Alternative Feed Ingredients Research Articles

PO384 HDL-c Blood Levels and Frequency of Fish Consumption in a Young Population of Campinas, SP –Brazil

The aquaculture sector is expanding rapidly and needs an increasing supply of fishery products. To ensure an ecological transition of this sector, alternative feed ingredients are required for fish nutrition. Potential alternatives include insects, particularly the black soldier fly (BSF, Hermetia illucens L. 1758), which is being increasingly targeted for their nutritional qualities and their sustainable production practices. BSF have a well-balanced amino acid profile; however, their fatty acid profile is not sufficiently balanced for most aquafeed formulations but can be modulated through their feed. In this study, two different batches of BSF prepupae (BSFP) were firstly produced: BSFP with a standard ω3 content (C18:3n-3 ≈ 1.36%) and ω3-enriched BSFP (C18:3n-3 ≈ 9.67%). Then, three isoproteic, -lipidic and -energetic trout feeds were formulated and produced: one control and two feeds containing 75% BSF meal as a substitute for fish meal (standard vs ω3-enriched-BSF). Finally, a trout feeding trial (n = 3 for each feed batch) in a recirculating aquaculture system was carried out for 75 days. BSFP meal inclusion in trout diets did not impact most nutritional and growth parameters of trout compared to the control; however, the coefficient of fatness increased, weight gain decreased and fatty acid profiles of fillets were altered. In conclusion, this study presents a more sustainable model of trout production by including insects from bioconversion of local byproducts in aquafeed.

Wheat grains fermented by fungal mycelia (Pleurotus ostreatus or Lentinus edodes) as alternative feed ingredients for juvenile rainbow trout (Oncorhynchus mykiss)

Fil: Pascual, Mariano Martin. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Patagonia Norte. Instituto de Investigaciones en Biodiversidad y Medioambiente. Universidad Nacional del Comahue. Centro Regional Universidad Bariloche. Instituto de Investigaciones en Biodiversidad y Medioambiente; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biologia Experimental. Laboratorio de Ecotoxicologia Acuatica; Argentina

Reduction of persistent and semi-persistent organic pollutants in fillets of farmed European seabass (Dicentrarchus labrax) fed low fish oil diets

Traditionally, a major part of aquaculture technology requires fish oil (FO) and fish meal (FM) to produce the aquafeed for farmed species. FO is the main source of persistent organic pollutants (POPs) in fish feed. In recent years, the use of vegetable-origin ingredients in fish feeds has been increasingly studied as an alternative to reduce the levels of these lipophilic pollutants in farmed species. The aim of this study was to evaluate the effect of the use of dietary vegetable oils in the farming of European sea bass (Dicentrarchus labrax) on the contents in persistent - polychlorinated biphenyls (PCBs) and organochlorine pesticides (OCPs) - and semi persistent pollutants - polycyclic aromatic hydrocarbons (PAHs) and polybrominated diphenyl ethers (BDEs) - of their edible parts. A total of 60 seabass muscle pools were obtained from fish farmed employing six experimental diets, which contained different percentages of FO (6 vs. 3%) and FM (20%, 10% and 5%). We did not observe differences in the contamination level of seabass muscle in relation to the percentage of FM in their diet. However, the fish farmed using feed which had lower levels of FO (3%) showed significantly lower muscle levels of ΣPCBs and carcinogenic PAHs (Σc-PAHs), with a reduction of 25.6% and 95.11% (respectively), as compared with those fished raised with feed with higher levels of FO (6%). Also much lower levels were found in OCPs such as sum of DDTs (30.88% of reduction), sum of chlordanes (42.85% of reduction), and sum of BDEs (48.16% of reduction) in those seabass fed with a lower percentage of FO. The results of this study indicate that the use of alternative feed ingredients that allow the employment of low percentage of FO in feeds help to reduce the load of several toxic pollutants in the fillets of European seabass.

