Amazon Basin Research Articles

Stable isotope ecology of terra preta in Caxiuanã National Forest, Brazil

The Amazon Basin currently hosts the world’s largest pool of terrestrial biodiversity and prior to European colonization of the Americas there were large human communities living in parkland ecosystems. We examine the formation of archaeological sites in the northeast sector of the Caxiuanã National Forest (CNF) using light stable isotopes of nitrogen and carbon, total carbon and nitrogen and Optically Stimulated Luminescence to characterize long-term human landscape management practices. Previous research in the CNF has documented differences in pH, calcium, total organic carbon (TOC) and nitrogen (TN) between terra preta and terra marrom contexts as well as different forest structures based on remote sensing analysis. Therefore, we adopt a comparative approach, examining the formation processes of on-site (terra preta), near-site (terra marrom) and offsite (latosol) contexts. TOC and TN values obtained in our study augment and support previous research demonstrating significantly higher on-site values relative to near-site and off-site. However, the stable isotopes (δ13C, δ15N) assayed from terra preta, terra marrom and latosols show statistically overlapping values, indicating the persistence of closed canopy in off-site and near-site contexts and the use of this canopy in the formation of on-site soils (terra preta). Our results corroborate the hypothesis that closed canopy ecosystems and human settlements persisted in the Amazon for thousands of years and formed the foundation of the region’s rich biodiversity.

A new species of Lelegeis Champion, 1886 from lowland natural forest of the Amazon basin of Peru (Coleoptera: Tenebrionidae, Diaperini).

A new species of Lelegeis Champion, 1886 from lowland natural forest of the Amazon basin of Peru (Coleoptera: Tenebrionidae, Diaperini).

Paleomagnetism of the Penatecaua magmatism: The CAMP intrusive rocks in the Amazonas Basin, northern Brazil

The Penatecaua magmatism (∼201 Ma) is part of the Central Atlantic Magmatic Province (CAMP) and is represented by voluminous sills in the Amazonas Basin, north Brazil. The sills appear south of the Amazonas River, particularly in the Medicilândia, Placas, and Rurópolis cities. To the north of the river, near Monte Alegre and Alenquer, smaller sills and NNE-SSW dikes prevail. Paleomagnetic data from 28 sampling sites of sills and dikes from all areas gave consistent results of normal polarity. Despite the large area of occurrence, the VGPs show small dispersion, consistent with a very brief emplacement time, as indicated by the radiometric ages. However, some sites, mainly from Alenquer and the southern sills, gave anomalous directions that may represent the record of a transitional geomagnetic field. The calculated paleomagnetic pole includes former data from Guerreiro and Schult (1986) plotting at 260.1°E 77.5°S (N = 30; A95 = 3.8°; k = 48) and agrees with other high-quality CAMP poles for South America.

Assessing charcoal and phytolith signals for pre-Columbian land-use based on modern indigenous activity areas in the Upper Xingu, Amazonia

The nature and extent of past indigenous transformations in the Amazon basin is an actively debated topic, and one that has important implications for both conservation policy and the cultural heritage of its indigenous and traditional populations. The use of charcoal and phytoliths to measure past human impacts in non-lacustrine settings has become especially influential in this debate but has also generated disagreement among scholars regarding the possible limits of these proxies for detecting ancient land-use. To contribute empirical data to this issue, our paper presents the first attempt to study charcoal and phytolith signals from areas of modern indigenous land-use, in the Xingu Indigenous Territory, southern Amazonia. Our findings show that, while charcoal and early successional herb phytoliths are good indicators of land-use intensity, certain types of land-use leave subtler traces in the phytolith record that can hinder their detection. We demonstrate how using finer sampling resolution and comparing local proxy data on their own terms are necessary steps in order to identify trends in human land-use across time and space.

First report of a histozoic Henneguya (Cnidaria, Endocnidozoa) infecting a synbranchid potamodromous fish from South America: Morphostructural and biological data

In this study, a Henneguya myxosporean species is described to infect an ecological, biological, and evolutionary important fish from Amazon biome. The myxosporean was found in the skin of only one specimen of marbled swamp eel, Synbranchus marmoratus caught in a small stream from Peruvian Amazon floodplain. Mature myxospores have ovoid shape from the valvular view, measuring 32.2 ± 0.6 μm (31.6–32.8) in total length, 21.5 ± 0.3 μm (21.2–21.8) in spore body length, 11.7 ± 0.5 μm (11.2–12.2) in width and 10.6 ± 0.9 μm (9.7–11.5) in thickness. Non-bifurcate caudal appendage, measuring 10.7 ± 0.4 μm (10.3–11.1) in length. Two polar capsules elongated aubergine in shape, equal in size and measuring 4.9 ± 0.2 μm (4.7–5.1) in length and 3.1 ± 0.5 μm (2.6–3.6) in width. Polar tubules coiled in 7–8 turns. This is the first report of a Henneguya species parasitizing a fish of the order Synbranchiformes from Amazon basin and the first to describe this parasite infecting a potamodromous fish from South America.

