the morphology of ZnO. Spherical ZnO wrapped in nanosheets is synthesized through the secondary crystallization of Zn2+. The optical properties of the ZnO sample are tested through the degradation of organic pollutants. Notably, the photocatalytic properties of ZnO vary with the different amounts of CA added. Exposure of the active crystal face increases the photocatalytic activity of ZnO. In addition, the number of defects on the surface of the ZnO sample increases because of its large specific surface area, thus changing the bandgap of ZnO. Therefore, the resulting sample can respond under visible light.

Nanomaterial Antibacterial Technology in the Design of Antibacterial Fabrics for Sports Clothing

In recent years, nanomaterials have been widely used in sports equipment, and their application in sportswear has also attracted wide attention. This research mainly discusses the application of nanomaterial antibacterial technology in the design of antibacterial fabrics for sports apparel. The method of adding nano‐TiO2 to the antibacterial textiles varies with the type of fiber or fabric, and the finishing method also changes accordingly. For chemical fibers, the method of blending spinning is mainly used. By adding nano‐antibacterial particles in the spinning solution, the polymer is ejected from the spinneret to make antibacterial fibers. This processing method is characterized by good antibacterial durability. The preparation of antimite finishing agent is mainly the dispersion of nanopowder. The selection of the best dispersant is mainly determined by measuring the change of the volume sedimentation rate of TiO2 after 5 days under different dosage and pH value of each dispersant. Then, the particle size of nano‐TiO2 and Ag in different dispersing conditions was tested by TEM, and finally, the best dispersing agent and dispersing process were determined. The optimum of nano‐antimite and antibacterial finishing agent is as follows: the dispersing agent is TW‐80, the amount of anhydrous ethanol in dispersing medium is 5 times that of powder, the amount of dispersing agent is 1.5 times that of powder, the pH value is 7, and the stirring time is not less than 30 min. In the preparation of nano‐antimite and antibacterial finishing agent, nanomaterials were used. The weight of nano‐TiO2 powder was 20 grams and that of Ag was 20 grams. The dispersion medium (absolute ethanol) was 100 mL of dispersant. The amount of TW‐80 was 30 g, the amount of citric acid was 60 g, the emulsion was very stable, and there was only a small amount of precipitation after being static for 5 days. Considering the characteristics of silk knitted fabric, this experiment uses the exhaustion method in the finishing process to finish the silk knitted fabric. After 50 times of washing, the antibacterial rate of Staphylococcus aureus and Escherichia coli reached 95.13% and 87.44%, respectively. This study provides guidance for the application of nanomaterials in the antibacterial field of sports clothing.

Phenolic components, ascorbic acid and organic acids profiling of six guava varieties grown under subtropical region of Lucknow, Uttar Pradesh

Guava (Psidium guajava L.) is a well known source of ascorbic acid, phenolic compounds and organic acids. Both ascorbic acid and phenolic components are powerful antioxidants and organic acids impart flavour in fruits. Gallic acid, chlorogenic acid, catechin, epicatechin, caffeic acid, ellagic acid and p-coumaric acid as phenolic components and ascorbic acid, oxalic acid, citric acid, tartaric acid and malic acid as organic acids were identified in fruits of six guava varieties at edible ripe stage using HPLC-PDA. Maximum amount of ascorbic acid (234.80 mg 100g-1) was recorded in Dhawal followed by Allahabad Safeda (198.80 mg 100g-1). Among the phenolic compounds, gallic acid, chlorogenic acid and catechin were the predominant ones. Maximum amounts of gallic acid (8.00 mg 100g-1), chlorogenic acid (9.00 mg 100g-1) and epicatechin (6.00 mg 100g-1) were detected in Shweta. Lalit had maximum amounts of catechin, ellagic acid, caffeic acid and p-coumaric acid (6.00, 5.93, 2.46 and 0.90 mg 100g-1, respectively). Citric acid was the major organic acid noticed in guava fruits distantly followed by tartaric acid. Lalima contained maximum amounts of citric acid (460.00 mg 100g-1) and malic acid (2.50 mg 100g-1). The genotype Sardar possessed maximum amount of tartaric acid (71.00 mg 100g-1) and Dhawal that of oxalic acid (18.50 mg 100g-1). In terms of ascorbic acid, Dhawal and Allahabad safeda were the best varieties for consumption, while Shweta and Lalit were the best varieties to consume having anticancer antioxidant phenolic components.

