Alteration Products Research Articles

FRI288 Single-Cell Transcriptomics Identifies Pituitary Gland Cell Type Population Changes In Diet Induced Obesity

Abstract Disclosure: R.E. Ruggiero-Ruff: None. B. Le: None. N. Lainez: None. D. Coss: None. Obesity is a worldwide growing public health concern, with a current prevalence of 43% in the United States and projected future increase. Obesity is associated with type 2 diabetes, cardiovascular disease, stroke, and endocrine abnormalities. The pituitary gland is the master gland that responds to metabolic perturbations and regulates homeostasis via hormone secretion. Thyrotrophic, gonadotropic, somatotropic, lactotropic and corticotropic dysfunctions may occur as a consequence of obesity and perpetuate pathophysiologic conditions since pituitary hormones regulate metabolic function in return. Previous work from our lab determined that mice fed high fat diet have reduced levels of gonadotropin hormones, indicating that diet-induced obesity may play a role in pituitary hormone production. We postulate that diet-induced obesity alters the pituitary transcriptome and/or select population changes. To determine the effect of diet-induced obesity on pituitary gland population plasticity, we performed single-cell RNA-sequencing (scRNA-seq) of pituitary glands from control and high-fat diet-fed mice and characterized population changes and gene expression changes in each population. While gonadotrope population changes levels of gene expression that results in the lower gonadotropin levels, somatotrope and lactotrope populations, on the other hand, change at the population level with alterations in the cell numbers that result in altered levels of growth hormone and prolactin in the circulation. In summary, we defined diet-induced changes in pituitary hormone production and population shifts that may play a role in altered hormone production in obese patients and consequently dysregulation of homeostasis. Presentation: Friday, June 16, 2023

Plasma Aβ level alterations after sleep deprivation correspond to brain structural remodeling in medical night shift workers

The relationship between brain structure alteration and metabolic product clearance after night shift work with total sleep deprivation (SD) remains unclear. Twenty-two intensive care unit staff on regularly rotating shift work were implemented with structural and diffusion MRI under both rest wakefulness (RW) and SD conditions. Peripheral blood samples were collected for the measurement of cerebral metabolites. Voxel-based morphometry and diffusion tensor imaging analysis were used to investigate the alterations in the gray matter density (GMD) and mean diffusivity (MD) within the participants. Furthermore, correlation analysis was performed to investigate the relationship between the neuroimaging metrics and hematological parameters. A significant increase in the GMD values was observed in the anterior and peripheral areas of the brain under SD. In contrast, a decrease in the values was observed in the posterior regions, such as the bilateral cerebellum and thalamus. In addition, a significant reduction in the total cerebrospinal fluid volume was observed under SD. The Aβ42/Aβ40 levels in participants under SD were significantly lower than those under RW. The mean MD increment values extracted from the region of interest (ROI) of the anterior brain were negatively correlated with the increment of plasma Aβ42/Aβ40 levels (r = −0.658, P = 0.008). The mean GMD decrement values extracted from the posterior ROI were positively correlated with the increment of plasma Aβ-40 levels (r = 0.601, P = 0.023). The findings of this study suggest that one night of shift work under SD induces extensive and direction-specific structural alterations of the brain, which are associated with aberrant brain metabolic waste clearance.

Characterization of the Angat Ophiolite: New insights from bulk major and trace element geochemistry and petrographic analysis

The Angat Ophiolitic Complex, located north-northeast of Manila, Luzon is perhaps one of Luzon’s most fundamental suspect terranes. The definite age of the ophiolite is not well-established, with various authors having different claims. Encarnacion et al. (1993) conducted a U-Pb age dating for the Angat wherein the Angat Ophiolite was found to have an Early Middle Eocene age (48 Ma), close to the ZOC age - leading to the assumption that the two ophiolites are related and underlie most of Central Luzon. Hence, this paper aims to collect samples from the Eocene and Cretaceous Angat Ophiolite for petrographic and geochemical analyses, identify the relationship between the separated basalt units from the basalts in the main ophiolite body, and compare the geochemistry of the Eocene and Cretaceous units of the Angat Ophiolite with the other well-known ophiolites from the literature. Field investigations were conducted in five important localities wherein 15 samples were obtained. The results highlighted that the major mineral phases in the samples collected are plagioclase and augite, with some samples having hornblende and olivine. All samples appear to have undergone low-grade greenschist facies metamorphism, which may be attributed to hydrothermal alteration. Chloritization of pyroxene minerals is also evident, along with the hydrothermal alteration products of plagioclase and pyroxenes. The relationship between the EAO, MOC, and separated basalt patch in Marilaque Highway is identified. The Coto Block of the Zambales Ophiolite and the Angat Ophiolite may be related and formed over a mantle initially enriched by a subduction component.

