Amaranthus Lividus Research Articles

The herbicidal activity and mechanism of Talaromyces purpureogenus CY-1 metabolites.

Studies have shown that Talaromyces can produce a large number of secondary metabolites in its metabolic process, many of which have good insecticidal, antibacterial, antitumor, antiviral and other biological activities. In order to explore the herbicidal activity and mechanism of Talaromyces purpureogenus CY-1, we determined the inhibitory effect of the fermentation broth of the CY-1 strain on weeds, identified the major active components, and further investigated the herbicidal mechanism. The results showed that CY-1, with IC50 values of 5.40 g/L and 4.39 g/L, respectively, exhibited good herbicidal activity against Xanthium sibiricum and Amaranthus lividus. Spraying CY-1 strain fermentation broth on redroot pigweed resulted in plant protection efficiencies and fresh weight protection efficiencies of 83.7% and 87%, respectively. The active component identified in the broth was 2-(3-hydroxybenzoyloxy) acrylic acid. Treatment of Amaranthus lividus with 2-(3-hydroxybenzoyloxy) acrylic acid resulted in trends of increasing superoxide dismutase activity, peroxidase activity, respiratory rate, cytochrome oxidase activity, and soluble protein content, followed by a decrease. Peroxidase activity, relative conductance and malondialdehyde content gradually increased, while acetyl lactate synthase and glutamine synthetase initially decreased and gradually returned to normal. The soluble sugar content showed a gradual decrease. The findings of the present study indicate that 2-(3-hydroxybenzoyloxy) acrylic acid can induce oxidative stress in plants, damaging plant cell walls, weakening respiratory activity, inhibiting protein synthesis and sugar metabolism, ultimately leading to weed wilting and death. © 2024 Society of Chemical Industry.

Evaluating the genetic parameters, heritability, and genetic diversity of datashak (Amaranthus lividus) under hot summer growing conditions

Evaluating the genetic parameters, heritability, and genetic diversity of datashak (Amaranthus lividus) under hot summer growing conditions

Evaluating anthelmintic, anti-platelet, and anti-coagulant activities, and identifying the bioactive phytochemicals of Amaranthus blitum L.

BackgroundHighlighting affordable alternative crops that are rich in bioactive phytoconstituents is essential for advancing nutrition and ensuring food security. Amaranthus blitum L. (AB) stands out as one such crop with a traditional history of being used to treat intestinal disorders, roundworm infections, and hemorrhage. This study aimed to evaluate the anthelmintic and hematologic activities across various extracts of AB and investigate the phytoconstituents responsible for these activities.MethodsIn vitro anthelmintic activity against Trichinella spiralis was evaluated in terms of larval viability reduction. The anti-platelet activities were assessed based on the inhibitory effect against induced platelet aggregation. Further, effects on the extrinsic pathway, the intrinsic pathway, and the ultimate common stage of blood coagulation, were monitored through measuring blood coagulation parameters: prothrombin time (PT), activated partial thromboplastin time (aPTT), and thrombin time (TT), respectively. The structures of isolated compounds were elucidated by spectroscopic analysis.ResultsInterestingly, a previously undescribed compound (19), N-(cis-p-coumaroyl)-ʟ-tryptophan, was isolated and identified along with 21 known compounds. Significant in vitro larvicidal activities were demonstrated by the investigated AB extracts at 1 mg/mL. Among tested compounds, compound 18 (rutin) displayed the highest larvicidal activity. Moreover, compounds 19 and 20 (N-(trans-p-coumaroyl)-ʟ-tryptophan) induced complete larval death within 48 h. The crude extract exhibited the minimal platelet aggregation of 43.42 ± 11.69%, compared with 76.22 ± 14.34% in the control plasma. Additionally, the crude extract and two compounds 19 and 20 significantly inhibited the extrinsic coagulation pathway.ConclusionsThese findings extend awareness about the nutritional value of AB as a food, with thrombosis-preventing capabilities and introducing a promising source for new anthelmintic and anticoagulant agents.

