Alternative Protein Source Research Articles

Additive effects of Bridelia ferruginea leaf meal as a partial replacement for soybean meal on nutrients digestibility, growth performance, and carcass characteristics of weaner rabbits

BackgroundProtein is a crucial nutrient for enhancing livestock performance and productivity, traditionally sourced from soybean and fish meal. However, issues like erratic availability and rising prices necessitate exploring alternative protein sources. Tropical plants, especially leaf meals, are being considered to replace or supplement conventional protein sources in rabbit diets. Bridelia ferruginea, a plant abundant in West Africa, is recognized for its medicinal properties, but its potential as an animal feed is largely unexplored. This study aims to evaluate the impact of graded levels of Bridelia ferruginea leaf meal (BFLM) as a partial substitute for soybean meal in the diets of weaner rabbits.Materials and methodsThe experiment was conducted at the Animal Science Department of Kwame Nkrumah University of Science and Technology in Ghana. BFLM was obtained by air-drying harvested leaves and milling them. Four diets were formulated, with BFLM partially replacing soybean meal at levels of 0%, 10%, 20%, and 30%. Representative samples of BFLM and all formulated diets were analyzed for their proximate composition and detergent fibre fractions. Twenty-four weaner rabbits were assigned to these diets in a Completely Randomized Design. The study covered a 10-week period, assessing growth performance, nutrient digestibility, carcass characteristics, and economics of production.ResultsThe chemical composition of BFLM revealed favorable attributes for rabbit feeding, with 20.5% crude protein and 92.6% dry matter. Growth performance declined at 30% BFLM inclusion, indicating a possible impact of anti-nutritional factors. Feed conversion ratio increased at higher BFLM levels. Nutrient digestibility showed a decline in dry matter digestibility at 30% BFLM. Carcass characteristics were affected at higher BFLM levels, with dressing percentage decreasing. Weights of internal organs were generally not negatively affected by the inclusion of BFLM. Economic analysis indicated a linear reduction in feed cost with increasing BFLM inclusion.ConclusionThe study suggests that BFLM can be incorporated up to 20% in weaner rabbit diets without compromising productivity, nutrient digestibility, or dressing percentage. Beyond 20%, adverse effects on growth performance and some carcass characteristics were observed. Economically, BFLM incorporation up to 20% lowered feed costs without compromising feed efficiency. Incorporation of BFLM up to 20% in weaner rabbit diets had no adverse effects on the weights and functions of the heart, liver, and kidneys. Overall, BFLM presents a promising alternative protein source for weaner rabbit diets, contributing to cost-effective and sustainable livestock production.

Fermented but Not Irradiated Cottonseed Meal Has the Potential to Partially Substitute Soybean Meal in Broiler Chickens.

This study was conducted to investigate and compare the effects of substituting soybean meal (SBM) with untreated cottonseed meal (CSM), fermented CSM (FCSM), or electron beam-irradiated CSM (ICSM) on the growth performance, cecal microbiota, digestive enzyme activity, apparent ileal digestibility (AID), and excreta gas emission of broiler chickens. A total of 384 one-day-old male broiler chickens were randomly assigned to four experimental diets, with eight replicates per diet and 12 birds per replicate, for six weeks. The experimental diets consisted of a control diet based on corn-SBM and three other diets in which 50% of the SBM (control) was substituted with CSM in its raw, irradiated, and fermented forms. The results showed that throughout the entire rearing period, feeding broiler chickens with ICSM significantly increased average daily gain (ADG) and body weight (BW) compared to the CSM diet (p < 0.05). Replacing 50% of SBM with FCSM led to a significant improvement in BW, ADG, and feed conversion ratio (FCR) compared to the CSM and ICSM diets (p < 0.05). Interestingly, no significant differences in BW, ADG, or FCR were observed between birds fed FCSM and those on the control diet (p > 0.05). Birds fed FCSM diets exhibited the lowest pH value in the crop, ileum, and ceca. Substituting SBM with FCSM significantly reduced Escherichia coli and Clostridium spp. counts in the ceca, while enhancing the presence of Lactobacillus spp. (p < 0.05). The AID of protein and ether extract was higher in the FCSM group than in the CSM and ICSM groups (p < 0.05). Compared to the CSM diet, ICSM feeding improved protein digestibility (p < 0.05). Broiler chickens on the FCSM diet exhibited higher intestinal amylase and protease activity than those on the other diets (p < 0.05). Furthermore, feeding diets containing FCSM significantly reduced ammonia emissions compared to the other diets (p < 0.05). Overall, our results indicated that microbial fermentation of CSM is a more effective approach than irradiation for enhancing the nutritional value of CSM. Therefore, FCSM is recommended as a viable alternative protein source that can safely replace up to 50% of SBM in broiler chicken diets, particularly during times of fluctuating SBM prices and availability issues.