Amazonian ichthyoplankton assessment via DNA metabarcoding: A baseline for detecting spawning sites of migratory fishes

The Neotropical fish fauna comprises approximately 6080 species, of which almost 3000 inhabit the Amazon River basin. However, the construction of hydroelectric dams has threatened this biodiversity by habitat fragmentation. To uncover the putative effect of river impoundment on migratory fish species, over 5400 eggs and larvae were collected from the Teles Pires River, including five tributaries and the hydroelectric reservoir, which were identified using the COI metabarcoding approach. Identification of early life stages allowed the detection of >40 fish species, including at least 27 migratory species. Fish eggs were assigned to nine families, 13 genera, and 23 species, whereas larvae were attributed to 12 families, 18 genera, and 34 species. Eggs and larvae of migratory fishes were primarily present in tributaries upstream of the dam, with fewer in the reservoir. A putative impact of the dam on the ichthyoplankton community was detected, with a significant difference found between the reservoir and tributaries for larval species richness (r = 0.822, p = 0.0211). In contrast, no significant difference was recovered among tributaries for both eggs (r = 0.213, p = 0.2657) and larvae (r = 0.378, p = 0.0696). However, a change in ichthyoplankton assemblage was observed between tributaries, as revealed by beta diversity analyses. These results highlight the potential of ichthyoplankton metabarcoding for studying and biomonitoring of migratory fishes, as well as providing relevant data concerning the identification of spawning sites to set guidelines for fisheries management and effective conservation of fish populations.

Epidemiology and Genetic Diversity of Hepatitis B Virus and Hepatitis Delta Virus Infection in Indigenous Communities in Colombia.

Despite the universal vaccination program, there are still regions and territories with a high prevalence of Hepatitis B Virus infection (HBV), such as the Amazon basin, where several indigenous communities live. Additionally, Hepatitis Delta Virus (HDV) is a defective that requires the hepatitis B surface antigen (HBsAg) for the assembly and release of de novo viral particles. Therefore, hepatitis D could be the result of HBV/HDV coinfection or HDV superinfection in individuals with chronic hepatitis B. Among the high prevalence HDV populations are indigenous communities of America. This study aims to describe and characterize the frequency of HBV and HDV infection, viral genotypes and HBV immune escape mutants in indigenous populations from different regions of Colombia. The diagnosis of hepatitis B and hepatitis D was confirmed by serological markers. Moreover, the HBV and HDV genome were amplified by PCR and RT-PCR, respectively, and, subsequently, the phylogenetic analysis was performed. We characterized 47 cases of chronic hepatitis B, 1 case of reactivation and 2 cases of occult hepatitis B infection (OBI). Furthermore, a high prevalence of HDV infection was identified in the study population (29.33%, 22/75) and the circulation of several HBV genotypes and subgenotypes (F1b, F3, F4, and D). Interestingly, this is the first report of the HDV genotype I circulation in this country. These findings demonstrated that HBV and HDV infections are still public health problems in indigenous communities in Colombia.

Association of mycoheterotrophic Gentianaceae with specific Glomus lineages.

