Effect of air bubbles on the membrane filtration of a basic dye, rhodamine B (RB), using a hydrophilic PTFE membrane filter (pore size: 0.20μm) was studied. The air bubbles were generated by vigorously mixing the aqueous solution containing 0.05% (v/v) of 1-butanol with a shaft generator of a homogenizer. RB being far smaller than the pore size of the membrane filter could not be rejected without air bubbles, but it was rejected by the membrane filter in the presence of air bubbles. The rejection ratio increased with increasing the rotation speed of the shaft generator because of the increase in the amount of air bubbles and therefore the increase in the surface area of air bubbles for the adsorption of RB. On the other hand, another basic dye, methylene blue (MB), was negligibly rejected in the same condition. Dynamic surface tension measurement of aqueous solutions containing different amounts of dye indicated that RB strongly adsorbed to the air-water interface, while MB hardly adsorbed. The results obtained in the present study strongly suggest the potential usefulness of air bubbles for the selective microfiltration of dissolved organic molecules or ions.