Human umbilical cord mesenchymal stem cells ameliorate colon inflammation via modulation of gut microbiota-SCFAs-immune axis

BackgroundInflammatory bowel disease (IBD) is a global health problem in which gut microbiota dysbiosis plays a pivotal pathogenic role. Mesenchymal stem cells (MSCs) therapy has emerged as a prospective novel tool for managing IBD, and which can also regulate the composition of gut microbiota. However, the functional significance of MSCs-induced changes in gut microbiome is poorly understood.MethodsHere, we investigated for the first time the role of gut microbiota in mediating the protective effect of human umbilical cord MSCs (HUMSCs) on DSS-induced colitis. Gut microbiota alteration and short-chain fatty acids (SCFAs) production were analyzed through 16S rRNA sequencing and targeted metabolomics. Spectrum antibiotic cocktail (ABX), fecal microbiota transplantation (FMT) and sterile fecal filtrate (SFF) were employed to evaluate the protective effect of intestinal flora and its metabolites. Cytokine microarray, Enzyme-linked immunosorbent assay (ELISA), and flow cytometry were conducted to assess the effect on CD4+T homeostasis.ResultsHere, we investigated for the first time the role of gut microbiota in mediating the protective effect of MSCs on DSS-induced colitis. By performing gut microbiota depletion and fecal microbiota transplantation (FMT) experiments, we revealed that MSCs derived from human umbilical cord ameliorated colon inflammation and reshaped T-cells immune homeostasis via remodeling the composition and diversity of gut flora, especially up-regulated SCFAs-producing bacterial abundance, such as Akkermansia, Faecalibaculum, and Clostridia_UCG_014. Consistently, targeted metabolomics manifested the increased SCFAs production with MSCs administration, and there was also a significant positive correlation between differential bacteria and SCFAs. Meanwhile, combined with sterile fecal filtrate (SFF) gavage experiments, the underlying protective mechanism was further associated with the improved Treg/Th2/Th17 balance in intestinal mucosa mediated via the increased microbiota-derived SCFAs production.ConclusionThe present study advances understanding of MSCs in the protective effects on colitis, providing evidence for the new role of the microbiome-metabolite-immune axis in the recovery of colitis by MSCs.

How super is supersulfide?: Reconsidering persulfide reactivity in cellular biology

In an attempt to understand the cellular mechanisms of H2S signalling, recent research has focused on supersulfide (i.e., alkyl and inorganic hydropersulfide) formation and subsequent reactivity. While our understanding of supersulfides in biology has rapidly advanced, there are some chemical features of this unique functional group that require re-evaluation. Persulfides, such as glutathione hydropersulfide, have been called “supersulfide” as it is assumed that the alkyl hydropersulfide (RSSH) functional group is a superior nucleophile compared to the corresponding thiol (RSH) due to the alpha effect. However, recent quantum mechanical calculations and experimental data show that persulfides are not “super” nucleophiles, but rather potent electrophiles in cellular biology. It is proposed here that persulfides, via their electrophilic signalling effects, induces a cellular hormesis effect, which may explain the observed effects of altered RSSH production. Therefore, the electrophilic and thiol oxidant properties of persulfides should considered in cellular biology.

Tracing titanomagnetite alteration with magnetic measurements at cryogenic temperatures