영양소 함량을 고려한 육계 대체 원료사료의 대체가격 계산

The objective of this study was to calculate the unit price of an alternative feed ingredient for broiler chickens in consideration of the energy and nutritive contents in the feed ingredient by using a simple Excel worksheet. A corn-distiller’s dried grains with solubles (corn-DDGS) was used as an alternative ingredient and corn and soybean meal as ingredients to be replaced. The net change of feed price was calculated based on the replacement values which were estimated in consideration of energy and nutrient concentration in feed ingredients used in the calculation, the price of feed ingredients and inclusion rate of the alternative ingredient. The nitrogen corrected apparent metabolizable energy(AMEn) and standardized ileal digestible AA including Lys, Met, Thr, and Trp, total Ca, and available P were employed as nutritive component to calculate the replacement values for individual feed ingredients. The equation for replacement was 1 × corn–DDGS + 0.0334 × soybean oil + 0.0182 × Limestone = 0.8893 × corn + 0.13 × soybean meal + 0.0004 × Lys + 0.0022 × Met + 0.0005 × Trp + 0.0028 × Thr + 0.0264 × dicalcium phosphate. The replacement price of corn–DDGS was calculated to be 270 won/kg when the inclusion rate was 15% and the energy and nutrient contents were considered in the calculation. In conclusion, the Excel-based ingredient price calculator may be useful to determine the economic value when an alternative feed ingredient is used in diets fed to broiler chickens.

Improving quality and digestibility of cocoa pod with white rot fungi

White rot fungi is a type of fungus that is able to degrade lignin in the feed material from waste, so it can be used to increase the added value of cocoa pod as alternative feed ingredients to meet the nutritional needs of cattle. The purpose of this study is to investigate the use of white rot fungi in improving the quality and digestibility cocoa pod as feed. The study consisted of two phases, namely fermentation using three isolates of white rot fungi (Coprinus comatus, Corilopsis polyzona and Lentinus torulosus) on pod husks and quality testing in vitro digestibility of fermented. Results of analysis of variance show that the treatment was highly significant on the content of lignin, cellulose and hemicellulose pod husks. Fermented cocoa husks with white rot fungi can degrade lignin content of 1.42% - 12.28% and highly significant improved on in vitro digestibility of dry matter and organic matter. The conclusion, isolates of white rot fungi most active in degrading lignin was Lentinus torulosus isolates and less ability to degrade cellulose and hemicellulose.

PEMANFAATAN LIMBAH KAKAO SEBAGAI PAKAN TERNAK KAMBING

Feed is the main component in livestock industry, and contributes 70% of the total farming cost. Agriculture by-product is one of the alternative feed ingredients for goat feed. The width of cocoa estates in Indonesia in period 2003-2007 shows the highest improvements (43,5 %) than other commodity estates, thus cocoa estates have integrated prospect with livestock industry. Cocoa husk is the most waste of cocoa (73,77 %) and contains raw protein and energy which is not too different with kinggrass, so can substitute green feed more than half. The potential of livestock integrated with the cocoa estates ha a good prospect on the public estate’s development. Cocoa estate has a good prospect to support the integrated with goat farming, however it need s a real concept of the integrated program that work sustainable. The potency of cocoa estate has carrying capacity of 6.05 head goats for 1 hectare area. This is based on the need of the feed from cocoahusk of 1.5 kg/head/day.Keywords : cocoa waste, goat feed

Insect-based protein: future promising protein source for fish cultured

As one of the vital component feed used in fisheries, fishmeal (FM) is generally added to the fish diet to enhance fish growth, digestive performance and absorption of nutrients. This addition contributes significantly to the variable production cost in the aquaculture industry. Expanded production of carnivorous species requiring high protein, high-energy feeds will further tax global fish meal. Thus, research based on the low-cost budget for feed operating cost should be strategized to assist aquaculturists in enhancing fish productivity. Moreover, suitable alternative feed ingredients will have to be utilized to provide the essential nutrients and energy needed to fuel the growth of aquaculture production. To this effect, the use of insect-based protein sources to replace FM that often scarce, expensive, limited availability, and leads to high fish production costs is alternative ways and has been gaining momentum. Currently, Insects have been proposed as one of the potential future protein sources of protein because of the production of insects is highly sustainable. Farming insects is characterized by higher food conversion efficiencies, lower environmental impact, and higher potential to be grown on waste streams.

