Allium Sativum Oil Research Articles

Clinical and Cost-effectiveness of Allium sativum oil versus Mineral Trioxide Aggregate in primary molars pulpotomy in a group of Egyptian children: A Randomised Clinical Pilot Study.

Clinical and Cost-effectiveness of Allium sativum oil versus Mineral Trioxide Aggregate in primary molars pulpotomy in a group of Egyptian children: A Randomised Clinical Pilot Study.

Antimicrobial Activity and Phytochemical Composition of Allium sativum Bulb Extracts against Selected Gram-Negative Bacteria

As a result of increasing antibiotics resistance due to multidrug resistance leads to the use of the medicinal plants as alternative antimicrobial agents. The study aimed to investigate the antimicrobial and phytochemical properties of Allium sativum bulb extracts against some selected gram-negative bacteria isolates. Allium sativum oil was extracted using Soxhlet extraction procedures; Clinical bacterial isolates were collected from pathology department of General Hospital Potiskum in Yobe state. Isolates were identified using standard methods. Antibacterial activity of different concentrations (20, 40, 60, 80 and 100) % of oil extract of Allium sativum bulb were investigated against five clinical bacterial isolates including, Proteus vulgaris, Pseudomonas aeruginosa, Escherichia coli, Shigella boydii and Klebsiella oxytoca.. The oil extract of the bulbs showed antimicrobial activity against all five clinical isolates. MICs and MBCs were evaluated by broth dilution method. The minimum inhibitory concentration (MIC) values for all the three oil extracts ranged between 0.5 and 1.0%, while the minimum bacterial concentration (MBC) values ranged between 1.0 and 1.5%. Important bioactive constituents were present in the different oil extraction method; alkaloids (for ethanol extracts, 19.04±0.04, for hexane extracts, 20.04±0.14 and for chloroform extracts, 20.04±0.07) and 0.51±0.01were detected for sterols in all the three oil extraction methods while others such as flavonoids, saponins, tannins and phenols were not detected. The yield extracts of the oil extract of the bulbs, for hexane were 12.6%, chloroform, 15.2% and ethanol, 16.2%, indicating that ethanol was the better of the three solvents used. The potential applications of Allium sativum oil extracts as natural antimicrobial agents call for the importance of further investigation into their nutritional potential.

Post-operative pain after vital pulpotomy of primary molars using allium sativum oil versus mineral trioxide aggregate

Aim: To evaluate clinical and radiographic performance of Allium Sativum oil dressed in Zinc Oxide versus Mineral trioxide aggregate as pulp dressing agent in pulpotomized lower primary molar teeth. Methodology: This randomized pilot clinical trial included 30 lower primary molars indicated for vital pulpotomy and randomly allocated into two equal groups (n=15): Group (I): pulpotomy using A. Sativum oil dressed in ZnO and Group (II): pulpotomy using Mineral trioxide aggregate as pulpotomy agent. All molars were covered with stainless steel crowns and clinical and radiographic assessment was done at 3, 6, 9 and 12 months interval by help of predetermined criteria. Results: Overall clinical success rate of Group (I) and Group (II) at the end of 12 months follow-up was (93.3%) and (100.0%) respectively. There was no statistical significance difference between two groups regarding overall clinical success rate. The overall radiographic success rate at the end of 12 months was (40.0%) and (100.0%) respectively. There was a statistical significant difference regarding overall radiographic success rate at 9 and 12 months follow-up. The overall success rate of Group (I) and Group (II) at the end of 12 months was (40.0%) and (100.0%) showing a statistically significant difference between the two groups.

The clinical and radiographic evaluation of Allium sativum oil (garlic oil) in comparison with mineral trioxide aggregate in primary molar pulpotomy.

