Quercus Research Articles

Cytisus Legume Association Interests on Oak Growth and Cork Quality

Background: The cork oak is the major forest element of northern Tunisia, especially in Kroumirie. It is a species of greatecological, economic and social value, with a natural distribution limited to the western Mediterranean basin. Following serious biotic and abiotic problems, oak forests have been greatly affected and their natural regeneration has become very difficult, even almost absent these last years marked by last climatic changes. The association of cork oak with autochthonous plant species such as leguminous is an original research to resolve this problem. As part of this reflection, the association of the cork oak with Cytisusvillosus could positively influence its growth and productivity by providing the nutrients necessary for its survival in the face of climatic constraints. Methods: The study was conducted in two sites in the Kroumirie (Ain Draham and El Feija forests). In each site two populations of the same bioclimatic stage are chosen: a population of only cork oak and a cork oak population associated with Cytisusvillosus. Several parameters were measured on trees at each population: morphological (height, circumference, leaf area index LMA) ecophysiological (photosynthesis, stomatal conductance, transpiration) and cork quality (porosity, humidity, quality) parameters. Result: The oaks with legumes in both Ain Drahem and Feija sites showed highly significant differences compared to the other two populations without association; for morphological and ecophysiological parameters. In the same way, statistical analysis has shown that the cork oak trees in Feija population with Cytisus have better cork quality than other populations. We can conclude that the cork oak behaves better and have higher growth and better cork quality in the presence of legumimous species like Cytisusvillosus. Such results could encourage the reintroduction of this legume especially in degraded areas of cork oaks for their maintenance and preservation especially in the current climate conditions.

Interspecific niche overlap and climatic associations of native Quercus species in the Zagros forests of Iran

In the arid regions surrounding southwestern Asia, oak trees play an important role in the environment by conserving water and preserving soil and supporting wildlife. There are concerns for the health and continued productivity of these forests, and there is a widespread oak decline. In this study, ecological niche modeling (ENM) and multivariate principal component analysis (PCA) were used to determine the environmental variations among Quercus brantii, Q. infectoria, and Q. libani in the Zagros forests of western Iran. Based on the area under the curve (AUC > 0.9) and the true skill statistic (TSS > 0.8), three algorithms, including random forest (RF), maximum entropy (MaxEnt), and maximum picking neural net (MAXnet), were used in an ensemble approach to explore the most important environmental variables within the projected suitable habitat of each species. Environmental variables related to climate, including annual precipitation (bio12) and annual mean temperature (bio1), were among the most important drivers of habitat suitability for oak species. The results illustrated that suitable areas for Q. infectoria and Q. libani often occur in the northern parts of the Zagros, which are humid and cooler than the southern areas. The results suggested that Q. brantii has a higher tolerance for harsh conditions in Zagros and therefore inhabits almost all areas of Zagros, including the southern parts. The climatic overlap between pairs of species was found to be significant based on equivalency and similarity tests, suggesting that the environmental conditions of the two species compared are more similar than would be expected by chance. The gap analysis for the three oak species revealed that only a few protected areas currently exist within their suitable habitats, with most of the highly suitable habitats located on non-protected lands, particularly for Q. libani and Q. infectoria. In addition, deforestation is known to sometimes occur within the boundaries of protected areas. Improving the level of protection by upgrading the current protected areas to national parks can help with conservation.

Multivariate analysis and GIS approaches for modeling and mapping soil quality and land suitability in arid zones