RETENSI NITROGEN DAN ENERGI METABOLIS RANSUM BROILER YANG MENGANDUNG TEPUNG SILASE KULIT PISANG KEPOK (Musa paradisiaca formatypica).

NITROGEN RETENTION AND METABOLIC ENERGY BROILER RATIONS CONTAINING SILAGE KEPOK BANANA PEEL MEAL. Banana peel is a potential waste used as feed because their numbers are abundant and have the nutrients needed livestock. This study aims to determine the value of nitrogen retention (NR) and nitrogen corrected metabolizable energy value (AMEN) broiler ration containing silage kepok flour banana peel. This study involved 20 birds of broiler strains of Cobb at 6 weeks old. The completely randomized design was applied using four treatments and five replications for each treatment. The treatments were assigned into ration of 55 % corn without peel silage of kepok banana (R0), ration of 46.75% corn with 8.25% peel silage of kepok banana (R1), ration of 38.5% corn with 16.5% peel silage of kepok banana (R2), ration of 30.25% corn with 24.75% peel silage of kepok banana (R3). Variables measured were including nitrogen retention (NR) and nitrogen adjusted metabolic energy (AMEn). Results of analysis of variance showed that treatment significantly different (P <0.05) on nitrogen retention value and highly significant (P <0.01) against the value AMEn. Based on this study it can be concluded that the banana peel silage kepok flour (Musa paradisiaca formatypica) can be used as one of alternative feed ingredients in the ration of broiler replace up to 30% corn 16.5% in the ration seen from Value Retention N (69.68%) while viewed from the AMEn (2924.92 kcal/kg) reimbursement to 15% or 8.25% in the ration. Key word: Kepok banana peel silage, N retention, metabolizable energy, broilers.

Know your fish: A novel compound-specific isotope approach for tracing wild and farmed salmon

The rapid expansion of the aquaculture industry with carnivorous fish such as salmon has been accompanied by an equally rapid development in alternative feed ingredients. This has outpaced the ability of prevailing authentication method to trace the diet and origins of salmon products at the retail end. To close this gap, we developed a new profiling tool based on amino acid δ13C fingerprints. With this tool, we discriminated with high-accuracy among wild-caught, organically, and conventionally farmed salmon groups, as well as salmon fed alternative diets such as insects and macroalgae. Substitution of fishmeal with macroalgae was detected at 5% difference level. The δ13C fingerprints of essential amino acids appear particularly well suited for tracing protein sources, and the non-essentials for tracing lipid origins (terrestrial vs. aquatic). In an industry constantly developing new feed proteins and functional additives, our method is a promising tool for tracing salmon and other seafood products.

Performance, health and physiological responses of newly weaned feedlot cattle supplemented with feed-grade antibiotics or alternative feed ingredients

With increased regulations regarding the use of feed-grade antimicrobials in livestock systems, alternative strategies to enhance growth and immunity of feedlot cattle are warranted. Hence, this experiment compared performance, health and physiological responses of cattle supplemented with feed-grade antibiotics or alternative feed ingredients during the initial 60 days in the feedlot. Angus×Hereford calves (63 steers+42 heifers) originating from two cow–calf ranches were weaned on day −3, obtained from an auction yard on day −2 and road-transported (800 km; 12 h) to the feedlot. Upon arrival on day −1, shrunk BW was recorded. On day 0, calves were ranked by sex, source and shrunk BW, and allocated to one of 21 pens. Pens were assigned to receive (7 pens/treatment) a free-choice total mixed ration containing: (1) lasalocid (360 mg/calf daily of Bovatec; Zoetis, Florham Park, NJ, USA)+chlortetracycline (350 mg/calf of Aureomycin at cycles of 5-day inclusion and 2-day removal from diet; Zoetis) from days 0 to 32, and monensin only (360 mg/calf daily of Rumensin; Elanco Animal Health, Greenfield, IN, USA) from days 33 to 60 (PC), (2) sodium saccharin-based sweetener (Sucram at 0.04 g/kg of diet dry matter; Pancosma SA; Geneva, Switzerland)+plant extracts containing eugenol, cinnamaldehyde and capsicum (800 mg/calf daily of XTRACT Ruminants 7065; Pancosma SA) from days 0 to 32 and XTRACT only (800 mg/calf daily) from days 33 to 60 (EG) or (3) no supplemental ingredients (CON; days 0 to 60). Calves were assessed for bovine respiratory disease (BRD) signs and dry matter intake was recorded from each pen daily. Calves were vaccinated against BRD pathogens on days 0 and 22. Shrunk BW was recorded on day 61, and blood samples collected on days 0, 6, 11, 22, 33, 43 and 60. Calf ADG was greater (P=0.04) in PC v. EG and tended (P=0.09) to be greater in PC v. CON. Feed efficiency also tended (P=0.09) to be greater in PC v. CON, although main treatment effect for this response was not significant (P=0.23). Mean serum titers against bovine respiratory syncytial virus were greater in EG v. PC (P=0.04) and CON (tendency; P=0.08). Collectively, the inclusion of alternative feed ingredients prevented the decrease in feed efficiency when chlortetracycline and ionophores were not added to the initial feedlot diet, and improved antibody response to vaccination against the bovine respiratory syncytial virus in newly weaned cattle.

