Application Of Amendments Research Articles

Long-term Application of Agricultural Amendments Regulate Hydroxyl Radicals Production during Oxygenation of Paddy Soils.

Hydroxyl radicals (•OH) play a significant role in contaminant transformation and element cycling during redox fluctuations in paddy soil. However, these important processes might be affected by widely used agricultural amendments, such as urea, pig manure, and biochar, which have rarely been explored, especially regarding their impact on soil aggregates and associated biogeochemical processes. Herein, based on five years of fertilization experiments in the field, we found that agricultural amendments, especially coapplication of fertilizers and biochar, significantly increased soil organic carbon contents and the abundances of iron (Fe)-reducing bacteria. They also substantially altered the fraction of soil aggregates, which consequently enhanced the electron-donating capacity and the formation of active Fe(II) species (i.e., 0.5 M HCl-Fe(II)) in soil aggregates (0-2 mm), especially in small aggregates (0-3 μm). The highest contents of active Fe(II) species in small aggregates were mainly responsible for the highest •OH production (increased by 1.7-2.4-fold) and naphthalene attenuation in paddy soil with coapplication of fertilizers and biochar. Overall, this study offers new insights into the effects of agricultural amendments on regulating •OH formation in paddy soil and proposes feasible strategies for soil remediation in agricultural fields, especially in soils with frequent occurrences of redox fluctuations.

Enhancing wheat productivity, quality and nutrient uptake through application of phosphate rich organic manure and soil amendments under Typic Hapludalfs

Enhancing wheat productivity, quality and nutrient uptake through application of phosphate rich organic manure and soil amendments under Typic Hapludalfs

Efficacy evaluation of biochar and activated carbon as carriers of bacterial inoculants in the remediation of multi-metal polluted soil

ABSTRACT Application of appropriate organic amendments as the carriers of bacterial consortium may improve the remediation efficiency of HMs-polluted soil. A greenhouse experiment was designed and carried out to investigate the capability of biochar, and activated carbon prepared from ostrich manure and almond husk as the carriers of bacterial inoculants in the phytoremediation of a calcareous soil polluted with Pb, Ni, Cd and Zn by maize. Results showed that the application of biochar and activated carbon prepared from ostrich manure increased root (78–129%) and shoot (72.3–272%) dry weight, as compared to the control. The values of metal accumulation in both maize root and shoot were in the order of Cd>Zn>Ni>Pb. While biochar and activated carbon prepared from ostrich manure significantly increased both root and shoot metals uptake, those prepared from almond husk drastically decreased the uptake of some metals. The foremost mechanism involved in the phytoremediation of Cd, Ni and Pb was phytostabilization while that of Zn was due to the phytoextraction. Results of the present study demonstrated the effectiveness of ostrich manure-derived biochar and activated carbon as an efficient treatment in the phytoremediation of multi-metal-polluted soils and the mitigation of HMs phytotoxicity.

The effect of pruned tea leave biochar and cattle manure for soil acidification

AbstractSoil acidification in tea gardens has become an increasingly serious problem. In this study, we conducted an outdoor pot experiment to investigate the effects of pruned tea leaf biochar and cattle manure on soil acidification. We observed that amendments effectively increased soil pHBC, which increased 12.43%, 3.24%, 8.38%, 5.68% and 11.35% for BC, M, BCM1, BCM2 and BCM3. Exchangeable K+, Na+, Ca2+ and Mg2+ concentrations increased after application of the amendments. Furthermore, the total exchangeable acidity concentration (EA) and the exchangeable Al3+ and H+ concentration (Al3+ and H+) decreased after application of amendments. In addition, pHBC showed a strong positive correlation with K+, Na+, Ca2+, Mg3+ and cation exchange capacity and strong negative correlations with EA and Al3+. Moreover, EA and K+ were the primary factors influencing pHBC. The relationships among pHBC, EA and K+ can be expressed as a power function.

