Ammonium Hydroxide Research Articles

Study on solubility and solvation thermodynamics for the advancement of biorelevant activities of l-isoleucine and l-serine in aqueous ammonium chloride solutions in the temperature range of 288.15–308.15 K

This study investigated the dissolution behavior of l-isoleucine and l-serine in an aqueous salt solution (ammonium chloride), examining how variations in temperature and electrolyte concentration affect their solubility. We conducted careful experiments and used mathematical calculations to explore interactions at a molecular level. We observed that the structure of these amino acids and salt concentration in the aqueous medium influence their interactions, which affects dissolution. In the presence of electrolytes, l-isoleucine demonstrated a salting-out effect whereas l-serine showed a salting-in effect. This work examines the solute–solvent interactions of these solutes in aqueous ammonium chloride solutions. l-isoleucine exhibits a nonspontaneous reaction with increasing salt concentrations whereas l-serine shows spontaneous behavior. Gibbs free energy analysis revealed greater stability of l-serine. The pH and conductance measurements showed how these factors influence solution properties. This insight helps us comprehend the nature and behavior of these molecules in different situations, which could be helpful in drug formulation or protein purification in the future.

Interlayer engineering of V2O5·nH2O by conductive Ni-BTA enabling high-performance aqueous ammonium ion batteries

Aqueous ammonium ion batteries (AAIBs) are of interest due to the low molar mass, small hydration radius, abundant raw materials and high safety of the carrier ammonium-ion. Nevertheless, there are numerous constraints associated with electrode materials that are suitable for ammonium-ion storage. In this study, we design and synthesize a composite comprising of one-dimensional conductive metal-organic skeleton material (1D c-MOF) embedded between layers of hydrated vanadium pentoxide (VOH) with improved ammonium-ion storage. The central ion of the 1D c-MOF is selected to be the nickel ion, while the ligand is 1,2,4,5-benzenetetramine (BTA). The incorporation of Ni-BTA between the vanadium oxide layers results in the formation of a composite (Ni-BTA/VOH) exhibiting enhanced structural stability, augmented layer spacing and elevated conductivity. Furthermore, the dual energy storage mechanisms of VOH and CN rearrangement act in concert to yield a “1+1 > 2” effect, thereby markedly enhancing the ammonium-ion storage. The specific capacity of Ni-BTA/VOH can reach 183 mAh g−1 at 0.2 A g−1, and the retention rate can reach 52.5 % after 500 cycles at 2 A g−1. This work not only proves the potential of Ni-BTA/VOH for widespread application in the field of aqueous batteries, but also provides a new method for structural engineering of VOH with boosted ammonium-ion storage properties.

Biomass Demineralization and Pretreatment Strategies to Reduce Inhibitor Concentrations in Itaconic Acid Fermentation by Aspergillus terreus

Itaconic acid (IA) is a platform chemical, derived from non-petroleum sources, produced through the fermentation of glucose by Aspergillus terreus. However, producing IA from alternative sugar sources (e.g., lignocellulose) has been shown to be problematic, requiring post-hydrolysis mitigation to allow growth and IA production by the fungus. It is well known that the side products of lignocellulosic biomass conversion to sugars act as microbial growth inhibitors. An uncommon feature of fungal organic acid fermentations is production inhibition caused by mineral ions in biomass hydrolysate after pretreatment and enzymatic hydrolysis. To minimize mineral introduction during pretreatment and hydrolysis, we determined the sources of growth and production inhibitors at each of these steps. Biomass demineralization and four pretreatment strategies were evaluated for inhibitor introduction. Dilution assays determined the approximate degree of inhibition for each hydrolysate. An ammonium hydroxide pretreatment of demineralized wheat straw presented the lowest concentration of inhibitors and concomitant lowest inhibition: subsequent fermentations produced 35 g L−1 IA from wheat straw hydrolysate (91 g L−1 sugar) without post-hydrolysis mitigation.