Effects of cashew nut testa levels as an alternative to wheat bran in gestating sow diets.

ObjectiveThis study was conducted to evaluate the effects of dietary cashew nut testa (CNT) as an alternative feed ingredient to wheat bran on reproductive performance, litter performance, milk composition, and blood profiles of gestating sows.MethodsForth multiparous sows (Yorkshire×Landrace) were fed experimental diets starting at 35 days of pregnancy and an initial average body weight (BW) of 211.53±8.86 kg. Each sow was assigned to a treatment based on BW, backfat thickness (BF) and parity with 10 sows per treatment. Treatments were as follows: i) corn-soybean meal based diet with 6% of wheat bran (C0); ii) basal diet with 2% of CNT and 4% of wheat bran (C2); iii) basal diet with 4% of CNT and 2% of wheat bran (C4); and iv) basal diet with 6% of CNT (C6).ResultsThere were no statistically significant differences in BW and BF of gestating sows throughout the experimental period. However, changes in BF (p = 0.09) and the daily feed intake of sows (p = 0.09) tended to linearly increase during the lactation period. The weaning to estrus interval (WEI) showed a quadratic response to CNT treatment (p = 0.02), and the C2 diet showed the shortest WEI. Litter birth weight (p = 0.04) and piglet birth weight (p = 0.06) were linearly decreased with increase in CNT. Furthermore, there had no significant differences in piglet weight and litter weight in 21 day. Insulin concentration at day 70 of gestation was linearly reduced with increasing CNT level in diets (p = 0.03).ConclusionWhen 6% CNT replaced wheat bran in gestating sow diets, there were no negative effects on sow performance, but litter birth weight and piglet birth weight were decreased when CNT level increased in gestating sow diets.

Beneficial microbial signals from alternative feed ingredients: a way to improve sustainability of broiler production?

SummaryMore sustainable broiler meat production can be facilitated by the increased use of cheap by‐products and local crops as feed ingredients, while not affecting animal performance and intestinal health, or even improving intestinal health, so that antibiotic usage is further reduced. To achieve this, knowledge of the relationship between the taxonomic and functional microbiota composition and intestinal health is required. In addition, the relationship between the novel feed sources, the substrates present in these feed sources, and the breakdown by enzymes and microbial networks can be crucial, because this can form the basis for development of tailored feed‐type specific solutions for optimal digestion and animal performance.

