Aloe Barbadensis Miller Research Articles

Green synthesis of silver nanoparticles via Aloe barbadensis miller leaves: Anticancer, antioxidative, antimicrobial and photocatalytic properties

Current work reports biogenic synthesis and physico-chemical characterization of silver nanoparticles (AgNPs) by using Aloe barbadensis miller leaves extract (ALE). The prepared biogenic AgNPs have been characterized physico-chemically by X-ray powder diffraction (XRD), UV-visible spectrometry, attenuated total reflectance infrared spectroscopy (ATR-IR), Atomic force microscopy (AFM) and Field emission- scanning electron microscopy (FE-SEM). The characteristic highest absorption peak at 439 nm confirmed the synthesis of AgNPs. Further, the biomedical and catalytic potential of AgNPs was investigated by their anticancer, antioxidative, antimicrobial, and photocatalytic properties. The anticancer potential of AgNPs was evaluated against the breast cancer (MCF-7) cells using MTT cytotoxicity assay. AgNPs proved to be anticancer even for the minimum concentrations of 10 µg/ml, and the anticancer efficiency was increased with increase in concentration. AgNPs showed 42.51% free radical scavenging activity due to their antioxidative nature. 0.3 M AgNPs showed maximum antimicrobial activity with 20 mm zone of inhibition against E. coli bacteria. Additionally, the AgNPs were tested for photocatalytic dye degradation of eosin, crystal violet, carbol fuchsin, methylene blue, and saffranine dyes with maximum % degradation 70.76%, 95.54%, 94.63%, 93.53%, and 90.12%, respectively. The study is unique as the biosynthesized silver nanoparticles by using Aloe barbadensis miller leaves extract shows promising anticancer, antioxidative, antimicrobial, and photocatalytic properties. Herein, the present study reveals the potential of biosynthesized AgNPs for improved therapeutic and catalytic applications.

Isolations andAntibacterial Tests of Aloe Vera Endophytic Bacteria (Aloe barbadensis miller) towards Staphylococcus aureus and Eschericia coli

Research in the field of microbiology regarding the identification of endophytic bacteria in aloe vera is still rarely carried out while many people in East Nusa Tenggara often use aloe vera to cure diseases. The purpose of this study was to identify the endophytic bacteria Aloe barbadensis miller and to determine its antibacterial ability against the growth of Staphylococcus aureus and Escsherichia coli bacteria. The research procedures included taking samples using purposive sampling method, isolating endophytic bacteria using streak plate method, testing the antibacterial potential against Staphylococcus aureus and Eschericia coli using the paper disc diffusion method. The results showed that endophytic bacteria found on aloe vera leaves were marked by the growth of several bacterial colonies on Murashige-Skoog media with different shapes and colors, then the colonies were separated and 8 isolates were obtained with isolate codes C1B, C2B, C3B, C4B, C5B, C6B, C7B and C8B. The results of the antibacterial potency test showed that isolates C1B, C3B, C5B and C8B had the potential to inhibit the growth of Staphylococcus aureus characterized by the presence of a clear zone on the surface of the media with the largest size being 20 mm in isolate C1B and the smallest clear zone being 7 mm in isolate C8B, while isolate C1B, C3B, C5B and C8B have the potential to inhibit the growth of Eschericia coli bacteria characterized by the presence of a clear zone on the surface of the media with the largest size of 10 mm in isolate C1B and the smallest clear zone of 7 mm in isolate C8B. The conclusion of this study is that endophytic bacteria have been identified in aloe vera and some of these bacteria have antibacterial abilities against the growth of Staphylococcus aureus and Escherichia coli bacteria.