SUMMARY Titanomagnetite containing up to 0.6–0.7 Ti atoms per formula unit is a primary magnetic mineral phase in submarine basalts and in some terrestrial volcanic rocks. On a geological timescale, it often undergoes alteration, forming new magnetic phases that may acquire (thermo)chemical remanent magnetization. The initial stage of this natural process can be modelled by prolonged laboratory annealing at moderately elevated temperatures. In this study, our goal is to characterize the alteration products resulting from annealing a submarine basalt containing homogeneous titanomagnetite Fe3−xTixO4 (x ≈ 0.46) at temperatures of 355, 500 and 550 °C for up to 375 hr, by examining their magnetic properties over a wide range of temperatures. The effect of extended annealing is most apparent in the low-temperature magnetic properties. In the fresh sample, a magnetic transition is observed at 58 K. Below the transition temperature, the field-cooled (FC) and zero-field-cooled (ZFC) saturation isothermal remanent magnetization (SIRM) curves are separated by a tell-tale triangular-shaped area, characteristic for titanomagnetites of intermediate composition. The room-temperature SIRM (RT-SIRM) cycle to 1.8 K in zero field has a characteristic concave-up shape and is nearly reversible. For the annealed samples, the magnetic transition temperature shifts to lower temperatures, and the shape of the curves above the transition changes from concave-up to concave-down. The shape of the RT-SIRM cycles also progressively changes with increasing annealing time. The SIRM loss after the cycle increases up to ∼30 per cent for the samples annealed for 375 hr at 355 °C, and for 110 hr at 500 and 550 °C. The Curie temperatures of the newly formed magnetic phases exceed the Curie temperature of the fresh sample (205 °C) by up to 350 °C. While this effect is most commonly attributed to extensive single-phase oxidation (maghemitization), the behaviour observed at cryogenic temperatures appears incompatible with the known properties of highly oxidized titanomaghemites. Therefore, we propose that, at least in the initial stage of the ‘dry’, that is, not involving hydrothermalism, alteration of titanomagnetite, temperature- and time-controlled cation reordering is the primary mechanism driving changes in both low- and high-temperature magnetic properties.

Crk and Crkl Are Required in the Endocardial Lineage for Heart Valve Development.

Background Endocardial cells are a major progenitor population that gives rise to heart valves through endocardial cushion formation by endocardial to mesenchymal transformation and the subsequent endocardial cushion remodeling. Genetic variants that affect these developmental processes can lead to congenital heart valve defects. Crk and Crkl are ubiquitously expressed genes encoding cytoplasmic adaptors essential for cell signaling. This study aims to explore the specific role of Crk and Crkl in the endocardial lineage during heart valve development. Methods and Results We deleted Crk and Crkl specifically in the endocardial lineage. The resultant heart valve morphology was evaluated by histological analysis, and the underlying cellular and molecular mechanisms were investigated by immunostaining and quantitative reverse transcription polymerase chain reaction. We found that the targeted deletion of Crk and Crkl impeded the remodeling of endocardial cushions at the atrioventricular canal into the atrioventricular valves. We showed that apoptosis was temporally increased in the remodeling atrioventricular endocardial cushions, and this developmentally upregulated apoptosis was repressed by deletion of Crk and Crkl. Loss of Crk and Crkl also resulted in altered extracellular matrix production and organization in the remodeling atrioventricular endocardial cushions. These morphogenic defects were associated with altered expression of genes in BMP (bone morphogenetic protein), connective tissue growth factor, and WNT signaling pathways, and reduced extracellular signal-regulated kinase signaling activities. Conclusions Our findings support that Crk and Crkl have shared functions in the endocardial lineage that critically regulate atrioventricular valve development; together, they likely coordinate the morphogenic signals involved in the remodeling of the atrioventricular endocardial cushions.

The Mineral and Geochemical Features of Sulfides in the Jade Hydrothermal Field of the Okinawa Trough in Off-Shore China

In this study, mineralogical and elemental geochemical characteristics of massive sulfide samples collected from the Jade hydrothermal field, located in the Izena depression in the central graben of the Okinawa Trough, were analyzed by means of optical microscopy, scanning electron microscopy, and electron probe microanalysis (EPMA). The results show that the mineralization in the Jade hydrothermal field can be divided into Zn-Cu-Pb-rich massive sulfides and Zn-Fe-rich massive sulfides. The former is composed of sphalerite, galena, chalcopyrite, pyrite, and anglesite, which is the product of the low-temperature alteration of galena. The latter is mainly composed of sphalerite, pyrite, marcasite, and traces of galena. Cu and Zn in pyrite may exist in the form of microinclusions, while Ag and Pb may exist in pyrite in the form of fine galena inclusions containing Ag. Fe and Cu may enter sphalerite in the form of ion replacement. Zn may enter chalcopyrite in the form of ion replacement. Consistent with the previous understanding, the metal elements in the hydrothermal liquid system in the Jade hydrothermal field mostly migrated as sulfur complexes, and when the hydrothermal fluid mixes with seawater, the physical and chemical conditions of the fluid change, resulting in sulfide mineral precipitation. However, the chemical structure of chalcopyrite is still controversial, which restricts the understanding of the substitution mechanism of trace elements during chalcopyrite precipitation.

