Alpine Lakes Research Articles

Evaporation and sublimation measurement and modeling of an alpine saline lake influenced by freeze–thaw on the Qinghai–Tibet Plateau

Abstract. Saline lakes on the Qinghai–Tibet Plateau (QTP) affect the regional climate and water cycle through water loss (E, evaporation under ice-free conditions and sublimation under ice-covered conditions). Due to the observational difficulty over lakes, E and its underlying driving forces are seldom studied when targeting saline lakes on the QTP, particularly during ice-covered periods (ICP). In this study, the E of Qinghai Lake (QHL) and its influencing factors during ice-free periods (IFP) and ICP were first quantified based on 6 years of observations. Subsequently, three models were calibrated and compared in simulating E during the IFP and ICP from 2003 to 2017. The annual E sum of QHL is 768.58±28.73 mm, and the E sum during the ICP reaches 175.22±45.98 mm, accounting for 23 % of the annual E sum. E is mainly controlled by the wind speed, vapor pressure difference, and air pressure during the IFP but is driven by the net radiation, the difference between the air and lake surface temperatures, the wind speed, and the ice coverage during the ICP. The mass transfer model simulates lake E well during the IFP, and the model based on energy achieves a good simulation during the ICP. Moreover, wind speed weakening resulted in an 7.56 % decrease in E during the ICP of 2003–2017. Our results highlight the importance of E in ICP, provide new insights into saline lake E in alpine regions, and can be used as a reference to further improve hydrological models of alpine lakes.

Interactive effects of climate-atmospheric cycling on aquatic communities and ecosystem shifts in mountain lakes of southeastern Tibetan Plateau

Recent climate warming and atmospheric reactive nitrogen (Nr) deposition are affecting a broad spectrum of physical, ecological and human systems that may be irreversible on a century time scale and have the potential to cause regime shifts in ecological systems. These changes may alter the limnological conditions with important but still unclear effects on lake ecosystems. We present changes in cladoceran with comparisons to diatom assemblages over the past ~200 years from high-resolution, well-dated sediment cores retrieved from six high mountain lakes in the southeastern (SE) margin of the Tibetan Plateau. Our findings suggest that warming and the exponential increase of atmospheric Nr deposition are the major drivers of ecological regime changes. Shifts in cladoceran and diatom communities in high alpine lakes began over a century ago and intensified since 1950 CE, indicating a regional-scale response to anthropogenic climate warming. Zooplankton in the forest lakes showed asynchronous trajectories, with increased Nr deposition as a significant explanatory factor. Forest lakes with higher dissolved organic carbon (DOC) concentrations partially buffered the impacts of Nr deposition with little structural change, while lakes with low DOC display symptoms of resilience loss related to Nr deposition. Biological community compositional turnover in subalpine lakes has shown marked shifts, equivalent to those of low-elevation lakes strongly affected by direct human impacts. This suggests that local effects override climatic forcing and that lake basin features modified by anthropogenic activity act as basin-specific filters of common forcing. Our results indicate that snow and glacial meltwaters along with nutrient enrichment related to climate warming and atmospheric Nr deposition, represent major threats for lake ecosystems, even in remote areas. We reveal that climate and atmospheric contaminants will further impact ecological conditions and alter aquatic food webs in higher altitude biomes if climate and anthropogenic forcing continue.

Impacts of climate warming and atmospheric deposition on recent shifts in chironomid communities in two alpine lakes, eastern China