Citric Acid Water as an Alternative to Water Restriction for High-Yield Mouse Behavior.

Powerful neural measurement and perturbation tools have positioned mice as an ideal species for probing the neural circuit mechanisms of cognition. Crucial to this success is the ability to motivate animals to perform specific behaviors. One successful strategy is to restrict their water intake, rewarding them with water during a behavioral task. However, water restriction requires rigorous monitoring of animals’ health and hydration status and can be challenging for some mice. We present an alternative that allows mice more control over their water intake: free home-cage access to water, made slightly sour by a small amount of citric acid (CA). In a previous study, rats with free access to CA water readily performed a behavioral task for water rewards, although completing fewer trials than under water restriction (Reinagel, 2018). We here extend this approach to mice and confirm its robustness across multiple laboratories. Mice reduced their intake of CA water while maintaining healthy weights. Continuous home-cage access to CA water only subtly impacted their willingness to perform a decision-making task, in which they were rewarded with sweetened water. When free CA water was used instead of water restriction only on weekends, learning and decision-making behavior were unaffected. CA water is thus a promising alternative to water restriction, allowing animals more control over their water intake without interfering with behavioral performance.

Study of the Composition of a Precipitate Formed from Solutions for the Synthesis of Cathodic Materials Containing Manganese and Citric Acid

Cathode materials of lithium-ion batteries can be efficiently and quickly obtained by solution combustion synthesis (SCS) from nitrate solutions of d-elements and lithium in the presence of organic complexing agents such as glycine and citric acid. However, during the storage of weakly acidic nitric acid solutions of manganese, nickel, and cobalt, after the addition of glycine and citric acid, a pink precipitate forms, which violates the stoichiometry of the initial solution. Thermal and X-ray structural analysis, as well as chemical analysis to determine the carbon content, show that the precipitate is manganese citrate of the composition Mn(HC6H5O7)H2O. It is found that the composition of manganese citrate does not depend on the ratio of the amount of citric acid and metal nitrates in the working solution.

FORMULATION AND DEVELOPMENT OF BILAYER FLOATING TABLET OF AMOXICILLIN

Bilayer floating tablet of amoxicillin was formulated successfully and evaluated under suitable parameters. All the batches of tablet produced were found to exhibit short floating lag times. The tablet of batch F2 exhibited a longer floating lag time of 23 minutes. Relationship between the dependent and independent variables was further elucidated using contour and response surface plots. Dissolution profiles that the tablets of batch F3, F7, and F12 exhibits initial burst phase during the first hour of dissolution. The burst phase was followed by a limited drug release for the rest of the period. Also it was observed during the dissolution studies that tablets of all three batches eroded quickly with increased effervescence. Time required for 50 % drug to get released (T50%) and %CR10hrs were found to be in the range of 0.7 to 8.6 hours and 57.35 ± 3.89 to 99.93 ± 0.07 respectively, value of “Prob &gt; F” less than 0. 05. Response surface plots and Contour plot indicated that at a fixed level of B (35 mg) and low level of A (amount of HPMC), % CR10hrs increases from 68.11 to 90.00 % and T50% decrease from 6.86 to 1.66 as the amount of citric acid (C) increases from 0 to 10 mg. Stability study was performed for optimized formulation and it was found that formulation was stable for 6 week at 25 °C/ 60% RH. KEYWORDS: Bilayer floating tablet, amoxicillin, Evaluated, Multi-layered tablet, Stability

MCM-41 grafted with citric acid: The role of carboxylic groups in enhancing the synthesis of xanthenes and removal of heavy metal ions