Association between hyperketolactia and production in early-lactating dairy cows

Study aims were to investigate associations of hyperketolactia (HYKL) status of Holstein dairy cows between 6 and 60 d in milk (DIM), defined by milk acetone (mACE) and β-hydroxybutyrate (mBHB) content, with daily milk yield and composition. Milk samples (∼5.0 million) were collected over a 5-yr period (2014-2019) within the milk recording system in Poland. Concentrations of mACE and mBHB determined by Fourier-transform infrared spectroscopy were used to categorize samples into 4 ketolactia groups. Based on threshold values of ≥0.15 mmol/L mACE and ≥0.10 mmol/L mBHB, ketolactia groups were normoketolactia (NKL; mACE <0.15 mmol/L and mBHB <0.10 mmol/L), BHB hyperketolactia (HYKLBHB; mACE <0.15 mmol/L and mBHB ≥0.10 mmol/L), ACE hyperketolactia (HYKLACE; mACE ≥0.15 mmol/L and mBHB <0.10 mmol/L), and ACE and BHB hyperketolactia (HYKLACEBHB; mACE ≥0.15 mmol/L and mBHB ≥0.10 mmol/L). To investigate ketolactia association with production outcomes, a linear model was developed, including ketolactia group, DIM, parity, their interactions, year-season as fixed effects, and random effects of herd and cow. Among all milk samples, 31.2% were classified as HYKL, and of these, 52.6%, 39.6%, and 7.8% were HYKLACEBHB, HYKLBHB, and HYKLACE, respectively. Ketolactia groups differed for all traits studied in all parities and DIM. Among HYKL groups, lowest milk yield was found in HYKLACEBHB cows, except for 6 to 30 DIM in first- and second-lactation cows. Milk yield of HYKLBHB cows was higher than that of NKL cows until 20 to 30 DIM, and then it was lower than NKL cows. Milk yield of HYKLACE cows was mostly lower than NKL cows. Energy-corrected milk (ECM) yield of HYKLACEBHB cows was higher than that of NKL cows until 30 to 35 DIM for second lactation and third lactation or greater, and in the whole study period for first lactation. The yield of ECM for HYKLBHB cows was mostly higher than that of NKL cows, whereas HYKLACE cows had higher ECM than NKL cows until 15 to 25 DIM and then was lower for the HYKLACE group. Milk composition differed among HYKL groups. Highest milk fat (MF) and lowest milk lactose (ML) contents were observed in HYKLACEBHB cows. Cows in HYKLACEBHB and HYKLBHB groups had higher MF and lower milk protein (MP; except in 6-8 DIM in first lactation) and ML content than NKL cows. Milk fat content was higher in HYKLACE than NKL cows in first lactation and during the first 30 to 40 DIM in older cows. Lactose content was lower in HYKLACE than in NKL cows within 30 to 40 DIM; afterward it was higher in NKL cows. Lower MP content was found in HYKLACE than in NKL cows, except during 6 to 9 DIM for cows in first lactation and third lactation or greater. In conclusion, HYKL is associated with altered milk production in all parities, but a range of these negative relations depends on ketone status addressing both ACE and BHB contents. Further research is needed to ascertain underpinning biochemical defects of HYKL from elevated ACE, alone or in combination with BHB, during early lactation.

Uranium pre-concentration in sandstone-hosted U deposits: A case study from the Hailijin ore field, SW Songliao Basin, NE China

Economic U mineralization in sandstone was commonly attributed to oxygenated fluid infiltration in reduced sandstone, leading to U precipitation in redox front during late diagenetic stage. It was proposed that U can be pre-concentrated before this period based on trace amounts of U in altered products of Fe-Ti oxides, as revealed by electron microprobe analysis (EMPA). However, the obtained trace elements concentrations in altered Fe-Ti oxides (<1 wt%) is generally accompanied with relatively low analytical precision, leading to a skeptical explanation under this situation. In this work, laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) was used to determine trace elements concentrations in hematite from the uraniferous Lower Yaojia Formation (Hailijin ore field, SW Songliao Basin, NE China), aiming to fingerprint this proposed U pre-concentration in details. Some trace elements contents, such as Mo, Ge, Ca, P, Y, Pb, Th, Sn, and W, are positively correlated with U content in hematite. Given that uraniferous oxygenated groundwater with a near-neutral pH is unable to efficiently dissolve detrital phosphate (i.e., apatite and monazite) in the Lower Yaojia Formation, phosphorous must have been released from these P-rich phases before late diagenesis. Such a conclusion is supported by our observations that monazite from the reduced sandstone has a corrosive morphology, and exhibits a corresponding U enrichment in the corrosive rim. The dissolved P and U can be adsorbed by porous titania up to several thousand ppm during early diagenesis, providing a pre-concentrated metal source for remobilization during late diagenesis. It can not only account for a trace-element signature derived from monazite (i.e., Ca, P, Y, Pb, and Th) in hematite geochemistry, but also fingerprint Mo and Ge migrations resulted from oxidation of pyrite and organic matter, respectively. In addition to U, tungsten and Sn would be re-equilibrated with infiltrated oxidized fluid during the recrystallization of porous titania, and partially incorporated into simultaneously formed hematite grains due to high compatibility. The remobilization of pre-concentrated U induced by oxygenated fluid incursion led to U accumulation in a redox front during late diagenesis, forming uraninite nanoaggregates with relatively high concentrations of P, Y and Ti. Our work has verified that trace-element signature in hematite from sandstone is a reliable fingerprint to reveal ore-forming processes, and we propose for the first time that titania and phosphate play a crucial role for U pre-concentration in sandstone-hosted U deposits.