Enhanced Energy Storage Capacity of TiO2 Atomic Layered Molybdenum Oxide–Sulfide Negatrode for an Aqueous Ammonium Ion Supercapacitor

Enhanced Energy Storage Capacity of TiO2 Atomic Layered Molybdenum Oxide–Sulfide Negatrode for an Aqueous Ammonium Ion Supercapacitor

Extraction of molybdenum, cobalt, and nickel from acidic leach solution of molybdenum leach residue of spent hydrodesulfurization catalyst

ABSTRACT After the extraction of molybdenum from the spent HDS catalyst, a residue containing aluminum, nickel, cobalt, and a small amount of molybdenum was obtained. The simultaneous recovery of nickel, cobalt, aluminum, and residual molybdenum from molybdenum leach residue can be achieved through hydrochloric acid leaching. In the work presented herein, the extraction of molybdenum, cobalt, and nickel from the acidic leach solution of molybdenum leach residue was investigated. Trioctyl tertiary amine can selectively extract cobalt and molybdenum from the acidic leach solution, the extraction of both cobalt and molybdenum reached more than 99% after two-stage countercurrent extraction. The combination of pure water and 25% ammonia water can be utilised for the separation and extraction of cobalt and molybdenum from the loaded cobalt-molybdenum organic phase. After the extraction of cobalt and molybdenum, a synergistic extraction system comprising decyl 4-picolinate and dinonylnaphthalene sulfonic acid can be employed for nickel extraction, achieving a nickel extraction exceeding 99.70% while maintaining aluminum extraction at only 0.32% after undergoing four-stage countercurrent extraction. The nickel stripping efficiency can reach 99.50% after four-stage countercurrent stripping, using a stripping agent of 2 mol/L H2SO4.

A Review of the Technological Aspects and Process Optimization of Bioethanol Production From Corn Stover Biomass: Pretreatment Process, Hydrolysis, Fermentation, Purification Process, and Future Perspective

ABSTRACTBioethanol, a sustainable energy solution derived from renewable biomass, has gained prominence, with corn stover emerging as a substantial biomass resource in Indonesia. Corn stover, a corn residue, is one of the top three agricultural wastes worldwide and is abundantly available. However, a significant portion of corn stover is burned in fields rather than utilized for bioethanol production, whereas it has potential as a bioethanol feedstock. As the world strives to realize sustainable and environmentally friendly energy security, bioethanol production from corn stover can be one of the solutions to be developed. Nonetheless, the current immaturity of bioethanol production technology is one of the causes of large‐scale production failure. The present paper comprehensively reviews the technological aspects and process optimization of bioethanol production using corn stover as a feedstock comprising pretreatment, hydrolysis, fermentation, and bioethanol purification processes. According to our critical review, ammonia fiber expansion (AFEX) pretreatment is the most effective conventional pretreatment, with glucose yield up to 90%. Moreover, ultrasound appears to be the most viable option for nonconventional pretreatment of corn stover for producing bioethanol. However, combining ultrasound pretreatment and dilute aqueous ammonia produced 80.6% sugar output. Furthermore, enzymatic hydrolysis emerges as the most effective saccharification, yielding up to 81.39%. Moreover, the fermentation process of corn stover with the saccharification and co‐fermentation (SScF) method and the process optimization with response surface methodology (RSM) could produce bioethanol with a concentration of up to 59.8 g/L and 92.07% ethanol yield, respectively. This review also reveals that pervaporation for the purification process is the best choice for producing bioethanol with high purity up to > 99%. In addition, this method could reduce the energy used by 6.6% lower, 24.2% lower carbon footprint, and have the lowest total capital and production costs compared to conventional molecular sieves and extractive distillation. We believe this review article can provide a reference for selecting the best bioethanol production process from corn stover for further research.

Pump-Free Pneumatic Actuator Driven by the Vapor Pressure at the Gas-Liquid Equilibrium of Aqua Ammonia.

