Amblyomma Geoemydae Research Articles

Observation of the Asian Turtle Tick Amblyomma geoemydae Cantor, 1847 (Acari: Ixodidae) on the Asian Leaf Turtle Cyclemys dentata Gray, 1831 in Coron Island, Philippines

The Asian leaf turtle (Cyclemys dentata) is a freshwater turtle species found in select Southeast Asian countries, including the Philippines. Our understanding of C. dentata’s ecology in the Philippines is not yet fully understood and documented, and this is derived only from a few studies. In this paper, we present our single-event observation of an ectoparasite interaction between C. dentata and the Asian turtle tick (Amblyomma geoemydae) from Sitio Malbato, Barangay Bintuan Coron, Palawan. Our discovery serves as the second documented instance of this ectoparasite on C. dentata in the Philippines, hence extending its known distribution outside mainland Palawan. This finding contributes to the limited understanding of C. dentata's ecology and underscores the need for further field-based observations, which are crucial for their research and conservation.

Molecular characterization of Amblyomma geoemydae using CO1 mitochondrial gene to validate phenotypic taxonomical evaluation.

Animal ectoparasites are linked to the spread of serious medical and veterinary important pathogens. Our research intends to close the knowledge gap concerning the numerous ectoparasites that inhabit animals in Wayanad. Ectoparasites in animals brought to the veterinary dispensaries in Wayanad were retrieved and identified morphologically and molecularly. Using a high-quality stereomicroscope, the taxonomic features of the four following species were examined and identified: Haemaphysalis bispinosa, Rhipicephalus annulatus, R. microplus, and Amblyomma geoemydae. The important disease vector A. geoemydae was reported for the first time in Kerala. The important phenotypic characters of the highlighted species A. geoemydae are the edge of the basis capituli is circular without cornua, and the hypostomal dental formula is 2/2. The taxonomically identified four species were subjected to CO1 gene sequence analysis. The evolutionary relationship was inspected through the neighbour-joining method, and the phylogenetic tree was built through the Maximum Likelihood method. The present study has also estimated the diversity index of R. microplus, R. annulatus, H. bispinosa, and A. geoemydae. Among them, R. microplus 0.36638 have reported with the maximum diversity index score. The significance of the study is the presence of Lyme disease vector A. geoemydae, in the Wayanad District of Kerala, and it is the first report of the species from where an outbreak of Lyme disease occurred in 2013.

The Asian turtle tick Amblyomma geoemydae Cantor, 1847 (Acari: Ixodidae) in the Philippines: first confirmed local host and locality with a complete host index

Amblyomma geoemydae is an oriental tick species primarily associated with turtles and tortoises but also sometimes reported from other vertebrates, including humans. Although A. geoemydae was first reported from the Philippines more than 70 years ago, it has not been reported since, and no local distributional or host data have been published. Herein, we present the first definitive locality report and host record for A. geoemydae in the Philippines. A complete checklist of the known host species from which A. geoemydae has been collected is also presented.

First Record of the Tortoise Tick, Amblyomma geoemydae (Cantor, 1847) (Acari: Ixodidae) Parasitizing a Tree Shrew, Tupaia glis (Scandentia: Tupaiidae) in West Malaysia.

The tick genus Amblyomma Koch, 1844 (Acari: Ixodidae) has received little attention in Malaysia; therefore, its associated hosts and distribution records are poorly known. In this study, we collected six Amblyomma sp. individuals (two larvae and four adults) that infested a common treeshrew, Tupaia glis (Diard, 1820) (Scandentia: Tupaiidae) caught in a recreational area in Sungai Lembing, Pahang (West Malaysia). The adult female ticks were morphologically identified according to taxonomic keys prior to molecular identification using cytochrome oxidase subunit I (COI) and 16S rDNA genes. The ticks were genetically verified as Amblyomma geoemydae (Cantor, 1847) with 98%-99% similarity to the available GenBank sequences. Neighbor-joining (NJ) trees indicated that A. geoemydae was clearly distinguished from other Amblyomma ticks and this was supported with high bootstrap values. This paper is the first to report A. geoemydae ticks infesting T. glis and provides a new tick-host record from West Malaysia. This information is significant for further investigation, specifically on this tick species as potential vector of tick-borne disease (TBD) agents.