Assessing soil quality marks the initial step in precision farming and agricultural management. Developing countries like Egypt face numerous hurdles in ensuring food security due to increasing populations and limited agricultural resources. A geographic information system (GIS) and multivariate analysis were utilized in the current work to evaluate and map a soil quality index (SQI). Moreover, the land suitability of the land for two plantations of the tree's oak (Quercus robur), and pine (Pinus silvestris), respectively was assessed using a parametric approach. A total of 82 soil profiles were selected to fulfill the objectives of the study. Based on the samples' PC scores, and agglomerative hierarchical clustering (AHC, the data was divided into two clusters: Cluster I and Cluster II, which collectively account for approximately 57% and 43% of the total data, respectively.. . The findings indicated that land suitability for planting Q. robur planted identified 2.14% of the research area as highly suitable (S1), 37.98% as moderately suitable (S2), and 59.89% as not suitable (N). Furthermore, the assessment of suitability for P. silvestris indicated that 50.88% of the investigated area was classified into: S1, 48.73% as S2, and 0.39% as N, which means it is not suitable for conservation activities. The research identified that soil depth beside excessive salinity and calcium carbonate as the primary soil constraints in the area in both clusters. The average soil depth, ECd and CaCO3 were 113.62 ± 12.41, 17.27 ± 10.23, 16.83 ± 6.57 in Cluster 1 and 45.43 ± 15.21, 22.42 ± 12.43, 21.55 ± 5.63 in Cluster II. The study demonstrates that integrating multivariate analysis with GIS enables a precise and streamlined assessment of the Soil Quality Index (SQI). Soil suitability modelling underscores the importance of implementing efficient management practices to attain agricultural sustainability in arid regions, particularly amidst intensive land utilization pressures

Divergence and reticulation in the Mexican white oaks: ecological and phylogenomic evidence on species limits and phylogenetic networks in the Quercus laeta complex (Fagaceae).

Introgressive hybridization poses a challenge to taxonomic and phylogenetic understanding of taxa, particularly when there are high numbers of co-occurring, intercrossable species. The genus Quercus exemplifies this situation. Oaks are highly diverse in sympatry and cross freely, creating syngameons of interfertile species. Although a well-resolved, dated phylogeny is available for the American oak clade, evolutionary relationships within many of the more recently derived clades remain to be defined, particularly for the young and exceptionally diverse Mexican white oak clade. Here, we adopted an approach bridging micro- and macroevolutionary scales to resolve evolutionary relationships in a rapidly diversifying clade endemic to Mexico. Ecological data and sequences of 155 low-copy nuclear genes were used to identify distinct lineages within the Quercus laeta complex. Concatenated and coalescent approaches were used to assess the phylogenetic placement of these lineages relative to the Mexican white oak clade. Phylogenetic network methods were applied to evaluate the timing and genomic significance of recent or historical introgression among lineages. The Q. laeta complex comprises six well-supported lineages, each restricted geographically and with mostly divergent climatic niches. Species trees corroborated that the different lineages are more closely related to other species of Mexican white oaks than to each other, suggesting that this complex is polyphyletic. Phylogenetic networks estimated events of ancient introgression that involved the ancestors of three present-day Q. laeta lineages. The Q. laeta complex is a morphologically and ecologically related group of species rather than a clade. Currently, oak phylogenetics is at a turning point, at which it is necessary to integrate phylogenetics and ecology in broad regional samples to figure out species boundaries. Our study illuminates one of the more complicated of the Mexican white oak groups and lays groundwork for further taxonomic study.

Comparative Spatial Modeling of Bretziella fagacearum Distribution in Northern Wisconsin, United States.

Bretziella fagacearum, the ascomycete fungus oak wilt, is considered a virulent threat to North American oaks, but the influence of the physical environment on this pathosystem remains unclear, particularly at the forest scale. This study explored the influence of terrain and soil factors on B. fagacearum infections, applying discrete and continuous spatial models to investigate the question, besides proximity to other infections, which environmental factors influenced B. fagacearum incidence? Locations of infections were recorded from 586 confirmed B. fagacearum sites, identified from 2004 through 2021 in a 76 km2 area of deep, sandy glacial outwash in Chequamegon-Nicolet National Forest, northern Wisconsin. Public datasets derived from remote sensing were incorporated as covariates, describing terrain elevation (USGS 10-m DEM), soil physical and chemical properties (POLARIS), and forest composition (WiscLand2). Spatial models included generalized additive models (GAMs) and Neyman-Scott cluster process models. The results indicated that spatial dependence and the distribution of oak forests were the most important drivers of B. fagacearum distribution in this area, with more minor influence from elevation, hill shade, and drainage patterns. Comparison between modeling approaches indicated that-at this scale and in this area-the most accurate models were those that included host distribution, spatial dependence, and quantitative terrain and soil descriptions. However, a close approximation could be attained using nonlinear models (GAMs) that incorporated only host distribution and spatial dependence.