Currently, pneumatic soft actuators are widely used due to their impressive adaptability, but they still face challenges for more extensive practical applications. One of the primary issues is the bulky and noisy air compressors required to generate air pressure. To circumvent this critical problem, this work proposes a new type of air pressure source, based on the vapor pressure at the gas-liquid equilibrium to replace conventional air pumps. Compared with the previous phase transition method, this approach gains advantages such as generating gas even at low temperatures (instead of boiling point), more controllable gas output, and higher force density (since both ammonia and water contribute to the gas pressure). This work built mathematical models to explain the mechanism of converting energy to output action force from electrical energy and found the aqua ammonia system is one of the optimal choices. Multiple prototypes were created to demonstrate the capability of this method, including a pouch actuator that pushed a load 20,555 times heavier than its dead weight. Finally, based on the soft actuator, an untethered crawling robot was implemented with onboard batteries, showing the potentially extensive applications of this methodology.

Advanced HPLC Method with Diode Array Detection Using a Phenyl-Bonded Column for Simultaneous Quantitation of Three Sunscreen Filters in a Moisturizing Sunscreen Cream for Acne-Prone Skin

This study introduces a novel, robust, and efficient method for the simultaneous quantitative determination of three sunscreen filters, namely, 4-methylbenzylidene camphor, octyl methoxycinnamate, and avobenzone, in a moisturizing sunscreen cream specifically designed for acne-prone skin. The method employs high-performance liquid chromatography with photodiode-array detection, providing a reliable separation of the analytes. Chromatographic separation was achieved using a Fortis Phenyl analytical column (150.0 × 2.1 mm, 5 μm), with isocratic elution at a flow rate of 0.4 mL/min. The mobile phase was composed of a 57/43 (v/v) mixture of acetonitrile/45 mM aqueous ammonium formate solution, ensuring sufficient resolution and peak symmetry for the target compounds. The method was validated comprehensively for critical performance parameters, including linearity, precision, accuracy, and robustness. Linearity was established across a suitable range for all three analytes, with high correlation coefficients. Precision was confirmed with intra-run and total precision coefficients of variation of ≤4.6%, while accuracy assessments yielded a percent recovery between 98.6 and 100.4, for all quality control levels. Additionally, the method was able to effectively separate the sunscreen filters from other cosmetic ingredients, such as [β-(1.3), (1.6)-D-glucan], low molecular weight (LMW) hyaluronic acid and plant extracts ensuring specificity in complex formulations. This straightforward and time efficient sample preparation process, involving methanol extraction followed by serial dilution, makes the method suitable for routine quality control in cosmetic laboratories. The method was successfully applied to the analysis of two different lots of a commercial sunscreen cream, achieving excellent recovery for all filters, ranging between 94.6% and 99.8%, thus demonstrating its reliability and applicability for the quality control of cosmetics.

Structure and Properties of Thin Films Prepared on Flexible Substrates from SnCl4-Derived Solutions

Thin transparent films of SnO2 were obtained from aqueous–alcohol solutions of SnCl4 on a flexible polyethylene terephthalate (PET) substrate by spray pyrolysis at 100 °C. The influence of the addition of aqueous ammonia to the film-forming solution on the different properties has been studied. Properties studied include surface morphology, phase composition and transparency of the formed films and the crystallization processes and band gap of the film material. It was found that the addition of aqueous ammonia causes the formation of skeletal crystals (NH4)2[SnCl6] with a perovskite structure in the film structure. The resulting films are promising for use in the technology of manufacturing flexible solar cells.