Small-Scale Food Production in the Pandemic: Perspectives from Mexico and Guatemala / Producción de alimentos en pequeña escala durante la pandemia: perspectivas desde México y Guatemala

In lieu of an abstract, here is a brief excerpt of the content: As researchers conducting fieldwork in the era of COVID-19 in Mexico and Guatemala, we observed how the pandemic dramatically altered small-scale food production systems. Given the differentiated impacts of COVID-19 on agri-food systems and the diversity of strategies implemented across scales in Mexico and Central America (Lopez-Ridaura et al., 2021), we present three perspectives to highlight the variability in socioeconomic disruptions, local market alterations, and responses among (a) smallholder members of Guatemalan farmer organizations involved in agroecology from A.M. Rice, (b) agroecological producers in Oaxaca, Mexico from L.M. Choi, and (c) small-scale fishers in Baja California Sur (BCS), Mexico from J.W. Dean. We find variability in the effects of supply chain disruptions and the spectrum of shifts to alternative products or markets, as mediated through localized producer networks. We focus on the immediate impacts and responses to the pandemic (reporting primarily on fieldwork between 2020–2022).

Associations of postprandial ghrelin, liver-expressed antimicrobial peptide 2 and leptin levels with body composition, disease progression and survival in patients with amyotrophic lateral sclerosis.

Loss of appetite contributes to weight loss and faster disease progression in amyotrophic lateral sclerosis (ALS). Impairment of appetite control in ALS may include altered production or action of orexigenic (i.e., ghrelin) and anorexigenic (i.e., liver-expressed antimicrobial peptide 2 [LEAP2] and leptin) hormones. We aimed to determine if postprandial circulating ghrelin levels, LEAP2 levels, LEAP2:ghrelin molar ratio and leptin levels differ in ALS patients compared to non-neurodegenerative disease controls, and whether they are associated with disease progression and body composition. In this prospective natural history study, we assessed postprandial plasma levels of ghrelin, LEAP2 and leptin in patients with ALS (cases; n = 46) and controls (controls; n = 43). For cases, measures were compared to changes in body weight, body composition and clinical outcomes. Postprandial ghrelin level was decreased by 52% in cases compared to controls (p = 0.013). LEAP2:ghrelin molar ratio was increased by 249% (p = 0.009), suggesting greater ghrelin resistance. Patients with lower LEAP2:ghrelin tended to have better functional capacity at assessment, as inferred by the ALS Functional Rating Scale-Revised (τ = -0.179, p = 0.086). Furthermore, ghrelin and LEAP2:ghrelin molar ratio correlated with diagnostic delay (ghrelin, τ = 0.223, p = 0.029; LEAP2:ghrelin, τ = -0.213, p = 0.037). Baseline ghrelin level, LEAP2 level, LEAP2:ghrelin ratio and leptin level were, however, not predictive of change in functional capacity during follow-up. Also, patients with higher postprandial ghrelin levels (hazard ratio [HR] 1.375, p = 0.048), and lower LEAP2:ghelin ratios (HR 0.828, p = 0.051) had an increased risk of earlier death. Reduced postprandial ghrelin levels, coupled with increased LEAP2:ghrelin molar ratios, suggests a loss of ghrelin action in patients with ALS. Given ghrelin's actions on appetite, metabolism and neuroprotection, reduced ghrelin and greater ghrelin resistance could contribute to impaired capacity to tolerate the physiological impact of disease. Comprehensive studies are needed to explain how ghrelin and LEAP2 contribute to body weight regulation and disease progression in ALS.