Some plant species took an alternative evolutionary pathway in which they lost their photosynthetic capacity to depend exclusively on carbon supplied by arbuscular mycorrhizal fungi (AMF) in an association called mycoheterotrophy. Among them is Voyriella parviflora, a species of the family Gentianaceae, which is found in tropical regions such as the Amazon basin. Here, we assessed the identity of AMF symbionts associated with this species. DNA was isolated from eight Gentianaceae specimens and from litter and surrounding roots of photosynthetic plants. The atp1 gene was amplified by Sanger sequencing to determine the taxonomic affiliation of the mycoheterotrophic plants. A 280bp region of the 18S rRNA gene of AMF was amplified with primers NS31/AML2 by high-throughput sequencing. The mycoheterotrophic specimens were assigned to V. parviflora with a bootstrap support of 72%. Glomus was the most abundant AMF genus, both in the mycoheterotrophic plants and in the litter and roots of photosynthetic plants. In addition, a few Glomus genotypes were abundantly enriched in the mycoheterotrophic plants, with only a few specimens colonized by Gigaspora, Acaulospora, and Scutellospora in a low proportion. These genotypes formed a cluster within a larger clade, suggesting that V. parviflora shows a preferential association with a narrow Glomus lineage which is not phylogenetically close to a previously identified V. parviflora's associated lineage. Furthermore, detecting fungi from other families suggests that V. parviflora is colonized by other genera, although with low frequency. These findings provide new insights into the association between AMF and mycoheterotrophic species and highlight the importance of considering trap culture-independent approaches in understanding this symbiosis.

Spatial+: A new cross-validation method to evaluate geospatial machine learning models

Random cross-validation (CV) is often used to evaluate geospatial machine learning models, particularly when a limited amount of sample data are available, and collecting an extra test set is unfeasible. However, the prediction locations can be substantially different from the available sample, leading to over-optimistic evaluation results. This has fostered the development of spatial CV methods. Yet these methods only focus on spatial autocorrelation and cannot sufficiently guarantee that the validation subset is a good proxy of the test set with significant differences. In this paper, we propose the spatial+ cross-validation (SP-CV) method. This method, which considers both the geographic and feature spaces, is composed of two stages. The first stage addresses spatial autocorrelation issues by using agglomerative hierarchical clustering to divide the available sample into blocks. The second stage deals with multiple sources of differences. It uses cluster ensembles to split the blocks into training and validation folds based on the locations of the sample data and the values of the covariates and target variable. The proposed method is compared against random and block CV methods in a series of experiments with Amazon basin above ground biomass and California houseprice datasets. Our results show that SP-CV provided the smallest error differences with respect to the reference error. This means that SP-CV produced more representative splits and led to more reliable model evaluations. It suggests that a reliable model evaluation requires to consider both the geographic and the feature spaces in a comprehensive manner.

Fish habitat use in the Upper Beni mountain streams (Amazon Basin, Bolivia)

Fish habitat use in the Upper Beni mountain streams (Amazon Basin, Bolivia)

Impacts of increasing greenhouse gas concentrations and deforestation on extreme rainfall events in the Amazon basin: A multi‐model ensemble‐based study

AbstractThe main objective of this study was to evaluate the effects of increasing greenhouse gases (GHGs) and total deforestation of the Amazon on extreme rainfall events in the Amazon basin. In order to quantify these impacts, numerical experiments were performed with the Eta regional climate model forced from initial and boundary conditions from the BESM, HadGEM2‐ES, and MIROC5 earth system models. In the experiment related to the increase in GHGs, numerical simulations were performed according to the IPCC RCP8.5 scenario. The effect of deforestation was quantified via an experiment in which the forest in the Amazon basin was replaced by areas of degraded pasture in the Eta model. For the analyses of the changes in extreme rainfall events, the multi‐model ensemble technique was used. The results were evaluated in terms of anomalies relative to the sensitivity and control experiments. In the results, it was observed that in an RCP8.5‐type GHG emission scenario there is a statistically significant increase in the maximum number of consecutive days without rain, a reduction in the maximum number of consecutive rainy days, reduction in total annual precipitation, and reduction in maximum annual precipitation accumulated over one and 5 days, respectively. The results for the scenario with increased GHGs and large‐scale deforestation in the Amazon basin are similar to the RCP8.5 scenario, but the intensity of changes in climate indices is significantly greater. It was also verified that the changes in the climatic indices are strongly associated with alterations in the energy balances at the surface and, consequently, in the large‐scale circulation. In general, it can be highlighted that the climate in the Amazon region is strongly dependent on the presence of the forest.