Acute toxicity of the aqueous extract of Aloe barbadensis miller gel

Aloe vera (A v) is a medicinal plant used since ancient times. Itsmucilage contains no less than 75 active ingredients with multiple properties. The present research aims to study the acute toxicity of the aqueous extract of A v gel (AEAVG) and to determine itsno-toxic effect dose (NTED). After the aqueous extraction of A v gel, an acute toxicity study was conducted in Swiss mice for 14 days by oral administration of AEAVG in a single dose (0, 250, 500, and 1000 mg/kg BW). Acute toxicity evaluation affected mouse behavior, weight gain, food and water intake, serum biochemical parameters (transaminases, urea, and albumin), and organ histology (liver and kidneys). These results show that the NTED is lower than 500 mg/kg BW. AEAVG has toxic effects at doses equal to or greater than 500 mg/kg BW, raising the alarm about the therapeutic use of AEAVG. Bangladesh J. Sci. Ind. Res. 58(1), 19-34, 2023

Hygiene characterization of polypropylene nonwoven composites produced on loading bio synthesized silver nanoparticles using Aloe Barbadensis Miller plant extract

Maintenance of hygiene for textile articles and tools is obligatory for medical professionals with regard to their own as well as patients’ safety in health care sector. The demand is well satisfied for decades by applying macromolecules of antibacterial agents into the base material through either coating (laminating) or reinforcing (finishing) methods. However, foremost glitches observed with either of practices are; added stiffness to the article, restricted laundering ease, shortened service period on laundering, and uncomforting to the user particularly when element worn adjacent to the skin for a prolonged time interval. The issue can be remedied by transforming asepsis functional molecules to nano form rather than using in macro form. Additionally, such changeover also boosts up antibacterial activities of asepsis due to noteworthy increase in specific surface area. AgNPs colloidal were produced using 0.01 ​g, 0.05 ​g, and 0.1 ​g of AgNO3 salt along with natural herb Aloe Barbadensis Miller (Aloe vera) extract as a green reducing and capping agent. The optimal quality and stability of the colloidal were worked out by keeping AgNO3 feed level constant, and varying only the concentration levels of Aloe vera; 2.5 ​ml, 5.0 ​ml, 10 ​ml, and 15 ​ml. The best stable colloidal was achieved for 10 ​ml Aloe gel with 0.1 ​g AgNO3 salt. The synthesized colloidal were applied to the 45 GSM PP nonwoven material by exhaust technique. SEM and EDX techniques were used to confirm the presence of AgNPs as well as their uniform distribution with a little or no agglomeration deposition in the composite structure. Consistent and satisfactory antibacterial activities were noted even after 72 ​h of time lag against E. coli (gram-negative) and S. Aureus (gram-positive) bacteria for the best synthesized AgNPs/Aloe vera loaded composite. Higher Zone of Inhibition (ZOI) values were observed for the optimal composite in comparison to commercially established Amoxicillin chemical against both the species of bacteria.

Biodegradable curative film for medical application

An attempt has been made to produce a herbal based silk fibroin – chitosan biodegradable film. Herbs are used for wound healing purpose, silk fibroin for its remarkable bio-medical and mechanical properties and chitosan for its antimicrobial properties. Herbal extracts, such as Aloe barbadensis Mill, (Aloe Vera), Cissus quadrangularis (Pirandai), and Curcuma longa (Turmeric) have been used to impart wound healing effects. The physical and antimicrobial properties have been studied as per the standard methods. The cytotoxic reactivity of the test, and control sample are evaluated under an inverted phase contrast microscope based on the standard. Scanning electron microscope is used to study the morphology of prepared film for process versatility and highly specific surface area. The chemical compound assessments are studied by Fourier Transform Infrared. Microbial growth under the bio films is found to be absent, and test results show better activity against Escherichia coli and Staphylococcus aureus. The blended film shows none cytotoxic reactivity to fibroblast cells and test silk fibroin blended film shows slight cytotoxic reactivity to fibroblast cells after 24 h contact. The achievement of numerical more than 2 is considered as cytotoxic effect. Since the film samples achieve a numerical grade not greater than 2, the samples are considered as non cytotoxic. Microbial growth under the bio films is found to be absent and test results show better activity against Escherichia coli.