Multiscale evidence for weathering and the preservation of carbonaceous material in an Antarctic micrometeorite

Micrometeorites (MMs) recovered from the Earth’s surface may have undergone significant changes prior to their collection, including weathering while residing in the terrestrial environment. These alteration processes, such as the precipitation of hydrous phases, may overprint both primary and atmospheric entry features, obscuring pre-existing material properties. In addition, weathering exerts a prominent control on the geochemical interactions, such as species mobility, between extraterrestrial material and the terrestrial environment, particularly in Antarctica. In this study, we have characterised the textural and compositional consequences of weathering on an unmelted, fine-grained, Antarctic MM, which includes the mapping of nanometre-scale features using atom probe tomography. In particular, we investigate geochemical behaviour across textural boundaries in the MM and observe nanoscale elemental heterogeneity within complex alteration assemblages. In one sample region, a compositional boundary is highlighted by distinct elemental differences, consistent with a weathering encrustation of mixed mineralogies, while analyses in other targeted regions show evidence for nanoscale elemental networks, as well as a grain boundary adjacent to a carbon-rich region. We discuss our findings in the context of terrestrial weathering as a dominant cause for the nanoscale features observed. Weathering processes responsible for these features include the leaching of extraterrestrial material, precipitation of secondary alteration products with associated layering, and the influence of mechanical stress on pre-existing weaknesses. From these results, we derive a weathering sequence to explain the formation of the alteration product assemblage and highlight the controls on MM geochemistry in the terrestrial environment. Our observations show that nanoscale carbonaceous material may be preserved in oxy-hydroxides under icy conditions, which can also act as tracers for local environmental changes.

Identification of carbonate-associated sulfate (CAS) in a Noachian Martian meteorite Allan Hills 84001

The aqueous environment and possible habitability of early Mars have been widely studied based on orbital and in situ explorations, as well as analyses of Martian meteorites. Using microscale X-ray absorption near edge structure (μ-XANES) analysis, we report the first sulfur (S) speciation of the carbonates in a Martian meteorite, Allan Hills 84001, precipitated in the 4-billion-year-old aqueous alteration on Mars. The XANES data show diagnostic signatures of oxidized sulfur in the carbonates, indicating that carbonate-associated sulfate (CAS) formed from coexisting sulfate ions (SO42−) in the aqueous fluid. A thermodynamic calculation suggests that the CAS deposited from a fluid with a moderately oxidizing to reducing and neutral to slightly alkaline pH condition. The possible sources of SO42− ions are the minor SOx species in the Noachian atmosphere and/or the supply from volcanic gas. It is concluded that considerable amounts of the atmospheric volatiles including CO2 and SOx may have been stored as alteration products (e.g., carbonates) in the Martian underground system.

Pre- and syn-impact formation of clay minerals at the Ries impact structure, Germany: Implications for clay minerals on Mars

The presence of extensive clay minerals in the ancient Noachian terrains of Mars is often used to invoke past climatic conditions that were warmer and supported surface-stable liquid water. These clay-rich regions are also heavily cratered, leading to the possibility of a causal relationship. The aim of this study is to better understand the impact excavation and generation of clays and whether there are any mineralogical or geochemical indicators that could differentiate between these two origins, both on Earth and, by analogy, Mars. Here, we present a detailed field and laboratory investigation of the composition, texture, and setting of clay minerals in impactites at the well-preserved Ries impact structure, Germany. Authigenic impactite (syn- and post-impact) clay minerals in impact melt-bearing breccia deposits are compared with sedimentary-derived clay mineral-bearing units preserved from the time of the impact event. Our findings indicate: (1) impact-generated deposits comprise compositionally diverse, Al-dominant smectitic clay minerals that could have formed without appreciable exogenous volatiles through a combination of autometamorphism, hydrothermal alteration, and devitrification; and (2) the pre-impact sedimentary clay mineral assemblages were similar in composition to those in the impact-generated deposits such that only detailed, successive laboratory treatments and analyses could discern the two sample types. NASA’s Perseverance Mars rover mission is presently investigating its first science campaign and has identified secondary alteration products, including possible clay minerals. Our study suggests that the rover may explore impact-generated clay minerals in situ, though their provenance might only be determined from analysis of the returned samples in Earth laboratories.

