ISEE-749 Objective: This study focuses on examining the relationship between UFP and eNO in 17 nonasthmatic children (9–11 years old) from an elementary school located < 1 km from an ambient air monitoring site in Beijing. Materials and Methods: At this site, a Twin Differential Mobility Particle Sizer System and an Aerosol Particle Sizer were used to measure number concentrations of particles in several size ranges starting from 3 nm and up to 10 μm. Particles were measured at 10-min intervals continuously during the study period (October 2005–April 2006). Each child was measured once per day for 30 days. Exhaled breath samples were collected into an aluminum bag and subsequently analyzed for NO using a chemiluminescent NOx-NO2-NO analyzer. In our analysis, we calculated concentrations of UFP (diameter < 100 nm), both averaged over the 24-hour period before each eNO measurement day and the maximum 1-hour moving average within the same 24-hour period. Linear mixed-effects models were used to regress eNO against UFP, along with or without a copollutant (NO2 or SO2), respectively. A repeated statement was used to estimate the covariance effects of residual; and the covariance structure was chosen as compound symmetry. Results: Results showed that UFP concentrations were 25,484.1 ± 7403 cm−3 and eNO concentrations were 9.7 ± 9.0 ppb. Using 24-hour average concentrations, we found an increase in UFP by 10,000 cm−3 was associated with an increase in eNO by 0.56 ppb (P = 0.14), 0.25 ppb (P = 0.47) after adjusting for NO2, and 0.28 ppb (P = 0.38) after adjusting for SO2. Using maximum 1-hour concentrations, we found an increase in UFP by 10,000 cm−3 was associated with an increase in eNO by 0.22 ppb (P = 0.13), 0.19 ppb (P = 0.15) after adjusting for NO2, and 0.21 ppb (P = 0.09) after adjusting for SO2. Conclusions: These findings suggest a positive association between eNO and UFP. More detailed analyses, considering different particle size ranges, seasonal effects, etc, are being undertaken.
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