Alternative Production Research Articles

Public acceptance of beef carbon tax earmarks

The escalating effects of climate change make it important to find ways to offset the carbon burden. One way to do so is to reduce beef consumption, since cattle production creates large amounts of greenhouse gases, which contribute to global warming and climate change. In this study, we explore US consumers’ perceptions of alternative beef products. Specifically, we test the effects of three types of earmark information on consumers’ valuation of a beef carbon tax related to hamburger meat through two labeled discrete choice experiments. Our results show that earmark information can be effective in reducing the negative impact of an environmental tax on consumers’ willingness to pay for beef products. This study contributes to a better understanding of how consumers would bear environmental taxes for beef and alternative products.

Towards better understanding the economic and environmental sustainability of alternative agricultural cropping production systems through integrated modelling

We explore and develop an integrated model to enhance understanding and evaluation of the economic and environmental sustainability performance of alternative agricultural cropping production systems. More specifically, our developed Environmentally Sustainable Residual Income Decomposition Modelling (ESRIDM) integrates accounting decomposition analysis, Water and Economic Sustainability Performance Measurement (WESM), system thinking, modular thinking, Environmentally Sustainable Residual Income (ESRI) theory, into a holistic modelling system influenced by Life Cycle Assessment (LCA) and Life Cycle Costing (LCC) approaches.The ESRIDM is theorised and applied in the context of nitrogen management in Australian Cotton production through a detailed case study and comparative analysis of three nitrogen fertiliser options. We find the ESRIDM is able to: (i) provide an overall assessment of the economic and environmental sustainability performance of each scenario modelled; (ii) increase the transparency of the production system to enable the identification of key issues which influence the likely suitability of each scenario; (iii) enable an analysis of probable outcomes under various alternatives; (iv) evaluate the feasibility of regulatory option modelled. While this paper offers a novel perspective to a specific context, ESRIDM is designed to be adapted to a variety of contexts with a view to contributing to the evolution of more sustainable systems of production more generally.

Selecting the most successfull recycling strategy over daily consumption products: application of q-Rung Orthopair Fuzzy Topsis method

Recycling is the process of collecting and reusing that helps the countries to achieve their sustainable development goals. This study, for the first time in the literature, considers the recycling of many daily consumption products as a decision-making problem with the q-rung orthopair fuzzy (q-ROF) approach. In Turkey, recycling initiatives are primarily led by the government and municipalities, involving either reprocessing in public facilities or collaboration with private enterprises. The research evaluates the effectiveness of recycling strategies, considering paper, plastic, textiles, batteries, frying oils, electronics, glass, and wood as alternative products. Criteria such as convertibility rate, resource usage for recycling, converted product lifespan, recycling process complexity, economic gain, product consumption rate, and trading opportunities are employed in the decision-making process. The q-rung orthopair fuzzy Technique for Order Preference by Similarity to Ideal Solution (q-ROFTOPSIS) method is applied to assess these criteria. Decision makers, comprising a recycling expert, a recycling business engineer, and an academician specializing in recycling studies, contribute to the evaluation. The study reveals electronic products as the most successful in recycling, while frying oils exhibit the least success.

Vegan Diet: A Novel Trend in Healthy Living

Abstract: An entirely animal-free diet that prioritizes natural plant-origin foods such as vegetables, fruits, whole grains, pulses, and lentils is known as a vegan diet. Lowering persistent diseases like type-2 diabetes, cardiovascular conditions, cancer, and many others offers numerous positive health effects. Different aspects of how a vegan diet affects health are studied, and the dietary pattern is analyzed. Along with the trend of a vegan diet, many people have become aware of the importance of following a vegan diet, and many do this for health reasons or due to religious beliefs. A vegan diet has also been seen to positively affect aging. As vegan diet choices are growing, there are now more options for meat and non-dairy alternatives. Optimization for developing an alternative vegan food product is necessary to produce the most favorable product quality and achieve the best. This paper indicates the vegan diet as a whole and how the vegan diet can help treat chronic diseases. It also reviews vegan products for alternative use and their stance in the food industry.