<i>Amaranthus emarginatus</i> s.lat. (Amaranthaceae) in Italy

Amaranthus emarginatus belongs to the Amaranthus blitum complex, a critical taxonomic group. Within the variability of A. emarginatus, two taxa are often accepted, ‘emarginatus’ and ‘pseudogracilis’, which differ by habitus, synflorescence structure, and leaf size. However, their taxonomic value is debated, and A. emarginatus itself is sometimes included in A. blitum as subspecies. In this paper, molecular, morphological, phytogeographical, and ecological data are discussed to assess the most correct rank of these taxa. We conclude that A. emarginatus deserves specific distinctness from A. blitum, and A. pseudogracilis can be regarded as a subspecies of the former. This choice reflects both the molecular data and geographical distributions of A. blitum and A. emarginatus, i.e., Mediterranean area, Europe, and North Africa for the former vs. neotropics for the latter). Within A. emarginatus, in addition, to the different morphology, A. emarginatus var. pseudogracilis differs also on account of its ecological preferences, because it occurs in warm and humid areas, whereas A. emarginatus var. emarginatus is better adapted to cold and dry habitats. However, since molecular analyses cannot clearly separate the two taxa, we consider the subspecific rank as the most appropriate. We also found a further morphological difference in seed coat ornamentation, hitherto not pointed out: the shape of the peripheral cells is tetragonal in A. emarginatus subsp. pseudogracilis, but polygonal (cells with ≥ 5 sides) in subsp. emarginatus. Distributional maps and status assessment at regional level for the two taxa are provided. According to this revision, A. emarginatus subsp. emarginatus is to be regared as naturalized in central and southern Italy (Latium and Campania, respectively).

Determination of Total Antioxidant Activity & Target Micronutrient Contents of the Selected Nutri-Medicinal Plants

Nutri-medicinal plants may have a big role to play in the intervention to improve the nutritional status and manage immunosuppressive disorders in HIV/AIDS patients as well as the opportunistic ailments that arise from the same. These plants are readily available and affordable in our ecosystems and also contain micronutrients which in themselves may have antioxidant properties that are needed in the management of ailments in HIV/AIDS patients. The use of such plants rich in phytochemicals, as supplements and herbal remedies may slow down oxidative stress and the progression of HIV/AIDS. In this study, fifty randomly selected plants collected from four parishes of Namayumba sub-county, Wakiso district, Central Uganda were evaluated for total antioxidant capacity (TAC) using two assays 1,1-Diphenyl-2-picrylhydrazyl radical (DPPH) and Ferric Reducing Ability of Plasma (FRAP), and for micronutrients namely Zinc, Iron and Selenium. The results from TAC-DPPH ranged from 30.63±0.21% to 1.59±0.06% for Annona muricata and Spathodea campanulata respectively, and TAC-FRAP recorded a range of 5.16±1.39 mM/l to 0.08±mM/l for Amaranthus lividus and Spathodea campanulata respectively. Amaranthus lividus registered the highest Zinc content as 6567.65±20.81 mg/kg with Erythrina abyssinica exhibiting the lowest at 0.39±0.03 mg/kg. The leaves of a commonly consumed tuber Manihot esculenta had the highest Iron content at 1289.54±0.14 mg/kg and Kigelia africana with the lowest at 3.23±0.37 mg/kg. The study indicated that selenium is present in small amounts compared to Zinc and Iron; however, Amaranthus lividus and Spathodea campanulata registered the highest of 8.18±2.46 mg/kg and lowest of 0.20±0.004 mg/kg respectively. The study showed a wide range of antioxidant and micronutrient content in the plants studied indicating that a wide choice of nutri-medicinal plants is available with a large potential to provide nutritional and medicinal interventions to manage nutritional disorders and manage opportunistic ailments in HIV/AIDS patients

Aryl sulfoxide scaffold useful as herbicide

The escalating demand for effective and sustainable weed management strategies, driven by urbanization expansion, is a critical challenge. Herbicides are pivotal tools in modern agriculture, addressing this challenge. Developing novel herbicides with enhanced efficacy and minimal environmental impact is crucial for food security and ecological balance. While numerous herbicides have been developed with varying availability over time and regions, there's a continuous need for innovation. In this study, we explored relatively understudied sulfoxide-containing herbicides and synthesized a smaller yet substantial sulfoxide scaffold for herbicide development. Through screening Digitaria ciliaris (Retz.) Koeler, Amaranthus lividus L., and Solanum nigrum L., we observed promising herbicidal efficacy, especially against Wild Amaranth. Encouraged by preliminary findings, we recognize the potential for refining the core structure. In summary, we fashioned a structurally simple sulfoxide scaffold showcasing discernible herbicidal impact on broadleaf weeds.

Effects of seed treatments and storage duration on Myzus persicae (Hemiptera: Aphididae) and amaranth fresh leaf yield.