Background:Mineral trioxide aggregate (MTA) is known as a common biomaterial for pulpotomy of primary molars, with limitation including high cost, difficult handling, and long setting time. This study was conducted to compare the clinical and radiographic success rate of Allium sativum oil and MTA in pulpotomy of primary molar teeth.Materials and Methods:This randomized, controlled, clinical trial was performed on a group of children with at least two symptom-free primary molars requiring pulpotomy. A total of 90 teeth were randomly assigned into two groups: After pulp amputation and hemostasis, Group I received MTA as medication in the pulp chamber and Group II received Allium sativum oil over the pulp stumps. Pulp chamber was then filled with reinforced ZOE paste (zinc oxide eugenol) and teeth were restored using stainless steel crowns. The follow-up scheme of 6, 12, and 18 months was set, and teeth evaluation was conducted by a calibrated pediatric dentist based on the modified criteria proposed by Zurn and Seale. The results were analyzed using the generalized estimating equation analysis with the P = 0.05 as the level of significance.Results:The clinical success rate was 100% in both groups after all follow-ups. The radiographic success rate was, however, 91.1% after 6.75. 6% after 12 and 18 months in Group II while it was 95.6% after 6, 91.1% after 12 and 18 months in Group I in those follow-up points. The difference between the two groups was not statistically significant.Conclusion:According to the results, Allium sativum oil has a high clinical and radiographic success rate comparable to those of MTA.

BIOLOGICAL ACTIVITIES OF ESSENTIAL OILS FROM ANETHUM GRAVEOLENS L. AND ALLIUM SATIVUM L. FOR CONTROLLING TETRANYCHUS TRUNCATUS EHARA AND TETRANYCHUS URTICAE KOCH

ABSTRACT The essential oils of Anethum graveolens L. and Allium sativum L. were investigated for the biological activities against Tetranychus urticae Koch and Tetranychus truncatus Ehara. The results indicated that A. sativum oil at 20% (v/v) had high contact toxicity (73.50% mortality) on adult females of T. truncatus. The LC50 value was 12.71% for 24 h after treatment. Eggs of both spider mites were also susceptible to 5-20% and 20% concentrations of A. sativum and A. graveolens oil, respectively. Allium sativum oil caused 97-100 and 84% mortality against T. truncatus and T. urticae eggs with LC50 values of 1.62 and 11.58% at 7 d after exposure, respectively. In repellency test, A. graveolens oil at 5-20% concentrations showed a high percentage of repellency on T. truncatus (62-96%) and T. urticae (98-100%) during 1-5 h after treatment. On the other hand, 5-20% and 15-20% concentrations of A. sativum oil appeared high percentage of repellency on T. truncatus (98-100%) and T. urticae (86-100%) during 1-5 h after treatment, respectively. In a fumigant toxicity test, A. sativum and A. graveolens oils were toxic to both spider mites. Allium sativum oil (LC50 = 4.94 µL/L in air) and A. graveolens oil (LC50 = 13.67 µL/L in air) were toxic to adult females of T. urticae whereas T. truncatus adult females were responsible to A. graveolens oil (LC50 = 5.24 µL/L in air) and A. sativum oil (LC50 = 5 µL/L in air). The results suggested that essential oils of A. sativum had potential for ovicidal activity on T. truncatus and T. urticae and acaricidal activity on T. truncatus. Both oils showed repellent activity against both spider mites. Oils of A. sativum and A. graveolens were toxic in fumigant effect on both spider mites. Their biological activities could be useful in promoting of integrated mite management.

Clinical and Radiographic Evaluation of the Efficacy of Formocresol, Allium sativum Oil, and Aloe barbadensis Gel as Pulpotomy Medicaments in Primary Molars: A Randomized Controlled Trial.