Climate warming and atmospheric deposition are altering alpine lake ecosystems at unprecedented rates, whereas their direct and indirect effects on primary consumer communities are unclear. This study presents sedimentary multi-proxy records including chironomids, diatoms, elements and stable isotopes of carbon and nitrogen in 210Pb-dated cores from two alpine lakes located above the timberline in the Taibai Mountain, eastern China. Before ∼2000 CE, chironomid communities were co-dominated by Heterotrissocladius marcidus-type and Micropsectra atrofasciata-type in the two lakes. Thereafter, Tanytarsus glabrescens-type increased rapidly to be a dominant species. Redundancy analyses (RDAs) revealed that chironomid fauna shifts were significantly correlated with rising diatom concentrations in both lakes, declining Ti content in the upstream lake and δ13C depletion in the downstream lake. Although temperature, precipitation and δ15N were not significant explanatory variables in RDAs, climate warming and atmospheric deposition likely promoted terrestrial and aquatic primary production, indicated by synchronous increases in organic matter contents and diatom concentrations in the two sediment cores. Since diatoms contain essential polyunsaturated fatty acids that are essential for chironomids, rising diatom concentrations can promote food quantity and quality. In addition, increased primary production would create organic substrates for chironomid larvae. Recent shifts in chironomid fauna driven by indirect effects of global warming and atmospheric deposition might be a widespread phenomenon in alpine lakes, probably triggering regime shifts in headwater lake ecosystems.

Water temperature, mixing, and ice phenology in the arctic–alpine Lake Darfáljávri (Lake Tarfala), northern Sweden

ABSTRACT In the rapidly warming circumpolar Arctic, recent research of lakes has focused on their climatology and ecology but is challenged by sparsity of wintertime data. At the c. 48-m-deep and c. 0.5-km2 large proglacial Darfáljávri (Lake Tarfala), located in an arctic–alpine environment in the Scandinavian Mountains, year-round water temperatures were previously reported for 2016 to 2019. Here, this record is continued for 2019–2020 and 2021–2022, complemented by time-lapse imagery records of the state of the lake surface, as well as degree-day modeling of ice phenology (timing of ice-on and ice-off). Darfáljávri is cryostratified during winter, with interannual variations in the thermocline’s thickness and temperature range. The ice season lasts from October to July. Modeled ice-on dates match observed ones reasonably well; however, observed ice-off dates occur much later than modeled ones, likely because of cold impact from Darfáljávri’s glacial environment as inferred from a comparison with a close tundra lake. Though new insights into the complex lake mixing and ice phenology are provided, it remains to attribute the characteristics of Darfáljávri’s winter stratification to additional potential drivers, such as lake ice thickness, atmospheric heat fluxes, and the water balance of the lake.

Unknown localities and new species of flora Chechen Republic (North-Eastern Caucasus)

The paper presents data on unknown localities and new species of flora of the Chechen Republic, which we discovered during expeditionary research in 2022-2023 on the territory of the Makazhoi semiarid basin. The study area belongs to arid ecosystems with unique species and coenotic diversity. Nearby there is a unique natural monument and tourist attraction - the alpine lake Kezenoy-Am. In recent years, the tourism industry has been actively developing here, which inevitably leads to anthropogenic transformation of the flora and increases the risk of losing the uniqueness, originality, and characteristic features of the flora and vegetation of mountain landscapes. For the first time, we provide information about the discovery of 11 new species, previously unknown for the republic, belonging to 9 genera and 8 families. In our research, we strive to emphasize the need for in-depth study and conservation of the biodiversity of these ecosystems for future generations.

Reconstruction of temperature and monsoon precipitation in southwestern China since the last deglaciation

Monsoon precipitation and temperature are the crucial components of the Indian monsoon climate, yet their spatial-temporal relationship remains unclear. In this study, we reconstruct the changes in monsoon precipitation and mean annual temperature simultaneously over the past 16,000 years using multiple proxies, including isoprenoid glycerol dialkyl glycerol tetraethers (isoGDGTs) and n-alkanes, from an alpine lake in southwestern China. On the one hand, the reconstructed monsoon precipitation shows a long-term increasing trend during the last deglaciation and a gradual decrease during the Holocene, with the highest-humidity period occurring in the early Holocene. Our result displays good consistency with the changes in regional mean annual precipitation, summer precipitation, and monsoon intensity during the last deglaciation and the Holocene. On the other hand, the reconstructed mean annual temperature inferred from the n-alkane proxy shows a long-term warm trend since the last deglaciation. Our result is consistent with the regional reconstruction of both mean annual and summer temperatures during the last deglaciation, while evident seasonal differences were observed for Holocene records with a cooling trend of summer temperature and a warm trend of mean annual temperature. Comprehensive comparison reveals that monsoon precipitation in the Indian monsoon region is mainly related to changes in the Intertropical Convergence Zone (ITCZ) and land-sea thermal contrast controlled by boreal summer insolation. In addition, the increase in temperature during deglaciation is primarily attributed to the escalation of greenhouse gases (GHGs), while the divergent trend of seasonal temperature during the Holocene is mainly attributed to the changes in local seasonal insolation. Our research emphasizes that monsoon precipitation is a summer signature in the Indian monsoon region, which is consistent with the changes in summer temperature, while contrasting with the changes in mean annual temperature. Therefore, it is necessary to consider the seasonal difference in temperature changes when investigating the hydrothermal combination in Indian monsoon regions.