Order mesoporous MCM-41 was synthesized via a sol-gel method and then grafted by adding the appropriate amount of citric acid (CA). The structure and morphology of pure and grafted mesoporous MCM-41 were investigated by XRD, BET, TEM, and FT-IR measurements. TEM measurements confirm that MCM-41 particles display an ordered structure with uniform mesoporous arranges into a hexagonal, honeycomb-like lattice and still preserved their mesoporous structure after grafting with different weight contents of CA. The pore sizes and volume of the CA/MCM-41 samples were found to vary markedly within CA contents. The surface acidity and the type of acid sites (Brønsted and Lewis) were determined using the potentiometric titration method and the FTIR of pyridine adsorption. In this work, it was found that the textural, acidic properties and the activities of catalysts could be improved by citric acid treatment The results illustrated that the surface acidity and acidic strength of CA/MCM-41 gradually increased with increasing the CA amount up to 50 wt.%. Also, the prepared catalysts showed both Brönsted and Lewis acid sites and the number of Brönsted acid sites increased largely compared with Lewis acid sites with increasing the CA content which indicates the role of carboxylic groups in enhancing the Brönsted acid sites. The catalytic performance of the samples was tested by the synthesis of 14-Phenyl-14H-dibenzo [a, j] xanthene (xanthene). In this reaction, the sample with 50 wt.% of CA displayed the highest acidity and catalytic activity. The adsorption activity was examined for removing Cd2+ and Pb2+ from aqueous solution with varying parameters such as pH, concentration, temperature and time. The equilibrium adsorption data obtained fitted with the Langmuir model and the adsorption kinetics favored the pseudo 2nd order model. Also, the adsorption was spontaneous and exothermic.

Apophysomyces jiangsuensis sp. nov., a Salt Tolerant and Phosphate-Solubilizing Fungus from the Tidelands of Jiangsu Province of China.

A newly isolated phosphate-solubilizing fungus from the topsoil of Spartina alterniflora habitats in Yancheng coastal salt marsh was cultivated. Scanning electron microscopy observation revealed that the sporangia are nearly spherical, peach-shaped, and the spores formed on the top of sporangia. The spores are ellipsoidal with raised white nubbins on the surface. Based on a polyphasic study and the genetic distance analysis referring to the sequence analysis of ITS (ITS1 + 5.8S + ITS2) and 28S rDNA (D1/D2 domains) genes, the novel species belongs to the genus Apophysomyces and is named as A. jiangsuensis. The optimum growth temperature and salinity of the new species were 28 °C and 1.15% NaCl, respectively. A study of its phosphate-solubilizing ability revealed that the fungus had an obvious decomposition effect on lecithin, Ca3(PO4)2, and AlPO3, respectively. The pH of the fermented liquid progressively decreased from 6.85 to 2.27 after 7 days of incubation, indicating that the low molecular weight organic acids excreted into the culture liquor were oxalic, succinic, and malic acids and a trace amount of citric acid. Among these, oxalic acid was the major organic acid, and its amount reached 652.5 mg/L. These results indicated that the main mechanism underlying the dissolved phosphorus was related to the secretion of large amounts of organic acids.

Sustainable production of value-added chemicals and fuels by using a citric acid-modified carbon nitride optical semiconductor

Citric acid-modified graphite-like carbon nitride materials (GCN-zCA) were synthetized by thermal co-polymerization of dicyandiamide with different amounts of citric acid (z = between 5 and 25 mg). The resulting materials presented surface porosity, defective polymeric structure, and enhanced visible light absorption in the 450−700 nm range, attributed to the existence of mid-gap states and n-π* electronic transitions. All the modified catalysts presented high selectivity (>99 %) towards the conversion of p-anisyl alcohol into p-anisaldehyde under visible-LED irradiation, the best performing photocatalyst (GCN-20CA) reaching 63 % yield (contrasting with 22 % obtained with bulk GCN) after 240 min reaction. GCN-20CA was also applied for hydrogen generation from water splitting. The modified material practically duplicated the hydrogen production when compared to bulk GCN (75 and 44 μmol H2 evolved in three hours, respectively), by using platinum nanoparticles as co-catalyst and EDTA as sacrificial electron donor. Moreover, p-anisyl alcohol was successfully used as sacrificial agent for water splitting, with simultaneous production of p-anisaldehyde and H2. Reusability tests showed that GCN-20CA remained stable in a series of consecutive runs both for p-anisaldehyde synthesis and hydrogen production.