AimThe aim of the study was to clinically and radiographically evaluate the efficacy of formocresol, Allium sativum oil, and Aloe barbadensis gel as pulpotomy medicaments in primary molars.Materials and methodsEighty-two primary molars indicated for pulpotomy were included in the study. The molars included were divided into three groups by simple random sampling, and treatment was done with formocresol as control, comparing it with Aloe barbadensis gel and Allium sativum oil.ResultsThe teeth were then followed up for 6 and 12 months clinically and radiographically. Chi-square test was used to compare the clinical and radiographic results between the groups. McNemar test was used to compare the clinical and radiographic results at 6 and 12 months. The results revealed that there was no significant difference between the groups when comparing the experimental groups to the control.ConclusionThe study shows that Aloe barbadensis gel and Allium sativum oil may be used as an alternative to formocresol as pulpotomy medicaments.How to cite this articleAbirami K, Ramkumar H, Senthil D. Clinical and Radiographic Evaluation of the Efficacy of Formocresol, Allium sativum Oil, and Aloe barbadensis Gel as Pulpotomy Medicaments in Primary Molars: A Randomized Controlled Trial. Int J Clin Pediatr Dent 2020;13(5):518–522.

Efficacy of Allium sativum oil to alleviate tebuconazol-induced oxidative stress in the liver of adult rats

The present study focused on the protective efficacy of Allium sativum oil (ASO) against tebuconazol (TEB)-induced oxidative stress in the liver of adult rats. Thirty-two rats were randomly divided into four groups of eight each: group I served as control rats, group II was treated with TEB (100 mg/kg bw), group III received ASO (5ml/kg bw). The animals of group IV were treated with TEB and ASO, during 4 weeks. The obtained results showed that TEB induced a significant change of some hematological parameters, including red blood cells (RBC), haemoglobin content (Hb), haematocrit (Ht), white blood cells (WBC) and platelet (Plt) compared to the control group. Moreover, while the total cholesterol levels and the activities of aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), lactate dehydrogenase (LDH) and γ-Glutamyltranspeptidase (γGT) significantly increased due to TEB administration, the concentrations of plasma total protein, albumin and triglyceride considerably decreased. Furthermore, the exposure to TEB significantly increased the malondialdehyde (MDA), protein carbonyl (PCO) and advanced oxidation protein products (AOPP) levels and decreased glutathione peroxidase (GPx), superoxide dismutase (SOD), catalase (CAT) and glutathione-S-transferase (GST) activities in the hepatic tissues. The results were confirmed by the histological impairments. Besides, the co-administration of ASO improved the status of all studied parameters. Therefore, our investigation revealed that ASO had protective effects against TEB-induced liver injury, which could be attributed to its phenolic compounds.

Comparative Evaluation of Pulpal Response to Tri-antibiotic Paste and Allium Sativum with Formacresol as Pulpotomy Medication in Primary Teeth:An in vivo Study

Background:-To evaluate and compare Pulpal Response to Tri-antibiotic Paste and Allium Sativum with Formacresol as Pulpotomy Medication in Primary Teeth. Materials and Methods:-30 children in age group 7-9 years have bilateral primary teeth indicated for orthodontic serial extraction .children divided into two groups, (group I) included(15) children had bilateral primary teeth, the right side tooth treated with tri-antibiotic past (3 mix) (group IA) and left side tooth treated by formacresol (group IB) and (group II) included (15) children had bilateral primary teeth, the right side tooth treated with allium sativum oil (A. sativum) (group IIA) and left side tooth treated by formacresol (group IIB) . Each tooth was treated with standard pulpotomy procedure. . Patients were recalled after 15 and 30 days’ time interval as a part of orthodontic treatment. Teeth in each group were extracted and were subjected to decalcification procedure for histopathological evaluation. Results:- In group(I A) Nearly normal pulpal architecture was observed. Another finding common to all experimental groups was the presence of inflammatory processes of different levels of severity. Moderate amount of inflammatory cell infiltration was noted about 50-60% in cases after 15 and 30 days. In addition the results of group (IB), (IIB) there was pulpal inflammation was prominently observed in all cases treated with formacresol. After 15 days about 33.3% of cases showed severe inflammation with lymphocytes and foamy macrophages while 66.6% showed moderate inflammatory infiltration. By the end of 14 days cases with severe pulpal inflammation were about 46.6%. Also, in group (IIA) there was pulpal inflammation was prominently observed in all cases treated with formacresol. After 15 days about 33.3% of cases showed severe inflammation with lymphocytes and foamy macrophages while 66.6% showed moderate inflammatory infiltration. By the end of 30 days cases with severe pulpal inflammation were about 46.6%.Conclusion:- 1-Odontoblast remained vital in most cases treated with Both Tri-antibiotic paste and A. sativum . 2-Interal root resorption found in most specimens treated with formacresol .Recommendations: Further clinical studies with long time evaluation periods should be done to investigate the effects of the three medicament on pulp tissues of primary teeth .