In situ synthesis of nano-CeO2 and chitosan composite

Nanosized cerium oxide (CeO2) particles were prepared by co-precipitation method using chitosan as a template, cerium (III) nitrate and cerium (IV) sulfate as starting materials and aqueous ammonia solution as a precipitating agent. XRD data indicate that cerianite with face-centered cubic phase is formed in the reaction systems. The size of the coherent scattering regions is about 3 nm or less. FTIR spectroscopy data indicate the interaction of polymer molecules with the inorganic component. The shift of absorption bands related to N-H bonds for composites with Ce(III) and Ce(IV) compared to chitosan indicates the interaction of amino groups with CeO2 particles. The application of chitosan as a matrix for the synthesis of CeO2 nanoparticles showed that this approach is more economical and easier to produce nanomaterials for various applications.

Synthesis of chiral hollow polymer microspheres and their applications in the spectroscopic chiral discrimination of tryptophan isomers

Hollow polymer microspheres (HPMs) were synthesized, which were then hydrolyzed in aqueous ammonia to produce carboxyl (–COOH) groups on their surface. L-phenylalanine (L-Phe) was grafted to the hydrolyzed HPMs (H-HPMs) through amidation reactions, endowing the H-HPMs with chirality. The resultant chiral HPMs (C-HPMs) were used for the chiral discrimination of tryptophan (Trp) isomers. Due to the same rotatorydirection of L-Phe and L-Trp, the C-HPMs showed greatly higher selectivity toward L-Trp than its isomer. After being adsorbed by the C-HPMs, the absorbance of the residual L-Trp is significantly lower than that of the residual D-Trp, and thus spectroscopic chiral discrimination of the Trp isomers was successfully achieved. The Trp isomers were also discriminated by the chiral solid polymer microspheres (C-SPMs), while the difference in the absorbance of the residual L-Trp and D-Trp is remarkably smaller than that obtained by the C-HPMs. The outstanding discrimination capability of the C-HPMs might be ascribed to their high surface permeability resulted from their unique hollow structure.

Enhanced superconducting qubit performance through ammonium fluoride etch

Abstract The performance of superconducting qubits is often limited by dissipation and two-level systems (TLS) losses. The dominant sources of these losses are believed to originate from amorphous materials and defects at interfaces and surfaces, likely as a result of fabrication processes or ambient exposure. Here, we explore a novel wet chemical surface treatment at the Josephson junction-substrate and the substrate-air interfaces by replacing a buffered oxide etch (BOE) cleaning process with one that uses hydrofluoric acid followed by aqueous ammonium fluoride. We show that the ammonium fluoride etch process results in a statistically significant improvement in median T1 by ~22% (p=0.002), and a reduction in the number of strongly-coupled TLS in the tunable frequency range. Microwave resonator measurements on samples treated with the ammonium fluoride etch after niobium deposition and etching also show ~33% lower TLS-induced loss tangent compared to the BOE treated samples. As the chemical treatment primarily modifies the Josephson junction-substrate interface and substrate-air interface, we perform targeted chemical and structural characterizations to examine materials differences at these interfaces and identify multiple microscopic changes that could contribute to decreased TLS.

Preparation of TiO2 nanoparticles decorated porous carbon via a pseudo co-templating strategy and their application as substrates for high performance cathode of Li[sbnd]S batteries

Lithium‑sulfur batteries (LSBs) with a high theoretical specific capacity are considered as one of the most promising energy storage devices for next-generation. However, issues such as the insulation of pristine sulfur, the shuttle effect of polysulfides (LiPSs), and the volume change of sulfur cathode during cycling hinder their commercial applications. In this work, composite microspheres of TiO2@p-C consisting of porous carbon decorated with TiO2 nanoparticles, have been designed and synthesized aiming to confine and trap the polysulfides inside the porous substrates and to improve the kinetics of the electrochemical reaction therein. TiO2 particles with size of a few nanometers are prepared by a tetraethyl ammonium hydroxide assisted sol-gel method. Composite microspheres of TiO2@p-C are created through spray drying technique using chitosan as carbon source, and nanoparticles of SiO2 with TiO2 as co-template while selectively etching of the former. The confinement effects via the pores in the TiO2@p-C microspheres and the affinity between the polar TiO2 moieties and the polysulfide species work synergistically, alleviating the effusion of the polysulfides and promoting the conversion reaction of them as well. As a result, the shuttle effect of polysulfides can be inhibited obviously. Significantly, the S@TiO2@p-C-2 composite cathode exhibits excellent cyclic performance of 1263 mAh g−1 at 0.2C and maintains still a discharge capacity of 809 mAh g−1 after 100 charge and discharge cycles. At a current density of 2C, it still delivers an outstanding specific capacity of 772 mAh g−1. In the case of high sulfur load up to 90 wt%, the discharge capacity of S@TiO2@p-C-2 composite electrode could still maintain at 469.5 mAh g−1 after 100 cycles under current density of 0.2C.

