Amidopyrine Research Articles

BLOOD GLUCOSE AND CHOLESTEROL LEVELS OF BALI CATTLE THAT EXPERIENCED REPEATED BREEDING, IN THE VILLAGE OF SOBANGAN, MENGWI DISTRICT, BADUNG REGENCY, BALI

Previously, there have been many cases of suboptimal reproductive function in Bali cattle leading to repeated breeding. Blood biochemical profiles, such as blood glucose and cholesterol, can be parameters that describe body health, including the reproductive system. This study aims to determine the levels of blood glucose and cholesterol in Bali cattle that experience repeated mating. This study used an observational study with a completely randomized design (CRD) consisting of fertile and repeated mating Bali cattle groups. Sample determination was carried out based on criteria using a questionnaire, after which sampling was carried out, then an examination was carried out in the laboratory using the Glucose Oxidase-Phenol Amino Phenazone (GOD-PAP) and Cholesterol Oxidase-Peroxidase Aminoantypirin (CHOD-PAP) methods. The results showed that the average blood glucose levels in fertile and repeated mating Bali cattle were 85.55 mg/dL and 99.97 mg/dL, respectively. The average blood cholesterol levels of fertile and repeated mating Bali cattle were 131.84 mg/dL and 149.56 mg/dL, respectively. There was no significant difference between the fertile and repeated mating groups. It was concluded that the average blood glucose and cholesterol levels between fertile and repeated mating Bali cattle in this study did not affect the occurrence of repeated mating. Further research is needed on a larger scale.

Adsorption performance of activated bentonite, zeolite 13X and activated carbon for aminophenazone in transformer oil

Aminophenazone (AP) is one kind of harmful additives in transformer oil. It results in a significant drop of the insulation property of transformer oil and thus leads to substation accidents. Adsorption method has proved to be an efficient approach for AP removal. In this work, activated bentonite, zeolite 13X, and activated carbon were chosen as adsorbents for the separation of AP from the polluted transformer oil. At 25 ℃ and initial AP content is 400 ppmw, the AP uptake of activated bentonite (151.2 mg/g) is much higher than that of zeolite 13X (52.1 mg/g) and activated carbon (45.0 mg/g). The desorption activation energy (Ed) of AP on the three adsorbents follow in the order: activated bentonite (115.73 kJ/mol) > activated carbon (52.51 kJ/mol) > zeolite 13X (51.23 kJ/mol). Activated bentonite exhibits the highest AP uptake due to the existence of acidic sites that can strongly interact with alkaline AP and the pore sizes that facilitate AP molecules to more easily enter into the adsorbent channels. Activated bentonite is an industrially promising adsorbent for the removal of AP from the polluted transformer oil.

UJI EFEKTIFITAS SINBIOTIK KEFIR TEPUNG PISANG BATU TERHADAP KADAR GLUKOSA DARAH DAN KADAR PROFIL LIPID TIKUS MODEL SINDROM METABOLIK Effectiveness Test of banana (Musa balbisiana) Flour Kefir Synbiotic on Blood Glucose Level and Lipid Profile Level in Metabolic Syndrome Rats Model

Metabolic Syndrome is a metabolic disorder characterized by hyperglycemia, hypertriglyceridemia, and dyslipidemia which can be repaired through synbiotics. This study is to analyzed the effects of banana (Musa balbisiana) flour kefir synbiotic on blood glucose level and lipid profile level of Metabolic Syndrome rats. This is true experimental study with pre-posttest with control goup design of 24 male Sprague Dawley rats which were divided into 4 groups randomly. Negative control goup (K-) given standard food only, positive control group (K +) given standard food with High Fat Fructose Diet (HFFD), intervention group I (PI) and intervention group II (PII) were given standard food with HFFD and banana (Musa balbisiana) flour kefir synbiotic 1,8 ml/200 g weight/day (PI) and 3,6 ml/200 g weight/day (PII) for three weeks. Blood glucose level was analyzed using glucose oxidase-peroxidase amino antipyrine method, cholesterol level was analyzed using cholesterol oxidase-peroxidase amino antipyrine, and triglyceride level was analyze using glycerol 3 phosphate oxidase phenol amino phenazone method. Result of Analysis of Varian test shows significant differences in mean between group (p=0,000) followed by PostHoc Bonferroni test or Post Hoc Games-Howel test which showed a mean difference in the PI and PII groups. Banana (Musa balbisiana) flour kefir synbiotic can reduce blood glucose level and improve lipid profile level.