Qualitative and Quantitative Phytochemical Analysis of Aloe barbadensis Miller Leaf Extracts

The medicinal, folkloric, and other uses of aloe vera cannot be over-emphasized. In this study, we qualitatively and quantitatively analysed the phytochemical composition of Aloe vera. Fresh leaves of Aloe vera were extracted by percolation method using three different solvents: ethanol, diethyl ether, and distilled water. The extracts were analysed for important phytoconstituents using conventional qualitative and quantitative methods. The results showed that alkaloids, flavonoids, glycosides, cardiac glycosides, saponins, saponin glycosides, and tannins were present, while steroids, balsam, anthraquinones, and volatile oil were not detected. The quantitative analysis indicated a high concentration of alkaloids (31.067 g/100 g), tannins (25.66 g/100 g), and saponins (10.67 g/100g) while glycosides (0.060 g/100 g) had the least concentration. The result indicates the potential health and cosmetic benefits of Aloe vera as well as its potential benefits in the food industries

Extraction of anthraquinone and salicylic acid from Aloe barbadensis miller

The objective of the research was to identify suitable parameters for extraction of anthraquinone, and salicylic acid from Aloe (Aloe barbadensis Miller). The parameters were evaluated including ethanol concentration (99.5, 80.0, 50.0%), material/solvent ratio (50 g/50 mL, 50 g/100 mL, 50 g/150 mL, 50 g/200 mL), stirring speed (100, 200, 300, 400 rpm), temperature (40, 45, 50, 55°C), extraction time (10, 20, 30, 40 minutes). Optimum conditions for anthraquinone and salicylic acid extraction on a laboratory scale were the ratio of 1/2 (g/mL), 50°C, 20 minutes and 300 rpm for the ratio of material/solvent, extraction temperature, extraction time, stirring speed, respectively using ethanol 99.5% as solvent extraction. The maximum anthraquinone and salicylic acid content are 0.31 mg/mL and 2.03 mg/mL, respectively.

Fe de errata al artículo: Efecto de la incorporación de aloe vera (Aloe barbadensis Miller) en las características fisicoquímicas, bromatológicas y vida útil de un yogurt de leche de búfala

Montalvo-Puente, A.; Montero-Castillo, P.; Alvarez-Badel, B. 2022. Effect of incorporation of aloe vera (Aloe barbadensis Miller) on the physicochemical and bromatological characteristics and shelf-life of buffalo milk yogurt. Rev. U.D.C.A Act. & Div. Cient. 25(1):e2019. http://doi.org/10.31910/rudca.v25.n1.2022.2019 When the above article was first published, Alba Montalvo Puente’s affiliation was wrong. The correct one is Universidad Pontificia Bolivariana, Seccional Montería, Centro de Ciencia Básica, Programa de Ingeniería Agroindustrial, Grupo de investigación en desarrollo y aplicación de nuevos materiales (DANM). The editor of the Revista U.D.C.A Actualidad & Divulgación Científica apologies for this error.

ANTI-INFLAMMATORY, ANTIPYRETIC AND ANALGESIC ACTIVITIES OF CHLOROFORM EXTRACT OF ALOE BARBADENSIS MILLER IN ALBINO RATS

The pharmacological activities of the leaf gel of Aloe vera have been extensively evaluated. Gel and latex are two basic products of aloe leaves. Latex and gel contain biologically active components. Polysaccharides contained in the leaf gel attribute most of the health benefits like anti-inflammatory, pain and fever, associated with aloe vera. In the present study, chloroform extract of A. barbadensis at various concentrations was investigated for its anti-inflammatory, antipyretic and analgesic activities in albino rats. Twenty-four albino rats were randomly divided into three groups (control, standard and experimental group). Division of groups was the same for all activities. Control and standard groups contain 4 rats in each group whereas experimental group contains 16. All the rats in three groups were treated with carrageenan to induce oedema, Brewer’s yeast to induce pyrexia and acetic acid to induce pain. The control group was treated with normal saline for all the activities. Standard group rats were treated with the reference drug diclofenac for anti-inflammatory and analgesic activities and paracetamol for antipyretic activity. Experimental group rats were given chloroform extract of A. barbadensis with 50, 100, 200 and 400mg/kg concentration. The result showed a significant inhibition (98%) in oedema at 3rd hour at the dose of 400mg/kg as compared to control group. For antipyretic activity, there was a significant reduction (66%) in pyrexia at 4th hour at the dose of 50mg/kg as compared to control group. And in analgesic model a significant reduction (64%) in the writhing at the dose of 400mg/kg as compared to control group. These results demonstrated that the chloroform extract of Aloe barbadensis miller have anti-inflammatory, antipyretic and analgesic activity and suggested its inhibitory actions on inflammation, fever and pain.