Molecular and morphological identification of a reptile-associated tick, Amblyomma geoemydae (Acari: Ixodidae), infesting wild yellow-margined box turtles (Cuora flavomarginata) in northern Taiwan

The genetic identity and morphological features of a reptile-associated tick, Amblyomma geoemydae, were examined for the first time in Taiwan. The key morphological characters of male and female Am. geoemydae were photographed using a stereo microscope. The genetic identity was analyzed by comparing the 16S mitochondrial DNA gene sequences obtained from 11 Taiwan Am. geoemydae ticks with other Amblyomma species and three Ixodes species as outgroups. All the Taiwan specimens were genetically affiliated to a monophyletic group of Am. geoemydae and could be discriminated from other Amblyomma species. Our results provide the first distinguished colour figures and first genetic identification of adult Am. geoemydae ticks infesting turtles in Taiwan. The host range and vectorial capacity of Am. geoemydae for various tick-borne pathogens need to be further clarified in Taiwan.

First molecular detection of Hemolivia and Hepatozoon parasites in reptile-associated ticks on Iriomote Island, Japan.

Hepatozoon and Hemolivia are members of the haemogregarines and are reported in reptiles and reptile-associated ticks. However, no studies have reported on Hepatozoon and Hemolivia in Japanese reptile-associated ticks. This study aimed to molecularly identify and to characterize Hepatozoon and Hemolivia in Japanese reptile-associated ticks, Amblyomma geoemydae (Cantor, 1847) and Amblyomma nitidum (Hirst & Hirst, 1910). A total of 41 and 75 DNA samples from A. geoemydae and A. nitidum ticks, respectively, were used for screening of Hepatozoon and Hemolivia with polymerase chain reaction targeting 18S rDNA. As a result, Hemolivia and Hepatozoon were detected in two A. geoemydae and one A. nitidum, respectively. The sequences of Hemolivia spp. showed a 99.5% (1,050/1,055bp) identity with Hemolivia parvula (KR069083), and the Hemolivia spp. were located in the same clade as H. parvula in the phylogenetic tree. The sequences of Hepatozoon sp. showed a 98.4% (1,521/1,545bp) identity with Hepatozoon colubri (MN723844), and the Hepatozoon sp. was distinct from validated Hepatozoon species in the tree. Our findings highlight the first molecular record of Hemolivia in Japan and present the first detection of Hepatozoon in A. nitidum. Further investigations on these tick-borne protozoa are required to understand their life cycle and pathogenicity.

Detection of Novel Spotted Fever Group Rickettsiae (Rickettsiales: Rickettsiaceae) in Ticks (Acari: Ixodidae) in Southwestern China.

Spotted fever group rickettsiae, mainly maintained and transmitted by ticks, are important etiological agents of (re)emerging zoonotic diseases worldwide. It is of great significance to investigate spotted fever group rickettsiae in ticks in different areas for the prevention and control of rickettsioses. In this study, a total of 305 ticks were collected from wild and domestic animals in Chongqing, Guizhou, Yunnan, and Guangxi provinces of southwestern China during 2017-2019 and examined for the presence of spotted fever group rickettsiae by PCR with primers targeting the partial gltA, ompA, rrs, and htrA genes. Results showed that two spotted fever group rickettsiae species, including the pathogenic Candidatus Rickettsia jingxinensis (Rickettsiales: Rickettsiaceae) and a potential novel species Rickettsia sp. sw (Rickettsiales: Rickettsiaceae), were identified. The Ca. R. jingxinensis sequences were recovered from Rhipicephalus microplus (Ixodida: Ixodidae) and Haemaphysalis longicornis (Ixodida: Ixodidae) ticks and phylogenetically clustered with previous Ca. R. jingxinensis, Ca. R. longicornii (Rickettsiales: Rickettsiaceae), and Rickettsia sp. XY118 (Rickettsiales: Rickettsiaceae) strains. Rickettsia sp. sw was detected in Amblyomma geoemydae (Ixodida: Ixodidae) and Rh. microplus. Interestingly, as far as we know, this was the first report of Rickettsia (Rickettsiales: Rickettsiaceae) in A. geoemydae. Phylogenetic analyses indicated that this potential novel species was closely related to R. aeschlimannii (Rickettsiales: Rickettsiaceae) with gltA and ompA genes and grouped in a cluster composed of R. montanensis (Rickettsiales: Rickettsiaceae), R. raoultii (Rickettsiales: Rickettsiaceae), R. aeschlimannii, R. massiliae (Rickettsiales: Rickettsiaceae), and R. rhipicephali (Rickettsiales: Rickettsiaceae) with htrA, while formed a separate clade with rrs. The pathogenicity of Rickettsia sp. sw should be further confirmed. These results expand the knowledge of the geographical distribution and vector distribution of spotted fever group rickettsiae in China and are useful for assessing the potential public health risk.