Optimization of torrefaction conditions on antioxidant activity of prickly pear seeds extract using response surface methodology and chemometric analysis

The prickly pear (Opuntia ficus-indica L., Cactaceae) seeds can be used as an alternative food product by the torrefaction process. Torrefaction is a pre-treatment process of biomass into solid fuel within a temperature range of 60-200°C. This study aimed to investigate the effect of torrefaction parameters on the antioxidant potential of prickly pear seeds, which is considered a food by-product, by using the Central Composite Design approach (CCD).The best torrefaction parameters found to have the optimal phytochemical contents were: 200°C and 50 min with 104.86±1.94 GAE/g extract for Total Phenolic Content (TPC), 200°C and 50 min with 81.23±0.90mg QE/g extract for Total Flavonoid Content (TFC), 200°C and 50 min with IC50=90.66±2.09 μg/ml for free radical scavenging activity (DPPH assay), 200°C and 50 min with IC50=124.9±4 μg/ml for radical cation total antioxidant capacity (ABTS assay). Our results demonstrated an increase of antioxidant activities with the increase of torrefaction parameters. Therefore, torrefaction has proven to be a value-added process to exploit prickly pear seed biomass and use it as a source of antioxidant food additives.

Eco-friendly drugs induce cellular changes in colistin-resistant bacteria

Abstract Background and objective As a last option, multidrug-resistant Gram-negative infections (caused by Enterobacteriaceae) are treated with the antibiotic colistin, also known as polymyxin E. Colistin-resistant superbugs predispose people to untreatable infections, possibly leading to a high mortality rate. This project aims to study the effect of Acacia nilotica aqueous extract and zinc oxide nanoparticles (ZnO-NPs) on colistin-resistant Klebsiella pneumonia (CRKP). Materials and methods ZnO-NPs were synthesized using the green method and characterized by UV-vis, Fourier transform infrared spectroscopy, and X-ray diffraction. The zone of inhibition (ZI) was measured using the agar-well diffusion method, and the minimum inhibitory concentration (MIC) and minimum bactericidal concentration were estimated to determine the antimicrobial activity of the tested compound. Scanning electron microscopy (SEM) and transmission electronic microscopy (TEM) were used to investigate the alterations in bacterial cells that were treated with the tested drugs. Results The synthesized ZnO-NPs presented good chemical and physical properties, and the plant extract and ZnO-NPs displayed a large ZI. ZnO-NPs had the lowest MIC (0.2 mg·mL−1). SEM and TEM observations revealed various morphological modifications in CRKP cells, including cell shrinkage, cell damage, cytoplasm loss, cell wall thinning, and cell death. Conclusion A. nilotica aqueous extract and ZnO-NPs could be used as alternative natural products to produce antibacterial drugs and to prevent CRKP infection.

Alternative Production of Hydrocarbons from Natural Gas (Methane) via Synthesis Gas and Methanol

Abstract As a result of research, it has been found that DME from natural gas (methane), oil production and oil refinery wastes and low molecular weight olefins based on it are studied. Therefore, methanol and DME, along with synthesis gas, are relevant for the improvement of the production technology of environmentally friendly motor fuels. The most active and selective catalysts for the dehydration of methanol in DME have a texture that allows the resulting DME to desorb from the surface without steric hindrance. Such “open surface” catalysts of acidic nature include mesoporous γ - Al2O3 and large-pore zeolite HU, whose promotion with H3PO4 achieves 100 % selectivity for DME formation at 88-90 % conversion of methanol. Experiments were performed by varying the amount of methanol in the CH3OH : (CO + H2) reaction mixture from 25 to 100 % by volume. In the main experiments with CO : H2 = 1:1 ratio, the linear speed of the reaction gas flow was kept constant at 3.8 ÷ 0.2 cm3 min−1, that is, the contact time was kept constant for 15.5-16 s. The reaction products were analyzed by gas chromatography.