Copper(II)-Catalyzed Amination of Aryl Chlorides in Aqueous Ammonia.

Anilines are ubiquitous in bio-active compounds and their synthesis can be achieved via metal-catalyzed cross-coupling reactions involving aryl halides. We describe an unusual, yet simple, CuII-catalyzed system for the amination of aryl chlorides in pure aqueous ammonia with 2.5 mol % catalyst loading under non-inert conditions. Different from previous systems, the reaction proceeds even without an additional organic solvent. Copper(II) sulfate in combination with 4,7-dimethoxy-1,10-phenanthroline enabled the amination of several aryl chlorides containing electron-neutral, -donating and -withdrawing groups to the corresponding anilines with good to excellent yields. The upscaling potential of the procedure has been shown by the synthesis at 50 mmol scale. The reaction proceeds as one of the rare cases of a CuII-assisted coupling, in contrast to the typical CuI-CuIII intermediates postulated for most Ullmann-type coupling reactions. The copper(II) center allows for a nucleophilic substitution pathway, enabled by the deprotonation of coordinated ammonia.

A Procedure for Solid-Phase Extractions Using Metal-Oxide-Coated Silica Column in Lipidomics.

Lipid enrichment is indispensable for enhancing the coverage of targeted molecules in mass spectrometry (MS)-based lipidomics studies. In this study, we developed a simple stepwise fractionation method using a titanium- and zirconium-dioxide-coated solid-phase extraction (SPE) silica column that separates neutral lipids, phospholipids, and other lipids, including fatty acids (FAs) and glycolipids. Chloroform was used to dissolve the lipids, and neutral lipids, including steryl esters, diacylglycerols, and triacylglycerols, were collected in the loading fraction. Second, methanol with formic acid (99:1, v/v) was used to retrieve FAs, ceramides, and glycolipids, including glycosylated ceramides and glycosylated diacylglycerols, by competing for affinity with the Lewis acid sites on the metal oxide surface. Finally, phospholipids strongly retained via chemoaffinity interactions were eluted using a solution containing 5% ammonia and high water content (45:50 v/v, 2-propanol:water), which canceled the electrostatic and chelating interactions with the SPE column. High average reproducibility of <10% and coverage of ∼100% compared to those of the non-SPE samples were demonstrated by untargeted lipidomics of human plasma and mouse brain, testis, and feces. The advantage of our procedure was showcased by characterizing minor lipid subclasses, including dihexosylceramides containing very long-chain polyunsaturated FA in the testis, monogalactosyl and digalactosyl monoacylglycerols in feces, and acetylated and glycolylated derivatives of gangliosides in the brain that were not detected using conventional solvent extraction methods. Likewise, the value of our method in biology is maximized during glycolipidome profiling in the absence of neutral lipids and phospholipids that cover more than 80% of the chromatographic peaks.

