Background: Intestinal parasitic infections are among the most common chronic human infections in developing countries; particularly within the tropical and subtropical regions. An ethno-botanical survey was carried out to document medicinal plants used for the treatment of intestinal worms in Amathole District Municipality of the Eastern Cape Province (ECP), South AfricaMaterials and methods: A questionnaire-guided interview of the indigenous people by random sampling was done with the help of an interpreter during the survey of the district.Results: Medicinal plants mostly used for traditional management of intestinal worms were selected from 13 plant families: Alliaceae, Anacardiaceae, Asphodelaceae, Asteraceae, Crassulaceae, Dracaenaceae, Fabaceae, Hypoxidaceae, Lamiaceae, Longaniaceae, Punicaceae, Polygonaceae, and Verbenaceae. Out of these, Hypoxis hererocallidea (17%), Strychonos henningsii (14%), Rumex lanceolatus (12%), Ozoroa mucronata (9%) and Acacia karoo (9%) belonging to the families Hypoxidaceae, Longaniaceae, Polygonaceae, Anacardiaceae and Fabaceae respectively were found as the most cited, the least cited plant was Cotyledon orbiculata (2%). Leaves and roots from these plants were found to be commonly used (36%), followed by the root (27%), bark (14%), the bulb and stem (9%), and rhizome collectively constitute (5%). Oral method of administration by decoction (50%) and infusion (39%) was however found to be a common method by respondents.Conclusion: The current study however showed that ethno-pharmacological knowledge of the traditional healers in ECP largely depends on naturally growing species, documentation of which will go a long way in validating the therapeutic uses and safety of these plants as anthelmintic plants.Keywords: Ethno-botanical survey, Eastern Cape Province, Amathole District, anthelmintic, indigenous recipes