Blood Parameter Response in Growing Alpine Goat Kids Fed Diets Containing Extruded Flaxseed or Pumpkin Seed Cake

Blood parameters can provide information on the nutritional status of goat kids, which is related to both health and performance. The present study aimed to research whether feeding extruded flaxseed (FS) and pumpkin seed cake (PC), as an alternative protein source in diets, has an effect on the hematological and serum biochemical parameters of goat kids during growth. In the small-scale goat farm, 31 French Alpine goat kids aged 32 days were used for the study. The goat kids were subjected to three different feeding treatments: a mixture containing soybean meal and extruded soybeans (CON), a mixture containing 16% PC (PC-16), and a mixture containing 9% FS (FS-9). They were monitored during the suckling, weaning, and post-weaning growth periods. PC-16 and FS-9 in goat kids’ diets did not result in any changes regarding average daily weight gain. The WBC count was higher in goat kids fed FS-9 and PC-16 compared to CON (9.84 and 9.54 vs. 6.61 × 109 L) diets during the weaning period. GGT activity was lowest in the serum of goat kids fed PC-16 compared to CON post-weaning (38.65 vs. 48.40 U/L). In addition, FS-19 increased GPx compared to kids fed PC-16 post-weaning (809.7 vs. 600.8 U/L). Regarding blood parameters, PC-16 and FS-9 can be used in goat kids’ nutrition as alternative sources of proteins on a small-scale goat farm without compromising goat kids’ growth.

Multifunctional properties of peptides derived from black cricket (Gryllus assimilis) and effects of in vitro digestion simulation on their bioactivities

Insects are a rich source of proteins and are produced in systems that have lower environmental impact. As an alternative protein source, they can be consumed directly or used as an ingredient in other formulations. Recently, there has been growing interest in utilizing insect proteins as a substrate to obtain bioactive peptides as well as in investigating the maintenance of their biological properties under physiological conditions. This study aimed to evaluate the impact of simulated digestion on the bioactive properties of protein hydrolysates from black crickets (Gryllus assimilis). Following simulated digestion of the hydrolysate obtained through the application of Flavourzyme, the scavenging activities of ABTS and DPPH radicals, and ferric reducing antioxidant power (FRAP) increased by approximately 17 %, 246 %, and 173 %, respectively. For the hydrolysate obtained using the binary combination of Flavourzyme/Neutrase, the inhibitory activities of α-amylase and α-glucosidase after digestion were 47.87 % and 12.73 %, respectively, not significantly (p > 0.05) different from non-digested hydrolysates. The angiotensin-converting enzyme (ACE) inhibitory activity of the sample hydrolyzed with Flavourzyme/Alcalase proteases was 42.22 %, but this property was completely lost after in vitro digestion. Untargeted proteomic analysis allowed the identification of 22 peptides in the <3 kDa fraction of the digested black cricket protein. The LPPLP sequence was considered potentially bioactive for all activities tested in silico.

Comparative efficacy of amino acid availability and peptidomic analysis of alternative proteins from different sources under dynamic in vitro protein digestion

The increasing global demand for animal protein highlights the critical necessity of exploring alternative protein sources. Accessing the nutritional characteristics of emerging alternative proteins is vital for food processing and consumer acceptance. This study investigated the amino acid availability and peptidomics profiles of plant- (soy), fungal- (filamentous fungus Fusarium venenatum), microalgae- (Nannochloropsis oculata), and insect-based (black soldier fly) proteins using an advanced dynamic in vitro digestion system, with whey protein as a reference. After gastric digestion, soy and mycelial proteins, despite displaying distinct microstructures, exhibited slightly higher protein digestibility levels of 28.79% and 26.36%, respectively, compared to whey protein (22.39%). However, following small intestinal digestion, whey protein (61.31%) significantly outperformed alternative proteins. Mycelial protein (43.71%) exhibited digestibility similar to soy protein (46.12%), markedly surpassing both microalgae protein (33.35%) and insect protein (26.58%). Notably, insect protein achieved an amino acid availability nearly approaching that of whey protein, underscoring its substantial proteolysis capability. Furthermore, microalgae protein produced the highest percentage and number of bioactive peptides under simulated digestion, suggesting potential health benefits such as angiotensin I-converting enzyme (ACE) and dipeptidyl peptidase-IV (DPP-IV) inhibitor activities, which could mitigate the risk of hypertension and Type 2 diabetes. Alternative proteins from different sources demonstrate diverse digestive mechanisms and proteolysis profiles, thereby bolstering their potential applications as nutritious food ingredients in the future.