Surface-Modified Iron Oxide Nanoparticles with Natural Biopolymers for Magnetic Hyperthermia: Effect of Reducing Agents and Type of Biopolymers

Magnetic fluid hyperthermia, a minimally invasive localized therapy that uses heat generated by magnetic nanoparticles under an AC magnetic field, is a complementary approach for cancer treatment that is excellent due to its advantages of being noninvasive and addressing only the affected region. Still, its use as a stand-alone therapy is hindered by the simultaneous requirement of nanoparticle biocompatibility, good heating efficiency, and physiological safe dose. To overcome these limits, the biocompatible magnetic nanoparticles’ heating efficiency must be optimized. Iron oxide nanoparticles are accepted as the more biocompatible magnetic nanoparticles available. Therefore, in this work, superparamagnetic iron oxide nanoparticles were synthesized by a low-cost coprecipitation method and modified with starch and gum to increase their heating efficiency and compatibility with living tissues. Two different reducing agents, sodium hydroxide (NaOH) and ammonium hydroxide (NH4OH), were used to compare their influence. The X-ray diffraction results indicate the formation of a single magnetite/maghemite phase in all cases, with the particle size distribution depending on the coating and reducing agent. Citric acid functionalized water-based ferrofluids were also prepared to study the heating efficiency of the nanoparticles under a magnetic field with a 274 kHz frequency and a 14 kAm−1 amplitude. The samples prepared with NaOH display a higher specific loss power (SLP) compared to the ones prepared with NH4OH. The SLP value of 72 Wg−1 for the magnetic nanoparticles coated with a combination of starch and gum arabic, corresponding to an intrinsic loss power (ILP) of 2.60 nWg−1, indicates that they are potential materials for magnetic hyperthermia therapy.

Trypanosoma cruzi infection enhances olfactory response in Triatoma pallidipennis Stål (Hemiptera: Triatominae) to compounds potentially useful for insect control.

In Mexico, Triatoma pallidipennis is a major vector of Trypanosoma cruzi, the causative agent of Chagas disease. Current efforts are focused on developing attractants to control these vectors, using volatile substances derived from vertebrate hosts or compounds known to attract hematophagous insects. However, the efficacy of these compounds in attracting parasite-infected triatomines remains to be evaluated. In this study, we assessed the attractant activity of octenol (1-octen-3-ol), nonanal and a mixture of odorants consisting of ammonium hydroxide, lactic acid and hexanoic acid (in a ratio of 1:0.2:0.4 respectively), at concentrations of 1, 10 and 100 ng on the N3, N4 and N5 nymphal stages of T. pallidipennis, both infected and non-infected with T. cruzi. We also evaluated the synergistic effect of the most effective compounds and doses. All experiments were performed in a laboratory using a Y-type glass olfactometer. We found that both infected and non-infected N3 and N4 nymphs were attracted to low doses of octenol, nonanal and the odorant mixture. Particularly noteworthy was the synergistic effect observed between the odorant mixture and nonanal, which significantly increased attraction of T. cruzi-infected individuals. These findings contribute to the development of baited traps utilising these compounds for monitoring triatomines in epidemiological studies or for mass trapping to control these vectors.

Quantitation of Copper Tripeptide in Cosmetics via Fabric Phase Sorptive Extraction Combined with Zwitterionic Hydrophilic Interaction Liquid Chromatography and UV/Vis Detection

The increasing demand for effective cosmetics has driven the development of innovative analytical techniques to ensure product quality. This work presents the development and validation of a zwitterionic hydrophilic interaction liquid chromatography method, coupled with ultraviolet detection, for the quantification of copper tripeptide in cosmetics. A novel protocol for sample preparation was developed using fabric phase sorptive extraction to extract the targeted analyte from the complex cosmetic cream matrix, followed by chromatographic separation on a ZIC®-pHILIC analytical column. A thorough investigation of the chromatographic behavior of the copper tripeptide on the HILIC column was performed during method development. The mobile phase consisted of 133 mM ammonium formate (pH 9, adjusted with ammonium hydroxide) and acetonitrile at a 40:60 (v/v) ratio, with a flow rate of 0.2 mL/min. A design of experiments (DOE) approach allowed precise adjustments to various factors influencing the extraction process, leading to the optimization of the fabric phase sorptive extraction protocol for copper tripeptide analysis. The method demonstrated excellent linearity over a concentration range of 0.002 to 0.005% w/w for copper tripeptide, with a correlation coefficient exceeding 0.998. The limits of detection and quantitation were 5.3 × 10−4% w/w and 2.0 × 10−3% w/w, respectively. The selectivity of the method was verified through successful separation of copper tripeptide from other cream components within 10 min, establishing its suitability for high-throughput quality control of cosmetic formulations.

