Preparation of Cobalt–Chromium Alloy Planting Materials and Its Application in the Treatment of Gum Restoration

Abstract

The preparation of cobalt–chromium alloy materials by different processes was studied and used to repair the full crown of experimental dogs. First, the casting method combined with 3D printing to obtain a cobalt–chromium alloy test piece, and the mechanical and microscopic properties of the test piece were analyzed during the preparation process; And then, selective laser melting (SLM) was used to perform single-channel, single-layer, and multi-layer scanning on the specimens obtained based on this technology during the preparation process. Two kinds of samples were selected from the cobalt–chromium test pieces obtained by the casting method, which were CS01 and CS02. And then selected one kind of sample from the cobalt–chromium test piece obtained by the SLM method, which was SLM01. Firstly, the roughness and contact angle of the planting material were compared, and the saliva-streptococcus mutant cell culture solution was configured to compare the amount of cell adhesion on the surface of different planting materials. Finally, the planting materials were used to repair the dog's full crown and establish animal models. The expressions of Bcl-2, Bax, Ki67 and p53 genes in gingival cells before and after canine crown wearing were compared in gingival tissues. The test results show that the mechanical properties and microstructure of the cobalt–chromium alloy material obtained based on the casting method meet the requirements. Based on the cobalt–chromium test piece obtained by the SLM method, the process parameters were optimized so that the corresponding density of the test piece reached 98.3%; Comparing roughness and surface contact angle, there was no statistical difference between different planting materials (P > 0.05 ). At the same time, the number of Streptococcus mutant adhered to the surface of different specimens was not statistically significant (P > 0.05 ); In the expression of gingival tissue, the implant material was obtained based on the SLM01 test piece, which promoted the decrease of Ki67 gene expression (P < 0.05 ), and also promoted the proliferation of other gingival cell genes (P < 0.05 ).