Effect of Diet and Oxidative Stress in the Pathogenesis of Lymphoproliferative Disorders.

Lymphomas are a heterogeneous group of pathologies that result from clonal proliferation of lymphocytes. They are classified into Hodgkin lymphoma and non-Hodgkin lymphoma; the latter develops as a result of B, T, or NK cells undergoing malignant transformation. It is believed that diet can modulate cellular redox state and that oxidative stress is implicated in lymphomagenesis by acting on several biological mechanisms; in fact, oxidative stress can generate a state of chronic inflammation through the activation of various transcription factors, thereby increasing the production of proinflammatory cytokines and causing overstimulation of B lymphocytes in the production of antibodies and possible alterations in cellular DNA. The purpose of our work is to investigate the results of in vitro and in vivo studies on the possible interaction between lymphomas, oxidative stress, and diet. A variety of dietary regimens and substances introduced with the diet that may have antioxidant and antiproliferative effects were assessed. The possibility of using nutraceuticals as novel anticancer agents is discussed; although the use of natural substances in lymphoma therapy is an interesting field of study, further studies are needed to define the efficacy of different nutraceuticals before introducing them into clinical practice.

The Beacon Heights “Tinguaite” (Phonolite): A recrystallised agpaitic ignimbrite in the Mesoproterozoic Pilanesberg alkaline complex, South Africa

AbstractAn extrusive rock unit in the Mesoproterozoic Pilanesberg alkaline complex that has been referred to as the “Beacon Heights Tinguaite” since the work of Shand (1928) has been reexamined in the field and studied by petrographic microscopy, scanning electron microscopy, and whole-rock major and trace element analysis. The new observations indicate that the unit (here informally renamed the “Beacon Heights Phonolite” to comply with current petrographical nomenclature) is a laminated, volcanic rock in which mafic layers rich in sodic pyroxene alternate on any scale with discontinous, felsic layers consisting of alkali feldspar, nepheline, sodalite and their alteration products natrolite and analcime. Highly strontian apatite, fluorite, a manganiferous pectolite-group mineral, sphalerite and pyrrhotite are minor to accessory minerals. In addition, the rock carries indicator minerals typical of agpaitic rocks (including lamprophyllite and eudialyte-group minerals and their replacement products). The rock contains abundant crystal fragments of felsic minerals (alkali feldspar, nepheline and sodalite), fragments and elongated lenses of nepheline syenite, some of which contain primary magmatic lamprophyllite, and rare armoured lapilli. The overall structure of the rock is that of a welded ashflow tuff (igmimbrite) affected by post-magmatic recrystallisation processes. Whole-rock major and trace element compositions suggest a compositional affinity with members of the agpaitic, intrusive Green Foyaite Suite of the Pilanesberg Complex, and probably also a genetic relationship. The Beacon Heights Phonolite is a rare, possibly unique, example of an ashflow deposit of agpaitic, highly silica-undersaturated composition.

Manganese-Iron Phosphate Nodules at the Groken Site, Gale Crater, Mars

The MSL Curiosity rover investigated dark, Mn-P-enriched nodules in shallow lacustrine/fluvial sediments at the Groken site in Glen Torridon, Gale Crater, Mars. Applying all relevant information from the rover, the nodules are interpreted as pseudomorphs after original crystals of vivianite, (Fe2+,Mn2+)3(PO4)2·8H2O, that cemented the sediment soon after deposition. The nodules appear to have flat faces and linear boundaries and stand above the surrounding siltstone. ChemCam LIBS (laser-induced breakdown spectrometry) shows that the nodules have MnO abundances approximately twenty times those of the surrounding siltstone matrix, contain little CaO, and have SiO2 and Al2O3 abundances similar to those of the siltstone. A deconvolution of APXS analyses of nodule-bearing targets, interpreted here as representing the nodules’ non-silicate components, shows high concentrations of MnO, P2O5, and FeO and a molar ratio P/Mn = 2. Visible to near-infrared reflectance of the nodules (by ChemCam passive and Mastcam multispectral) is dark and relatively flat, consistent with a mixture of host siltstone, hematite, and a dark spectrally bland material (like pyrolusite, MnO2). A drill sample at the site is shown to contain minimal nodule material, implying that analyses by the CheMin and SAM instruments do not constrain the nodules’ mineralogy or composition. The fact that the nodules contain P and Mn in a small molar integer ratio, P/Mn = 2, suggests that the nodules contained a stoichiometric Mn-phosphate mineral, in which Fe did (i.e., could) not substitute for Mn. The most likely such minerals are laueite and strunzite, Mn2+Fe3+2(PO4)2(OH)2·8H2O and –6H2O, respectively, which occur on Earth as alteration products of other Mn-bearing phosphates including vivianite. Vivianite is a common primary and diagenetic precipitate from low-oxygen, P-enriched waters. Calculated phase equilibria show Mn-bearing vivianite could be replaced by laueite or strunzite and then by hematite plus pyrolusite as the system became more oxidizing and acidic. These data suggest that the nodules originated as vivianite, forming as euhedral crystals in the sediment, enclosing sediment grains as they grew. After formation, the nodules were oxidized—first to laueite/strunzite yielding the diagnostic P/Mn ratio, and then to hematite plus an undefined Mn oxy-hydroxide (like pyrolusite). The limited occurrence of these Mn-Fe-P nodules, both in space and time (i.e., stratigraphic position), suggests a local control on their origin. By terrestrial analogies, it is possible that the nodules precipitated near a spring or seep of Mn-rich water, generated during alteration of olivine in the underlying sediments.