The green peach aphid, Myzus persicae (Sulzer) (Hemiptera: Aphididae), is a key insect pest of amaranth in East Africa. Pest management has been restricted to indiscriminate application of insecticides to foliage. Applying systemic insecticides to seeds has been shown to manage aphid infestations in other crop systems. We evaluated two commercially available seed treatments in East Africa, Apron Star (thiamethoxam 20 g/kg + metalaxyl-M 20 g/kg + difenoconazole 2 g/kg) and Menceron (imidacloprid 233 g/L + pencycuron 50 g/L + thiram 107 g/L) for their efficacy against M. persicae and impact on fresh leaf yield with two Amaranthus species, Amaranthus blitum (2 selections), Amaranthus hybridus (4 selections) and untreated control. Two storage periods (24 h and 3 months) with seed treatments were used. Each amaranth selection was treated individually with Apron and Monceren or untreated, and seeds were planted either 24 h or 3 months after treatment. Significant reduction in live aphids was observed with A. blitum and A. hybridus selections grown with seed treatment, at 6, 8 and 10 d after infestation (DAI) when compared with seeds grown without seed treatment. Untreated seeds of A. hybridus (selection 5) had significantly higher number of live aphids up to 243, greater percentage of damaged leaves and leaf damage score up to 84% and 64% respectively when compared with treated seeds of specific amaranth at 10 DAI. No significant difference was noted between seed treatment and storage time. Amaranth seeds treated with Monceren offered more protection against infestations of Myzus persicae than amaranth seeds treated with Apron under high tunnel experiments.

Taxonomic, nomenclatural and floristic review of Amaranthaceae of Greece and neighbouring countries

The history and progress of floristic knowledge on Amaranthaceae occurring in Greece and adjacent Balkan countries are briefly outlined and changes in family circumscription and generic delimitation are addressed. The new combinations Amaranthus blitoides [var. blitoides] f. thellungii (Sennen ex Thell.) Raus and A. blitoides [var. reverchonii] f. densifolius (Uline & W. L. Bray) Raus are published. The names Achyranthes argentea Lam. and Amaranthus commutatus A. Kern. ex Hayek are lectotypified, the former a synonym of Achyranthes sicula (L.) All , the latter of Amaranthus blitum L. Amaranthus commutatus A. Kern. has long been known as a designation with no nomenclatural value for a fictitious taxon but is still erroneously accepted in current floristic online sources. Citation: Raus Th. 2022: Taxonomic, nomenclatural and floristic review of Amaranthaceae of Greece and neighbouring countries. – Willdenowia 52: 335–357. Version of record first published online on 6 December 2022 ahead of inclusion in December 2022 issue.

In vivo and in silico evaluation of analgesic and hypoglycemic activities of Amaranthus blitum L.

Amaranthus blitum L. is a worldwide weed that originated in the Mediterranean region and now grows from the tropics to temperate zones, from Asian to southern African countries. It is commonly consumed as a cooked vegetable item. Amaranthus blitum L., on the other hand, has never been extensively investigated for its medicinal properties like analgesic and hypoglycemic potentials. Hence, using an in vivo and in silico method, assess the analgesic and hypoglycemic efficacy of Amaranthus blitum L. methanolic extract and its key phytoconstituents is the primary aim of this study. The acetic acid-induced writhing test and the hot plate test protocol were employed to assess the in vivo analgesic effectiveness of Amaranthus blitum L. methanolic extract (ABME) in Swiss albino mice of either sex. In a Stretozotocin (STZ) induced anti-diabetic test, the hypoglycemic activity was investigated. Admet-SAR web server and PyMOL (Version 1.7.4) software package were used to perform in silico activity of the isolated compounds and ADME analysis. The research employed methanolic extracts (ME) at dosages of 250 mg/kg and 500 mg/kg to assess analgesic and hypoglycemic effects. ME (250 mg/kg) and ME (500 mg/kg) showed substantial outcomes in the acetic acid-induced writhing test, with 39.48 ± 5.66% and 51.20 ± 3.78%, respectively. ME at a dosage of 500 mg/kg caused the highest latency time increase of 16.92 ± 0.94s in the hot plate test. In the context of in silico assessment, eleven chemicals fit Lipinski's five-condition criteria. The best docking score was for glycidyl oleate which is 6.3 kcal/mol against cyclooxygenase-2 enzyme (PDB ID: 5KIR) among these safe chemicals, which was practically identical to Celecoxib's (8.5 kcal/mol). In streptozotocin induced hypoglycemic test, significant decrease in plasma glucose level was observed for 500 mg/kg of methanolic extract. In silico study showed best docking score of 2,2-di‑tert-butylphenol with − 6.4 kcal/mol against sulfonylurea protein (PDB ID: 6JB3), compared to glibenclamide (− 9.4 kcal/mol). Our research demonstrates that phytoconstituents of Amaranthus blitum L. are very potential analgesic and hypoglycemic candidates. These compounds might be employed to develop an effective analgesic and anti-diabetic medication from a natural resource using advanced technologies.