Firebrands Generated During WUI Fires: A Novel Framework for 3D Morphology Characterization

The goal of the present work is to establish a framework for firebrand morphology characterization. Central to this framework is the development of a simple firebrand shape classification model using multi-dimensional particle shape descriptors. This classification model is built upon a series of synthetically generated 3D particles whose shapes and sizes are chosen to be representative of actual firebrands typically encountered during vegetative and structural fuel burns. Principal Component Analysis (PCA) is applied to the synthetic dataset and used to structure the classification model. The model is then verified using 3D digital representations of real-world particles (firebrands collected during tree burns and unburned bark pieces from oak trees). The classification model, which will allow meaningful comparisons of firebrand morphological features by shape class, is expected to be gradually refined as more datasets are made available throughout the Wildland–Urban Interface (WUI) fire research community.

First report of Diplodia quercivora causing branch cankers on declining chestnut oak (Quercus montana) in Virginia.

Since the beginning of the twentieth century oak decline has been documented in central and eastern hardwood forests of the United States as a stress-mediated disease (Oak et al. 2016). Opportunistic canker pathogens, including Diplodia corticola, D. quercivora, D. sapinea, and Botryosphaeria dothidea have been associated with crown dieback of declining oak trees in several mid-Atlantic states (Ferreira et al. 2021). On 02 August 2022, a survey was conducted at two natural hardwood sites in Fredrick and Shenandoah Counties, Virginia that exhibited symptoms of decline (Fig. 1A). At both sites, mature Quercus montana trees were observed with bole and branch cankers, bleeding and sooty lesions, and discolored sapwood. Pycnidia were present on the margin of seven branch cankers from three trees that were felled, with hyaline, elliptical to oblong conidia 19.0 - 26.8 × 8.5 - 11.2 µm (n = 40) in size (Fig. 1C and D). Six cultures were derived from single spores that were placed on PDA medium and incubated for 10 days in the dark at 22 ± 2°C. Additionally, a 4-mm piece of necrotic tissue was selected from the margin of each of the seven cankers, disinfected with 2.5% NaOCl, again with 70% ethanol, and air-dried before being placed on half-strength acidified PDA medium (pH 4.8) and incubated in the dark at 22 ± 2°C. After 5 days, seven colonies from each canker assayed were transferred to full-strength PDA plates and incubated for 10 days in the dark at 22 ± 2°C. Colonies derived from spores and the necrotic wood were morphologically identical, with white, aerial, floccose mycelium that turned dark gray to olivaceous after five days (Fig. 1B). DNA was individually extracted from four, 10-day-old cultures (two from spores and two from wood). Mycelia was harvested with a sterile pin and extracted using a Qiagen DNeasy Plant Pro Kit (Germantown, MD) according to the manufacturer's instructions. A segment of the internal transcribed spacer (ITS), large subunit rRNA (LSU), and translation elongation factor 1-α (tef1) loci were amplified using ITS4/ITS5 (White et al. 1990), LR5/LROR (Vilgalys and Hester 1990), and EF1-728F/EF1-986R (Carbone and Kohn 1999) primer sets, respectively. The PCR amplicons were purified with ExoSap-IT (Affymetrix, Santa Clara, CA) and sequenced at Eurofins (Louisville, KY). The nucleotide sequences were analyzed using Geneious 11.1.5 software (Biomatters, Auckland, NZ). The resulting ITS sequences from the four isolates were identical. A 544-bp, 1131-bp, and 273-bp segment of the ITS, LSU, and tef1 loci from isolate GS22-DSB-17 was deposited into the GenBank database (accessions OQ597712, OQ597714, and OR754429, respectively). A Genbank BLAST analysis revealed that the ITS and tef1 fragments shared 510/516 (99%) and 271/273 (99%) nucleotides with the D. quercivora ex-type BL8 (JX894205/JX894229). Koch's postulates were fulfilled by inoculating five healthy, containerized Q. montana trees (average stem caliper 6.5 cm) with D. qercivora isolate GS22-DSB-17, while five plants were used as controls. After disinfecting the bark with 70% ethanol, a 0.5 mm section of the bark was removed 13 cm above the soil line with a sterile scalpel, and a 0.5 mm agar plug taken from the edge of a 10-day-old PDA culture was placed in the wound with the mycelium facing the cambial tissue, sealed with Parafilm, and maintained at 22 ± 6°C. The same procedure was performed on the control plants using sterile PDA plugs. After six weeks the bark was carefully removed, and all five stems treated with D. quercivora had necrotic lesions with a mean canker linear growth ([length+width]/2) of 15.6 mm from the edge of the wound, which was significantly larger (P = 0.001) than the controls (2.3 mm; Fig. 1E-M). Necrotic stem tissue was sampled as previously described, and the isolate recovered was confirmed as D. quercivora based on morphology and 100% ITS sequence homology to isolate GS22-DSB-17. D. quercivora was not recovered from the control plants. In the United States, D. quercivora has been isolated from declining white oak trees in Maryland, Massachusetts, West Virginia, and Florida (Dreaden et al. 2014; Ferreira et al. 2021; Haines et al. 2019). More surveys are needed to understand the host range and distribution of D. quercivora in the United States, as well as the environmental and site factors that impact oak health and predispose trees to infection from opportunistic cankering pathogens.