Amazonian earthworm biodiversity is heavily impacted by ancient and recent human disturbance

Despite the importance of earthworms for soil formation, more is needed to know about how Pre-Columbian modifications to soils and the landscape. Gaining a deeper understanding is essential for comprehending the historical drivers of earthworm communities and the development of effective conservation strategies in the Amazon rainforest. Human disturbance can significantly impact earthworm diversity, especially in rainforest soils, and in the particular case of the Amazonian rainforest, both recent and ancient anthropic practices may be important. Amazonian Dark Earths (ADEs) are fertile soils found throughout the Amazon Basin, created by sedentary habits and intensification patterns of pre-Colombian societies primarily developed in the second part of the Holocene period. We have sampled earthworm communities in three Brazilian Amazonian (ADEs) and adjacent reference soils (REF) under old and young forests and monocultures.To better assess taxonomic richness, we used morphology and the barcode region of the COI gene to identify juveniles and cocoons and delimit Molecular Operational Taxonomic Units (MOTUs). Here we suggest using Integrated Operational Taxonomical units (IOTUs) which combine both morphological and molecular data and provide a more comprehensive assessment of diversity, while MOTUs only rely on molecular data. A total of 970 individuals were collected, resulting in 51 taxonomic units (IOTUs, MOTUs, and morphospecies combined). From this total, 24 taxonomic units were unique to REF soils, 17 to ADEs, and ten were shared between both soils. The highest richness was found in old forest sites for ADEs (12 taxonomic units) and REFs (21 taxonomic units). The beta-diversity calculations reveal a high species turnover between ADEs and REF soils, providing evidence that ADEs and REFs possess distinct soil biota. Furthermore, results suggest that ADE sites, formed by Pre-Columbian human activities, conserve a high number of native species in the landscape and maintain a high abundance, despite their long-term nature.

The Tree of Abundance: On the Indigenous Emergence in Contemporary Latin American Art

The Tree of Abundance is an origin story for many nations in the Amazon basin. It recounts a time when all people(s) lived under a mother tree, until those with an ax arrived and the tree collapsed. This is the act of coloniality, which produced a new landscape. The story serves as a conceptual metaphor to analyze the production of an emerging generation of contemporary visual makers of indigenous origin. These cultural producers are set in a historical context, which represents long temporalities of cultural-production resistance and re-existence in Latin America (called here Abya Yala). The text introduces a way to rethink contemporary art in the region under conditions of coloniality and names the artists “embodied territories” since they have particular connections to the places they live and work. This article is organized into three parts presenting artwork by several indigenous and intercultural subjects (with emphasis on those living in indigenous territories of Colombia): (1) A short genealogy from modernity to contemporaneity brings indigenous cultural production to the academic space as another source for a critical understanding of the lived experience in Abya Yala. (2) An account of themes derived from the contested histories highlights how indigenous and intercultural artists produce responses to them. (3) The genealogy and themes are then set in spatial terms offering two case studies, on one hand, the toppling of historical figures by indigenous activists as performance in the public space and, on the other, the exhibitions “Visual Sovereignty” and the “Indigenous Salon Manuel Quintín Lame”. The article concludes stressing how this emerging generation builds on long genealogies of sovereign representation, responding with a wide range of contemporary means (visual, textual, bodily, and multimedia) to issues that still affect their communities (land grabs, resource extraction, racialization, marginality, etc.). Adaptation, resistance, and re-existence occur when embodied territories recognize historical realities (time), location (space), and forms of liberation (action) within coloniality.

Occurrence, sources, and ecological risks of polycyclic aromatic hydrocarbons (PAHs) in the Amazon river

The Amazon is the largest river by discharge volume and one of the most biodiverse biomes in the world. Lately, there has been a rapid increase of the urban population in the region, which has been translated into a growing emission of organic contaminants such as polycyclic aromatic hydrocarbons (PAHs) into surface water bodies. This study provides the most comprehensive evaluation of the PAH contamination levels in surface waters of the Amazon basin. We investigated the occurrence and potential sources of 16 priority PAHs and characterised their risks for freshwater ecosystems. For this, we took 40 water samples from different sites along the Brazilian part of the Amazon River, including three major tributaries, and smaller rivers crossing the main urban areas. The results of this study show that PAHs are widespread contaminants in rivers of the Brazilian Amazon. The sum of the total concentration of the 16 priority PAHs reached values of 134 ng L−1 in the Amazon River, and 163 ng L−1 near densely populated areas. On the other hand, the total PAH concentration was generally lower in the monitored tributaries. In most samples, the contamination pattern was dominated by high molecular weight PAHs, suggesting a major contribution of pyrogenic sources, although petrogenic contamination was also present in some locations near urban areas. We assessed ecological risks posed by PAH mixtures using a hazard index. The results indicated that PAH contamination is not likely to pose direct toxic effects for Amazonian freshwater organisms, however continued monitoring is recommended near densely populated areas.