Quantitative ethnoveterinary study on plant resource utilization by indigenous communities in high-altitude regions.

For millennia, ethnic knowledge has been intricately tied to local biodiversity and woven into the fabric of rural communities. Growing scientific evidence suggests that merging ethnic knowledge with new scientific findings can lead to socially acceptable and environmentally friendly approaches essential for the long-term prosperity of local communities. In the high-altitude region, where livestock raising is a key income source, and plant-based utilization for ethno-veterinary practices is widely practiced. In this context, this study was conducted with the aim of documenting the ethno-veterinary use of plant resources in different bio-geographical regions of Jammu and Kashmir's Himalayas (J & KH). Semi-structured interviews and group discussions were used to collect information. Principal component analysis (PCA) and Pearson correlation were conducted to analyze the data. We documented 148 species from 53 families that locals used for various purposes: medicine, fodder, tonic, antidote, magic, and also used to protect themselves from ectoparasite such as Pediculus humanus capitis by the local inhabitants. There were significant differences in the relative usage of plant resources across the three biogeographic regions. Comparatively, the highest number (41%) of plant species were used for ethnoveterinary in the Jammu region, while the lowest number (28%) of species were used in Kashmir. Across the regions, Kashmir and Jammu had the highest level of species similarity (17%), while Jammu and Ladakh had the lowest (1%). A cross-regional assessment of plant resources revealed that 18% of plants were shared among the regions. The reported use of Amaranthus blitum, Morus alba, Ficus palmata, Vitex negundo, Juniperus semiglobosa, Ulmus wallichiana, and Rumex nepalensis are novel for the ethno-veterinary uses of this part of the Himalayan region. The various dry unique traditional fodder preparations (gaaslov, gass khor, pan baath, kaandbaath, Lovgooad, Karb, and Phungma) from plant resources are reported for the first time from the Himalayan region and can be ascribed to the novelty of this study. Plant resources were not only a source of fodder and medicine but also presented themselves as an opportunity for livelihood generation. Therefore, our findings bridge the knowledge gap by documenting key ethnoveterinary applications of native plant species from the study region that are used to cure livestock diseases and disorders by the mountain inhabitants.

Exploring the Status of Preference, Utilization Practices, and Challenges to Consumption of Amaranth in Kenya and Tanzania.

African leafy vegetables such as amaranth have been utilized since time immemorial both as food and as medicine. These vegetables grew naturally in most rural environments, but currently most of them are cultivated both for home consumption and for sale. The aim of this study was to identify the most preferred amaranth species and cooking and utilization practices, as well as the beliefs and attitudes that encourage or discourage use of this vegetable. The study was carried out in seven counties of Kenya and in three regions in Tanzania. Twenty Focus Group Discussions (FGDs) with members of the community and twenty Key Informant Interviews (KIIs) with agricultural and nutrition officers were conducted in the study areas to obtain information on preferred varieties, sources of amaranth vegetables, common cooking methods, alternative uses, beliefs and taboos surrounding amaranth consumption, and the challenges experienced in production and consumption. The findings of the study showed that amaranth is one of the most commonly consumed indigenous vegetables in Kenya and Tanzania. The preference for varieties and cooking habits differs depending on the community and individuals. Amaranthus dubius and Amaranthus blitum were most common in Kenya, while Amaranthus dubius and Amaranthus hypochondriacus were most common in Tanzania. Most people consumed these vegetables because they were affordable and available or because of circumstance of lacking other foods. Regarding cooking, final taste was mostly considered rather than nutritional attribute. Several alternative uses of amaranth such as uses as medicine and livestock feed were also reported, as well as some beliefs and taboos surrounding the vegetable. Training on nutritional attributes and promotion of food preparation practices that ensure maximum nutrient benefits from amaranth is needed at the community level to realize the nutritional importance of the vegetables. Hands-on training and demonstrations were the most preferred modes of passing information.

