Alytes Cisternasii Research Articles

Immunohistochemical localization of thyrotropic cells during amphibian morphogenesis: A stereological study

TSH-like producing cells have been studied using the peroxidase-antiperoxidase (PAP) immunohistochemical technique and rabbit anti-human thyrotropin (TSH) antiserum in the pars distalis of five species of amphibian anuran tadpoles at different stages of development as well as in 1-year-old postmetamorphic animals. This cell type has been identified and located in the pars distalis ventral zone of Bufo calamita showing no changes in its distribution pattern throughout development, and in Rana perezi where TSH-like cells were located in the central zone of the gland in premetamorphic stages, occupying the ventral zone at the end of metamorphosis. No immunoreaction has been observed in the other species studied ( Hyla meridionalis, Alytes cisternasii, and Pelobates cultripes). Morphometry and stereology were used to evaluate the changes observed in pars distalis volume and TSH immunoreactive cells during development. Pars distalis volume increased during the larval growth period and decreased throughout the metamorphic climax. TSH volume density and cellular area showed different evolutions in the species studied. The correlation between these parameters suggests a greatly proliferative period followed by an increase in cellular size. The changes observed in TSH total volume as well as the physiological data found by other authors lead us to conclude that there are two phases in amphibian development: first a period of TSH storage (pre- and prometamorphosis) and second a period of TSH release at metamorphic climax.

Immunocytochemical and morphometric study of prolactin cells during amphibian morphogenesis

The peroxidase-antiperoxidase (PAP) immunocytochemical technique and rabbit antihuman prolactin (PRL) antiserum were used to localize and identify PRL-producing cells in the pars distalis of Bufo calamita, Hyla meridionalis, Alytes cisternasii, Pelobates cultripes, and Rana perezi tadpoles at different stages of development as well as in 1-year-old postmetamorphic animals. This cell type was located throughout the gland in P. cultripes and R. perezi and in the caudal two-thirds in B. calamita, H. meridionalis, and A. cisternasii in premetamorphic animals. These distribution patterns do not show changes throughout development. Morphometry was used to evaluate the changes observed in pars distalis volume and stereological parameters of PRL immunoreactive cells during development. Pars distalis volume increased during the larval growth period and decreased throughout the metamorphic climax. PRL volume density and cellular area showed different patterns in the different species, although the correlation between these parameters suggests a period of great proliferative rate followed by changes in cellular size. The changes observed in PRL total volume suggest the existence of two phases in amphibian development: (i) a period of PRL storage during pre- and prometamorphosis and (ii) a period of release at the metamorphic climax.