Industrial processing of chickpeas (Cicer arietinum) for protein production

AbstractThe increasing global interest in plant‐based proteins stems from concerns about the environmental impact, sustainability, animal welfare, and health implications associated with consuming animal‐based proteins. In the frame of alternative protein sources, chickpea (Cicer arietinum) emerged as a rich source of dietary proteins besides containing good amount of carbohydrate, fat, and fiber. As a protein ingredient, chickpea is available in three forms, namely, flour, concentrate, and isolate. This chickpea protein can be extracted using both wet and dry fractionation methods where the former one includes wet extraction followed by isoelectric precipitation, while the later one indicates dry milling followed by air classification. However, different nonthermal emerging technologies have been seen to assist in extracting protein as well as modifying their functionalities. This review gives an outline of the recently available literature on composition, industrial processing and associated technological challenges, functionality, and application of chickpea protein ingredients. Furthermore, discussion on the modification/improvement of chickpea protein functionality with the assistance of emerging technologies and the potentiality of by‐products produced during chickpea protein processing are also included. Based on the available findings and discussion, it is seen that apart from being a comparable source of alternative animal‐based protein to extract, chickpea derived by‐products can also be a potential source of valued ingredients that might contribute to the circular economy.

Harnessing the potential of public procurement for the protein transition – perceived barriers and facilitators

AbstractShifting dietary patterns from animal-based proteins to more plant-based and alternative protein sources – the protein transition – is urgently needed to improve planetary and human health. Public food procurement is considered to be an effective policy instrument to accelerate the protein transition and to be a potential game changer towards a sustainable food system. However, this potential has remained far from leveraged, and it is largely unknown which barriers and enablers exist in that context. Therefore, this study aimed to gain insight into the barriers and facilitators that are perceived by relevant stakeholders when implementing the protein transition in public food procurement. Our study was conducted in the Netherlands, because of the policy goals set by the Dutch government with regard to the protein transition (50% of the proteins consumed should include animal-based proteins and 50% plant-based proteins by 2030) and because the extent to which the protein transition has been included in the food procurement of Dutch (semi-)public organizations is still largely unknown. However, findings are also relevant for other countries. Semi-structured interviews were conducted with participants from a wide range of (semi-)public organizations (e.g., universities, hospitals, governments), in 2022. Five main themes of barriers and facilitators were identified: (1) support and motivation, (2) food availability and offerings, (3) financial considerations and incentives, (4) policies, processes, and contracts, and (5) environmental factors. The insights from this study can strengthen the scientific evidence base and can serve as a foundation for future research. Moreover, the insights can be beneficial to officials working in (semi-)public organizations to effectively design and execute their procurement process, and can help policymakers in policy development to foster (semi-)public organizations to implement the protein transition in their own contexts.

PSI-16 Evaluation of protein quality of defatted black soldier fly (Hermetia illucens) larvae meal compared with traditional protein sources for dogs and cats