Comparative Assessment of Free Radical Scavenging, Membrane Stabilizing and Thrombolytic Potentials Of Two Amaranthus Species of Bangladesh

Amaranthus lividus and Amaranthus tricolor were evaluated for their primary free radical scavenging, membrane stabilizing and thrombolytic potentials. The methanol crude extracts of these two species were partitioned into petroleum ether, dichloromethane and aqueous soluble fractions. Antioxidant potential of the extracts was evaluated through DPPH and H2O2 free radical scavenging assays, where the aqueous soluble fraction of A. lividus exhibited the highest activity (p < 0.05). Besides, the maximum level of total phenolics was quantified in the aqueous fraction of A. lividus. Higher level (p < 0.05) of flavonoids (> 70 mg of QE/g of extract) were measured in the aqueous, dichloromethane and crude methanol extracts of A. lividus. The membrane stabilizing (anti-inflammatory) potential of the extracts was studied by measuring the inhibition of hemolysis of RBC induced by hypotonic solution and heat. Considering the conditions of both assays, the aqueous extract of A. lividus showed potent membrane stabilizing activity (p < 0.05) indicating their primary antiinflammatory potential. Very mild thrombolytic activity was noticed for both plants compared to the standard. Furthermore, existence of some major phytochemicals, like alkaloids, steroids, triterpenes and quinones were also observed at various levels. This is the first time report of the comparative assessment of the above mentioned plant extracts so far. Bangladesh J. Bot. 51(1): 139-145, 2022 (March)

A Cross-Cultural Analysis of Plant Resources among Five Ethnic Groups in the Western Himalayan Region of Jammu and Kashmir.

Simple SummaryFor generations, local ethnic communities have amassed a vast body of traditional ecological knowledge (TEK) on the use of plant resources. Ethnobiologists have recently focused on cross-cultural studies in order to record and measure the processes guiding the evolution of TEK within a specific society; both to preserve it and use it sustainably in the future. The current study documents the TEK of plant resources from five ethnic communities of the Jammu and Kashmir (J&K) region, Western Himalayas. Through semi-structured interviews and group discussions, we recorded a total of 127 plant species used by local ethnic groups for various provisioning services (i.e., medicine, food, fodder, fuelwood, herbal tea) and/or with spiritual significance. Across the ethnic groups, Gujjar reported the highest number of plants (25% species), followed by Pahari (24% species), and the lowest number of plants were reported by Dogra (12% species). Looking at plant uses among different cultural groups, we discovered that, especially, some ritual practices were associated with specific plants. We found a relatively high overlap in the use of specific plants among the ethnic groups, namely Gujjar, Bakarwal, and Pahari. Certain species were found to be common in all cultures due to their food value. The current study is a collaborative effort that includes not only documenting, but also cross-cultural comparisons of the documented species, in order to better understand the diverse traditional plant usage systems. This will not only increase regional understanding of cross-cultural ethnobotany, but it will also open opportunities for local people to be rewarded for promoting and celebrating their expertise and participating in future development activities.Plant resources have always been valuable in human life, and many plant species are used in medicine, food, and ritual, and resource utilization is closely related to cultural diversity. Our study was conducted from June 2019 to April 2021, during which we aimed to document the local knowledge of plant resources of five ethnic groups, i.e., the Gujjar, Bakarwal, Kashmiri, Pahari, and Dogra communities of the Jammu and Kashmir (J&K) region, Western Himalayas. Through semi-structured interviews (N = 342) and group discussions (N = 38), we collected data on the ethnobotanical uses of plant resources. The data was subjected to hierarchical cluster analysis and ordination techniques (Principal Component Analysis) via, R software of version 4.0.0. Traditional uses were classified into three groups, i.e., single-, double-, and multi-use groups. The study recorded a total of 127 plant species, belonging to 113 genera and distributed among 64 botanical families. The dominant plant families were the Asteraceae, with 8% of all species, followed by Lamiaceae (6%), Polygonaceae (5%) and Ranunculaceae (4%). The recorded plant taxa were frequently used for medicine (51.4% responses), followed by food (14.9%), and fodder (9.5%). Principal component analysis (PCA) separated three groups of provisioning services depending on plant consumption preference levels. Comparative analysis showed remarkable similarities in plant uses (food, medicinal) among the Gujjar and Bakarwal ethnic groups, as both groups share a common culture. Some plants like Azadirachta indica, Brassica campestris, Ulmus wallichiana, Amaranthus blitum, and Celtis australis were also used for magico-religious purposes. We also recorded some medicinal uses that are new to the ethnobotanical literature of the J&K Himalayas, such as for Betula utilis, Sambucus wightiana, and Dolomiaea macrocephala, in our case for example local medicinal recipe, which is derived from Dolomiaea macrocephala, often known as Nashasta, used to treat weakness, back pain, and joint pain. Similarly, we also recorded new food uses for Eremurus himalaicus. Moreover, we also observed some plants for instance, Fragaria nubicola, Betula utilis and Juniperus communis have spiritual significance (i.e., amulets and scrolls) for this part of the Himalayan region. The present study provides a useful tool for resource management and can help in developing scientifically informed strategies for the conservation of plant resources.

