Amount Of Cell Adhesion Research Articles

Preparation of Cobalt–Chromium Alloy Planting Materials and Its Application in the Treatment of Gum Restoration

The preparation of cobalt–chromium alloy materials by different processes was studied and used to repair the full crown of experimental dogs. First, the casting method combined with 3D printing to obtain a cobalt–chromium alloy test piece, and the mechanical and microscopic properties of the test piece were analyzed during the preparation process; And then, selective laser melting (SLM) was used to perform single-channel, single-layer, and multi-layer scanning on the specimens obtained based on this technology during the preparation process. Two kinds of samples were selected from the cobalt–chromium test pieces obtained by the casting method, which were CS01 and CS02. And then selected one kind of sample from the cobalt–chromium test piece obtained by the SLM method, which was SLM01. Firstly, the roughness and contact angle of the planting material were compared, and the saliva-streptococcus mutant cell culture solution was configured to compare the amount of cell adhesion on the surface of different planting materials. Finally, the planting materials were used to repair the dog's full crown and establish animal models. The expressions of Bcl-2, Bax, Ki67 and p53 genes in gingival cells before and after canine crown wearing were compared in gingival tissues. The test results show that the mechanical properties and microstructure of the cobalt–chromium alloy material obtained based on the casting method meet the requirements. Based on the cobalt–chromium test piece obtained by the SLM method, the process parameters were optimized so that the corresponding density of the test piece reached 98.3%; Comparing roughness and surface contact angle, there was no statistical difference between different planting materials (P > 0.05 ). At the same time, the number of Streptococcus mutant adhered to the surface of different specimens was not statistically significant (P > 0.05 ); In the expression of gingival tissue, the implant material was obtained based on the SLM01 test piece, which promoted the decrease of Ki67 gene expression (P < 0.05 ), and also promoted the proliferation of other gingival cell genes (P < 0.05 ).

Nanolayer Film on Poly(Styrene/Ethylene Glycol Dimethacrylate) High Internal Phase Emulsion Porous Polymer Surface as a Scaffold for Tissue Engineering Application

Highly open, porous polymer or poly(high internal phase emulsion) (polyHIPE) obtained from polymerized high internal phase emulsions (HIPE) have recently attracted much attention and increasing interest in tissue engineering applications because of their excellent properties. This research is aimed at preparing and developing a process for producing polyHIPE for use as a scaffold in tissue engineering applications. Poly(styrene/ethylene glycol dimethacrylate) (poly(S/EGDMA)) loaded with hydroxyapatite was used to prepare the polyHIPE. Further improvement on hydrophilicity and biological response to tissue fluids of the polyHIPE porous polymer was carried out using a nanolayer coating via the layer-by-layer polyelectrolyte multilayer (PEM) technique. Three types of chemicals were used for coating on the surface of the polyHIPE porous polymer such as poly(sodium 4-styrene sulfonate) (PSS), gelatin (GEL), and alginic acid (ALG). The change in surface properties of the modified poly(S/EGDMA)HIPE was characterized by UV-visible spectroscopy and contact angle measurement. Further assessments consisted of cytotoxicity testing, cell attachment, and proliferation of L929, fibroblast-like cells that were seeded on the surface of the polyHIPE porous polymer. A three-dimensional structure poly(S/EGDMA)HIPE porous polymer with high porosity was successfully prepared. Moreover, it was found that surface modification encouraged poly(S/EGDMA)HIPE with a hydrophilic nanolayer, as observed by the decrease in contact angle degree and cell adhesion of L929 fibroblast-like cells, indicating that the poly(S/EGDMA)HIPE porous polymer was effectively improved by using the layer-by-layer technique. It was revealed by MTT assay that the poly(S/EGDMA)HIPE porous polymer coated with a nanolayer of a polyelectrolyte multilayer led to an enhancement in the amount of cell adhesion and proliferation on the modified poly(S/EGDMA)HIPE porous polymer. Additionally, the most effective polyelectrolyte solution for improving cell adhesion of the poly(S/EGDMA)HIPE was PSS.

Correlation of proliferation, morphology and biological responses of fibroblasts on LDPE with different surface wettability

In order to find a correlation between cell adhesion, growth and biological response with different wettability, NIH/3T3 fibroblast cells were cultured on plasma-treated low-density polyethylene (LDPE) film generated with radio frequency. Different surface wettabilities (water contact angle 90–40°) were created by varying the duration of plasma treatment between 0 and 15 s, respectively. Growth and proliferation rate of cells on LDPE surfaces was evaluated by MTT assay, and cell morphology, by means of spreading and adhesion, was characterized by scanning electron microscopy (SEM). The expression of particular genes in cells contacted on films with different wettability was analyzed by RT-PCR. Using the MTT assay, we confirmed that the amount of cell adhesion was higher on surface of film with a water contact angle of 60° than with other water contact angle. Also, the proliferation rate of cells was highest with a water contact angle of 60°. It was confirmed by SEM that the morphology of cells adhered with a water contact angle of 50–60° was more flattened and activated than on other surfaces. Furthermore, c-fos mRNA in cells showed maximum expression on the film with contact angle range of 50–60° and c-myc mRNA expressed highly on the film with a contact angle of 50°. Finally, p53 gene expression increased as wettability increase. These results indicate that a water contact angle of the polymer surfaces of 50–60° was suitable for cell adhesion and growth, as well as biological responses, and the surface properties play an important role for the morphology of adhesion, growth and differentiation of cells.

Involvement of the muscarinic acetylcholine receptor in inhibition of cell migration

Activation of G protein-coupled receptors is known to stimulate cell migration, but receptor-mediated signals inhibiting cell migration have not been identified. We investigated the ability of transfected human M 3 muscarinic acetylcholine receptors (mAChR) to regulate the migration of Chinese hamster ovary (CHO) cells. Single cells migrated on colloidal gold applied to fibronectin-coated plates, and videomicroscopy was used to measure cell spreading and migration. Activation of M 3 mAChR with the agonist carbachol was found to inhibit cell migration, whereas direct activation of protein kinase C (PKC) with PMA was found to stimulate migration. The amount of cell adhesion and spreading was found to be equivalent for carbachol- and PMA-treated cells. Selective inactivation of conventional PKC isoforms with Go6976 (C 24H 18N 4O) abolished the PMA-mediated increase in cell migration. In contrast, the mAChR-mediated decrease in migration was not altered by Go6976, but was abolished when both novel and conventional PKC isoforms were inactivated by calphostin C or chelerythrine. These findings suggest involvement of conventional PKC isoforms in the stimulation of migration and of novel PKC isoforms in the inhibition of migration. Carbachol- but not PMA-treated cells exhibited an elongated morphology reminiscent of migrating cells that cannot detach their trailing edges from the substratum. Similarly, carbachol-treated cells detached less readily from fibronectin than control or PMA-treated cells when integrin activity was diminished by the chelation of Ca 2+ and Mg 2+. Finally, the carbachol-induced diminution of cell detachment was preserved after inhibition of the conventional PKC isoforms with Go6976, but was abrogated by treatment with either calphostin C or chelerythrine. These findings suggest that mAChR activation diminishes the ability of cells to detach from the substratum, resulting in diminished migration. This is in contrast to the direct activation of PKC with PMA, which stimulates migration.