Abstract There is increased interest in the use of insects, such as black soldier fly (Hermetia illucens) larvae meal (BSFLM), as an alternative protein source in pet foods. However, additional information is needed to determine how BSFL protein quality compares with traditional pet food protein sources. Thus, the objectives of this study were to determine the chemical composition, amino acid digestibility, and protein quality of defatted BSFLM in comparison with whole egg powder (WEP) and chicken meal (CM) using the precision-fed cecectomized rooster assay. Fifteen cecectomized roosters (n = 5 per substrate) were randomly allocated to the test substrates BSFLM, CM, and WEP. After 26 h of feed withdrawal, 20 g of substrates were crop-fed and excreta were collected for 48 h. Digestible indispensable amino acid score (DIAAS)-like values were calculated using the Association of American Feed Control Officials (AAFCO), The European Pet Food Industry Federation (FEDIAF), and National Research Council (NRC) reference values for different life stages of dogs and cats. Data were analyzed using SAS Proc Mixed 9.4 with P ≤ 0.05 significance. On a dry matter basis, CM had the greatest crude protein (70.2%), followed by BSFLM (59.8%), and WEP (51.2%). In general, WEP had greater amino acid digestibility than BSFLM and CM. BSFLM (84%) and CM (84%) had comparable arginine digestibility, but less than WEP (92%). Lysine digestibility was greater for BSFLM (80%) in contrast with CM (74%), but less than WEP (91%). DIAAS-like values were calculated, revealing methionine + cysteine as the first limiting amino acids for growing puppies and adult dogs in BSFLM according to AAFCO, FEDIAF, and NRC, whereas for CM threonine was the first limiting amino acid based on AAFCO, and phenylalanine + tyrosine based on NRC and FEDIAF. NRC identified methionine and cysteine as limiting for all test substrates in adult dogs. Methionine and cysteine were limiting for growing kittens for BSFLM among AAFCO, FEDIAF, and NRC. For CM and WEP, phenylalanine and tyrosine was the first-limiting amino acid by NRC and FEDIAF. For adult cats, phenylalanine and tyrosine were the limiting amino acid for BSFLMand CM according to AAFCO and FEDIAF, with NRC identifying it as limiting across all protein sources. In summary, methionine and cysteine and/or phenylalanine and tyrosine were the primary limiting amino acids among the protein sources evaluated. In general, BSFLM had comparable indispensable standardized amino acid digestibility to CM.

Impacts of substituting fish meal with full-fat or defatted black soldier fly (Hermetia illucens) larvae on growth, quality, and health of Nile tilapia (Oreochromis niloticus) fingerlings

This study investigated the use of black soldier fly (Hermetia illucens) larvae (BSFL), provided in either full-fat (FBSF) or defatted (DBSF) forms, as an alternative protein source in the diet of Nile tilapia (Oreochromis niloticus) fingerlings. Specifically, the influence was assessed of BSFL on growth performance, hematological parameters, body composition, and histology. Nine diets (including the control) were formulated to be isonitrogenous (35 % crude protein) and isolipidic (6 % crude lipid), relying on fish meal as the primary protein source and gradually replacing it with BSFL meal at levels of 25 %, 50 %, 75 %, or 100 % on an equivalent protein basis, corresponding to dietary inclusion levels of 5.25 %, 10.25 %, 15.5 %, and 20.0 %, respectively. Fish with an initial mean weight ± standard deviation of 1.3 ± 0.26 g were fed for 12 weeks until the fish final weight was in the range 24.08–34.95 g. Based on the results, there were no significant (p>0.05) differences in the growth performance or feed conversion ratios among the treatments. Additionally, there were no significant differences in the hematological responses or the proximate body composition. Furthermore, 100 % substitution of fish meal with FSFL and DBSF meal caused blood congestion in the hepatopancreas region and several intraepithelial leukocytes in the intestine. There were no detrimental impacts at levels of 25 %, 50 %, and 75 % on growth performance, hematological responses, body composition, or the intestines or liver histological characteristics of the O. niloticus fingerlings. This information should provide useful guidance for the management of Nile tilapia, an economically important species.

PSIX-11 Effects of black soldier fly larvae on the serum chemistry, hematology, fecal characteristics, and oral health measures of healthy adult cats