Zinc phytotoxicity and cationic micronutrients contents in quinoa as influenced by high Zn levels and soil amendments

Contamination of soils with heavy metals (HMs) is a serious concern due to the non-biodegradability and accumulation of these metals in living organism. The aim of this study was to evaluate the effectiveness of fish scale powder, chitin, and chitosan in reducing zinc (Zn) accumulation of quinoa grown on Zn-polluted soil. A 3*4 factorial greenhouse trial was conducted with three levels of Zn contamination (0, 100, and 200 mg kg−1) and four types of amendment (control, and 0.15% of each fish scale powder, chitin, and chitosan). The quinoa seeds were sown in soil treated with Zn contamination and amendments. Results showed that application of amendments especially chitosan increased shoot and root dry weight and greenness index of quinoa leaf. While high soil Zn levels decreased cationic micronutrients uptake in quinoa shoot, chitosan addition desirably enhanced cationic micronutrients uptake in quinoa shoot. Overall, amendment addition significantly decreased Zn concentration and uptake in plant; the highest decrease was observed following the application of chitosan. Although the application of high Zn levels significantly increased the values of phytoextraction efficiency, application of chitin and chitosan significantly decreased it. The value of translocation factor was lower than one, demonstrating that a large amount of Zn was stabilized in the root tissues, while a small amount of this metal was transferred to aboveground parts of quinoa. Results of this study revealed that fish scales derivatives were effective in preventing Zn toxicity and at the same time increasing cationic micronutrients contents of quinoa grown on HMs-polluted soil.

Organic matter, carbon, and nitrogen relationships of regreened forest soils in an industrially impacted landscape

Context Soil organic matter (SOM) is largely composed of carbon (C) and nitrogen (N), the proportions of which often change with soil depth. The relationships between SOM, C, and N in forest soils can be greatly altered in degraded landscapes and understanding these relationships is integral for successful forest restoration planning. Aims The current study investigated SOM, C, and N relationships in highly degraded forest soils by depth following regreening (one-time application of soil amendments and afforestation). Additionally, the use of standard C:OM ratios (which are commonly used to estimate soil C) were assessed. Methods The SOM, C, and N were measured at five different depths, at nine sites, ranging in time since regreening treatment applications across one of the world’s largest regreening programmes in the City of Greater Sudbury, Canada. Key results The C:OM and C:N ratios decreased with soil depth while N:OM increased. The C and N were significantly correlated with SOM at all depths (excluding the L horizon). The C:OM ratio was lower than standard values and did not change between 16 and 41 years since the application of 10 Mg ha−1 of dolomitic limestone. Conclusions Despite massive soil degradation, SOM, C, and N relationships over soil depth at the regreening sites are consistent with unimpacted forest soils. Applying commonly used C:OM ratios drastically overestimated soil C pools, especially at lower depths. Implications Even in the most degraded landscapes, restoration can improve soil properties. Standard C:OM ratios should be used with caution.

Application of New Polymer Soil Amendment in Ecological Restoration of High-Steep Rocky Slope in Seasonally Frozen Soil Areas.

In seasonally frozen soil areas, high-steep rocky slopes resulting from open-pit mining and slope cutting during road construction undergo slow natural restoration, making ecological restoration generally challenging. In order to improve the problems of external soil attachment and long-term vegetation growth in the ecological restoration of high-steep rocky slopes in seasonally frozen areas, this study conducted a series of experiments through the combined application of polyacrylamide (PAM) and carboxymethyl cellulose (CMC) to assess the effects of soil amendments on soil shear strength, water stability, freeze-thaw resistance, erosion resistance, and vegetation growth. This study showed that the addition of PAM-CMC significantly increased the shear resistance and cohesion of the soil, as well as improving the water stability, freeze-thaw resistance, and erosion resistance, but the internal friction angle of the soil was not significantly increased after reaching a certain content. Moderate amounts of PAM-CMC can extend the survival of vegetation, but overuse may cause soil hardening and inhibit vegetation growth by limiting air permeability. It was observed by a scanning electron microscope (SEM) that the gel membrane formed by PAM-CMC helped to "bridge" and bind the soil particles. After discussion and analysis, the optimum application rate of PAM-CMC was 3%, which not only improved the soil structure but also ensured the growth of vegetation in the later stage under the optimum application rate. Field application studies have shown that 3% PAM-CMC-amended soil stably attaches to high-steep rocky slopes, with stable vegetation growth, and continues to grow after five months of freeze-thaw action, with no need for manual maintenance after one year.