Molecular docking assisted biological functions and phytochemical screening of Amaranthus lividus L. extract

In this study, the phytochemical content of Amaranthus lividus extract and its multi-biological activities were investigated. Total protein, phenol, flavonoid, saponin and condensed tannin contents were determined for phytochemical analysis. In addition, GC–MS and HPLC analyzes were carried out for the determination of the active components of the extract. In determining the multi-biological activities, radical scavenging, anti-mutagenic, anti-proliferative and anti-microbial activities of the extract were investigated. GC–MS analysis revealed that the leaf extract of A. lividus contains phytol and β-sitosterol as major compounds and the presence of gallic acid, caffeic acid, quercetin, vanillin and kaemferol compounds were determined with HPLC analysis. The radical scavenging effect of A. lividus extract was determined as 75.6% against 2,2-diphenyl-1-picrylhydrazyl and 85.2% against superoxide. In anti-bacterial studies, it was determined that A.lividus extract formed different inhibition zones against all tested bacteria. The highest inhibition zone was 14.3 ± 0.7 mm against Bacillus subtilis. In addition, the anti-microbial activity of the extract was demonstrated by molecular docking studies of the binding of gallic acid and phytol to aquaporin and arginase enzyme of bacteria, and the mechanism of anti-microbial activity was explained. A. lividus extract, which provided a 68.59–33.13% reduction in the formation of chromosomal aberrations such as unequal distribution of chromatin, micronucleus formation, fragment, sticky chromosome, bridge and vagrant chromosome, exhibited a strong anti-mutagenic effect. A. lividus extract has a reducing effect on the number of dividing cells and exhibits an anti-proliferative effect of 25.7% compared to the control group. The antiproliferative mechanism of action was investigated by molecular docking and it was determined that the gallic acid and phytol in the extract decreased proliferation by interacting with telomerase. As a result, A.lividus extract consumed as food is a potential natural anti-microbial, anti-oxidant, anti-mutagenic and anti-proliferative source with its rich phytochemical content.

In vitro inhibitory potential of Amaranthus lividus L. against therapeutic target enzymes

Background and Aims: The search for new enzyme inhibitors in plants is attractive because they can be used as drugs in the treatment of various diseases. Amaranthus spp. (Amaranthaceae) includes about 70 different species, some of which are edible and some of which are used in traditional medicine to treat various ailments. Amaranthus lividus L. is a vegetable whose stems and leaves are used for human consumption in Turkey. Methods: In this study, the in vitro enzyme inhibition potential of A. lividus on α-amylase, α-glucosidase, acetylcholinesterase (AChE), elastase, lipase, neuraminidase and tyrosinase was investigated for the first time. For this purpose, water extract of A. lividus was prepared. The tests of enzyme inhibitory activity were carried out by spectrophotometric and fluorometric methods. Results: The water extract showed significant α-glucosidase and α-amylase inhibitory activities. Also, it displayed good elastase, lipase and tyrosinase inhibitory activities. However, it exhibited low inhibitory activity on AChE and neuraminidase. Conclusion: The plant and its active constituents may be used as an antidiabetic enzyme inhibitor with future phytochemical constituent analysis.