Abstract Black soldier fly larvae (BSFL) serve as an alternative protein source for pet food products. Even though BSFL are one of many insect species being added to dog foods and treats, knowledge of its effects in cats remains limited. Further investigations are imperative to delineate the effects of BSFL-based ingredients in cats. Therefore, the objective of this study was to determine the effects of BSFL on serum chemistry, hematology, fecal characteristics, and oral health measures of adult cats. All procedures were approved by the University of Illinois IACUC prior to experimentation. Adult cats [n = 25; 17 females, 8 males; 6.28 ± 0.27 yr of age; 4.50 ± 0.18 kg body weight (BW)] were used in a completely randomized design. The study was composed of a 21-d baseline phase and a 70-d experimental phase. During the baseline phase, all cats were fed a control diet based on chicken meal (35% of diet). After baseline, cats were randomly assigned to 1 of 2 experimental diets: control diet (n = 12) or BSFL-containing diet (20% whole BSFL meal and 24% chicken meal; n = 13). At baseline, teeth were cleaned by a veterinary dentist. Breath samples were analyzed for volatile sulfur compounds (VSC), salivary pH was measured, and blood samples were collected for serum chemistry and hematology at baseline and after 35 d and 70 d on treatment. At baseline and after 70 d on treatment, fecal samples were scored and collected for pH, dry matter, and metabolite measurement. Gingivitis, plaque, and calculus scores were performed by a veterinary dentist after 70 d on treatment. All data were analyzed using the Mixed Models procedure of SAS. Diet, time, and diet*time interaction effects were tested, with P &amp;lt; 0.05 being considered significant. Over time, VSC increased (P &amp;lt; 0.0001) in all cats, but was unaffected by diet. Diet*time interactions were observed for fecal pH, fecal scores, and blood cholesterol and triglyceride concentrations. Blood cholesterol concentrations increased (P &amp;lt; 0.05) over time in cats fed the control diet, but remained stable in cats fed the BSFL diet. The opposite effect was observed with blood triglycerides. Triglyceride concentrations increased (P &amp;lt; 0.05) in cats fed the BSFL diet over time, but were stable in cats fed the control diet. Cats consuming BSFL had reduced (P &amp;lt; 0.05) fecal pH, dry matter content, and protein catabolite concentrations, but increased (P &amp;lt; 0.05) fecal short-chain fatty acid concentrations and fecal scores. Overall, 20% BSFL dietary inclusion had comparable effects as chicken meal, suggesting that it will serve as a suitable replacement in cat foods.

Effect of green extraction techniques on the quality and functional attributes of protein isolates from cereals and pseudo cereals: a review

SummaryCereals and pseudo cereals (CPC) constitute the main staple diet of the global population. These are great source of carbohydrate, however, ignored as a potential source of protein. Cereals and Pseudo cereals can provide adequate amount of protein if taken in right quantity. Concerns over foods security, nutritional deficiencies, chronic hunger, and sustainability have brought attention to sustainable and alternative sources of protein derived from food and its byproducts. This review explains the extraction of protein from different cereals and pseudo cereals sources, novel extraction techniques, e.g., microwave assisted, ultrasound, pulsed electric field or high‐pressure processing etc., as well as pre‐treatment efficiencies of the recovery of protein, effects of process parameters on the amount of protein extractions, its isolations and possible utilisation in diversified fields. Commercial attempts have been made to extract and use the protein from CPC, but they remain largely underutilised and generally applicable to a small‐scale level. According to the current investigation, protein extraction from CPC is not only an extensive integration of extraction perspectives, process parameters, yield potential and quality, but also should focus on the nutritional aspects, functionality and bioavailability of the separated protein as a desirable dietary component is in the near future.

304 Amino acid digestibility and protein quality of fermented soybean-based ingredients using the precision-fed cecectomized rooster assay