Enhanced Phytoremediation of Heavy Metals By Soil-applied Organic And Inorganic Amendments: Heavy Metal Phytoavailability, Accumulation, And Metal Recovery

ABSTRACT Phytoremediation, an eco-friendly and cost-effective technique for remediating heavy metal-contaminated soils, can be significantly enhanced through the application of soil amendments. This study investigates the impact of Foliar spraying of both organic (fulvic acid, auxin, Compost, and coconut coir) and inorganic (sulfur) amendments on the phytoavailability, accumulation, and recovery of heavy metals: Arsenic-As, Mercury-Hg, Cadmium-Cd and Lead-Pb in contaminated soils. Field experiment was conducted using hyperaccumulator plant species; T. capensis and H. psittacorum with varying amendment treatments to assess their effectiveness in improving phytoremediation outcomes. The results demonstrate that specific amendments significantly increase heavy metal uptake and translocation within plants by altering soil pH, cation exchange capacity, and microbial activity, thus enhancing metal solubility and bioavailability. Furthermore, the study evaluates the potential of these amendments to not only facilitate metal recovery but also to restore soil health and fertility. The findings underscore the potential of tailored amendment strategies in optimizing phytoremediation processes, offering a sustainable solution for heavy metal remediation in polluted environments.

Application of different organic amendments influences the different forms of sulphur in the soil of pea – onion – cauliflower cropping system

A study was conducted in sandy clay loam soils in a subtropical zone of Bihar to evaluate the effect of frequent application of organic amendments on sulphur fractions. Different organic amendments, including farmyard manure (FYM), vermicompost, azotobacter, phosphate solubilizing bacteria (PSB), panchagawya, and neem cake, were applied through nine treatments that resulted in a significant increase of water-soluble S, available S, heat-soluble S, adsorbed S, and organic S in organic treatment compared to the recommended dose of fertilizer (RDF) and control treatment. The maximum increment was observed in the treatment where the recommended dose of nitrogen was replaced by 75% recommended dose of nitrogen substituted farmyard manure + 25% recommended dose of nitrogen (vermicompost) along with azotobacter + PSB + one foliar spray of panchagawya. The total S content varied widely from 382 to 736 mg kg-1. Increment in all the forms of sulphur is observed as a result of the application of different organic nutrient sources. All the forms of sulphur share a mutual positive and significant correlation with each other. Regression analysis suggested that the availability of sulphur was dominated by organic sulphur, which alone can explain 97.8% of the variation in availability of available sulphur in soil.

Applying conditioners to address the issues of carbon loss and cadmium pollution in the karst paddy-oil crop system

The fragile ecosystem and unique parent material in the karst region lead to severe carbon loss and cadmium pollution. Additionally, the rice-oilseed rotation system exacerbates the accumulation of cadmium in crops. Therefore, it is necessary to find a sustainable land use approach that ensures crop quality while promoting soil carbon sequestration. This article provides an overview of cutting-edge technologies to enhance soil carbon sequestration capacity, and it discovers that the application of amendments has a significant effect on reducing cadmium content in crops and improving soil organic carbon levels. Through experimental analysis, The results indicate that the conditioner in group T4 (Humic acids) can effectively passivate cadmium elements in the soil, reducing the cadmium content in rice and rapeseed tissues. Overall, our research indicates that humic acid amendments not only enhance soil carbon sequestration capacity but also significantly reduce cadmium content in crops and increase crop yields. These findings highlight the significant value of humic acid amendments in the rice-oilseed rotation system in the karst region, providing scientific evidence and theoretical support for promoting sustainable development in the area.

