In this study, we composited mineralized collagen and magnesium-calcium alloy by freeze-drying, followed by dip-coating PMMA bone cement to enhance the composite of mineralized collagen and magnesium-calcium alloy. In vitro degradation test was performed to observe the pH and weight loss of the material. The contact angle test was used to detect the hydrophilicity of the material. Subsequently, MC3T3-E1 were used to assess cell biocompatibility In vitro by cell adhesion, cytotoxicity, alkaline phosphatase, alizarin red staining, and cytoskeleton. The results showed that the pH changes of the PMMA/NHAC/Mg–Ca was slower than that of the Mg–Ca , and the weight loss rate at 7 d and 14 d were lower than that of the Mg–Ca (P < 0.05) in degradation test. Wettability experiment showed that PMMA/NHAC/Mg–Ca was a hydrophilic material and Mg–Ca was a hydrophobic material (P < 0.05). In vitro cell experiments, the PMMA/NHAC/Mg–Ca had more cell adhesion than Mg–Ca and more synapses were connected to others. In the cytotoxicity experiment, the cell proliferation lever of PMMA/NHAC/Mg–Ca was higher than that of Mg–Ca at each time point (P < 0.05). In the 7 d alkaline phosphatase experiment, the PMMA/NHAC/Mg–Ca showed higher ALP activity than the Mg–Ca (P < 0.05), and in the alizarin red experiment at 14 d and 28 d, there were more obvious calcified nodules and mineralized area. After 1 day of culture in the PMMA/NHAC/Mg–Ca extract, the cells showed a clearer and more complete cytoskeletal structure and better cell morphology. In conclusion, PMMA/NHAC/Mg–Ca orthopedic implants had a better hydrophilicity, cytotoxicity and osteogenic ability, besides with a slower rate of degradation, and could be implanted in animals for further research, which were expected to be used for the repair of clinical bone defects.
Read full abstract