The Wigner–Ville distribution (WD) and ambiguity function (AF) are serviceable tools for the global analysis of non-stationary signals. However, when dealing with time-varying signals that exhibit changing characteristics and require real-time signal processing, they have high limitations due to the existence of cross-items. In this paper, we propose the short-time Wigner–Ville distribution (STWD) as a novel approach that effectively analyzes time-varying signals with changing characteristics. First, the definition of STWD and short-time ambiguity function (STAF) and their properties are advanced. Next, three uncertainty principles that define the lower bound for the product of signal spread and its bandwidth are derived. Then, the discrete STWD (DSTWD) and the process of computerization are also proposed which can be used more widely in practice. Finally, the optimization of window functions based on Rényi entropy is discussed. Simulation experiments show that the STWD can be effectively applied for the analysis of local change characteristics and real-time processing of signals with valid and accurate results.