Rickettsia spp. and Ehrlichia spp. in Amblyomma ticks parasitizing wild amphibious sea kraits and yellow-margined box turtles in Okinawa, Japan

Recently, several tick-borne pathogens were detected in reptile-associated ticks. However, studies on the microorganisms in reptile-associated ticks in Japan are limited. This molecular survey thus aimed to identify and characterize tick-borne pathogens (Rickettsiaceae and Anaplasmataceae) in reptile-associated ticks in Japan. In total, 77 Amblyomma nitidum and 104 Amblyomma geoemydae were collected from wild amphibious sea kraits (Laticauda semifasciata, Laticauda colubrina, and Laticauda laticaudata) and from yellow-margined box turtles (Cuora flavomarginata evelynae), respectively. Conventional polymerase chain reaction was performed using the DNA extracted from the ticks to detect the selected pathogens. Sequencing analysis of four Rickettsia genes (gltA, ompA, ompB, and sca4) led to the identification of a putative novel Rickettsia sp. and Rickettsia aeschlimannii-like rickettsia in A. nitidum and A. geoemydae, respectively. Sequencing analysis of gltA and groEL of Anaplasmataceae revealed that the Ehrlichia spp. in these ticks were novel and related to Candidatus Ehrlichia occidentalis. This is the first study on the microorganisms in A. nitidium and the first record of Rickettsia and Ehrlichia in A. geoemydae. Further studies are required to understand their pathogenicity to humans and animals and their life cycle in the wild.

Amblyomma cordiferum Neumann, 1899 (Acari: Ixodidae) parasitizing reticulated pythons, Malayopython reticulatus (Schneider, 1801) (Reptilia: Pythonidae) in Peninsular Malaysia

Two ticks were collected from a reticulated python (Malayopython reticulatus) caught in Tumpat District, Kelantan, Peninsular Malaysia. The ticks were first identified as Ambylomma sp. through morphological comparison with identification keys. Determination of the tick species was made through PCR and sequencing. However, BLAST analysis revealed 85–88% sequence nucleotide identity with Amblyomma nitidum and Amblyomma geoemydae. Additionally, the morphological features of the ticks collected in this study did not match either A. nitidum or A. geoemydae. Further examination of the ticks confirmed the species as Amblyomma cordiferum. This is the first record of A. cordiferum DNA sequence with morphological support of colour illustrations for adult A. cordiferum. This is also the most recent record of this host association in Peninsular Malaysia. Information from this report can serve as a reference for species identification using the described morphology or molecular sequences.

The complete mitochondrial genome of Amblyomma geoemydae (Ixodida: Ixodidae)

The complete mitochondrial genome of Amblyomma geoemydae is reported for the first time in this study. Its entire mitogenome is 14,780 bp in length, contained 13 protein-coding genes, two ribosomal RNA genes, 22 transfer RNA genes, and two non-coding regions. The phylogenetic analysis by Maximum-likelihood method show that A. geoemydae and the others of genus Amblyomma are in the same clade, indicating that A. geoemydae belongs to the genus Amblyomma.

Long term persistence of introduced Amblyomma geoemydae tick population under indoor conditions in Austria.

An indoor terrarium population of Amblyomma geoemydae was established subsequent to the import of a single yellow-marginated box turtle Cuora flavomarginata. This indoor tick population revealed an unexpected resistance against de-ticking trials, with persistence between 2010 and 2015, when the ticks were successfully eliminated. Ticks were collected from the bodies and shells of turtles, as well as from terraria soil. Species diagnosis of ticks was carried out according to distinguishable morphological characters and supported by molecular analysis using DNA-barcoding. Introduced exotic ticks are potential vectors of pathogens and can have an impact on wildlife, domestic animals and the human population. This case emphasizes the need for sharp surveillance and control measures on imported reptiles.