Economic optimization of sustainable soil management: a Dutch case study

Soil quality is pivotal for crop productivity and the environmental quality of agricultural ecosystems. Achieving sufficient yearly income and long-term farm continuity are key goals for farmers, making sustainable soil management an economic challenge. Existing bio-economic models often inadequately address soil quality. In this study, we apply the novel FARManalytics model, which integrates chemical, physical, and biological indicators of soil quality indicator, quantitative rules on how these indicators respond to farmers’ production management over time, and an economic calculation framework that accurately calculates the contribution of production management decisions towards farm income. This is the first study applying this model on existing arable farms. FARManalytics optimizes crop rotation design, cover crops, manure and fertilizer application and crop residue management. Nine Dutch arable farms were analyzed with a high variation in farm size, soil type, and cultivated crops. First, we assessed farm differences in soil quality and farm economics. Second, we optimized production management to maximize farm income while meeting soil quality targets using farm-specific scenarios. Third, we explored the impact of recent policy measures to preserve water quality and to increase the contribution of local protein production. The results show that the case farms already perform well regarding soil quality, with 75% of the soil quality indicators above critical levels. The main soil quality bottlenecks are subsoil compaction and soil organic matter input. We show that even in front-runner farms, bio-economic modeling with FARManalytics substantially improves economic performance while increasing soil quality. We found that farm income could be increased by up to €704 ha−1 year−1 while meeting soil quality targets. Additionally, we show that to anticipate on stricter water quality regulation and market shift for protein crops, FARManalytics is able to provide alternative production management strategies that ensure the highest farm income while preserving soil quality for a set of heterogenous farms.

Changes in the biological and nutritional value of pea and bean grains as a result of their bioactivation

The relevance of the study is due to the need to improve the technology for processing peas and beans of domestic selection into plant-based drinks and their use in technologies for alternative products of complex raw materials for healthy, functional and specialized nutrition of various population groups. The purpose of the work is to study the influence of the developed technological modes of bioactivation on the nutritional and biological value, functional and technological properties of the protein-carbohydrate complex of pea and bean grains. The work examines the results of studies of changes occurring in pea grains bred at the Bashkir Research Institute of Agriculture and beans bred at the Omsk State Agrarian University during their bioactivation using the Smart Sprouter "Rosinka" installation, in particular the biochemical composition and inhibitory activity of grain of the studied varieties, a protein complex and amino acid composition, protein digestibility in vitro, functional and technological properties of the protein-carbohydrate complex of grain. The research has been carried out using generally accepted, modern instrumental methods for analyzing the properties of raw materials and finished products. It has been established that the use of the developed technological parameters of bioactivation leads to modification of the functional and technological properties of the structure-forming nutrients of grain, intensive accumulation of all amino acids, with the exception of threonine, make it possible to increase the protein content in pea grain by 15.32 % and beans by 14.96 %, relative to the initial values increase the degree of compliance of pea and bean grain protein with the FAO/WHO reference protein by 4.48 and 17.53 %, reduce the activity of grain protease inhibitors by 19.76 and 26.12 %, increase the digestibility of grain protein by 7.77 and 5.85 %. The research results prove the feasibility of using bioactivated pea and bean grains for the production of alternative products with a complex raw material composition, including plant-based drinks and products in the "dairy alternatives" segment.

Environmental Impact Assessment of a Plant Cell-Based Bio-Manufacturing Process for Producing Plant Natural Product Ingredients