Effect of tomato and red guava juice on blood glucose level in overweight woman

Background: Based on the results of Riskesdas 2018 that the prevalence of diabetes mellitus in Indonesia showed an increase from 6.9% to 8.5%. Overweight is closely related to impaired blood glucose, insulin resistance, and decreased insulin secretion. Preventive efforts that have been made using non-pharmacological treatments, such as increasing the intake of fiber and lycopene from fruits.Objectives: This study aimed to determine the effect of tomato juice and guava juice on blood glucose levels in overweight women.Materials and Methods: The study design is a true experiment conducted in 11subjects in treatment group and 11 subjects in control group. The subject of this study were overweight adult women 45-55 years. Blood sampling was taken in the morning, then glucose levels were measured using the GOD-PAP method (Glucose Para Amino Phenazone). We gave 600 ml of tomato juice and red guava every day for 21 days. To find the effect of juice on fasting blood glucose levels controlled by nutrient intake and physical exercise using the Repeated Measure ANOVA test.Results: In the treatment group, there was a decrease of 3.24 mg/dl in blood glucose levels; while, in the control group, the decrease in blood glucose levels was only 0.26 mg/dl. However, we found no statistically significant differences in both groups.Conclusions: Consumption of tomato juice and red guava reduced fasting blood glucose.

PENURUNAN KADAR KOLESTEROL TOTAL DAN TRIGLISERIDA DENGAN TEKNIK BEKAM PADA PENDERITA HIPERKOLESTEROLEMIA

Background : Cupping is one type of natural treatment by suctioning the skin, cutting and removing blood from the skin surface. Cupping works for various diseases, can cure acute, chronic and degenerative diseases, such as high blood pressure and hypercholesterolemia. Cupping therapy has been carried out since thousands of years BC. Cupping consists of dry cupping and wet cupping. Method : This study used a pre-experimental design method for the One Group Pre test Post test. The examination method for Total cholesterol and Triglyceride levels was carried out using the CHOD - PAP (Cholesterol Oxydase Para Amino Phenazone) method and GPO - PAP (Glycerol-3-Phosphate Oxydase Para Amino Phenazone). Data were analyzed by T-Test. Result : Total cholesterol levels before wet cupping 231 mg / dl and after wet cupping therapy 220 mg / dl (p = 0.000) (r = 0.803) and Triglyceride levels before wet cupping 184 mg / dl and after wet cupping therapy 159 mg / dl (p = 0,000) (r = 0.901). There is a relationship between wet cupping therapy with a decrease in Total Cholesterol and Triglycerides in hypercholesterolemic patients in the working area of the Pinyuh River Inpatient Health Center. Conclusion : For the development of science it is recommended that further research be conducted on the effects of wet cupping therapy on clinical chemical parameters such as creatinine, gout, blood glucose levels, and other blood parameters.

Role of Ketotifen on metabolic profiles, inflammation and oxidative stress in diabetic rats.

We aim to explore effects of Ketotifen on metabolic profiles, inflammation and oxidative stress. Sprague Dawley (SD) male rats were randomly divided into normal control group (NC) and experimental groups, and experimental group rats were fed with high-sugar and fat diet for 6 weeks. Then, experimental group rats were divided into diabetes group (DM) and ketotifen treatment group (KT). KT group was given ketotifen via Intragastric for 8 weeks with the dosage of 0.09 mg/kg/d. Fasting plasma glucose (FPG) was measured using glucose oxidase-phenol amino phenazone method. Fasting insulin (FINS), total cholesterol (TC), triglyceride (TG), low-density lipoprotein (LDL), high-density lipoprotein (HDL), interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) were tested by enzyme-linked immunosorbent assay. Malondialdehyde (MDA) and superoxide dismutase (SOD) were quantified by spectrophotometer method. Before Ketotifen administration, compared with NC group, DM and KT groups showed significantly high levels of body weight, FPG, FINS, HOMA-IR, TC, TG, LDL, IL-6, TNF-α and MDA, and lower levels of HDL and SOD (All p <0.05). After 4 weeks of Ketotifen administration, levels of body weight, FPG, FINS, HOMA-IR, TC, TG, LDL, IL-6, TNF-α in KT group decreased significantly, and levels of HDL and SOD elevated significantly (All p <0.05). After 8 weeks of Ketotifen administration, levels of body weight, FPG, FINS, HOMA-IR, TC, TG, LDL, IL-6, TNF-α and MDA in KT group decreased more obviously, and levels of HDL and SOD elevated significantly further (All p <0.05). Ketotifen improved metabolic profiles, and ameliorated status of inflammation and oxidative stress.

Amidopyrine disposition in rat.

Amidopyrine disposition in rat.