Practice of Improving Saline–Alkali Soil with Bio-Humic Acid

The improvement and exploitation of saline–alkali soil is a hot topic worldwide. Analysis of soil aggregate structure and water and of the salt transport law, a new technology that uses humic acid as a saline–alkali soil amendment has been proposed. These advancements may effectively improve the soil aggregate structure of saline–alkali soil at the source. In this study, biological pyrolysis liquid was modified to produce soil modifier for saline–alkali land, and this soil modifier was used in a rice planting experiment. The results show that the application of a bio-humic acid soil amendment in the amount of 3000 kg/ha and its combination with humic acid fertilizer produced rice yields as high as 9750 kg/ha. At the same time, the pH of the soil was reduced from 10.81 to 8.95 (with bio-humic acid soil amendment content of 3000 kg/ha), and the soil organic matter demonstrated a trend of increasing with an increased amount of bio-humic acid soil amendment. The content of calcium, magnesium, and trace elements in the soil were clearly increased with the use of the amended soil in comparison with the normal group. With this amendment, saline–alkali land could be transformed and planted in the same year, increasing the potential yearly income for the land. This may be efficient and environmentally friendly, encouraging the agricultural circular economy.

Integrating waste valorization and symbiotic microorganisms for sustainable bioremediation of metal(loid)-polluted soils

Remediation strategies for metal(loid)-polluted soils vary among the wide range of approaches, including physical, chemical, and biological remediation, or combinations of these. In this study, we assessed the effectiveness of a set of soil remediation treatments based on the combined application of inorganic (marble sludge) and organic amendments (vermicompost, and dry olive residue [DOR] biotransformed by the saprobic fungi Coriolopsis rigida and Coprinellus radians) and inoculation with arbuscular mycorrhizal fungi (AMFs) (Rhizophagus irregularis and Rhizoglomus custos). The treatments were applied under greenhouse conditions to soil residually polluted by potentially toxic elements (PTEs) (Pb, As, Zn, Cu, Cd, and Sb), and wheat was grown in the amended soils to test the effectiveness of the treatments in reducing soil toxicity and improving soil conditions and plant performance. Therefore, we evaluated the influence of the treatments on the main soil properties and microbial activities, as well as on PTE availability and bioaccumulation in wheat plants. Overall, the results showed a positive influence of all treatments on the main soil properties. Treatments consisting of a combination of marble and organic amendments, especially biotransformed DOR amendments, showed the greatest effectiveness in improving the soil biological status, promoting plant growth and survival, and reducing PTE availability and plant uptake. Furthermore, AMF inoculation further enhanced the efficacy of DOR amendments by promoting the immobilization of PTEs in soil and stimulating the phytostabilization mechanisms induced by AMFs, thus playing an important bioprotective role in plants. Therefore, our results highlight that biotransformed DOR may represent an efficient product for use as a soil organic amendment when remediating metal(loid)-polluted soils, and that its application in combination with AMFs may represent a promising sustainable bioremediation strategy for recovering soil functions and reducing toxicity in polluted areas.

Incorporation of compost and biochar enhances yield and medicinal compounds in seeds of water-stressed Trigonella foenum-graecum L. plants cultivated in saline calcareous soils

BackgroundThe combination of compost and biochar (CB) plays an important role in soil restoration and mitigation strategies against drought stress in plants. In the current study, the impact of CB was determined on the characteristics of saline calcareous soil and the productivity of fenugreek (Trigonella foenum-graecum L.) plants. The field trials examined CB rates (CB0, CB10 and CB20 corresponding to 0, 10, and 20 t ha‒1, respectively) under deficit irrigation [DI0%, DI20%, and DI40% receiving 100, 80, and 60% crop evapotranspiration (ETc), respectively] conditions on growth, seed yield (SY), quality, and water productivity (WP) of fenugreek grown in saline calcareous soils.ResultsIn general, DI negatively affected the morpho-physio-biochemical responses in plants cultivated in saline calcareous soils. However, amendments of CB10 or CB20 improved soil structure under DI conditions. This was evidenced by the decreased pH, electrical conductivity of soil extract (ECe), and bulk density but increased organic matter, macronutrient (N, P, and K) availability, water retention, and total porosity; thus, maintaining better water and nutritional status. These soil modifications improved chlorophyll, tissue water contents, cell membrane stability, photosystem II photochemical efficiency, photosynthetic performance, and nutritional homeostasis of drought-stressed plants. This was also supported by increased osmolytes, non-enzymatic, and enzymatic activities under DI conditions. Regardless of DI regimes, SY was significantly (P ≤ 0.05) improved by 40.0 and 102.5% when plants were treated with CB10 and CB20, respectively, as similarly observed for seed alkaloids (87.0, and 39.1%), trigonelline content (43.8, and 16.7%) and WP (40.9, and 104.5%) over unamended control plants.ConclusionsOverall, the application of organic amendments of CB can be a promising sustainable solution for improving saline calcareous soil properties, mitigating the negative effects of DI stress, and enhancing crop productivity in arid and semi-arid agro-climates.