Spatial and temporal population genetic analysis of Semaprochilodus insignis (Prochilodontidae), an overexploited fish from the Amazon basin.

Semaprochilodus insignis is a migratory fish of commercial and subsistence importance to communities in the Amazon. Despite the high intensity of exploitation, recent studies have not been carried out to assess the genetic status of its stocks. This study is the first to estimate genetic diversity and to test the existence of spatial and temporal structuring of S. insignis through sequencing of the mtDNA control region (n=241) and eight microsatellite loci (n=180) of individuals sampled at 11 sites distributed in the Brazilian Amazon basin. Results for both markers were congruent, revealing a homogeneous genetic diversity in all the sampled locations, in addition to the absence of spatial and temporal genetic structure, indicating that the species forms a large panmictic population in the Brazilian Amazon. Although overfishing does not yet appear to have affected the levels of genetic variability of S. insignis, signals of reduction of the effective population size and a bottleneck provide an early alert to the effects of overfishing. Thus, the ever-decreasing populations may threaten S. insignis in the future. Therefore, it is hoped that the results of this study may contribute to the elaboration of management plans or any other measures that aim at the management and conservation of this species of great importance for the Amazon basin.

A copula-supported Bayesian framework for spatial downscaling of GRACE-derived terrestrial water storage flux

The GRACE and GRACE-FO satellite missions provide mass variations as a fundamentally new observation type for a broad spectrum of novel applications in Earth science disciplines, including oceanography, geophysics, hydrology, and hydrometeorology. Despite all the key findings in hydrology, the utility of GRACE-derived Terrestrial Water Storage Anomaly (TWSA) and its time derivative Terrestrial Water Storage Flux (TWSF) have mainly been limited to large catchments due to their coarse spatial resolution. Here, we propose a method to downscale TWSF by incorporating available finer-resolution data. We determine the downscaled TWSF and its uncertainty within a proposed Bayesian framework by incorporating the fine-scale data of TWSF and Soil Moisture Change (SMC) from different available sources. For the Bayesian ingredients, we rely on GRACE data to obtain the prior and rely on copula models to obtain nonparametric likelihood functions based on the statistical relationship between GRACE TWSF with fine-scale TWSF data and SMC. We apply our method to the Amazon Basin and assess the performances of our products from various fine-scale input datasets of TWSFs and SMCs. Given the lack of ground truth for TWSF, we validate our results against space-based Surface Water Storage Change (SWSC) in the Amazon river system and also against the Vertical Crustal Displacements Rate (VCDR) observed by the Global Positioning System (GPS). Overall, the results show that the proposed method is able to estimate a downscaled TWSF, which is informed by GRACE and fine-scale data. Validation shows that our downscaled products are better anticorrelated with VCDR (−0.81) than fine-scale TWSF (−0.73) and show a mean relative RMSE of 26% with SWSC versus 70% for fine-scale TWSF. The proposed methodology can be extended to other coarse scale datasets, which are crucial for hydrological application at regional and local scales.

Serine metalloprotease with plasmin-like fibrinolytic activity of Serratia marcescens isolated from the Amazon basin

Research on new fibrinolytic agents has been oriented towards the discovery of microbial proteases with plasmin-like activity, as alternative thrombolytic agents for thrombosis treatment. This study reports the characterization of an extracellular serine metalloprotease of approximately 56 kDa, with fibrinolytic and fibrinogenolytic activity, isolated from a S. marcescens strain obtained from the Amazon (CBAM 519). The enzyme showed optimum activity at pH 9 and temperature of 37 °C. Activity was reduced in the presence of Na+, Cu2+ and Fe2+ ions, and increased with Mn2⁺. The enzyme did not show hemolytic activity, nor did it activate plasminogen, but it effectively hydrolyzed fibrin and fibrinogen, rapidly degrading all fibrinogen chains Aα, Bβ and γ. Therefore, fibrinolysis was promoted only by the direct route, with a fast acting, potent fibrinolytic activity. Based on this, we expect the enzyme to be a protease of the plasmin type that directly degrades fibrin. These characteristics demonstrate great potential for its application in the treatment of thrombosis.