Evaluation of Aloe barbadensis Miller and Musa x paradisiaca as Internal Curing Agents in Concrete

Curing is essential for enhancing the durability and strength of concrete. Researchers found that a lack of conventional curing in earlier days leads to a loss of economy over the years after construction finishes. Self-curing concrete is a contemporary type of concrete that holds water, prevents loss of moisture from the surface, and facilitates self-curing. The existing chemical admixture for self-curing, polyethylene glycol (PEG), is expensive. Hence, in this research, bio admixtures such as Aloe barbadensis miller and Musa x paradisiaca were tried as self-curing agents and compared with the performance of PEG. The functional groups of such bio admixtures match with those of PEG. The results show that the fresh and hardened properties of M30 concrete are better than the conventionally cured concrete and PEG added to concrete. The optimized percentages of admixtures are 0.25% for Aloe barbadensis miller, 1% for Musa x paradisiacal, and 0.5% for PEG, improving the compressive strength by 23.3%, 1.7%, and 4.5%, respectively. Similarly, split tensile and flexural strength have been enhanced up to 4.24 MPa and 15.05 MPa for Aloe barbadensis miller, and 3.82 MPa and 13.65 MPa for Musa x paradisiacal. The characterization studies’, such as XRD (X-ray diffraction), SEM (scanning electron microscope), and EDX (energy dispersion analysis), results show the early formation of hydrated products, such as CSH and CH, after 7 days of curing with an optimized mix. Of the two plant extracts, Aloe barbadensis miller performed better than Musa x paradisiacal and water-cured concrete.

Calcium- and Magnesium-Enriched Organic Fertilizer and Plant Growth-Promoting Rhizobacteria Affect Soil Nutrient Availability, Plant Nutrient Uptake, and Secondary Metabolite Production in Aloe vera (Aloe barbadensis Miller) Grown under Field Conditions

This work investigates the effects of an organic fertilizer enriched in Ca and Mg and two bacterial inoculants, applied alone and in combination, on soil fertility, plant growth, nutrition, and production of secondary metabolites, namely, acemannan and total phenolic compounds (TPCs), by Aloe vera (Aloe barbadensis Miller), under field cultivation. The first inoculum consisted of five native bacterial strains (Pseudomonas sp., Enterobacter sp., and three strains of Pantoea sp.), characterized in vitro as putative plant growth promoters, isolated from local organic farming fields of Aloe vera. The second inoculant was a commercial product (BACTILIS-S and HUMOFERT) and consisted of three Bacillus species: B. pumilus, B. amyloliquefaciens, and B. subtilis. The organic fertilizer (HUMO-CAL M-8O) was a mixture of humic and fulvic acids, with an additional CaCO3 (40% w/w) and MgO (4% w/w). The most significant increase in the content of acemannan and TPCs was detected under single application of the organic fertilizer, which was linked to enhanced concentration of Mg and Ca in the leaf gel. The concentration of acemannan tended to be increased with the combined application of the organic fertilizer and microbial inoculants. TPCs were significantly increased in both single and combined treatments, seemingly related to Fe concentration in the leaf rinds.