Purpose: This study employed a Life Cycle Assessment (LCA) methodology to evaluate the environmental impacts of a novel plant cell-based biomanufacturing process for producing plant natural product ingredients. The primary purpose was to assess the relative sustainability of the process and to provide insights into potential areas of improvement in the biomanufacturing process. Method: The LCA method used an MS Excel (Ver. 2407) -based approach with a cradle-to-gate system boundary covering raw material sourcing (A1), raw material transportation (A2), and product extract manufacturing (A3) stages. Energy use and material inventory data are presented for different unit operations, and environmental impact factors were obtained from the Ecoinvent database. The study included a Material Circularity Index (MCI) calculation to assess the circularity of the biomanufacturing process for the production of saponin emulsifiers that are normally extracted from the woody tissue of the Chilean soapbark tree (Quillaja saponaria). Comparative analyses were performed against a wild-harvest approach for plant tannin extraction from spruce (Picea abies) tree bark. Key Results: The environmental impact assessment focused on determining relative Global Warming Potential (GWP), Acidification Potential (AP), Freshwater Eutrophication (FE), Particulate Matter Formation (PMF), and Ozone Depletion Potential (ODP). Results indicated that the extract manufacturing stage (A3) contributed significantly to adverse environmental impacts, with varying levels of effects based on the energy source used. Comparative analysis with the wild harvest approach highlights the lower environmental impact of the alternative biomanufacturing process. The biomanufacturing process showed a 23% reduction in GWP, AP, and FE and a 25% reduction in PMF and ODP relative to the wild harvest approach. However, the MCI for the biomanufacturing process was estimated to be 0.186, indicating a low material circularity. Conclusions: The results revealed that the extract manufacturing stage, particularly energy consumption, significantly influences the relative environmental impacts of the alternative production processes. Different energy sources exhibit varying effects, with renewable energy sources showing lower environmental impacts. The Material Circularity Index indicated a low circularity for the biomanufacturing process, suggesting opportunities for improvement, such as incorporating recycled or reused materials. Compared with the tannin extraction process, the plant cell-based biomanufacturing process demonstrated lower environmental impacts, emphasising the importance of sustainable practices and the use of renewable energy sources in future plant natural product sourcing. Recommendations include implementing more sustainable practices, optimising raw material choices, and extending product life spans to enhance circularity and overall environmental benefits.

한국의 e커머스 시장 전략방향 연구: Business Model Canvas, 5Force Model을 이용한 공급망 전략 측면

Purpose: This study derives a sustainable strategic direction for K-commerce companies to respond to a threatened entry of multinational companies, mainly Chinese (C-commerce) companies, into the Korean e-commerce market. Research design, data, and methodology: The methodology includes a market approach, the Business Model Canvas, and the 5Force Model for the study period from March to July 2024. Results: The direction of Korea's e-commerce market success strategy, classified into asset type and non-asset type, is that the first is One Strength, which has the competitiveness and entry power to lead existing competitors. The second is that One Medle has the bargaining power of suppliers that can protect existing customers from conversion due to consumer benefits and seller benefits. The third is Three Weakness, which is facing the threat of new entrants, the bargaining power of buyers, and the threat of alternative products, so there are always risks. Conclusions: The implications of the “One Strength, One Medle, and Three Weakness” sector derived from Korea's e-commerce market strategic direction study are summarized into three in terms of supply chain strategy and presented in the text.

The Effects of Smoking on Dental Implant Failure: A Current Literature Update.

Background: This systematic review assesses the current literature (2020-2024) evaluating the impact of smoking on dental implant failure rates. Methods: A non-funded Pubmed database review was conducted according to PRISMA guidelines, and the results were tabulated to extract the study design, patient characteristics, follow-up time, comparison, outcome, and strengths and weaknesses, including risk of bias. This review included 33 studies with 29,519 implants placed in over 18,301 patients. We included prospective and retrospective clinical studies, randomized and non-randomized controlled trials, cohort studies, and observational studies that examined smoking's effects on implant failure rates. Studies had to classify individuals into two groups, smokers and non-smokers, with at least ten implants. Exclusions included reviews, case reports, experimental studies, guidelines, non-English publications, studies lacking comparative data on failure rates, those excluding smokers, and studies focusing on head and neck cancer patients or specialized implants. Results: Our findings indicate a significant correlation in 25 out of 33 studies between smoking and increased implant failure rates, affecting both early and late stages of implant integration and survival as well as revealing a dose-response relationship, with higher daily cigarette consumption significantly increasing the risk of implant failure. Conclusions: This review highlights the importance of smoking cessation efforts, patient education, and tailored patient care in dental implantology. Future research should explore the effects of smoking frequency and alternative tobacco products, such as e-cigarettes, aiming to improve success rates among smokers.

Combinatorial optimization of the hybrid cellulase complex structure designed from modular libraries.