Alternative fish feed production from waste chicken feathers

<p class="Abstract">In this This devotion has been done to provide education and training of the utilization of waste chicken manure, making flour chicken feathers as a fish feed alternative, that can overcome some of the problems that waste chicken feathers from the center cutting broiler chickens in the village Krasak enough, it causes pollution, and not used optimally; Low public awareness of awareness of environmental pollution; the lack of public knowledge about the utilization of waste chicken feathers, and processing technology, as well as to address the needs of fish feed more expensive, need alternative feed ingredients. This service program has provided insight to the public about waste chicken feathers so that it can be used as a new entrepreneurial startups. To achieve these objectives have been done of activity as follows: 1) Provide counseling and understanding of the community will be a negative impact on the environment of waste chicken feathers. 2) Provide counseling utilization of waste chicken feathers for people in nearby farms. 3) Make a chicken feather meal of chicken feather waste as an alternative fish feed to improve digestibility of chicken feathers. 3) The formation of the group for increasing the economic income of the family. This service activities program runs quite well with demonstrated some activity, namely: 1) Change Behavior Society (knowledge transfer); 2) Chicken Feather Extension Waste Utilization; 3) Making Unit Waste Chicken Feathers; 4) Establishment of New Business of Diversified Waste Chicken Feathers.</p>

A survey of the mycobiota associated with larvae of the black soldier fly (Hermetia illucens) reared for feed production.

Feed security, feed quality and issues surrounding the safety of raw materials are always of interest to all livestock farmers, feed manufacturers and competent authorities. These concerns are even more important when alternative feed ingredients, new product developments and innovative feeding trends, like insect-meals, are considered. The black soldier fly (Hermetia illucens) is considered a good candidate to be used as feed ingredient for aquaculture and other farm animals, mainly as an alternative protein source. Data on transfer of contaminants from different substrates to the insects, as well as the possible occurrence of toxin-producing fungi in the gut of non-processed insects are very limited. Accordingly, we investigated the impact of the substrate/diet on the intestinal mycobiota of H. illucens larvae using culture-dependent approaches (microbiological analyses, molecular identification through the typing of isolates and the sequencing of the 26S rRNA D1/D2 domain) and amplicon-based next-generation sequencing (454 pyrosequencing). We fed five groups of H. illucens larvae at the third growing stage on two substrates: chicken feed and/or vegetable waste, provided at different timings. The obtained results indicated that Pichia was the most abundant genus associated with the larvae fed on vegetable waste, whereas Trichosporon, Rhodotorula and Geotrichum were the most abundant genera in the larvae fed on chicken feed only. Differences in the fungal communities were highlighted, suggesting that the type of substrate selects diverse yeast and mold genera, in particular vegetable waste is associated with a greater diversity of fungal species compared to chicken feed only. A further confirmation of the significant influence of diet on the mycobiota is the fact that no operational taxonomic unit common to all groups of larvae was detected. Finally, the killer phenotype of isolated yeasts was tested, showing the inhibitory activity of just one species against sensitive strains, out of the 11 tested species.

Former food products safety: microbiological quality and computer vision evaluation of packaging remnants contamination

ABSTRACTThe use of alternative feed ingredients in farm animal’s diets can be an interesting choice from several standpoints, including safety. In this respect, this study investigated the safety features of selected former food products (FFPs) intended for animal nutrition produced in the framework of the IZS PLV 06/14 RC project by an FFP processing plant. Six FFP samples, both mash and pelleted, were analysed for the enumeration of total viable count (TVC) (ISO 4833), Enterobacteriaceae (ISO 21528-1), Escherichia coli (ISO 16649-1), coagulase-positive Staphylococci (CPS) (ISO 6888), presumptive Bacillus cereus and its spores (ISO 7932), sulphite-reducing Clostridia (ISO 7937), yeasts and moulds (ISO 21527-1), and the presence in 25 g of Salmonella spp. (ISO 6579). On the same samples, the presence of undesired ingredients, which can be identified as remnants of packaging materials, was evaluated by two different methods: stereomicroscopy according to published methods; and stereomicroscopy coupled with a computer vision system (IRIS Visual Analyzer VA400). All FFPs analysed were safe from a microbiological point of view. TVC was limited and Salmonella was always absent. When remnants of packaging materials were considered, the contamination level was below 0.08% (w/w). Of note, packaging remnants were found mainly from the 1-mm sieve mesh fractions. Finally, the innovative computer vision system demonstrated the possibility of rapid detection for the presence of packaging remnants in FFPs when combined with a stereomicroscope. In conclusion, the FFPs analysed in the present study can be considered safe, even though some improvements in FFP processing in the feeding plant can be useful in further reducing their microbial loads and impurity.

