Partial melting of the garnet (Grt)–bearing biotite gneiss from the Jianggalesayi area in the southern Altyn Tagh high pressure (HP) – ultra high pressure (UHP) metamorphic belt has been identified and reported here. Felsic veins were distributed along the foliation plane and deformed together with the Grt–bearing biotite gneiss, which dominated the main outcrop and preserved diagnostic features of anataxis. Highly cuspate and irregular quartz (Qz)-melt and plagioclase (Pl)-melt surrounded or stretched into biotite and/or quartz grains with obvious eroded boundaries further provided the microstructural evidences for partial melting of the gneiss. CL images showed that zircons from the Grt–bearing biotite gneiss were characterized by multizone structures of core – Rim I – Rim II – Rim III. The different zircon zones from the gneiss had integrated recordings of the protolith ages 836–1613 Ma; the peak metamorphic age of 503 ± 5 Ma; the retrograded age of 456 ± 4 Ma; and the anatectic age of 418 ± 3 Ma. Zircons from felsic veins also exhibited core – rim structures on CL images. The felsic veins yielded similar protolith ages (1099–1072 Ma) and anatectic age (417 ± 2 Ma) as the host gneiss. The zircons from the host gneiss and felsic veins had plotted identical crustal evolution curves in the εHf(t) vs. U-Pb age diagram with identical TDM ages. The U-Pb ages and Lu-Hf isotope both suggested that the felsic veins were internally derived by the partial melting of the host gneiss. Moreover, the high content of Al2O3 and Rb in felsic veins indicated that the decomposition of biotite accounted for the partial melting of the gneiss. The temperatures calculated by Ti–in–zircon thermometer for the anatectic zircons from the felsic veins (582–694 °C) were identical to the conditions of the amphibolite facies stage of the Grt–bearing biotite gneiss (T = 619–646 °C), which indicated that the crystallization of the melt had taken place during the amphibolite facies stage of the exhumation. The anatectic ages (418 ± 3 Ma and 417 ± 2 Ma) of the gneiss and felsic veins were determined to be within the range of the fourth episode of the early Paleozoic magmatism (420–385 Ma), which had formed in a post-collisional extensional setting. Therefore, the combination of the magmatic heat events and stress release may have played an important role in the partial melting of the gneiss.