Thirty seven diploid and 7 haploid rice callus cultures were induced from the World Rice Collection maintained in Hungary. Cell suspension cultures were started from these calli in LS-2,5 liquid medium and subsequently transferred into amino acid (AA) medium. After 1 year of culturing 20 out of the 44 genotypes were found suitable for protoplast isolation. So far protoplast derived calli of genotypes have been obtained by culturing in RY-2 protoplast medium, and protoplast derived green plants of 3 genotypes have been regenerated through a two-step regeneration procedure. The protoplast derived plants are grown in pots under greenhouse conditions. Experiments are being carried out with the other genotypes developing the plant-protoplast-plant system into a general method which is not dependent on the genotype.