Growth and Yield of Aloe Vera (L.) Burm.f. As Influenced By Organic Manures and Chemical Fertilizers

Influence of organic manures (10 ton ha-1), nitrogen (50 and 100 kg ha-1), phosphorus (10 and 20 kg ha-1) and potassium (30 and 60 kg ha-1) on the growth and yield of Aloe vera (L.) Burm.f. (Aloe barbadensis Mill.) were evaluated. Experiment was conducted in a randomized design replicated thrice with eleven treatments with NPK and without NPK fertilizers. Highest length of leaf (18 cm), highest leaf area (23.40 cm2plant-1), highest leaf area index 0.06 were observed in ACI10ton ha-1plus N50P10K3O kg ha-1 treatment, highest fresh weight (78.23gplant-1) and highest dry weight (9.58 gplant-1) of plant biomass were observed in GTS 10ton ha-1 treatment. Highest concentration of N (2.69%) and protein content (16.84 %) in the leaf were recorded in GTS10 ton ha-1 treatment. Results revealed that the best growth performance and yield, and protein content in the leaf of Aloe vera was observed in GTS10 ton ha-1 treatment. Dhaka Univ. J. Biol. Sci. 31(2): 203-212, 2022 (July)

Jatropha multifida L stem sap gel versus Aloe vera gel to post-gingivectomy healing process

Introduction: Gingivectomy is cutting the gingiva during a gingival enlargement condition that causes an open wound in the gingiva. Jatropha multifida L gel contains active ingredients of alkaloids, flavonoids, saponins and tannins, which are thought to accelerate the wound healing process, but its use in dentistry is limited. The objective of this research is to determine the effectiveness of the application of Jatropha multifida L stem sap gel in the wound healing process post-gingivectomy in Wistar rats (Rattus novergicus) compare with Aloe vera gel (Aloe Barbadensis Miller) by using parameters the number of fibroblasts, blood vessels and the density of collagen fibers Methods: Rats were divided into 4 groups, namely group K (Aloe vera gel), P1 (Jatropha multifida L stem sap gel 2,5%), P2 (Jatropha multifida L stem sap gel 5%) and P3 (Jatropha multifida L stem sap gel 10%). Each group was given the gel twice a day in the wound area; then the wound was observed microscopically by Haematoxylin Eosin staining to count the number of fibroblasts and blood vessels also Masson Trichrome staining to observe the density of collagen fibers on day 3 and 7. Results: The data obtained were statistically analyzed using the One Way Anova, Dunnet and Kruskal-Wallis test. One Way Anova test shows there is a significant difference (p<0.05) in the number of fibroblasts, and the Dunnet test shows there is a significant difference (p<0.05) in the number of fibroblasts in Jatropha multifida L stem sap gel group 2.5% and 5 % on day 3. One Way Anova test shows no significant difference (p>0.05) in the number of blood vessels. The Kruskal-Wallis test shows no significant difference (p>0.05) in collagen fiber density. Conclusion: Jatropha multifida L Stem Sap gel has the same potential as Aloe vera gel in accelerating wound healing after gingivectomy in Wistar rats.

Comparative Evaluation of Antimicrobial Efficacy of Aloe Barbadensis Miller, Curcuma Longa and Azadirachta Indica with Sodium Hypochlorite: An Invitro Study

Context: The thorough removal of microflora, debris, and irritants from the root canal system is necessary for root canal treatment to be effective. Herbal or natural products are becoming more popular recently as a result of their strong antibacterial activity, biocompatibility, anti-inflammatory, and anti-oxidant properties
 Aims: To compare and evaluate antimicrobial efficacy of herbal extracts Aloe barbadensis miller, Curcuma longa and Azadirachta indica with 2.5% sodium hypochlorite against the Enterococcus faecalis .
 Settings and Design: Forty muller Hinton agar plates were taken as sample and were grouped into 4 groups as Group I: 2.5% NaOCl ,Group II: Curcuma longa extract ,Group III: Azadirachta indica extract, Group IV: Aloe barbadensis miller.
 Methods and Material: The agar plates were inoculated with strains of E.faecalis (ATCC29212) and test solutions were placed in agar wells in group 1-4. The agar plates were incubated at 37 0C for 72 hours. After incubating, the agar plates were examined for zone of inhibition for assessing the degree of susceptible or resistance of test organism.
 Statistical analysis used: Statistical analysis was performed using Oneway Anova test with statistical significance at P < 0.05.
 Results: It was shown that Curcuma longa was highly efficient similar to 2.5%NaOCl
 Conclusions: Curcuma longa extract has a significant antimicrobial efficacy against Enterococcus faecalis similar to 2.5% sodium hypochlorite
 Key Messages: Herbal irrigants are best alternative to gold standard chemical endodontic irrigants. With their increase in antimicrobial properties, they aid in complete removal of endodontic microorganisms.