Cellulase selectively recognizes cellulose surfaces and cleaves their β-1,4-glycosidic bonds. Combining hydrolysis using cellulase and fermentation can produce alternative fuels and chemical products. However, anaerobic bacteria produce only low levels of highly active cellulase complexes so-called cellulosomes. Therefore, we designed hybrid cellulase complexes from 49 biotinylated catalytic domain (CD) and 30 biotinylated cellulose-binding domain (CBD) libraries on streptavidin-conjugated nanoparticles to enhance cellulose hydrolysis by mimicking the cellulosome structure. The hybrid cellulase complex, incorporating both native CD and CBD, significantly improved reducing sugar production from cellulose compared to free native modular enzymes. The optimal CBD for each hybrid cellulase complex differed from that of the native enzyme. The most effective hybrid cellulase complex was observed with the combination of CD6-4 from Thermobifida fusca YX and CBD46 from the Bacillus halodurans C-125. The hybrid cellulase complex/CD6-4-CBD46 and -CD6-4-CBD2-5 combinations showed increased reducing sugar production. Similar results were also observed in microcrystalline cellulose degradation. Furthermore, clustering on nanoparticles enhanced enzyme thermostability. Our results demonstrate that hybrid cellulase complex structures improve enzyme function through synergistic effects and extend the lifespan of the enzyme.

Effects of Mela Organics' New Liquid Fertilizer on Tomato Yield in Semi-Arid Areas of Ethiopia

Soil organic matter depletion is mainly caused by crop residue removal, and low or no application of organic fertilizers often results in nutrient exhaustion and low crop yields. This challenge requires the evaluation of different organic fertilizer resources to provide new alternative fertilizer products to an Ethiopian production system. Based on this fact, an experiment was conducted to evaluate the effects of mela organic fertilizer as a supplementary fertilizer to improve the yield of tomatoes under irrigation in the Central Rift Valley of Ethiopia from 2020 to 2021. The experiment consisted of eight treatments (Control, 100% recommended fertilizer, 100% recommended fertilizer + 30 L/ha Mela, 100% recommended fertilizer + 45 L/ha Mela, 75% recommended fertilizer +30 L/ha Mela, 75% recommended fertilizer + 45 L/ha Mela, 50% recommended fertilizer +30 L/ha Mela and 50% recommended fertilizer + 45 L/ha Mela) laid out in randomized complete block design with three replications. The results revealed that the highest agronomic yield was obtained by applying the 100% recommended fertilizer + 30 L/ha Mela. However, based on the partial budget analysis, the highest marginal rate of return was obtained from 50% recommended fertilizer + 45 L/ha Mela. Thus, it can be concluded that the application of Mela Organics up to 45 L/ha, integrated with chemical fertilizer applications, can increase tomatoes' economic yields and reduce fertilizer costs.

Retail Loans for Residential Solar in India: An Alternative Financial Product

Retail Loans for Residential Solar in India: An Alternative Financial Product

An In-Depth Analysis of the Influence of Pharmaceutical Marketing Strategies on the Prescription Practices and Decision-Making Processes of Medical Professionals.

The study seeks to explore how pharmaceutical marketing strategies impact doctors’ prescribing habits within the complex relationships between healthcare providers and pharmaceutical companies. Five alternative pharmaceutical products are analyzed across six distinct evaluation parameters to understand the multifaceted impact of marketing efforts. The dataset includes information on marketing spend, prescription rates, and various evaluation criteria such as efficacy, safety, affordability, brand recognition, promotional materials, reputation, and convenience. These parameters are crucial in assessing the comprehensive influence of pharmaceutical marketing on doctors’ decision-making processes. Understanding how pharmaceutical marketing impacts prescription practices is crucial for ensuring patient welfare and safety. It helps in evaluating whether prescriptions are driven by genuine medical needs or influenced by marketing strategies, thus promoting patient-centered care. The study addresses ethical concerns related to the interactions between pharmaceutical companies and healthcare professionals. Uncovering the extent of marketing influence aids in developing ethical guidelines and regulations, fostering transparency and trust in the healthcare system. The COPRAS-G method necessitates identifying selection criteria, evaluating relevant information for these criteria, and creating methods to assess how well the surrogate meets the participants’ needs. Decision analysis involves a Decision Maker (DM) who must consider a specific set of alternatives and choose one from several options, often with conflicting criteria. Consequently, the developed complexity proportionality assessment (COPRAS) method can be applied. The results indicate that Doctor 03 ranked first, while Doctor 05 ranked the lowest.