Alginate-encapsulated biochar as an effective soil ameliorant for reducing Pb phytoavailability to lettuce (Lactuca sativa L.).

The alginate-biochar formulation for metal removal from aquatic environments has been widely tried but its use for lowering phytoavailability of metals in the soil-crop continuum is limited. Biochar has been increasingly used as a soil amendment due to its potential for soil carbon sequestration and sorption capacity. Handling of powdery biochar as a soil top-dressing material is, however, cumbersome and vulnerable to loss by water and wind. In this experiment, biochar powder, which was pyrolyzed from oak trees, was encapsulated into beads with alginate, which is a naturally occurring polysaccharide found in brown algae. Both batch and pot experiments were conducted to examine the effects of the alginate-encapsulated biochar beads (BB), as compared to its original biochar powdery form (BP), on the Pb adsorption capacity and phytoavailability of soil Pb to lettuce (Lactuca sativa L.). The BB treatment improved reactivity about six times due to a higher surface area (287 m2g-1) and five times due to a higher cation exchange capacity (50 cmolc kg-1) as compared to the BP treatment. The maximum sorption capacity of Pb was increased to 152 from 81mgg-1 because of surface chemosorption. Adsorption of Pb onto BB followed multiple first-order kinetics and comprised fast and slow steps. More than 60% of the Pb was adsorbed in the fast step, i.e., within 3h. Also, the BB treatment, up to the 5% level (w/w), increased soil pH from 5.4 to 6.5 and lowered the phytoavailable fraction of Pb in soil from 5.7 to 0.3mgkg-1. The Pb concentrations in lettuce cultivated at 5% for the BP and BB treatments were similar but 63 and 66% lower, respectively, than those of the control soil. The results showed that the encapsulation of biochar with alginate enhanced adsorption by the biochar.

Impacts of elevated CO2 levels and temperature on photosynthesis and stomatal closure along an altitudinal gradient are counteracted by the rising atmospheric vapor pressure deficit

The efficiency of water use in plants, a critical ecophysiological parameter closely related to water and carbon cycles, is essential for understanding the interactions between plants and their environment. This study investigates the effects of ongoing climate change and increasing atmospheric CO2 concentration on intrinsic (stomata-based; iWUE) and evaporative (transpiration-based; eWUE) water use efficiency in oak trees along a naturally small altitudinal gradient (130–630 m a.s.l.) of Vihorlat Mountains (eastern Slovakia, Central Europe). To assess changes in iWUE and eWUE values over the past 60 years (1961–2020), stable carbon isotope ratios in latewood cellulose (δ13Ccell) of annually resolved tree rings were analyzed. Such an approach was sensitive enough to distinguish tree responses to growth environments at different altitudes. Our findings revealed a rising trend in iWUE, particularly in oak trees at low and middle altitudes. However, this increase was negligible at high altitudes. Warmer and drier conditions at lower altitudes likely led to significant stomatal closure and enhanced efficiency in photosynthetic CO2 uptake due to rising CO2 concentration. Conversely, the increasing intracellular-to-ambient CO2 ratio (Ci/Ca) at higher altitudes indicated lower efficiency in photosynthetic CO2 uptake. In contrast to iWUE, eWUE showed no increasing trends over the last 60 years. This suggests that the positive impacts of elevated CO2 concentrations and temperature on photosynthesis and stomatal closure are counteracted by the rising atmospheric vapor pressure deficit (VPD). These differences underscore the importance of the correct interpretation of stomata-based and transpiration-based WUEs and highlight the necessity of atmospheric VPD correction when applying tree-ring δ13C-derived WUE at ecosystem and global levels.