Lakes-scale pattern of eukaryotic phytoplankton diversity and assembly process shaped by electrical conductivity in central Qinghai-Tibet Plateau.

Phytoplankton are the main primary producers in aquatic ecosystems and play an important role in food web and geochemical cycles. Its diversity, community structure, and assembly process are influenced by several factors. Alpine lake ecosystems are relatively weak and extremely sensitive to global climate change. However, the impact of climate change on phytoplankton in Qinghai-Tibet Plateau lakes and their responses are still unclear. In this study, we analyzed the diversity, environmental drivers, and assembly process of phytoplankton community in the central QTP lakes. The phytoplankton of these lakes can be primarily distinguished into freshwater and brackish types, with significant differences in species diversity and community dissimilarity. Both shared nearly same key environmental factors that significantly affecting phytoplankton such as EC, and brackish lakes were also positively correlative with TN. Stochastic process was predominant in phytoplankton assembly. Additionally, freshwater and brackish lakes were dominated by dispersal limitation and heterogeneous selection respectively. Alpine lakes had significant EC thresholds, and their diversity and assembly processes changed significantly around the thresholds. The present findings have important implications for understanding and predicting the response of lake phytoplankton communities to climate change and for making decisions to protect the ecological resources of alpine lakes.

Trace elements in the water column of high-altitude Pyrenean lakes: Impact of local weathering and long-range atmospheric input

High altitude (alpine) lakes are efficient sentinels of environmental processes, including local pollution and long-range atmospheric transfer, because these lakes are highly vulnerable to ongoing climate changes and increasing anthropogenic pressure. Towards improving the knowledge of trace element geochemistry in the water column of alpine lakes, we assessed 64 physico-chemical parameters, including macro- and micronutrients, major and trace element concentrations in the water column of 18 lakes in the Pyrenees, located along the border between France and Spain.Lake depth, morphology, retention time and watershed rock lithology did not exhibit sizable impact on major and trace element concentrations in the water column. However, acidic (pH = 4.7 ± 0.2) lakes were distinctly different from circumneutral lakes (pH = 6.8 ± 0.5) as they exhibited >10 times higher concentrations of SO42− and trace metals (Fe, Mn, Zn, Cd, Pb, Co, Ni, Be, Al, Ga and REEs). While some of these elements clearly mark the presence of sulphide-rich minerals within the watershed (Fe, Zn, Cd and Pb), the increased mobility of lithogenic elements (Be, Al, Ga and REEs) in acidic lakes may reflect the leaching of these elements from silicate dust derived from atmospheric deposits or surrounding granites. At the same time, compared to circumneutral lakes, acidic lake water displayed lower concentrations of dissolved oxyanions (As, Mo, V, B and W) and elevated SO42− concentrations. The latter could lead to efficient Ba removal from the water column. The exploitation of metal ores within the watershed of three lakes clearly impacted high Zn and Cd concentrations observed in their water column, despite two of these lakes not being acidic. We conclude that local impacts have a greater effect on the water column than long-range atmospheric inputs and that dissolved trace element concentration measurements can be used for revealing sulphide-rich minerals or acid mine drainage within the lakes’ watershed.