Exploring geothermal resources using electromagnetic methods in coastal areas of volcanic islands: Challenges of nearshore and land 3D controlled-source electromagnetic data

Three-dimensional geophysical exploration is essential in identifying favorable areas and derisking high-temperature geothermal projects. In particular, electrical resistivity imaging plays a key role in this exploration due to its sensitivity to the presence of alteration products, geothermal fluid circulation, and temperature. As 3D seismic exploration is rarely effective in volcanic environments, resistivity methods such as magnetotellurics (MTs) are often used to extract deep structural information. However, deep electromagnetic (EM) imaging in coastal areas of volcanic islands with MT can be challenging due to anthropogenic noise induced by urbanized areas concentrated around the coast. The use of active EM sources, such as airborne EM (AEM) and controlled-source EM (CSEM), as a complement to MT is a solution for overcoming anthropogenic noise. However, applying these methods in this context is still challenging due to the proximity to the sea/land interface, large variations in topography and nearshore bathymetry, and the heterogeneity of the near surface. Our approach outlines the challenges of acquiring, processing, and inverting nearshore and land 3D CSEM data in such complex environments. The CSEM data are part of a multimethod geothermal exploration on the island of Martinique in the French West Indies, and its 3D inversion result is compared with the previous regional 3D MT and AEM inversion results. In addition to MT, CSEM may highlight the location of a potential high-temperature paleo-reservoir at shallow depths under the urbanized area of Petite-Anse covered by a paleo-clay cap ranging from a few meters to several hundred meters in thickness. We conclude by proposing possible improvements for CSEM and multimethod methodologies to obtain more reliable exploratory models.

Facies and Hydrocarbon Reservoir Rock Characterization of the Paleozoic Rocks of Peshawar Basin, Northwest Pakistan

The present study details the facies description and hydrocarbon reservoir characterization of the Paleozoic rocks of Peshawar Basin, northwest Pakistan. The outcrop samples from the Cambrian-Devonian rocks along the famous Nowshera-Risalpur Road and Turlandi Village Section were investigated. The analysis of outcrop data revealed significant information regarding the facies and their depositional environments. Based on its detailed sedimentological data, it is believed that the Ambar Formation is deposited in shallow shelf-tidal flat settings, while the protolith of the Misri Banda Quartzite came from the wide beaches. The protolith of the Panjpir Formation was deposited in the shelf conditions, which shows an overall shallowing when moving up the section from the argillites and phyllites to crinoidal limestone. The Nowshera Formation was deposited as a reef complex on the shelf edge having reef core, reef breccia and a back-reef lagoon. The techniques of petrography, XRD and SEM were used for the bulk geochemical composition of the rocks focusing on their matrix, mineralogy, micro-porosity and pore-filling materials. The presence of micrite, goethite, kaolinite and illite as intergranular mass, dolomitization-induced porosity, twin cleavage plane and high dissolution porosity in the Ambar Formation can provide significant pore space to the reservoir fluids migration. Hematite with minor kaolinite, illite and chlorite as alteration products of unstable framework grains as intergranular mass and a deep seated burial diagenesis has minimized the reservoir potential in Misri Banda Quartzite. The coarse and fine calcite, dolomite, chlorite and kaolinite occur as pore-filling material between the framework grains while the high intensity of intragranular dissolution and micro intergranular connecting porosity in the Nowshera Formation makes it a promising reservoir.