Reliable and specific detection and identification of Brenneria goodwinii, the causal agent of oak and oriental beech decline

Chestnut-leaved oak (Quercus castaneifolia) and oriental beech (Fagus orientalis) are among the major tree species in the Hyrcanian forests. Brenneria goodwinii was identified as the causal agent of necrotic lesions and stem bleeding on affected oak trees in different countries. Oak and oriental beech trees with bleeding symptoms were observed in a few forest sites in northern Iran. The objectives of the present study were to identify and characterize the causal agents of bark canker in oak and oriental beech trees and develop a primer set for specific detection, using polymerase chain reaction (PCR), of Brenneria goodwinii strains. A total of 31 and 20 samples from oak and oriental beech trees, respectively, with stem bleeding and bark canker symptoms were collected from Golestan and Mazandaran forests in northern Iran in 2020–2021. Bacterial strains displaying a green metallic sheen on EMB-agar medium were isolated from symptomatic oak (105 strains) and oriental beech samples (32 strains), while 31 and 20 strains were also isolated from healthy oak and oriental beech, respectively. Pathogenicity tests indicated that 51 and 25 strains isolated from oak and oriental beech, respectively were able to induce a necrotic area on oak acorns 15 days following inoculation. Moreover, four and two representative strains inoculated on oak and oriental beech twigs, respectively induced necrosis on all inoculated green twigs 1 month after inoculation. The sequences of the 16S rRNA and gyrB genes of representative strains isolated from and proved pathogenic on oak and oriental beech trees were 100% and over 99% similar to B. goodwinii LMG 26270T, respectively, which revealed the strains belong to B. goodwinii species. The primer pair BgF3/R2, which was designed to target the hrpN gene, was proven to be specific in the detection of B. goodwinii strains. The primer pair amplified a 618-bp DNA fragment from strains of B. goodwinii only and not from strains belonging to Rahnella, Gibbsiella, Lonsdalea, and the other Brenneria species among several other pathogenic bacteria tested. No fragment was amplified from DNA extracted from healthy trees or seedlings in PCR using this primer pair.

<div>First record of saproxylic beetle Corticeus (= Hypophloeus) unicolor Piller & Mitterpacher, 1783 in Montenegro (Coleoptera: Tenebrionidae) with comments on old-growth forests conservation in the Country</div>

The occurrence of Darkling Beetle Corticeus (=Hypophloeus) unicolor Piller & Mitterpacher, 1783 (Coleoptera: Tenebrionidae), a saproxylic predator beetles, is reported for Montenegro. The species develops in freshly the dead wood of birch, beech, and oak trees. It is probably a predator of the larvae of the beetle Hylecoetus and other wood borers. C. unicolor was found in the Biogradska Gora National Park, in the north-western part of the Bjelasica mountain range in the Dinaric Alps (Montenegro), in October 2022 in an old-growth beech forest at about 1,000 m elevation.

Do Mature Quercus aliena Primarily Use Deep Soil Water?