Abstract Fermented soybean-based ingredients may serve as an alternative protein source for pet foods because of their high-quality protein content. The amino acid (AA) content and protein quality of fermented soybean-based ingredients may vary depending on their composition and processing, however, so testing is required. Our objective was to measure the AA composition, AA digestibility, and protein quality of fermented soybean-based ingredients using the precision fed cecectomized rooster assay. The University of Illinois Institutional Animal Care and Use Committee approved all animal procedures prior to experimentation. Cecectomized roosters (n = 36; six roosters per substrate) were randomly allotted to one of five test substrates: 1) autoclaved soybean (ASB); 2) fermented soybeans (FSB); 3) fermented soybeans with probiotics (FSBP); 4) fermented soybean meal (FSBM); 5) fermented soybean meal with probiotics (FSBMP). After 26 h of feed withdrawal, roosters were tube-fed test substrates. Following crop intubation excreta samples were collected 48 h. Endogenous loss corrections for AA were made by using five additional cecectomized roosters. All data were analyzed using SAS version 9.4. All substrates had increased AA digestibilities, with all indispensable AA digestibilities being &amp;gt;80% except for lysine (78.1% to 86.9%). Tryptophan digestibility was less (P &amp;lt; 0.05) in FSB than other substrates, but other AA digestibilities were not different. Digestible indispensable AA score (DIAAS)-like values were calculated to determine protein quality according to nutritional guidelines for adult dogs, adult cats, growing puppies, and growing kittens. The most limiting AA was the same for all ingredients. In adult dogs, methionine was the most limiting AA based on National Research Council (NRC; DIAAS: 33.4-35.1), European Pet Food Industry Nutritional Guidelines (FEDIAF; DIAAS: 49.6-52.1), and Association of American Feed Control Officials (AAFCO; DIAAS: 60.2-63.2) guidelines. In adult cats, phenylalanine was the most limiting AA (DIAAS: 59.5-63.2) when using NRC guidelines, but DIAAS were above 100 for FEDIAF and AAFCO guidelines. In growing puppies, methionine was most limiting according to NRC (DIAAS: 56.7-59.6) and FEDIAF (DIAAS: 78.8-82.7) guidelines, but threonine was most limiting according to AAFCO (DIAAS: 68.8-72.3) guidelines. In growing kittens, methionine was most limiting according to NRC (DIAAS: 70.9-74.4) and AAFCO (53.4-56.0) guidelines, but phenylalanine was most limiting according to FEDIAF (DIAAS: 66.7-70.8) guidelines. Our results demonstrate that fermented soybean-based ingredients are high-quality protein sources. Even though they have one or more limiting AA, they would serve as strong complementary proteins for pet foods. Further research in dogs and cats is necessary to confirm sufficient palatability and digestibility, but these data suggest that they are valuable sources of protein for pet food.

Captive spawning, early development, and larviculture of largescale blackfish, Girella punctata (Gray, 1835), and effects of macroalgae and alternative protein sources on its juvenile growth performance

The largescale blackfish, Girella punctata (Gray, 1835), inhabits rocky substrates in the coastal waters of Japan and northeastern Taiwan. Despite its commercial significance, there is limited knowledge regarding its reproductive biology due to the sparse occurrence of broodstocks in its natural environment. This study investigated the reproductive performance and embryonic development of G. punctata, assesses its feeding preferences through controlled feeding trials, and evaluates the impact of various feed types on its growth performance. In total, 124 wild-caught G. punctata were reared for 3–4 years, and their reproductive performance was assessed in 2015 and 2016. Their egg production increased with a sudden water temperature change from 17 ℃ in 2015–22 ℃ and 2016. The total and daily average egg production weights were 3589.22 and 40.79 g, respectively, whereas the total and daily average fertilized egg production weights were 1630.72 and 18.59 g, respectively; moreover, the hatching rate was 42.61 %. During embryonic development, the eggs hatched 45 h post fertilization at 19 ℃. Newly hatched larvae were 2.26 ± 0.04 mm in length with a yolk sac of 1.09 ± 0.02 mm in length. The yolk sac was almost completely absorbed 3 days post hatch (dph), when the larvae grew to a length of 3.42 ± 0.06 mm. We found that fish meal with 40 % protein was the optimal G. punctata fish diet. The weight gain in juveniles fed Cladophora fascicularis ranged from 47 % to 52 %, which was significantly higher than that in those fed Hypnea charoides (p < 0.05). The present study provided a practical guideline on the conditions required for successful fertilization and early development of G. punctata embryos and other larval stages. This information should help ensure greater survival and a higher proportion of healthy G. punctata larvae for mariculture applications.

Which Novel Ingredient Should be Considered the “Holy Grail” for Sustainable Production of Finfish Aquafeeds?

ABSTRACTThe immense production of fishmeal and fish oil is dramatically intensifying the severe state of pelagic fisheries overexploitation. The latter in conjunction with the increasing demand for low‐cost protein‐rich food supply prompt aquaculture to employ new practice. Several novel dietary ingredients are currently under evaluation for potential incorporation in aquafeeds in an effort to shift the aquaculture sector toward a more sustainable and economic production. The present review aims to summarize the existing findings regarding the effects of studied alternatives to fishmeal and fish oil on the most valuable and commercially produced marine (Sparus aurata and Dicentrarchus labrax) and freshwater (Salmo salar and Oncorhynchus mykiss) finfish species in European aquaculture. Alternative protein sources, including macroalage (marine plants), krill (marine fishery), insects (terrestrial), terrestrial animal by‐products (processed/rendered), and single cell ingredient (biotechnology), are discussed for their efficiency in promoting the growth and the welfare of both fry and adult cultured finfish species. Applicability of these ingredients is reviewed in terms of nutrient composition, dietary inclusion level, performance output, digestibility, and health benefits. In addition, a meta‐analysis was conducted based on data from peer‐reviewed scientific publications in order to assess whether novel ingredients meet the dietary protein (amino acid) and lipid requirements of finfishes. Aquafeed reformulation strategies should ensure the recommended daily nutritional requirements and additionally indicate the meta‐analysis alternatives, such as microalgae, which are deficient in essential amino acids. The sustainable expansion of aquaculture is on the horizon, but which novel ingredients may be regarded as the key drivers to its establishment?