Blastocystis species growth inhibition in vitro by plant extracts

BackgroundThe protist Blastocystis species (sp.) inhabits the gastrointestinal tracts of humans and animals. In recent decades, alternative natural products derived from plants have demonstrated potential as effective treatments for Blastocystis infection. The anti-Blastocystis activity of three herbal ethanolic extracts— Odontites linkii subsp. cyprius, Ptilostemon chamaepeuce subsp. cyprius and Quercus alnifolia—were investigated in this study. MethodsThree distinct isolates of Blastocystis sp. maintained in vitro were molecularly subtyped. Cytotoxicity analysis was performed on individual Blastocystis sp. isolates using 250, 500, 1000, and 2000 μg/mL herbal ethanolic extracts for 24 and 48 hours. Quantitative, morphological, and size alterations of Blastocystis cells assessed the cytotoxicity of herbal anti-Blastocystis effect. ResultsFollowing subtyping analysis, one strain of Blastocystis had ST3 and ST1 mixed subtypes, and two strains had ST1 subtypes. Starting after 24 h of incubation, P. cham. subsp. cyprius (1000 μg/mL) exhibited the most pronounced and consistent anti-Blastocystis cytotoxicity against all three strains, comparable to metronidazole. The Ptilostemon chamaepeuce subsp. cyprius anti-Blastocystis cytotoxicity was evident in parasite quantitative distress, morphological alterations, and significant reductions in cell size. Odontites linkii subsp. cyprius cytotoxicity varied among the three Blastocystis strains. The three Blastocystis strains were resistant to Quercus alnifolia. ConclusionP. cham. subsp. cyprius was a potent and promising new herbal extract against Blastocystis sp. in vitro assays.

Using Mycobacterium smegmatis as a Bioindicator for Zinc-limited Growth Conditions in Mycobacteria.

Many bacteria build alternative ribosomes in Zn2+-limiting growth conditions by replacing Zn2+-binding ribosomal proteins with Zn2+-independent paralogs. Defining a system to study these alternative ribosomes has proven difficult because Zn2+ contamination in the laboratory is common. To address this issue, chelating agents are sometimes added to growth media, but this approach convolutes the biological response to gradual Zn2+limitation and is associated with ribosome hibernation. Here, detailed instructions are outlined for preparing media and seeding cultures for Zn2+-limited growth without adding chelators. Following this method, the model bacterium, Mycobacterium smegmatis, undergoes morphogenesis, which depends on alternative ribosomes. Because morphogenesis is tractable and only occurs in Zn2+-limiting conditions, M. smegmatis can be used as a bioindicator to verify biologically relevant growth conditions. Three bioindicator phenotypes (cell density, cell length, and coenzyme F420 fluorescence) that indicate Zn2+ limitation in the wild-type are described, and changes in these bioindicators for a deletion mutant that cannot build alternative ribosomes are outlined. Since trace Zn2+ contamination is difficult to control for each batch of media, and precise quantification of Zn2+ in each media preparation is overly burdensome, following this bioindicator phenotype is an accessible way to validate the preparation of Zn2+-limited growth media. To help identify proper conditions for Zn2+-limiting growth and alternative ribosome production, changes in the bioindicator phenotypes were profiled for Zn2+-contaminated or severely Zn2+-depleted preparations of Zn2+-limited media as well. Further details to achieve Zn2+-limiting growth and alternative ribosome production in M. tuberculosis are presented, along with the associated bioindicator phenotype. Overall, the detailed instructions and bioindicator phenotypes described here will help standardize the production of translationally active alternative ribosomes in mycobacteria.