Seasonal drought events induced by climate change have exacerbated the water deficit in trees. This is particularly pronounced in mature trees with considerable heights and extensive branches, where the long water transport distance leads to a reduction in hydraulic transportation efficiency. Therefore, investigating the water uptake patterns of mature trees is essential to enhance their growth and resilience to climate change. However, previous studies have predominantly focused on trees aged 10–60 years, with limited research on the water uptake patterns of trees over 60 years old. In this study, we employed hydrogen and oxygen stable isotopes coupled with the MixSIAR model to determine the water uptake patterns of mature Quercus aliena var. acuteserrata (40–60 years, 60–90 years, 120–150 years, >150 years) during the growing seasons of 2021 and 2022 in Baotianman Nature Reserve, Henan Province, China. Additionally, we utilized a random forest model to quantify the relative contributions of vegetation (fine root biomass) and soil properties (soil moisture, bulk density, total porosity, field capacity, and soil texture) to the water uptake patterns of Q. aliena. Our findings demonstrate that most mature Q. aliena predominantly extracted deep soil water (60–100 cm) in the early growing season, but shifted towards utilizing shallow soil water (0–40 cm) in the late growing season. The water uptake pattern of mature Q. aliena was primarily regulated by soil moisture. These results reveal that mature Q. aliena trees exhibit a flexible water use strategy, enabling them to cope with seasonal drought by altering the soil depth from which they extract water. In the future, in the process of the cultivation and conservation of mature oak trees, surface soil irrigation can be increased to prevent tree mortality resulting from water deficit during drought conditions.

Abiotic factors impact on oak forest decline in Lorestan Province, Western Iran

The Zagros oak forests in Iran are facing a concerning decline due to prolonged and severe drought conditions over several decades, compounded by the simultaneous impact of temperature on oak populations. This study in oak woodlands of central Zagros forests in Lorestan province analyzed abiotic factors such as climate properties, topographic features, land use, and soil properties from 1958 to 2022. We found that higher elevation areas with steeper slopes and diverse topography show significant potential for enhancing oak tree resilience in the face of climate change. Additionally, traditional land use practices like livestock keeping and dryland farming contribute to a widespread decline in oak populations. Preserving forest biodiversity and ensuring ecological sustainability requires immediate attention. Implementing effective land-use management strategies, such as protecting and regulating human-forest interaction, and considering meteorological factors to address this issue is crucial. Collaborative efforts from stakeholders, policymakers, and local communities are essential to oppose destructive suburban sprawl and other developments. Sustainable forestry practices should be implemented to improve the living standards of local communities that rely on forests and traditional livestock keeping, offer forestry-related jobs, and ensure social security. Such efforts are necessary to promote conservation awareness and sustainable practices, safeguarding this unique and vital ecosystem for future generations.

Complete Chloroplast Genomes of Four Oaks from the Section Cyclobalanopsis Improve the Phylogenetic Analysis and Understanding of Evolutionary Processes in the Genus Quercus.

Quercus is a valuable genus ecologically, economically, and culturally. They are keystone species in many ecosystems. Species delimitation and phylogenetic studies of this genus are difficult owing to frequent hybridization. With an increasing number of genetic resources, we will gain a deeper understanding of this genus. In the present study, we collected four Quercus section Cyclobalanopsis species (Q. poilanei, Q. helferiana, Q. camusiae, and Q. semiserrata) distributed in Southeast Asia and sequenced their complete genomes. Following analysis, we compared the results with those of other species in the genus Quercus. These four chloroplast genomes ranged from 160,784 bp (Q. poilanei) to 161,632 bp (Q. camusiae) in length, with an overall guanine and cytosine (GC) content of 36.9%. Their chloroplast genomic organization and order, as well as their GC content, were similar to those of other Quercus species. We identified seven regions with relatively high variability (rps16, ndhk, accD, ycf1, psbZ-trnG-GCC, rbcL-accD, and rpl32-trnL-UAG) which could potentially serve as plastid markers for further taxonomic and phylogenetic studies within Quercus. Our phylogenetic tree supported the idea that the genus Quercus forms two well-differentiated lineages (corresponding to the subgenera Quercus and Cerris). Of the three sections in the subgenus Cerris, the section Ilex was split into two clusters, each nested in the other two sections. Moreover, Q. camusiae and Q. semiserrata detected in this study diverged first in the section Cyclobalanopsis and mixed with Q. engleriana in the section Ilex. In particular, 11 protein coding genes (atpF, ndhA, ndhD, ndhF, ndhK, petB, petD, rbcL, rpl22, ycf1, and ycf3) were subjected to positive selection pressure. Overall, this study enriches the chloroplast genome resources of Quercus, which will facilitate further analyses of phylogenetic relationships in this ecologically important tree genus.