Role of Medicinal Herbs in Periodontal Therapy: A Systematic Review.

The use of medicinal herbs to prevent gingival and periodontal diseases has become increasingly popular due to their anti-inflammatory and antioxidant properties. This systematic review aims to provide the current literature to validate the traditional use of medicinal herbs in the management of gingival and periodontal diseases. An online literature search was conducted to identify research papers published from 2010 to 2022 in three major scientific databases, PubMed, Scopus, and Web of Science, in June 2022. Original research studies, case reports, and systematic reviews on medicinal plants' application in oral health care were selected to be included in this systematic review. Only high-quality articles identified in the quality assessment were included for evidence synthesis. Initial keyword research yielded 726 free-text articles published between 2010 and 2022. Of these, 14 articles (8 research papers and 6 reviews) were included for evidence synthesis. The review's findings indicate that the antibacterial property of medicinal plants is due to their alkaline nature and prevents plaque and calculus formation by maintaining acid-alkali balance in saliva. Various parts of medicinal plants help maintain periodontal health. Glycyrrhiza glabra, Ficus religiosa, and Plantago major effectively inhibit primary plaque colonizers and periodontal pathogens. Medicago sativa, Aloe barbadensis Miller, and Trifolium pratense have excellent applications in treating periodontal diseases. Mangifera indica, Pongamia pinnata, the husk of Cocos nucifera, the root of G. glabra and Curcuma longa, leaves of Psidium guajava and Azadirachta indica, fruits of Citrus medica and Punica granatum, Ocimum Moringa oleifera extract, and pomegranate peel extract can serve as a promising alternative in managing chronic gingivitis. The anti-inflammatory, antioxidant, antibacterial, and astringent action of extracts obtained from various parts of medicinal plants make them effective in reducing gingival and periodontal diseases. Herbal medicine may be a viable alternative to contemporary pharmaceuticals as an adjuvant to scaling and root planning procedures.

Evaluation and formulation of antiseptic herbal hand wipes

Background Wet wipes have been commercially used for various purposes, to clean hard surfaces such as floors or kitchen surfaces including personal cleansing. Wet wipes were impregnated with a synthetic lotion for cleaning the skin, these wipes are hygienic because they are disposable and are normally discarded after their first use. Commercially available antiseptic Wet wipes have strong antimicrobial agents Triclosan and other chemicals. The present objective is to alternate the strong antimicrobial agents by using Herbal formulation in Wet wipes. With Aloe barbadensis miller gel and essential oils based antimicrobial formulation was used to prepare antiseptic wet wipes. Methods Antimicrobial (Agar well diffusion method), antioxidant (DPPH assay), toxicity studies (using Zebra fish embryo) were carried out in this study. Results The 0.5-2µg/ml of essential oil showed promising antimicrobial activity against standard routine bacterial pathogens, and also for Mycobacterium smegmatis wild strains. 100µg/ml of Tea tree oil and cinnamon oil were toxic to zebrafish embryo. However, sub-lethal doses of oils is (100 μg/mL) against Zebrafish embryo. Conclusion Thus, an eco-friendly, safe, low-cost herbal hand wipes are formulated for human use for prevention and protection against microbial infection and for maintaining personal hygiene.

