Lake systems play a pivotal role in the global carbon cycle, yet the biogeochemical processes in alkaline and saline lakes have received little attention. In this study, we present a comprehensive analysis of δ13Ccarb and δ18Ocarb records from Lake Chagan in the Otindag Sandy Land, located in North China, spanning approximately 7000 years. Our aim is to explore how the lacustrine inorganic carbon cycle responded to both climate change and human activity. The findings reveal a notable highstand in Lake Chagan around 6.9–6.0 cal kyr BP, followed by a gradual regression leading to an unstable state over the past 1.6 cal kyr BP. The hydroclimate variations inferred from Lake Chagan align with other regional records, suggesting that long-term East Asian summer monsoon (EASM) precipitation trends were influenced by decreasing insolation and variations in solar irradiance on a centennial timescale. The sources of dissolved inorganic carbon (DIC) in lake water generally mirrored regional hydroclimate changes since the middle Holocene. Our evidence indicates that during periods of strong EASM activity, more allochthonous organic matter was converted to lake water DIC, coinciding with high vegetation cover and intense soil erosion. While hydroclimatic changes significantly impacted the lacustrine carbon cycle, the isotopic evidence also suggests substantial anthropogenic influences post-1.6 cal kyr BP, particularly in terms of the input of allochthonous organic matter into the lakes of the Otindag Sandy Land. These findings underscore the potential for anthropogenic-driven terrestrial carbon cycle feedback to global climate change over millennia.