Parathyroid allotransplantation is an emerging treatment for severe hypoparathyroidism. Ensuring the viability and functional integrity of donor parathyroid glands following procurement is essential for optimal transplantation outcomes. Cellular viability, calcium-responsive hormone secretion, and gland xenograft survival were assessed in a series of deceased donor parathyroid glands following a two-stage procurement procedure recently developed by our group (en bloc cadaveric dissection with subsequent gland isolation after transport to the laboratory). Parathyroid glands resected in this manner and stored up to 48h in 4°C University of Wisconsin (UW) media retained invitro viability with no induction of hypoxic stress (HIF-1α) or apoptotic (caspase-3) markers. Exvivo storage did not significantly affect parathyroid gland calcium sensing capacity, with comparable calcium EC50 values and suppression of parathyroid hormone secretion at high ambient calcium concentrations. The isolated glands engrafted readily, vascularizing rapidly invivo following transplantation into mice. Parathyroid tissue retains viability, calcium-sensing capacity, and invivo engraftment capability after en bloc cadaveric resection, exvivo dissection, and extended cold storage.