Glacier Retreat Leads to the Expansion of Alpine Lake Karakul Observed Via Remote Sensing Water Volume Time Series Reconstruction

Due to high altitudes, Central Asian alpine lakes can serve as indicators of localized climate change. This article monitored the water volume time series trends of the ungauged alpine Lake Karakul, which is typical because of the abundance of glaciers in the basin, from 1990 to 2020 via multiple source remote sensing data. The “Global-Local” multi-scale lake extraction method is used to delineate the boundary of Lake Karakul. Consistency analysis was performed on the altimetry data of CryoSat-2, ICESat-1 and ICESat-2, assuming that the lake surface was flat; a threshold value was set to remove gross error, and then 3σ was used to remove the surface elevation anomaly. Based on the pyramid volume model, the lake area and surface elevation information were used to reconstruct the water volume time series of Lake Karakul. The influencing factors of water volume temporal variation were discussed. The results show that Lake Karakul has been on an expansionary trend in recent years: The lake area increased from 394.9 km2 in 1988 to 411.4 km2 in 2020; the rate of increase is 0.74 m/year. The surface elevation increased from 3886.6 m in 2003 to 3888.6 m in 2020; the rate of increase is 0.11 km2/year. The lake water volume accumulated was 0.817 km3 in 2003–2020, with an accumulation rate of 0.059 km3/year. The Lake Karakul basin is developing towards dry heat, with a cumulative temperature variation rate of +0.38 °C/year; the average rate of variation in annual cumulative precipitation is −3.37 mm/year; the average evapotranspiration in the watershed is on a fluctuating increasing trend, with a rate of variation of +0.43 mm/year; glaciers in the lake basin have a retreating trend, with an average annual rate of variation of −0.22 km2/year from 1992 to 2020. Lake Karakul is more sensitive to temperature variations, and the runoff from retreating glaciers in the basin is an important contribution to the expansion of Lake Karakul.

Wrack line formation and composition on shores of a large Alpine lake: The role of littoral topography and wave exposure.

Wrack lines are a key formation along shorelines that provide organic matter and bring ecological diversity to the local environment. Although wrack line formation has been extensively studied along marine beaches and estuaries, in contrast, knowledge about the environmental variables that promote wrack line formation within inland lakes is widely lacking. In one of the first studies to focus on wrack line formation on lakesides, we analysed the dimensions, volume, elevation and particulate composition of 36 wrack lines across 20 shore sections of a large, oligotrophic Alpine lake with natural water level fluctuations (Lake Constance-Obersee). Using multivariate partial least squares (PLS) regression, we identified the key environmental variables that drive wrack accumulation in lakeside areas. Our results demonstrate that wrack line volume increased with (1) the width of the eulittoral zone as an indicator of the swash conditions (up-rush vs. down-wash), (2) high exposure to wind waves as indicated by the total effective fetch, (3) high exposure to ship waves (catamaran ferry), and (4) the width of the sublittoral zone as an indicator of the availability of source material (Chara spp.) and of the wave energy dissipation rate of the incoming deep water waves. Sediment texture played only a minor role. Wide eulittoral zones and high ship wave exposure favoured high proportions of lake-borne components (Chara remains, mollusc shells), while the reverse was true for land-based components. Anthropogenic wastes were only present in small proportions. We discuss four main factor groups influencing the amount of wrack in marine beaches and on lakeshores considering similarities (waves, breakers, swash, dissipation, relief) and differences (tides vs. annual water level fluctuations) of the two systems, and point out research gaps. We demonstrate that wrack line formation is also important in large inland lakes and can be analysed using basic ideas from relevant marine studies.

Effects of nitrogen on benthic diatom assemblages in high-elevation central and eastern alpine lakes

ABSTRACT We explored patterns of benthic diatom composition across sixty-two high-elevation alpine lakes spanning a wide range of nitrogen (N) concentrations due to atmospheric deposition and background variation in lake and watershed characteristics. Our goals were to (1) assess the effect of lake water N concentration on benthic diatom composition during late summer or fall conditions and (2) identify policy-relevant response thresholds. The analyses were carried out on a large set of diatom and water chemistry data, integrated with new data. Multivariate and correlation analyses revealed associations between pH, N concentration, and benthic diatom composition, but the effects of pH and N were confounded. However, partial correlation analysis allowed us to identify “N-responsive diatoms”; that is, diatom taxa with nonspurious associations with N. Focusing on these taxa, we detected a decline in the abundance of taxa preferring low N concentrations and an increase in the abundance of taxa preferring high N concentrations starting at NO3 concentrations of approximately 5 µmol L−1. We interpreted this shift as an effect of watershed N saturation due to atmospheric deposition. Based on the results, we suggest a late-summer or fall concentration threshold of 5 µmol NO3 L−1 to prevent change in benthic diatoms in high-elevation alpine lakes.