Endogenous rhythmic growth and ectomycorrhizal fungi modulate priming of antiherbivore defences in subsequently formed new leaves of oak trees

Abstract Priming of plant defences provides increased plant protection against herbivores and reduces the allocation costs of defence. Defence priming in woody plants remains obscure, in particular, due to plant development traits such as endogenous rhythmic growth. By using bioassays with oak microcuttings (Quercus robur) and combining transcriptomic and metabolomic analyses, we investigated how leaf herbivory by Lymantria dispar and root inoculation with the ectomycorrhizal fungus Piloderma croceum prime oak defences. We further investigated how defence priming is modulated by the rhythmic growth of the oaks. A first herbivory challenge in oak leaves primed newly grown leaves for enhanced induction of jasmonic acid (JA)‐related direct defences, or enhanced emission of volatiles, depending on the specific growth stage at which the plants were first challenged. Root inoculation with Piloderma abolished the enhanced induction of JA‐related defences and volatile emission. Synthesis: Our results indicate that a first herbivore attack primes direct and indirect defences of newly formed oak leaves and that the specific display of defence priming is regulated by rhythmic growth, and modulated by the interaction Piloderma. Our results show that the priming memory in oaks can be transmitted to the next growth cycle, to the leaves of the new shoot unit.

Decomposing drivers in avian insectivory: Large‐scale effects of climate, habitat and bird diversity

AbstractAimClimate is a major driver of large‐scale variability in biodiversity, as a likely result of more intense biotic interactions under warmer conditions. This idea fuelled decades of research on plant‐herbivore interactions, but much less is known about higher‐level trophic interactions. We addressed this research gap by characterizing both bird diversity and avian predation along a climatic gradient at the European scale.LocationEurope.TaxonInsectivorous birds and pedunculate oaks.MethodsWe deployed plasticine caterpillars in 138 oak trees in 47 sites along a 19° latitudinal gradient in Europe to quantify bird insectivory through predation attempts. In addition, we used passive acoustic monitoring to (i) characterize the acoustic diversity of surrounding soundscapes; (ii) approximate bird abundance and activity through passive acoustic recordings; and (iii) infer both taxonomic and functional diversity of insectivorous birds from recordings.ResultsThe functional diversity of insectivorous birds increased with warmer climates. Bird predation increased with forest cover and bird acoustic activity but decreased with mean annual temperature and functional richness of insectivorous birds. Contrary to our predictions, climatic clines in bird predation attempts were not directly mediated by changes in insectivorous bird diversity or acoustic activity, but climate and habitat still had independent effects on predation attempts.Main ConclusionsOur study supports the hypothesis of an increase in the diversity of insectivorous birds towards warmer climates but refutes the idea that an increase in diversity would lead to more predation and advocates for better accounting for activity and abundance of insectivorous birds when studying the large‐scale variation in insect‐tree interactions.

The variation of growth in diameter and its relationship with the height of pedunculated oak (Quercus Robur L.) trees in the provenance cultures

In provenance cultures, studies were conducted on diameter growth dynamics in pedunculated oak (Quercus robur L.) trees and the height-diameter relationship was evaluated based on three data approximation models. The studies highlighted a signifi cant variation of the trunk diameter in trees of different origins and a similar growth reaction of oaks from four provenances (Hârjauca, Plaiul Fagului, Zloti, and Baimaclia) in relation to the environmental conditions of the common stand. In all the years of observation, the trees from Edineț had a rapid growth in diameter, thus demonstrating their ability to adapt through phenotypic plasticity. An uneven distribution of tree diameters was observed by provenance, indicating that dominant trees have an advantage in competition for light, slowing the growth of those with smaller diameters and causing a positive skew towards oaks with larger diameters. With the advancing age of the oaks, a tendency to decrease the values of the heritability coefficients for the trunk diameter was observed. At the age of 5 years, studies show a strong genetic control over the growth in diameter, a fact highlighted by the heritability coefficient value of 0,53 for this character. As the trees age, non-genetic factors such as environmental conditions become increasingly important in determining tree diameter growth. The comparative analysis of three statistical fitting models for data on pedunculate oak trees from different provenances, showed that the logarithmic model is the most appropriate to explain the relationship between their height and diameter.

Changes in leaf lifespan, nitrogen resorption, and mean residence time of leaf nitrogen along a soil fertility gradient in an evergreen oak tree.