Sugarcane bagasse biochar boosts maize growth and yield in salt-affected soil by improving soil enzymatic activities

Salinization is a leading threat to soil degradation and sustainable crop production. The application of organic amendments could improve crop growth in saline soil. Thus, we assessed the impact of sugarcane bagasse (SB) and its biochar (SBB) on soil enzymatic activity and growth response of maize crop at three various percentages (0.5%, 1%, and 2% of soil) under three salinity levels (1.66, 4, and 8 dS m−1). Each treatment was replicated three times in a completely randomized block design with factorial settings. The results showed that SB and SBB can restore the impact of salinization, but the SBB at the 2% addition rate revealed promising results compared to SB. The 2% SBB significantly enhanced shoot length (23.4%, 26.1%, and 41.8%), root length (16.8%, 20.8%, and 39.0%), grain yield (17.6%, 25.1%, and 392.2%), relative water contents (11.2%, 13.1%, and 19.2%), protein (17.2%, 19.6%, and 34.9%), and carotenoid (16.3, 30.3, and 49.9%) under different salinity levels (1.66, 4, and 8 dS m−1, respectively). The 2% SBB substantially drop the Na+ in maize root (28.3%, 29.9%, and 22.4%) and shoot (36.1%, 37.2%, and 38.5%) at 1.66, 4, and 8 dS m−1. Moreover, 2% SBB is the best treatment to boost the urease by 110.1%, 71.7%, and 91.2%, alkaline phosphatase by 28.8%, 38.8%, and 57.6%, and acid phosphatase by 48.4%, 80.1%, and 68.2% than control treatment under 1.66, 4 and 8 dS m−1, respectively. Pearson analysis showed that all the growth and yield parameters were positively associated with the soil enzymatic activities and negatively correlated with electrolyte leakage and sodium. The structural equational model (SEM) showed that the different application percentage of amendments significantly influences the growth and physiological parameters at all salinity levels. SEM explained the 81%, 92%, and 95% changes in maize yield under 1.66, 4, and 8 dS m−1, respectively. So, it is concluded that the 2% SBB could be an efficient approach to enhance the maize yield by ameliorating the noxious effect of degraded saline soil.

Response of ectomycorrhizal fungi to full and selective removal of an invasive tree in riparian woodland

The Brazilian pepper tree (Schinus terebinthifolius) is an invasive species that requires significant disturbance to eradicate. Previous studies have identified associations between the Brazilian pepper tree and ectomycorrhizal fungi (EMF). However, limited research has explored the connection between disturbance from removal and the effect on EMF. This study investigated the sensitivity of EMF and the broader fungal community to the full and selective removal of the Brazilian pepper tree. During the selective removal of Brazilian pepper tree, we examined the mycorrhizal community of the Arroyo willow (Salix lasiolepis) to assess the influence of the restoration disturbance on native species. We used ITS2 sequencing to identify the EMF present during the restoration. Our expectation was that both removal efforts would reduce the presence of EMF. Contrary to our predictions, full removal increased EMF richness and relative abundance in the soil. As anticipated, selective removal reduced the richness and relative abundance of EMF associated with soil. Selective removal led to a decrease in the richness of EMF in arroyo willow roots with no effect on relative abundance. Moreover, fungal community composition in soil and roots shifted significantly during selective and full removal. However, the community composition of EMF, specifically, remained constant across treatment types. During full removal efforts, the application of organic soil amendments may have contributed to the increase in the diversity and relative abundance of EMF. Selective removal will require additional measures, such as soil amendments, to curtail the loss of EMF.