Pollen morphology of some species in family Amaranthaceae from Thailand

Abstract. Saensouk S, Saensouk P. 2021. Pollen morphology of some species in family Amaranthaceae from Thailand. Biodiversitas 23: 601-611. The aim of this study was to study the pollen morphology of 14 species, eight genera from the family Amaranthaceae in Thailand by the applied acetolysis method and it was taken under light and scanning electron microcopies. All pollen grains had monad, radial symmetry and apolar. The pollens in this study can be divided into several groups based on shape, size, aperture, mesoporia, the character of aperture, and exine sculpturing. The sizes of the pollen can be divided into three groups, i.e., very small pollen group, small pollen group, and medium pollen group. The shapes of the pollen can be divided into three groups, i.e., spheroidal, pentagon, and dodecahedric. Exine sculpturing can be divided into three groups, i.e., granular, macrogranular, and microspine. The pollen in this study can be divided into five groups based on the character of the aperture, i.e., aperture with convex circular-like shape, aperture with deeply collapsed circular-like shape, aperture with collapsed circular-like shape, aperture with collapsed pentagon-like shape, and 6-angular element or hexagon-like shape. The pollen in this study can be divided into three groups based on the mesoporia, i.e., mesoporia broadly flat, mesoporia moderately and broadly vaulted, and mesoporia narrow and highly vaulted. The number of apertures was found to be between 12-54 apertures per pollen. The aperture diameters of Amaranthaceae pollen in this study ranged from 0.5-5 µm. The exine thickness of the pollen in this study was 1.12±0.04 - 2.14±0.31 µm. The pollen character of this study can be divided into eight types, i.e., Achyranthes-type, Alternanthera-type, Amaranthus-type, Celosia-type, Cyathula-type, Gomphrena-type, Ouret-type, and Psilotrichum-type. The pollen characteristic of Alternanthera pungens Kunth, A. Sessilis (L.) R. BR., Amaranthus blitum subsp. oleraceus (L.) Costea, Cyathula prostrate (L.) Blume., Ouret sanguinolenta (L.) Kuntze, and Psilotrichum ferrugineum (Roxb.) Voigt were reported for the first time.

Health risk assessment for heavy metal accumulation in leafy vegetables grown on tannery effluent contaminated soil

Accumulation of metals (Cr, Zn, Ni, Cd, and Cu) in leafy vegetables cultivated on tannery effluent contaminated soil and agricultural land soil were determined with an Atomic Absorption Spectrophotometer (AAS). The values of risk factors for the human population were studied, where metals were transferred from tannery effluent to plants via effluent contaminated soil and finally, transmitted to human body through the consumption of these metal accumulated leafy vegetables. Leafy vegetables, namely Stem amaranths (Amaranthus lividus), Spinach (Spinacia oleracea), Red amaranths (Amaranthus gangeticus), Jute mallows (Corchorus capsularis), Water spinach (Ipomoea aquatica), and Malabar spinach (Basella alba) were cultivated on the soils collected from downstream of Hazaribagh tannery area and Keraniganj agricultural land. The study revealed that the metal contents in contaminated soil exceeded the permissible limits recommended by WHO/DoE. Tannery effluent contaminated soil was found more polluted than the agricultural land soil. Metal contents in leafy vegetables cultivated on contaminated soil were higher than that of agricultural soil and exceeded the permissible limit, particularly in the case of Cr (125.50–168.99 mg/kg Dw) and Cd (0.19–0.83 mg/kg Dw). Metal content order was found as Cr>Zn>Ni>Cu>Cd for contaminated soil and Zn>Cr>Cu>Ni>Cd for agricultural land soil. The metal accumulation and translocation were found in vegetables in the order of Spinach>Water spinach>Malabar spinach>Jute mallows>Red amaranths>Stem amaranths. The analyses also revealed that the metal translocation rate in the plants of contaminated soil was higher than that of non-contaminated agricultural soil. The values of each risk index exceeded 1 in case of vegetables cultivated in contaminated soil. Therefore, the possible threat of chronic and carcinogenic diseases emerged if those polluted vegetables would be consuming as daily diet.

Analysis of Weed Growth in Rabi Crop Agriculture Using Deep Convolutional Neural Networks