Partitioning of proteins and small molecular weight compounds from alfalfa juice during ultrafiltration

Ribulose-1,5-biphosphate-carboxylase/oxygenase (RuBisCO) from alfalfa is a promising source of alternative food protein, but it is challenging to purify. Ultrafiltration shows potential for concentrating and fractionating RuBisCO from low molecular weight compounds present in the extracted juice, but there is limited information on their partitioning depending on the membrane pore size. Therefore, the objective of this study was to evaluate the partitioning of various compounds from the juice depending on membrane pore size. It was hypothesized that both membrane pore sizes will retain RuBisCO but might partition low molecular weight compounds within the matrix differently. RuBisCO was fully retained by both the 300 and 100 kDa polyethersulfone (PES) membranes, while ash (29.7%–35.4% dry matter (DM)), non-protein nitrogen (2.38%–2.53% DM), and free N-terminals (10.13–10.34 mg glutamic equivalent/100 mg DM) were transmitted through both membranes. The retentates showed a distinct yellow color when filtering with 100 kDa membranes, but not when using 300 kDa membranes. Although no significant differences in total polyphenol permeation were observed through a spectrophotometric assay, a metabolomics screening analysis showed that the 100 kDa membranes had less transmission of the flavonoids apigenin, chrysoeriol, tricin, and tricin glycosides, which might contribute to the yellow color. These results reiterate the importance of measuring individual polyphenols using hyphenated techniques as opposed to spectrophotometric total reducing capacity. Sixteen saponins were identified and their permeation was lower in the 100 than the 300 kDa membranes, indicating a better potential for higher degree of protein refinement for the latter. This study demonstrates for the first time the difference in partitioning of the small molecular weight components, using PES membranes of similar chemistry but different size, during ultrafiltration of alfalfa juice.

Understanding the Potential of Microbial Protein as a more Sustainable Food Source

Abstract The increasing global demand for protein, driven by population growth and dietary shifts, poses a challenge to conventional, inefficient food production systems. In response, alternative protein sources like microbial protein have gained traction for their potential to supply nutritional food more sustainably. Microbial protein, the protein-rich biomass of microorganisms, offers a promising solution due to its high nutritional value, resource efficiency, and climate resilience. However, challenges persist in transitioning to the use of sustainable feedstocks and navigating complex legislative frameworks, hindering widespread adoption. Effective legislative reforms, streamlined regulatory processes, and technological innovations are essential to unlock the full potential of microbial protein as a more sustainable food source. Moreover, consumer acceptance and behavioral changes are crucial for driving adoption and harnessing the environmental benefits of microbial protein. Ultimately, collaborative efforts across stakeholders, informed consumer choices, and policy interventions are imperative for realizing a sustainable food future, with microbial protein playing a pivotal role in this transformative journey. Information © The Authors 2024

Consumer's Cultured Meat Perception and Acceptance Determinants: A Systematic Review and Future Research Agenda

ABSTRACTThe reliance on animal husbandry for protein has caused environmental, social and ethical challenges worldwide, yet the demand for animal‐based food continues to rise. Despite the increasing attention from scholars and professionals on the potential of cultured meat (CM) as a viable alternative protein source, the ensuing scholarly work still lacks coherence and is incomplete and fragmented. Its success also depends heavily on customer acceptance. This study therefore examined peer‐reviewed literature on consumers' perceptions and acceptance of CM. A comprehensive search was conducted on ScienceDirect, Google Scholar and Scopus databases between 2017 and 2022, yielding 54 papers that met the selection criteria. The qualitative synthesis conducted revealed seven themes that had the highest influence on consumers' acceptance of CM. The themes identified were (i) attitudes and traits, (ii) situational impact, (iii) information and nomenclature, (iv) CM properties, (v) risk–benefit perception, (vi) familiarity and awareness and (vii) competition with other alternative proteins. This review also identified demographic predictors of consumers who are most likely to accept CM. The review found that although consumers are curious and willing to try CM, failure to address personal attitudes and traits may lead to failure in adoption. It was also found that strategies that include emotional responses are more effective in the long run.