Ticks in the Lion City: a preliminary review of the tick fauna of Singapore.

To this day, the tick fauna of Singapore remains poorly known. Although several studies of select species have been undertaken within Singapore, much of the information regarding Singaporean ticks is fragmentary. To facilitate future study of this group, the scattered information on Singaporean ticks is synthesised in the present work and includes a preliminary checklist of species reported to occur in Singapore, which comprised 14 species in 5 genera, with confirmed records of Argas pusillus, Amblyomma nitidum, Amblyomma varanense, Haemaphysalis doenitzi, Haemaphysalis nadchatrami, Haemaphysalis semermis, Ixodes granulatus, Rhipicephalus microplus, and Rhipicephalus sanguineus, and unconfirmed reports of Amblyomma cordiferum, Amblyomma geoemydae, Amblyomma helvolum, Amblyomma javanense and Amblyomma testudinarium.

Genera, subgenera, species and subspecies of hard ticks (Acari: Ixodidae) described, named, renamed or given new rank by Paul Schulze (1887–1949) and their current status

The names for 19 genera, 17 subgenera, 150 species and 150 subspecies of Ixodidae described by Paul Schulze, names changed by him after the revision of certain taxa, new ranks for names originally described by other authors, a few lapsus calamorum and some incorrect names ascribed to Schulze by other workers are discussed. Two genera (Cosmiomma and Nosomma) are valid, but most of Schulze’s subgenera are difficult to assess because of the current disarray that prevails at this classificatory level in the Ixodidae. Thirty-six of 150 species names discussed by Schulze are considered valid herein (3 species of Amblyomma, 1 Bothriocroton, 1 Cosmiomma, 6 Dermacentor, 4 Haemaphysalis, 5 Hyalomma, 13 Ixodes, 1 Nosomma and 2 Rhipicephalus), while 28 are valid species epithets that are currently assigned to different genera (e.g., Acarus undatus was formerly classified in the genus Aponomma but is currently known as Bothriocroton undatum). Twenty-nine of these valid species names were authored by Schulze or Schulze & Schlottke. The remaining species names are mostly synonyms or names that are incertae sedis, nomen nudum or lapsus calamorum. The 150 subspecific names are treated as synonyms of valid species or as nomina nuda, nomina dubia, incertae sedis or lapsus calamorum. Many names used by Schulze have been synonymized based on sound systematic research, but the justification for synonymizing other names is occasionally weak. It will be important to take these names into account if, as has already happened, future molecular and morphological studies result in the reinstatement of Schulze names currently considered synonyms. This situation has particular relevance for Amblyomma geoemydae, the Amblyomma marmoreum species complex, Amblyomma testudinarium, Oriental species of Dermacentor, Hyalomma in general, Ixodes arboricola, Ixodes canisuga, Ixodes lividus, Ixodes trianguliceps and Ixodes uriae. In conclusion, the prolific contributions of Paul Schulze have their weak points —the many species and subspecies of Hyalomma described by him are unjustified as is the alleged worldwide distribution of the former genus Aponomma—but his perception of the complexity of the genus Dermacentor (under Indocentor) in the Oriental and Australasian Zoogeographic Regions, as well as his understanding of tick biodiversity in these regions constitute seminal scientific achievements.

First record in Japan of tortoise tick Amblyomma Geoemydae (Cantor 1847) (Acari: Ixodidae) parasitizing Pryer’s keelback snake Hebius Pryeri (Boulenger, 1887) (Reptile: Colubridae)

ABSTRACTTwo larvae of tortoise tick Amblyomma geoemydae (Acari: Ixodidae) were collected from the fresh remains of a Pryer’s keelback snake Hebius pryeri (Boulenger, 1887), likely dead from accidental causes at the Kunigami-son in northern Okinawa Island, Okinawa Prefecture, Japan. This is the first such host record for A. geoemydae in Japan. Based on this finding, we have updated the available collection records of ticks found on Japanese snakes.