Physicochemical Characteristics, in Vitro Fermentation Indicators, Gas Production Kinetics, and Degradability of Solid Herbal Waste as Alternative Feed Source for Ruminants

The aims of this research were to study the nutrient and secondary metabolite contents of solid herbal wastes (SHW) that were preserved by freeze drying, sun drying and silage, as well as to analyze their effects on in vitro fermentation indicators i.e., gas production kinetics and degradability of solid herbal waste. Physical and chemical properties on three forms of SHW (sun dry, freeze dry, and silage) were characterized and then an in vitro gas production experiment was performed to determine the kinetics of gas production, methane production, NH3, microbial protein, and SHW degradability. Polyethylene glycol (PEG) was added to the three treatments to determine the biological activity of tannins. Results showed that all three preparations of SHW still contained high nutrient and plant secondary metabolite contents. Gas production, methane, NH3, microbial protein, in vitro degradability of dry matter (IVDMD) and organic matter (IVDOM) of SHW silage were lower (P<0.05) compared to sun dry and freeze dry. These results were apparently due to the high content of secondary metabolites especially tannin. It can be concluded that solid herbal wastes (SHW) can be used as an alternative feed ingredients for ruminants with attention to the content of secondary metabolites that can affect the process of fermentation and digestibility in the rumen.

PENAMPILAN PRODUKSI TERNAK BABI GROWER YANG MENGGUNAKAN TEPUNG KULIT KOPI SEBAGAI BAHAN PENGGANTI SEBAGIAN DEDAK HALUS PADA PAKAN

GROWER PRODUCTION PERFORMANCE USE FLOUR COFFEE PULP AS SUBSTITUTION MATERIALS TO A PARTS OF FINE BRAN ON FEED. Various feed ingredients or waste that have not been utilized for example the skin of coffee fruit can be an alternative feed ingredient source of crude fiber see its content in the skin of the fruit coffee is 33.60%. North Sulawesi, especially Bolaang Mogondow, which is a center of coffee plantation, has a plantation area of 4,097.7 ha, with coffee production reaching 2,135.46 tons of raw material which has not been separated between coffee fruit with its leaves and stems. After the separated coffee fruit obtained as much as 1,288.22 tons per year. Coffee fruit is processed to separate between the seeds and skin after separated obtained by the skin of coffee fruit as much as 448,449 tons. The appearance of production by looking at weight gain, the amount of consumption and the efficiency of feed use is part of the requirement to see the production capability of pigs. The study was conducted for 66 days consisting of 52 days of data collection period and 2 weeks preliminary collecting data aims to determine the effect of coffee skin use as a substitute of some fine bran on the production performance of the grower pigs to the finisher. The study used 16 male and female pigs grower period aged 12-16 weeks descent kinds Yorkshire and Landrace. Randomized block design with four levels of treatment that is 0, 25%, 50%, 75% coffee pulp as a partial replacement of fine bran in 4 groups. Differences in body weight is a replication of the study. The result of analysis of variance (ANOVA) showed that the powder of coffee husk as substitute of bran to the weight gain gave real effect (P &lt;0.05), the amount of feed consumption did not give real effect (P&gt; 0.05) Feed gave a real effect. The result is the use of coffee leaf powder as a substitute of some fine bran in grower pigs until finisher can provide good growth and efficient use of good feed.Keywords: Phase grower, finisher, flour coffee skin

A Potential of Azolla sp. inclusion in Catfish (Clarias sp.) Feed Formulation Againts Energy Retention and Feed Conversion Ratio.