Demonstration of effectiveness: Plant extracts in the tuning of BiOX photocatalysts' activity

The use of plant extracts, recently, has been extended to the field of photocatalysis and is reported as a “greener” route. It is called “greener” because it uses plant extracts as the main component which doesn’t produce any toxic compound. The present work deals with the synthesis of bismuth oxyhalides (Cl, Br, I) with three different plant extracts, namely, Camellia sinensis, Azadirachta indica and Aloe barbadensis miller by a precipitation method for the removal of phenol under visible light. To clarify the effect of each plant extract, BiOX reference samples were prepared without plant extract. The samples were characterized for their structural, morphological, chemical and optical properties using XRD, SEM, FT-IR and DRS. Plant extracts, mainly, acted as structural and shape-tailoring agent as evident through a sharp decrease in the crystallite size and transformation of the plates-like morphology of BiOX. The photocatalysts BiOI with Azadirachta indica and BiOBr with Camellia sinensis extract removed 87 % and 82 % of phenol, respectively, within 240 min. The role of organics coming from the plant extracts is discussed and significant correlations with the structural and optical properties were made to unfold the mystery of activity enhancement of the plant extract mediated BiOXs.

Formulation and Antimicrobial Evaluation of A Novel Anti-Acne Herbal Hydrogel

Objective: The present study evaluates anti-microbial effect of hydrogel formulated with the extracts of some indigenous drugs against Propionibacterium acne.
 Material and Method: Procured Manjishtha (Rubia Cordifolia L.), Shalmali (Salmalia malabarica (DC. Schott & Endl) Schott & Endl.), Daruharidra (Berberis aristate DC), Aloe vera (Aloe barbadensis Miller), Yashthimadhu (Glycyrrhiza glabra L). Extracts of all drugs except Aloe vera were collected by hydroalcoholic extraction using the Soxhlet apparatus. Aqueous extract of Aloe vera was collected by drying method. Two types of hydrogels were prepared with the extracts of these drugs, one with 5% extract and the other with 10% extract. Propionibacterium acne strains were collected from CSIR-Imtech Chandigarh. Culturing of bacteria was done by using nutrient broth. Nutrient agar was used for preparing the culture media. The antibacterial efficacy of the hydrogels was evaluated by comparing them with standard gentamycin. Result: The prepared hydrogel showed an anti-bacterial effect, but it is comparatively less when compared with the standard gentamycin. Conclusion: The prepared gel has anti-microbial activity. 10% hyrdogel showed better results than 5% hydrogel.

Dissolution Effectiveness of Moringa Leaf Pills (Moringa Oleifera L.) and Aloe Vera (Aloe Barbadensis Miller)

Oral preparations with level solubility tall so speed dissolution substance active from form preparations Becomes more fast, anyway with preparations with speed dissolution low so speed dissolution substance active from form preparations Becomes slow, so rate absorption drug more slow as well as produce bioavailability low. Materials potion drug traditional for example from ingredient plant natural around which not yet experience processing whatever. Ingredient nature used _ as drug traditional in study this is plant leaf moringa and tongue crocodile. Leaf Moringa (Moringa oleifera L.) has various type effect therapy like antimicrobial, anticancer, anti-inflammatory, effect antidiabetic as well as as antioxidants. Compound chemical in the leaves _ moringa that is there is polyphenols, acids phenolics, flavonoids, glucosinolates, alkaloids, vitamins, acids phenolics, isothiocyanates, tannins and saponins. Plant tongue crocodile (Aloe barbadensis Miller). kindly chemistry, tongue crocodile consists over 90% water and 4% carbohydrates, the rest consists of minerals, 17 kinds amino acids. Gel found on the tongue crocodile there is content substance antibacterial as well as possible antifungal _ stimulate tiroblast . Study this aim for knowing effectiveness dissolution pill from leaf moringa and tongue crocodile with use method study experimental. Result of study this yield extract leaf moringa and tongue crocodile obtained on the leaves moringa by 6.38% and tongue crocodile 6.268%. Assessment test preparations pill Fulfill condition uniformity weight and fulfill dissolution test conditions because time the required crush for 30 minutes.

Phenolic profile and biological activities of Aloe barbadensis (Miller) from western Algeria

Phenolic profile and biological activities of Aloe barbadensis (Miller) from western Algeria