Assessing wildfire activity and forest loss in protected areas of the Amazon basin

Wildfires annually burn extensive areas in the Amazonia. Still, more is needed to know about the complex combination of triggering socioeconomic factors and environmental policies that motivate and explain the growing wildfire activity and forest losses. This study assessed wildfire occurrence, exposure, and transmission to natural forests in protected natural areas (n = 438) from 9 countries encompassing 140 million hectares of the Amazon basin. The fire ignitions and burned areas were first detected using remote sensing products from 2001 to 2018. We then conducted a wildfire transmission analysis to determine the neighboring fire source hotspots and land uses associated with human-caused ignitions encroaching on protected areas. Finally, we analyzed the environmental policies to understand the failure of such regulations to prevent the escape of fires burning large areas within protected sites. The results showed that recurrent wildfire hotspots cover a reduced portion but account for the bulk of the burned area. We found that, on average, wildfires burned 86,700 ha yr−1 of natural forests in protected sites, of which 10.5% corresponded to large incoming fires. Savanna and grasslands concentrated 29% and 41% of fire ignitions, where farmers and ranchers use fire periodically to clear extensive grazing properties. However, this high ignition density did not necessarily correlate with burned areas. The lack of a clear understanding of environmental policies and insufficient law enforcement was likely the main reasons for the continuous wildfire transmission to protected forests. The quantitative outcomes provide science-based criteria for detecting the high-priority areas within the Amazon where management efforts could help reverse the growing wildfire risk to protected natural forests.

Distinct Non-conservative Behavior of Dissolved Organic Matter after Mixing Solimões/Negro and Amazon/Tapajós River Waters.

Positive and negative electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry and 1H NMR revealed major compositional and structural changes of dissolved organic matter (DOM) after mixing two sets of river waters in Amazon confluences: the Solimões and Negro Rivers (S + N) and the Amazon and Tapajós Rivers (A + T). We also studied the effects of water mixing ratios and incubation time on the composition and structure of DOM molecules. NMR spectra demonstrated large-scale structural transformations in the case of S + N mixing, with gain of pure and functionalized aliphatic units and loss of all other structures after 1d incubation. A + T mixing resulted in comparatively minor structural alterations, with a major gain of small aliphatic biomolecular binding motifs. Remarkably, structural alterations from mixing to 1d incubation were in essence reversed from 1d to 5d incubation for both S + N and A + T mixing experiments. Heterotrophic bacterial production (HBP) in endmembers S, N, and S + N mixtures remained near 0.03 μgC L-1 h-1, whereas HBP in A, T, and A + T were about five times higher. High rates of dark carbon fixation took place at S + N mixing in particular. In-depth biogeochemical characterization revealed major distinctions between DOM biogeochemical changes and temporal evolution at these key confluence sites within the Amazon basin.

Nova ocorrência de Crinoidea no Pensilvaniano da Formação Itaituba, Pedreira Caltarém, norte da Bacia do Amazonas

Crinoidea from the Pennsylvanian deposits of the Itaituba Formation, Northern Amazonas Basin. The Itaituba Formation comprises part of the marine section of the Tapajós Group in the Amazonas Basin. This unit mainly comprises Pennsylvanian carbonate deposits associated with various marine invertebrate fossils. In this work, we present new records of crinoids in the Itaituba Formation in the Caltarém quarry, north platform of the Amazonas Basin. There are described and taxonomically identified, two genera of crinoids, through the study of columnals and pluricolumnals, in addition to the interpretation of the depositional environment through the analysis of carbonate microfacies. The identified specimens correspond to the species Cyclocaudex aff. typicus Moore & Jeffords, and to the genus Pentaridica Moore & Jeffords (first record in the Itaituba Formation). The textural, compositional, and paleontological analysis suggests four standard microfacies MPF 09, 10, 16, 23) and two faciological zones (ZF 7 E 8). The bioturbated bioclastic wackestones (MFP 09) and bioclastic wackestones/packstones (MFP 10) microfacies, with fossils of brachiopods, crinoids, echinoids, miliolid and fusulinid foraminifera, trilobites, and bryozoans, characterize an interior shelf environment, with a connection to an open marine circulation (ZF 7). The peloidal wackestones (MFP 16) and mudstones (MFP 23) microfacies, with a fossiliferous assemblage limited to brachiopods, echinoids and miliolid foraminifera, characterize a restricted lagoon environment (ZF 8). Paleontological data and microfacies analysis indicate the development of a lagoon environment with a diverse paleofauna of marine invertebrates in the carbonate platform of the northern bord of the Amazonas Basin, in the Monte Alegre County, during the Pennsylvanian. Keywords: taxonomy, echinoderms, microfacies, paleoenvironment, Pennsylvanian.