Extraction and Nutritional Value of Soybean Meal Protein Isolate

The increasing protein demand driven by global population growth necessitates the search for an alternative protein source. Soybean meal (SM), with approximately 47–49% proteins, is a viable option. Soymeal protein isolate (SMPI) is a cost-efficient protein source with a well-balanced amino acid profile, making it suitable for addressing this demand. This study attempts to address the lack of information regarding the extractability and nutritional potential of SMPI obtained utilizing a weak base and recoverable solvent. In this work, the structural and compositional studies of SMPI, as well as the aqueous extractability of ammonium hydroxide (NH4OH), are investigated. Furthermore, we examined the effects of heat treatment during oil extraction, where a comparison between protein isolates from soymeal and whole soybeans was carried out. The maximum extraction yield of 24.1% was achieved by increasing the concentration of NH4OH from 0.25–1%. In comparison to the soy protein isolate from whole soybeans (SPI), the compositional analysis report revealed that SMPI had higher levels of crude protein, moisture, and ash content, but lower levels of fat and carbohydrates. Furthermore, the examination of the amino acid composition confirms the existence of vital amino acids in SMPI. The amino acid score indicates that methionine, lysine, and threonine are the limiting amino acids. SMPI and SPI share structural and functional group similarities, as demonstrated by Fourier-transform infrared spectroscopy. Gel electrophoresis using sodium dodecyl sulfate–polyacrylamide shows that the protein molecular weight distributions of SPI and SMPI are similar. This in-depth evaluation emphasizes the advantages of SM by advocating its application in other sectors beyond conventional animal feed, such as nutritional supplements and bio-based products, and by improving the environmental sustainability and global food chains.

Nitrogen source regulates soil nitrification and nitrogen losses more than nitrification inhibitor and herbicide: A laboratory evaluation

AbstractIncreased environmental nitrogen (N) losses have prompted the use of enhanced efficiency nitrogen fertilizers, including nitrification inhibitors. However, the comparative effects of nitrification inhibitors with conventional nitrogen fertilizers and herbicides on soil nitrification and nitrogen losses remain poorly understood. This study evaluated the impact of a nitrification inhibitor (Instinct NXTGEN), two nitrogen sources (broadcast urea vs. injected aqueous ammonia), and a preemergence herbicide (Acuron) on (1) soil nitrification through a 25‐day soil incubation experiment and (2) NH3 volatilization, NO3‐N leaching, and N2O‐N emissions through a 31‐day soil column study in loamy sand soil. In both experiments, treatments included combinations of nitrification inhibitor versus no inhibitor, two nitrogen sources, and preemergence herbicide versus no herbicide. Results revealed that nitrogen source significantly influenced nitrification, with injected aqueous ammonia reducing nitrification by 33% compared to surface broadcast urea. Nitrification inhibitors and herbicide had no effect on soil nitrification. Injected aqueous ammonia reduced NH3 volatilization by 87% compared to surface broadcast urea, but the effect of the nitrification inhibitor on NH3 volatilization was inconsistent across both nitrogen sources. Injected aqueous ammonia led to 39% higher cumulative nitrogen (NO3‐N + NH4‐N) leaching than urea, while the nitrification inhibitor had an inconsistent effect on NO3‐N leaching across both nitrogen sources. No significant differences in N2O‐N emissions were observed among treatments, and the herbicide had no effect on any measured parameters. These findings suggest that nitrogen source plays a more critical role in regulating soil nitrogen losses than nitrification inhibitors or herbicides.