Accumulation and Translocation of Heavy Metals in Hibiscus cannabinus Grown in Tannery Sludge Amended Soil

Digested sludge wasted by tanneries is rich in nutrients and trace elements however, the presence of toxic metals restricts their use in agriculture. The present study explores the possible application of tannery sludge amendment for the cultivation of an energy crop, Hibiscus cannabinus. The toxicity of various sludge amendments (25, 50, 75, and 100%, w/w) was examined during early seedling growth, followed by metal accumulation potential by performing pot experiments. Chemical characterization revealed the presence of Cr (709.6), Cu (366.43), Ni (74.6), Cd (132.71), Pb (454.8) μg.g-1 in tannery sludge beside N (2.1%), P 3.8 & K 316.96 (kg.hec-1.) respectively. Germination of H. cannabinus exposed to sludge extracts ranged between 80 to 95%; Relative seed germination, 81.33 to 84.43%. Relative root growth, 0.9 to 1.16 cm; and germination index, 95 to 110%. It was found that sludge extracts have not caused adverse effects on seed germination and early seedling growth. Heavy metal accumulation was observed as follows: Ni (3.37, 2.38, 1.46 & 0.90 mg.kg-1) > Pb (10.59, 10.15, 5.26, & 2.84 mg.kg-1) > Cu (2.34, 2.24, 0.97 & 0.24 mg.kg-1) > Cd (2.31, 1.19, 1.33 & 1.12 mg.kg-1) > Cr (1458, 1136.12, 601.73 & 211.6 mg.kg-1) in 100, 75, 50, & 25% sludge amended soil, respectively. The bio-concentration pattern of metals was found to be in the order of root > leaf > stem. The findings of the present study give direction for the eco-friendly and cost-effective management of tannery sludge. Further, H. cannabinus can be used for the restoration of metal-contaminated agricultural land, however, results need to be corroborated with field trials.

Effectiveness of nanomaterials and their counterparts in improving rice growth and yield under arsenic contamination.

Arsenic (As) pollution in rice (Oryza sativa L.), a staple food for over 3.5 billion people, is a global problem. Mixed effects of Zn, Cu, and Si amendments on plant growth and yield, including in the presence of As pollution have been reported in previous studies. To better investigate the effectiveness of these amendments on rice growth, yield, and As accumulation, we conducted a rice greenhouse experiment with 11 treatments, including control pots with and without As contamination and pots with amendments of ZnO, CuO, and SiO2 nanoparticles (ZnO NPs, CuO NPs, and SiO2 NPs), their ionic counterparts (ZnSO4, CuSO4, and Na2SiO3), and bulk particles (ZnO BPs, CuO BPs, and SiO2 BPs). Compared with the background soil, the treatment of adding As decreased rice plant height, panicle number, and grain yield by 16.5%, 50%, and 85.7%, respectively, but significantly increased the As accumulation in milled rice grains by 3.2 times. Under As contamination, the application of Zn amendments increased rice grain yield by 4.6-7.3 times; among the three Zn amendments, ZnSO4 performed best by fully recovering grain yield to the background level and significantly reducing grain AsIII/total As ratio by 46.9%. Under As contamination, the application of Cu amendments increased grain yield by 3.8-5.6 times; all three Cu amendments significantly reduced grain AsIII/total As ratio by 20.2-65.6%. The results reveal that Zn and Cu amendments could promote rice yield and prevent As accumulation in rice grains under As contamination. Despite the observed reduction in As toxicity by the tested NPs, they do not offer more advantages over their ionic counterparts and bulk particles in promoting rice growth under As contamination. Future field research using a broader range of rice varieties, investigating various As concentrations, and encompassing diverse climate conditions will be necessary to validate our findings in achieving more extensive understanding of effective management of arsenic contaminated rice field.