The ability of plants to retain nitrogen (N) for a long period of time is critical to their N use efficiency, growth, and fitness, particularly in infertile environments. The mean residence time of leaf N (MRTL) and its two determinants, leaf lifespan and N resorption efficiency (rN, the fraction of the total leaf N pool that is resorbed during leaf senescence), have been hypothesized to increase plastically with decreasing soil N fertility but this remains to be fully tested. To avoid confusion by random changes in these characteristics in a relatively narrow N fertility range, MRTL, leaf lifespan, and N resorption efficiency were measured in Quercus glauca over a broad N fertility range. In the high to moderate N fertility range, leaf lifespan and rN increased with decreasing N addition rate, and thus the MRTL increased. However, in the moderate to low N fertility range, leaf lifespan increased but rN decreased significantly, so MRTL decreased. The decrease in rN occurred because the senesced leaf N concentration was almost constant at the lower limit while the green leaf N concentration decreased in this range. The hump-shaped quadratic responses of MRTL and rN along the N fertility gradient suggest that incorrect conclusions about the response of these traits to N fertility variation may be drawn from experiments that include only a few fertility levels, and N recycling within leaf canopy alone cannot achieve efficient N use in infertile environments.

Species identification through deep learning and geometrical morphology in oaks (Quercus spp.): Pros and cons.

Plant phenotypic characteristics, especially leaf morphology of leaves, are an important indicator for species identification. However, leaf shape can be extraordinarily complex in some species, such as oaks. The great variation in leaf morphology and difficulty of species identification in oaks have attracted the attention of scientists since Charles Darwin. Recent advances in discrimination technology have provided opportunities to understand leaf morphology variation in oaks. Here, we aimed to compare the accuracy and efficiency of species identification in two closely related deciduous oaks by geometric morphometric method (GMM) and deep learning using preliminary identification of simple sequence repeats (nSSRs) as a prior. A total of 538 Asian deciduous oak trees, 16 Q. aliena and 23 Q. dentata populations, were firstly assigned by nSSRs Bayesian clustering analysis to one of the two species or admixture and this grouping served as a priori identification of these trees. Then we analyzed the shapes of 2328 leaves from the 538 trees in terms of 13 characters (landmarks) by GMM. Finally, we trained and classified 2221 leaf-scanned images with Xception architecture using deep learning. The two species can be identified by GMM and deep learning using genetic analysis as a priori. Deep learning is the most cost-efficient method in terms of time-consuming, while GMM can confirm the admixture individuals' leaf shape. These various methods provide high classification accuracy, highlight the application in plant classification research, and are ready to be applied to other morphology analysis.

Will “Tall Oaks from Little Acorns Grow”? White Oak (Quercus alba) Biology in the Anthropocene

Quercus alba L., also known as white oak, eastern white oak, or American white oak, is a quintessential North American species within the white oak section (Quercus) of the genus Quercus, subgenus Quercus. This species plays a vital role as a keystone species in eastern North American forests and plays a significant role in local and regional economies. As a long-lived woody perennial covering an extensive natural range, Q. alba’s biology is shaped by a myriad of adaptations accumulated throughout its natural history. Populations of Q. alba are crucial repositories of genetic, genomic, and evolutionary insights, capturing the essence of successful historical adaptations and ongoing responses to contemporary environmental challenges in the Anthropocene. This intersection offers an exceptional opportunity to integrate genomic knowledge with the discovery of climate-relevant traits, advancing tree improvement, forest ecology, and forest management strategies. This review provides a comprehensive examination of the current understanding of Q. alba’s biology, considering past, present, and future research perspectives. It encompasses aspects such as distribution, phylogeny, population structure, key adaptive traits to cyclical environmental conditions (including water use, reproduction, propagation, and growth), as well as the species’ resilience to biotic and abiotic stressors. Additionally, this review highlights the state-of-the-art research resources available for the Quercus genus, including Q. alba, showcasing developments in genetics, genomics, biotechnology, and phenomics tools. This overview lays the groundwork for exploring and elucidating the principles of longevity in plants, positioning Q. alba as an emerging model tree species, ideally suited for investigating the biology of climate-relevant traits.