Impacts of industrial food wastes on nutritional value of mealworm (Tenebrio molitor) and its gut microbiota community shift

The extensive investigation into the capacity of mealworms to digest diverse food by-products, as well as plastic wastes, has been a focal point in recent years. The transition from traditional diet sources like brans to food wastes has the potential to impact the physiological properties of mealworms. This study explored the utilization of various industrial food wastes such as okara, barley spent grain (BSG), sesame oil meal (SOM), and spent coffee grounds (SCG) as feed alternatives, and reports on their survival rate, biomass variations, and nutritional composition. In additional, the shift in their gut microbiota was also assessed. Among the range of industrial food wastes, mealworms exhibited the most robust growth performance when nourished with BSG. This particular group showed a survival rate of 98.33 % and a biomass increase of 23.06 %. In contrast, mealworms fed with SCG demonstrated the lowest survival rate and experienced a significant reduction in biomass. Although the groups fed with okara and SCG displayed moderate growth performance, both exhibited protein levels comparable to those observed in the oatmeal-fed group (used as the positive control). Notably, the inclusion of BSG in the mealworm diet exhibited the potential to enrich their omega-3 fatty acid content, suggesting potential benefits for applications as animal feed or even human consumption. Furthermore, an analysis of the gut microbiome was conducted to investigate the associations between specific diets and the composition of mealworm gut microbiota. In summary, food wastes such as BSG may be repurposed as feed substrates for mealworms before converting them into an alternative source of protein.

Production and toxicological analysis of a powdered beverage based on Brazil nut milk and acai

Considered one of the main seeds in the Amazon region, the Brazil nut (Bertholletia excelsa) has a very high nutritional and economic potential, being rich in minerals and serving as an alternative source of proteins. Allied to it, there is açaí (Euterpe oleracea), an extremely energetic Amazonian fruit, rich in anthocyanins with antioxidant power and with several benefits for the body. In this context, the project aimed to combine these beneficial characteristics of these two foods to create a healthy product with high energy potential, also carrying out its toxicological analysis, measuring the level of selenium and the presence of aflatoxins at the end of the process. It was then observed that the produced product showed potentially positive results, however, specific legislation for these functional beverages is necessary .

Towards a Comprehensive Definition of Pandemics and Strategies for Prevention: A Historical Review and Future Perspectives.

The lack of a universally accepted definition of a pandemic hinders a comprehensive understanding of and effective response to these global health crises. Current definitions often lack quantitative criteria, rendering them vague and limiting their utility. Here, we propose a refined definition that considers the likelihood of susceptible individuals contracting an infectious disease that culminates in widespread global transmission, increased morbidity and mortality, and profound societal, economic, and political consequences. Applying this definition retrospectively, we identify 22 pandemics that occurred between 165 and 2024 AD and were caused by a variety of diseases, including smallpox (Antonine and American), plague (Justinian, Black Death, and Third Plague), cholera (seven pandemics), influenza (two Russian, Spanish, Asian, Hong Kong, and swine), AIDS, and coronaviruses (SARS, MERS, and COVID-19). This work presents a comprehensive analysis of past pandemics caused by both emerging and re-emerging pathogens, along with their epidemiological characteristics, societal impact, and evolution of public health responses. We also highlight the need for proactive measures to reduce the risk of future pandemics. These strategies include prioritizing surveillance of emerging zoonotic pathogens, conserving biodiversity to counter wildlife trafficking, and minimizing the potential for zoonotic spillover events. In addition, interventions such as promoting alternative protein sources, enforcing the closure of live animal markets in biodiversity-rich regions, and fostering global collaboration among diverse stakeholders are critical to preventing future pandemics. Crucially, improving wildlife surveillance systems will require the concerted efforts of local, national and international entities, including laboratories, field researchers, wildlife conservationists, government agencies and other stakeholders. By fostering collaborative networks and establishing robust biorepositories, we can strengthen our collective capacity to detect, monitor, and mitigate the emergence and transmission of zoonotic pathogens.