Lubricant Strategies in Osteoarthritis Treatment: Transitioning from Natural Lubricants to Drug Delivery Particles with Lubricant Properties.

Osteoarthritis (OA) is a debilitating joint disease characterized by cartilage degradation, leading to pain and functional impairment. A key contributor to OA progression is the decline in cartilage lubrication. In physiological conditions, synovial fluid (SF) macromolecules like hyaluronic acid (HA), phospholipids, and lubricin play a crucial role in the boundary lubrication of articular cartilage. In early OA, cartilage damage triggers inflammation, altering SF composition and compromising the lubrication layer. This increases friction between mating interfaces, worsening cartilage degradation and local inflammation. Therefore, early-stage restoration of lubrication (by injecting in the joint different classes of compounds and formulations) could alleviate, and potentially reverse, OA progression. In the light of this, a broad variety of lubricants have been investigated for their ability to reduce friction in OA joints and promote cartilage repair in clinical and preclinical studies. This review examines recent advancements in lubricant-based therapy for OA, focusing on natural, bioinspired, and alternative products. Starting from the currently applied therapy, mainly based on natural lubricants as HA, we will present their modified versions, either in hydrogel form or with specific biomimetic moieties with the aim of reducing their clearance from the joint and of enhancing their lubricating properties. Finally, the most advanced and recent formulation, represented by alternative strategies, will be proposed. Particular emphasis will be placed on those ones involving new types of hydrogels, microparticles, nanoparticles, and liposomes, which are currently under investigation in preclinical studies. The potential application of particles and liposomes could foster the transition from natural lubricants to Drug Delivery Systems (DDSs) with lubricant features; transition which could provide more complete OA treatments, by simultaneously providing lubrication replacement and sustained release of different payloads and active agents directly at the joint level. Within each category, we will examine relevant preclinical studies, highlighting challenges and future prospects.

Public Perceptions of the Food and Drug Administration's Regulatory Authority Over Synthetic Nicotine on Twitter: Observational Study.

The Omnibus Budget Bill, known as H. R. 2471, passed through Congress on March 10, 2022, and was eventually signed by President Biden on March 15, 2022. This bill amended the Federal Food, Drug, and Cosmetic Act granting the Food and Drug Administration (FDA) regulatory authority over synthetic nicotine. This study aims to examine the public perceptions of the Omnibus Bill that regulates synthetic nicotine products as tobacco products on Twitter (rebranded as X). Through the X streaming application programming interface, we collected and identified 964 tweets related to the Omnibus Bill on synthetic nicotine between March 8, 2022, and April 13, 2022. The longitudinal trend was used to examine the discussions related to the bill over time. An inductive method was used for the content analysis of related tweets. By hand-coding 200 randomly selected tweets by 2 human coders respectively with high interrater reliability, the codebook was developed for relevance, major topics, and attitude to the bill, which was used to single-code the rest of the tweets. Between March 8, 2022, and April 13, 2022, we identified 964 tweets related to the Omnibus Bill regulating synthetic nicotine. Our longitudinal trend analysis showed a spike in the number of tweets related to the bill during the immediate period following the bill's introduction, with roughly half of the tweets identified being posted between March 8 and 11, 2022. A majority of the tweets (497/964, 51.56%) had a negative sentiment toward the bill, while a much smaller percentage of tweets (164/964, 17.01%) had a positive sentiment toward the bill. Around 31.43% (303/964) of all tweets were categorized as objective news or questions about the bill. The most popular topic for opposing the bill was users believing that this bill would lead users back to smoking (145/497, 29.18%), followed by negative implications for small vape businesses (122/497, 24.55%) and government or FDA mistrust (94/497, 18.91%). The most popular topic for supporting the bill was that this bill would take a dangerous tobacco product targeted at teens off the market (94/164, 57.32%). We observed a more negative sentiment toward the bill on X, largely due to users believing it would lead users back to smoking and negatively impact small vape businesses. This study provides insight into public perceptions and discussions of this bill on X and adds valuable information for future regulations on alternative nicotine products.