NMR-Derived Models of Amidopyrine and Its Metabolites in Complexes with Rabbit Cytochrome P450 2B4 Reveal a Structural Mechanism of Sequential N-Dealkylation

To understand the molecular basis of sequential N-dealkylation by cytochrome P450 2B enzymes, we studied the binding of amidopyrine (AP) as well as the metabolites of this reaction, desmethylamidopyrine (DMAP) and aminoantipyrine (AAP), using the X-ray crystal structure of rabbit P450 2B4 and two nuclear magnetic resonance (NMR) techniques: saturation transfer difference (STD) spectroscopy and longitudinal (T(1)) relaxation NMR. Results of STD NMR of AP and its metabolites bound to P450 2B4 were similar, suggesting that they occupy similar niches within the enzyme's active site. The model-dependent relaxation rates (R(M)) determined from T(1) relaxation NMR of AP and DMAP suggest that the N-linked methyl is closest to the heme. To determine the orientation(s) of AP and its metabolites within the P450 2B4 active site, we used distances calculated from the relaxation rates to constrain the metabolites to the X-ray crystal structure of P450 2B4. Simulated annealing of the complex revealed that the metabolites do indeed occupy similar hydrophobic pockets within the active site, while the N-linked methyls are free to rotate between two binding modes. From these bound structures, a model of N-demethylation in which the N-linked methyl functional groups rotate between catalytic and noncatalytic positions was developed. This study is the first to provide a structural model of a drug and its metabolites complexed to a cytochrome P450 based on NMR and to provide a structural mechanism for how a drug can undergo sequential oxidations without unbinding. The rotation of the amide functional group might represent a common structural mechanism for N-dealkylation reactions for other drugs such as the local anesthetic lidocaine.

Protective action of antioxidants and microsomal mono-oxygenase inducers in ischemic and reoxygenation liver damage

The writers showed previously that endogenous products of lipid peroxidation (LPO) accumulate in the liver in acute ischemia, and this correlates with depression of microsomal mono-oxygenase activity; it has been postulated that LPO processes participate in ischemic damage to NADPH-dependent oxygenases of the endoplasmic reticulum (ER) [5, 6]. It has also been shown that antioxidants inhibit accumulation of LPO products in ischemia and reoxygenation of different organs [i, 3, 9, i0] and that inducers of microsomal mono-oxygenases are responsible for preservation of a higher concentration of cytochrome P-450 and of the rate of N-demethylation of amidopyrine (AP) in ischemia of the liver [12].

Synthesis of some 2‐(N‐protected or free aminoacyl or N‐tosyltripeptide)‐aminophenazines, aminonaphthophenazines and 4‐(N‐protected or free aminoacyl or N‐tosyltripeptide)aminophenazone derivatives

AbstractThe syntheses of different 2‐(N‐tosyl‐ or N‐phthalyl‐ or free aminoacyl or N‐tosyltripeptide)‐aminophenazines, and the corresponding derivatives of 2‐aminonaphthophenazines and some derivatives of 4‐aminophenazones (IV‐XXXIII) are described. Compounds XI‐XIII, XX‐XXIII, XXX‐XXXII, XXXIV‐XXXIX and XL‐XLV were found to be active against a number of microorganisms.

The oxidation of aminophenazone and phenazone by ferricyanide

The oxidation of aminophenazone and phenazone by ferricyanide

New method for preparing amidopyrine tablets

New method for preparing amidopyrine tablets

Necrosis of the Rectum Complicating Agranulocytosis due to Amidopyrine

Necrosis of the Rectum Complicating Agranulocytosis due to Amidopyrine

Amidopyrine and agranulocytosis.

Amidopyrine and agranulocytosis.

Amidopyrine as an antispasmodic.

Amidopyrine as an antispasmodic.

Agranulocytosis caused by amidopyrine; an avoidable cause of death.

Agranulocytosis caused by amidopyrine; an avoidable cause of death.

Agranulocytosis and sensitization to pyramidon: Disselmeyer, H., and Zorn, O.: München. med. Wchnschr. 58: 247, 1937

Agranulocytosis and sensitization to pyramidon: Disselmeyer, H., and Zorn, O.: München. med. Wchnschr. 58: 247, 1937

Agranulocytosis and Amidopyrine

Agranulocytosis and Amidopyrine

Allergy to Amidopyrine: Blood Studies Following Anaphylactic-like Shock in a Patient

Allergy to Amidopyrine: Blood Studies Following Anaphylactic-like Shock in a Patient

Agranulocytosis Fatalities from Amidopyrine and Other Drugs

Agranulocytosis Fatalities from Amidopyrine and Other Drugs