Lake Sevan. Past, present, and future state of a unique alpine lake

Lake Sevan, a large, deep, alpine lake in the Lesser Caucasus is the focus of this Special Issue of the Journal of Limnology. It was an outstanding ecosystem 100 years ago characterised by excellent water quality, rich biodiversity with a high level of endemism, wide-ranging beds of macrophytes along the shores and a productive and sustainable fish production. Due to its beauty, natural history, and contributions to social and economic welfare it is also a cultural heritage for the Armenian Nation including its large diaspora.

Contemporary community composition, spatial distribution patterns, and biodiversity characteristics of zooplankton in large alpine Lake Sevan, Armenia

We studied the quantitative composition, spatial distribution, and temporal dynamics of the zooplankton community of the alpine Lake Sevan, Armenia, the largest surface water in the Caucasus region. This article is providing a long-term information and fills the research gap of multiyear data on zooplankton, as the previous research on zooplankton provided only snapshots of the community, and a consistent assessment over multiple years was missing. However, an initial mini-review of historical studies indicated that zooplankton biomass and fish abundance were undergoing large fluctuations, indicating the importance of top-down control. We analysed 239 samples from the period 2016-2019 from 32 sampling sites in Lake Sevan and recorded 37 species of meso- and macrozooplankton (Rotifers, Copepods, Cladocera). Biomass fluctuations were high with peaking biomasses in 2016 and lowest biomasses in 2018, yearly averaged biomass varied about one order of magnitude. Variability over time was hence much higher than spatial variability. The pelagic habitat at the deepest part of the lake showed the highest diversity and biomasses but contrasts between sampling sites remained smaller than changes from year to year or seasonally. Many samples were dominated by a single species, and these key species explain observed biomass dynamics to a wide extent. We applied hierarchical clustering in order to identify phenological groups that appear to show similar patterns of occurrence. This clustering resulted in 6 groups where of 5 groups just consisting of one single species and these 5 key species were the Cladocerans Daphnia magna, Daphnia hyalina, Diaphanosoma sp. as well as the calanoids Arctodiaptomus bacillifer and Acanthodiaptomus denticornis. The most important species in Lake Sevan’s zooplankton during the observation period was D. magna, which reached high biomasses in 2016 and 2017 but then suddenly almost disappeared in 2018 and 2019. When there were more D. magna present, the water became clearer, which was measured using Secchi depth. This shows that these large water fleas effectively controlled the amount of phytoplankton in the water. Daphnia magna, in turn, managed to dominate zooplankton community only during times of extremely low fish biomass indicating strong top-down control of this large Cladoceran by fish. Both observations together imply a strong trophic linkage between fish, zooplankton, and phytoplankton and provide evidence for trophic cascades in Lake Sevan. Besides the novel insights into zooplankton community dynamics of this unique lake of high socio-economical, cultural, and ecological importance, our study also points to potential management opportunities for eutrophication control by biomanipulation, as well as our investigation allows us to conclude that probably biotic factors were more important than abiotic factors in explaining the observed changes and dynamics within the plankton community.

The prevalence of Atribacteria affiliated with JS1 in the sediment core of Lake Sayram, the largest alpine lake, China

Atribacteria play an important role in global carbon cycling. Little is known, however, about the Atribacteria communities found inalpine lakes. The presence and diversity of Atribacteria in the sediment core of alpine Sayram lake in China were investigated usingIllumina MiSeq sequencing in this study. According to the findings, Atribacteria affiliated with JS1 were dominant in the sediment coreof Sayram Lake, with a relative abundance of 0.19 to 10% (average 3.75%) of all bacterial sequences. Furthermore, the relativeabundance of Atribacteria associated with JS1 increased with sediment depth. As a result, this study significantly contributes to ourunderstanding of Atribacteria community habitat.