Catfish is one of the fish that has a high economical value. Catfish is accepted by society easily because of its advantages. These advantages include fast growing, good adaptive behavior, good taste, high nutrient, and cheap price. One of the factors that determine the success of aquaculture is the availability of adequate food, both quantity and quality. There are several alternative feed ingredients that can be used in the preparation of feed. One of these feed ingredients is azolla flour made from Azolla sp. plant. Azolla sp. plant contains large amount of protein up to 28,12% of its dried weight. Selection of the base material feed using Azolla sp. based on the research results Handajani (2000) which declare that the protein content Azolla sp. which is quite high, namely 28.12% dry weight. The addition of Azolla sp. in catfish feed formulation is expected to improve retention of energy and lower feed conversion ratio. The purpose of this study was to determine the addition of Azolla sp. in feed formulations affect the retention of energy and feed conversion ratio catfish. This research was conducted in July-August in the Faculty of Fisheries and Marine University Airlangga. Research method used in the study was an experimental method with complete random planning. It included five treatments and four repetitions. Treatments that were used included azolla flour inclusion 0%, 5%, 10%, 15% and 20% at the formulated feed. Data analysis that was used included statistic and descriptive. The result of the research shows that energy retention among treatments does not have significant difference. On the other hand, the feed conversion ratio also does not have significant difference. It can be concluded that Azolla sp. inclusion in the catfish feed formulation affects nothing to energy retention and feed conversion ratio. Keyword : Azolla sp., Clarias sp., Energy Retentiom, Feed Convertion Ratio

Subtitution Leaf Fermentation Leucaena (Leucaena leucocephala) on Feed vaname shrimp (Litopenaeus vannamei) for Retention of Protein and Energy

Intensive aquaculture systems causing heavy reliance on the use of artificial feed (Heptarina et al., 2010). Feed is a crucial component to the success of shrimp farming (Soemardjati and Suriawan, 2006). According to Haryanto and Djajanegara (1993) lamtoro leaves contain protein, calcium and energy. The content of the leaves lamtoro a source of protein for feed ingredients in this plant are essential amino acids lysine and leucine were very high (D'Mello and Fraser, 1981). The consideration for the selection of feed materials should be done in accordance with the provisions of the feed material that is readily available, low cost, high nutrient content and do not compete with humans (Handajani et al., 2010). According to Matthew (1993), leucaena as forage quality has not been used optimally and not much commercialized. Lamtoro a potential biological resources to be used as feed to the waste generated sufficient forage nutritional value (Widiastuti, 2007). Research on the use of fermented flour substitution lamtoro leaf is a matter that needs to be done because of the use of fermented flour lamtoro leaf is expected to improve the quality of feed and make it as an alternative feed ingredient that can be given to increase aquaculture production. This research is the experimental method using a completely randomized design (CRD) with four treatments and five replications. The results of this study will be analyzed descriptively in the form of data and tables. Data obtained, processed using Analysis of Variance (ANOVA) to determine the effect of different treatments applied if real, then continued with Duncan's Multiple Range Test (Duncan's Multiple Range Test) with a 5% error rate to determine the best treatment. Keywords: Feed, Vaname Shrimp, Fermentation, Substitution, Leucaena Leaf, Protein Retention, Retention Energy.

Nutritional analysis of alternative feed ingredients and their effects on the larval growth of Tenebrio molitor (Coleoptera: Tenebrionidae)

AbstractAlternative feeds comprising only brewer's spent grain (BSG) or distillers dried grain (DDG), both agricultural byproducts, and 50% BSG or 50% DDG mixed with 50% wheat bran (WB) were compared to the standard 100% WB feed provided to Tenebrio molitor larvae to evaluate their effects on the nutritional quality and economic viability of mass‐producing this insect. Crude protein content was highest in larvae fed on 100% WB and 100% DDG, and the latter had 2.32 and 1.88 times more essential and non‐essential amino acids than the 100% WB group, respectively. Larvae fed on 50% DDG + 50% WB had 1.30 times more saturated fatty acids but 1.08 times less unsaturated fatty acids and 1.03–1.23 times less potassium, phosphorus, and magnesium than larvae fed on 100% WB. Traces of mercury were detected in larvae fed on 100% WB and 50% DDG + 50% WB, although at levels below the General Food and Feed Standard (Ministry of Food and Drug Safety, Republic of Korea), but neither Escherichia coli or Salmonella spp. were detected in the feeds. Larval and pupal weight, larval development and survival, and pupation rate were higher in larvae fed on 50% BSG or 50% DDG mixed with 50% WB groups than in larvae fed on 100% WB. Therefore, using 50% BSG or 50% DDG mixed with 50% WB as an alternative feed in the mass production of T. molitor provides higher quality larvae at a lower cost than does using the conventional 100% WB feed.