Organic amendments improved soil properties and native plants’ performance in an Australian degraded land

Context Land degradation poses a substantial threat to both the sustainable environment and human health. Efforts towards rehabilitation and remediation often require addition of soil amendments and careful selection of plant species. Aims We assessed the effect of recycled organic amendments on improvement of soil physicochemical properties and performance of native plant species in an Australian degraded soil. Methods A glasshouse pot experiment investigated the effects of compost (CO), biochar (BC), and compost-biochar (COBC) mixture on performance of three native Australian plant species (Eucalyptus tereticornis (EU), Acacia leiocalyx (AC), and Banksia integrifolia (BA)) in a degraded soil. Key results Application of CO, BC, and COBC organic amendments increased soil dissolved organic carbon and microbial biomass carbon contents compared to the control treatment. COBC amendment increased nutrient retention and reduced CO2 emissions compared to CO amendment. BC amendment also resulted in low CO2 emissions similar to the control treatment, where no significant differences were observed. AC outperformed the EU and BA species in biomass production due to its leguminous nature, with amendment application had an insignificant effect on AC performance. Within the EU treatments, the COBC:EU demonstrated the highest biomass production, followed by CO:EU, BC:EU, and CK:EU, respectively. Conclusion All amendments exhibited overall improvements in soil and plant parameters, with more significant outcomes observed with COBC application. However, the observed improvements from biochar application were minimal in this short-term experiment, which may not have allowed for the manifestation of long-term benefits. Implications Further research is warranted to investigate the effects of compost and biochar amendments on diverse soil types and native plant species.

A Comprehensive Perception of Biological Control Potential of Endophytes and Quality Refinement of Lagenaria siceraria.

Agriculture and livestock management practices known as organic farming rely more on internal processes than external inputs. Natural environments depend heavily on diversity, and organic farming incorporates both the stated purpose of fostering diversity as well as the use of diversity as a management tool. A more complete understanding of agriculture in terms of agro-ecology has begun to be questioned by the traditional reductionist approach to the study of agriculture. Therefore it is necessary to be aware more about the significance of microbes in processes including soil growth, plant nourishment, and the eradication of plant disease, pest, and weeds. In this study, fluorescent Pseudomonas strain (EFP56) and Trichoderma harzianum were studied for antifungal and antibacterial activity against four common root rot fungi and four common laboratory bacteria in vitro experiments. Furthermore, soil-borne disease surveillance and nutritional quality of Lagenaria siceraria, fluorescent Pseudomonas strain (EFP56) and Trichoderma harzianum were combined with neem cake and cotton cake to check their efficacy. Through the application of organic soil amendments in combination with biocontrol agents improved the quality of vegetables and their nutritional value by raising their polyphenol, carbohydrate, and protein content as well as enhancing antioxidant scavenging status. The experiments were conducted in pots and in fields to confirm their efficacy rate. The final outcomes also revealed greater induction of defense system, disease lessening and enriched fruit quality. Consortium of neem cake and cotton cake with bio-stimulants can regulate biotic as well as abiotic stress.

Bacterial Endophytes and Their Contributions to Alleviating Drought and Salinity Stresses in Wheat: A Systematic Review of Physiological Mechanisms

Drought and salinity stresses significantly threaten global wheat productivity, limiting growth and reducing yields, thus endangering food security worldwide. These stresses disrupt physiological processes, impair photosynthesis, and hinder optimal growth and yield by diminishing water uptake, causing osmotic stress, ion toxicity, and oxidative stress. In response, various mitigation strategies have been explored, including breeding for stress-tolerant cultivars, improved irrigation techniques, and the application of exogenous osmoprotectants and soil amendments. Among these strategies, the emergence of rhizospheric and endophytic growth-promoting microorganisms has attracted significant attention. Therefore, a systematic review was undertaken to illustrate the role of endophytic bacteria in enhancing wheat tolerance to drought and salinity stresses. This review analyzes physiological mechanisms and research trends, identifies gaps, and discusses implications for sustainable agriculture. An analysis of the literature related to endophytic bacteria in wheat was conducted using databases of major publishers from 2004 to 2023. The review explores their mechanisms, such as phytohormone production and stress-responsive gene induction, emphasizing their contribution to plant growth and stress resilience. The current research trends indicate a growing interest in utilizing endophytic bacteria to mitigate these stresses in wheat cultivation, with studies focusing on understanding their physiological responses and interactions with wheat plants. Future research should concentrate on elucidating the role of endophytic bacteria in enhancing host plant tolerance to multiple stressors, as well as aspects like endophytic mechanism of action , endophytic lifestyle, and transmission pathways. Overall, endophytic bacteria offer promising avenues for sustainable agricultural practices, aiding in crop resilience and food security amid environmental challenges.