Two new Achnanthidium (Bacillariophyceae) species from a recent alpine lake in the National Park of Ecrins (South of France)

Two new Achnanthidium (Bacillariophyceae) species from a recent alpine lake in the National Park of Ecrins (South of France)

Phosphorus-driven regime shift from heterotrophic to autotrophic diazotrophs in a deep alpine lake

Biological nitrogen fixation plays a critical role in maintaining primary production, particularly in systematic nitrogen deficiency. However, little is known about the dynamics within diazotrophic community facing ongoing nutrient enrichment in freshwater lakes. Here, a consecutive five-year investigation on diazotrophic community was conducted in Lake Fuxian, an oligotrophic deep alpine lake on the trajectory to eutrophic state. Results showed a regime shift from heterotrophic to autotrophic diazotrophs induced by total phosphorus (TP) enrichment. Specifically, heterotrophic diazotrophs dominated the diazotrophic community when TP was lower than 21.8 μg/L, whereas heterotrophic diazotrophs or diazotrophic Cyanobacteria randomly dominated when TP ranged between 21.8 μg/L and 28.8 μg/L. When TP was higher than 28.8 μg/L, diazotrophic Cyanobacteria accounted for 60.4%-97.7% of the total N2-fixers, indicating diazotrophic biodiversity significantly declined under TP enrichment scenario. Moreover, the dominance of diazotrophic Cyanobacteria further facilitated phytoplankton growth, which strengthened positive feedback between phytoplankton and phosphorus under nitrogen deficiency conditions. This is the first report on the threshold-like state responses of freshwater diazotrophs to environmental drivers. Our study expands the knowledge of the diazotrophic dynamics in freshwater ecosystems and contributes quantitative evidence of ecological thresholds for future environmental policymaking.

Trace Level Analysis of Per- and Polyfluorinated Substances in Fish from Various Regions in Switzerland.

Per- and polyfluorinated substances (PFASs) are persistent man-made chemicals which can end up in the food chain. In this study, the concentrations of 15 PFASs in various wild fish species from different regions in Switzerland were determined excluding hot spots of contamination. After clean-up with SPE, the samples were analyzed by HPLC-MS/MS. PFASs were detected in all but 1 of the 83 fish samples (0.07 to 40.7 µg/kg fish muscle meat). The most abundant compound in fish from subalpine lakes was perfluorooctane sulfonic acid (PFOS), comprising more than 80% of the total contamination while perfluorononanoic (PFNA), -decanoic (PFDA) and -undecanoic (PFUnDA) acid dominated in high alpine fish. PFAS levels were more elevated in subalpine lakes (median PFASs 11.1-19.0 µg/kg) than in the high alpine Lake Sils (median PFASs 0.66-2.67 µg/kg) or streams and canals in Valais (median PFASs 0.56 µg/kg). Our results indicate that wild fish may be one of the PFAS sources in human diet.

Sediment Bacteria in the Alpine Lake Sayram: Vertical Patterns in Community Composition.