A novel relapsing fever Borrelia sp. infects the salivary glands of the molted hard tick, Amblyomma geoemydae

A novel relapsing fever Borrelia sp. was found in Amblyomma geoemydae in Japan. The novel Borrelia sp. was phylogenetically related to the hard (ixodid) tick-borne relapsing fever Borrelia spp. Borrelia miyamotoi and B. lonestari. The novel relapsing fever Borrelia sp. was detected in 39 A. geoemydae (39/274: 14.2%), of which 14 (14/274: 5.1%) were co-infected with the novel relapsing fever Borrelia sp. and Borrelia sp. tAG, one of the reptile-associated borreliae. Transstadial transmission of the novel relapsing fever Borrelia sp. occurred in the tick midgut and the salivary glands, although Borrelia sp. tAG was only detected in the tick midgut. The difference of the borrelial niche in molted ticks might be associated with borrelial characterization.

The ixodid ticks collected from dogs and other animals in Taiwan and Kinmen Island

A total of 594 ticks were collected from the environment, dogs, cattle, goats, small mammals, birds, a turtle, a snake, a bat and a human. Morphological identification of 509 ticks resulted in the listing of the following species: Rhipicephalus sanguineus, Rhipicephalus haemaphysaloides, Rhipicephalus microplus, Haemaphysalis warburtoni, Haemaphysalis phasiana, Haemaphysalis campanulata, Ixodes acutitarsus and Amblyomma geoemydae.

Characterization of reptile‐associated Borrelia sp. in the vector tick, Amblyomma geoemydae, and its association with Lyme disease and relapsing fever Borrelia spp.

The genus Borrelia is arthropod-borne infectious agents in vertebrates, and is classified into Lyme disease (LD) Borrelia spp. and Relapsing fever (RF) Borrelia spp. In addition to these Borrelia groups, we recently reported reptile-associated (REP) Borrelia spp. from reptiles and from hard-bodied ticks, which probably transmitted the REP Borrelia spp. In this study, we investigated the presence of REP Borrelia sp. in moulted ticks, and found that trans-stadial transmission of REP Borrelia sp. occurred in the midgut, while it was observed that REP Borrelia sp. entered the salivary gland during blood-feeding. This characteristic is also found in LD Borrelia spp., which are also transmitted by hard-bodied ticks. Although phylogenetic analysis demonstrated that REP Borrelia spp. are similar to RF Borrelia spp., the ecology of the spirochaetes within the vector ticks is different for REP Borrelia spp. and RF Borrelia spp. Elucidation of the evolutionary history of the genus Borrelia and its adaptation to ticks promises to be of great interest to researchers of vector-borne microorganisms.

Amblyomma geoemydae (Cantor) (Acari: Ixodida: Ixodidae): first report from the Arakan forest turtle, Heosemys depressa (Anderson) (Reptilia: Testudines: Emydidae), and first documented occurrence of this tick in the Union of Myanmar

ABSTRACT A single engorged nymph of Amblyomma geoemydae (Cantor) (Acari: Ixodida: Ixodidae) was collected from the Arakan forest turtle, Heosemys depressa (Anderson) (Reptilia: Testudines: Emydidae), in the Rakhine Yoma Elephant Range, south-western Myanmar. This is a new host record and constitutes the first documented Myanmar collection of A. geoemydae, a widespread tortoise and turtle tick in South and South-East Asia. Six collections of Amblyomma supinoi Neumann, an uncommon testudine parasite, are also reported.

B33 石垣島におけるカメキララマダニ幼虫の人体寄生例(一般講演,演題要旨,第58回日本衛生動物学会大会)

B33 石垣島におけるカメキララマダニ幼虫の人体寄生例(一般講演,演題要旨,第58回日本衛生動物学会大会)

Introduction of the exotic ticks Amblyomma humerale koch and Amblyomma geoemydae (Cantor) (Acari: Ixodidae) into the United States on imported reptiles

This is the first report of the reptilian ticks Amblyomma humerale Koch and A. geoemydae (Cantor) in the United States. Both were introduced into Florida on imported reptiles, A. humerale on a yellow-footed tortoise (Geochelone denticulata) from Guyana in South America and A. geoemydae on keeled box turtles (Pyxidea mouhotii) and black-breasted leaf turtles (Geoemyda spengleri) from Asia. Information on the host range, geographical distribution and life cycle of each tick is reviewed.