Weed interference for the duration of crop establishment is a severe difficulty for wheat in North India [22.9734 ° N, 78.6569 ° E]. In situ far-flung detection for precision herbicide application minimizes the danger of both crop damage and herbicide input. This research paper focuses on the comparative study of crop growth and its effect at three different places in Madhya Pradesh [24.5840° N, 81.5020° E] India[20.5937° N, 78.9629° E]. These weed species included Pigweed (Amaranthaceae ), Goosefoot [Chenopodiaceae], Wild oat species (Poaceae), livid amaranth (Amaranthus blitum L.), Fathen[Chenopodiaceae (L.) Wild.], and Bermuda grass (Poaceae L.) a significant weed for rabi crop production in India with sensitivity to clopyralid, is the best available put up broadleaf herbicide. The intention of the Takes a look to assess the accuracy of four different CNNs architectures to locate the weed images of the Rabi crop of the family of various Rabi crops growing in competition with Rabi crops at 3 sites in Madhya Pradesh. Four CNNs have been compared, including object detection-primarily based ResNet-50, image classification-based VGGNet-16, Inception v4 and EfficientNet-B7 the EfficientNet-B7 networks have been trained to hit upon both leaves or canopies Everlasting of weeds. Image classification the use of ResNet-50 and VGGNet-16 was largely unsuccessful all through validation with whole pics (Fl-score < 0.04). CNN training elevated the usage of cropped photographs Eternal Broad Fall detection at some stage invalidation for VGGNet (F1-score = 0.77) and ResNet-50 (F1-Score = 0.62). The rabi crop weed leaf-trained inception V4 and EfficientNet-B7 achieved the highest F1-Score (0.94) and F1 Score (0.96) respectively, The aim of leaf-based EfficientNet-B7 extended false positives, even though such errors could be won over with extra training images for network desensitization training. Photograph-based faraway sensing rabi crop will become the most viable CNN test for weeds in competition with the EfficientNet-B7 crop.

Effect of humidity on aflatoxin contamination for selected African leafy vegetables.

In this study, the aflatoxin contamination level of selected freshly harvested and dried African leafy vegetables was investigated after inoculation with Aspergillus flavus spores and incubation at 32%, 74%, 84%, and 96% static relative humidity. The study question was whether Aspergillus sp. growth on selected vegetables: Corchorus olitorius, Crotalaria ochroleuca, Vigna unguiculata, Solanum villosum, and Amaranthus blitum can produce aflatoxins. The experiment was replicated thrice and a control sample was included for each replicate. An Agilent 1260 Infinity HPLC system was used for analysis and we quantified the following aflatoxins; B1, B2, G1, and G2 in the selected vegetables. Our results show that aflatoxin B1, G1, and G2 were all present, with the B1 being prevalent. The contamination level increased with relative humidity increase for both freshly harvested and dried vegetables. However, the dried vegetables had a lower contamination level in comparison with freshly harvested. The findings affirm the importance of post-harvest crop preservation to avoid mycotoxin contamination. The vegetables can suffer aflatoxin contamination when exposed to high moisture and ambient temperature and drying is a suitable method of vegetable preservation.

A centenary for bacterial spot of tomato and pepper.

Disease symptomsSymptoms include water‐soaked areas surrounded by chlorosis turning into necrotic spots on all aerial parts of plants. On tomato fruits, small, water‐soaked, or slightly raised pale‐green spots with greenish‐white halos are formed, ultimately becoming dark brown and slightly sunken with a scabby or wart‐like surface.Host rangeMain and economically important hosts include different types of tomatoes and peppers. Alternative solanaceous and nonsolanaceous hosts include Datura spp., Hyoscyamus spp., Lycium spp., Nicotiana rustica, Physalis spp., Solanum spp., Amaranthus lividus, Emilia fosbergii, Euphorbia heterophylla, Nicandra physaloides, Physalis pubescens, Sida glomerata, and Solanum americanum.Taxonomic status of the pathogenDomain, Bacteria; phylum, Proteobacteria; class, Gammaproteobacteria; order, Xanthomonadales; family, Xanthomonadaceae; genus, Xanthomonas; species, X. euvesicatoria, X. hortorum, X. vesicatoria.Synonyms (nonpreferred scientific names) Bacterium exitiosum, Bacterium vesicatorium, Phytomonas exitiosa, Phytomonas vesicatoria, Pseudomonas exitiosa, Pseudomonas gardneri, Pseudomonas vesicatoria, Xanthomonas axonopodis pv. vesicatoria, Xanthomonas campestris pv. vesicatoria, Xanthomonas cynarae pv. gardneri, Xanthomonas gardneri, Xanthomonas perforans.Microbiological propertiesColonies are gram‐negative, oxidase‐negative, and catalase‐positive and have oxidative metabolism. Pale‐yellow domed circular colonies of 1–2 mm in diameter grow on general culture media.DistributionThe bacteria are widespread in Africa, Brazil, Canada and the USA, Australia, eastern Europe, and south‐east Asia. Occurrence in western Europe is restricted.Phytosanitary categorizationA2 no. 157, EU Annex designation II/A2.EPPO codesXANTEU, XANTGA, XANTPF, XANTVE.