Bacterial communities inhabiting alpine lakes are essential to our understanding of ecosystem processes in a changing climate, but little has been reported about the vertical patterns of sediment bacterial communities in alpine lakes. To address this knowledge gap, we collected the 100 cm long sediment core from the center of Lake Sayram, the largest alpine lake in Xinjiang Uygur autonomous area, China, and used 16S rRNA gene-targeted amplicon sequencing to examine the bacterial populations. The results showed that bacterial diversity, as estimated by the Shannon index, was highest at the surface (6.9849 at 0-4 cm) and gradually decreased with depth up to 3.9983 at 68-72 cm, and then increased to 5.0927 at 96-100 cm. A total of 56 different phyla and 1204 distinct genera were observed in the sediment core of Lake Sayram. The bacterial community structure in the sediment samples from the various layers was dissimilar. The most abundant phyla in alpine Lake Sayram were Proteobacteria, Firmicutes, and Planctomycetes, accounting for 73%, 6%, and 4% of the total reads, respectively; the most abundant genera were Acinetobacter, Hydrogenophaga, and Pseudomonas, accounting for 18%, 12%, and 8% of the total reads, respectively. Furthermore, the relative abundance of Acinetobacter increased with sediment depth, while the relative abundance of Hydrogenophaga and Pseudomonas decreased with sediment depth. Our findings indicated that the nitrate-reducing bacteria (Acinetobacter, Hydrogenophaga, and Pseudomonas) may be prevalent in the sediment core of Lake Sayram. Canonical correspondence analysis showed that carbonate and total organic carbon (TOC) may be the main environmental factors affecting the vertical patterns of bacterial community composition (BCC) in the sediment of Lake Sayram. This work significantly contributes to our understanding of the BCC of sediments from alpine lakes in arid and semiarid regions.

Effects of microbial-converted ancient permafrost organic carbon on the growth and reproduction of Daphnia magna.

Immense amounts of ancient (radiocarbon age over 200years) organic carbon (OC) from permafrost are released into aquatic systems. Ancient terrestrial OC exists in numerous aquatic ecosystems. It has been reported that ancient OC can be incorporated by consumers in aquatic ecosystems, but the effect of ancient OC on the growth of consumers has rarely been studied. In this study, we extracted ancient dissolved organic carbon (DOC) from frozen soils in an alpine lake catchment. After a 6-day microbial conversion period, the contents of ω3 and ω6 polyunsaturated fatty acids (PUFAs) in ancient DOC increased. Proteobacteria and Actinobacteria were the primary taxa consuming the permafrost DOC and generating fatty acids. In addition to the exclusive diet of soil DOC (containing bacteria) or Chlorella pyrenoidosa, mixed diets of Chlorella pyrenoidosa, and ancient DOC (containing bacteria) in ratios of 2:1, 1:1, and 1:2 (by carbon concentration) were used to feed Daphnia magna. We discovered that Daphnia reared on the mixture with the DOC:Chlorella ratio of 1:2 had the highest contents of ω3 PUFAs and FAs. Daphnia reared exclusively on Chlorella and the mixture with the DOC:Chlorella ratio of 1:2 had the largest body size (3.1-3.4mm) and the highest offspring production (95.5-96.2 ind-1). Daphnia fed on mixed diets exhibited higher intrinsic rates of population growth (0.48-0.53 d-1) compared to those fed exclusively on Chlorella pyrenoidosa, or ancient DOC plus bacteria. Overall, ancient soil OC converted by bacteria can act as a valuable supplement to algae food to promote Daphnia growth.

Distribution of Aquatic Macrophytes in the Littoral of Lake Bohinj (Slovenia)

In alpine Lake Bohinj, which is an LTER site and a part of the national park, the occurrence and depth distribution of submerged and emerged aquatic macrophytes were analyzed. Nine submerged and one emergent macrophyte taxa were found in the lake: Myriophyllum spicatum, Chara virgata, Chara aspera, Potamogeton lucens, Potamogeton alpinus, Potamogeton crispus, Potamogeton perfoliatus, Potamogeton pusillus, Ranunculus circinatus with the synonym Batrachium foeniculaceum and the emergent species Phragmites australis. The depth of the vegetation zones was measured using a depth meter and their coordinates were recorded using a GNSS antenna with RTK receiver. These data were used along with a DEM of lake depths to accurately map the potential zone of macrophyte growth, which was based on the depths of macrophyte distribution. The potential zone of macrophyte growth consisted of 28 different transects and covered 240.14 ha of the lake. The macrophytes covered 5.55 ha. The most common and abundant species was M. spicatum. A significant difference in macrophyte cover was found between the south and north shores of the lake, with the south shore having more patches with a larger total area. A clear difference in macrophyte cover was also noted between the main inflow and outflow of the lake. The presence of macrophytes and their diversity varied in different parts of the lake due to differences in slope, depth and type of substrates.