Evaluation of Post-operative Pain After Vital Pulpotomy in Primary Molars Using Allium Sativum Oil Versus MTA

Evaluation of Post-operative Pain After Vital Pulpotomy in Primary Molars Using Allium Sativum Oil Versus MTA

Clinical and Radiographic Evaluation of Allium sativum Oil (Garlic Oil) in Comparison with Formocresol in Primary Molar Pulpotomy.

Background:Given the side effects of formocresol as a common substance in dental pulpotomy, its substitution with a safe substance seems mandatory.Aim:To compare the clinical and radiographic success of Allium sativum oil and formocresol in the pulpotomy of the primary molars.Materials and Methods:A randomized, split-mouth, double-blind, controlled clinical trial was conducted on children with at least two asymptomatic primary molars requiring pulpotomy. A total of 90 teeth were examined in this study. They were randomly divided into two groups: the A. sativum (ALL) group (n = 45) and the formocresol (FC) group (n = 45). After pulpotomy, A. sativum oil was applied to the radicular pulp in the ALL groups and formocresol in the FC group. The pulp chamber was then filled with reinforced Zinc oxide Eugenol and finally restored by a stainless steel crown. Three and six months after the intervention, the teeth were evaluated by a colleague based on a modified criteria system. The results were analyzed using Wilcoxon’s test.Results:The clinical success rate was 100% in both groups after 3 and 6 months. The radiographic success rate in the ALL group and the FC group after 3 and 6 months was 82.2% and 80% and 88.9% and 84.4%, respectively. The difference between the two groups was not statistically significant (P = 0.46).Conclusion:According to the results of this study, A. sativum oil can be used in the pulpotomy of the primary molars.

Histological Evaluation of Allium sativum Oil as a New Medicament for Pulp Treatment of Permanent Teeth.

The objective of this study was to evaluate the histo pathology effects of two medicaments Allium sativum oil and formocresol on the remaining pulp tissue of the permanent teething children. A total of 18 premolars were included in this study. Two sound premolars were extracted and subjected to histological examination to show the normal pulp tissue. Pulpo tomy procedure was performed in the rest of the remaining 16 premolars; half of them using Allium sativum oil and the rest of the tested premolars were medicated using formocresol and all were sealed with suitable restoration. Then, premolars extracted at variable intervals (48 hours, 2 weeks, 1 month, 2 months), stained using hemotoxylin and eosin etain (H&E) and prepared for histopathology examination. Histological evaluation seemed far more promising for Allium sativum oil than formocresol. Histological evaluation revealed that teeth treated with Allium sativa oil showed infammatory changes that had been resolved in the end of the study. On the contrary, the severe chronic infammation of pulp tissue accompanied with formocresol eventually produced pulp necrosis with or without fibrosis. In addition, pulp calcification was evidenced in certain cases. Allium sativum oil is a biocompatible material that is compatible with vital human pulp tissue. It offers a good healing potential, leaving the remaining pulp tissue healthy and functioning.

Bacteriological evaluation of Allium sativum oil as a new medicament for pulpotomy of primary teeth.

Objective:To compare the effects of Allium sativum oil and formocresol on the pulp tissue of the pulpotomized teeth.Materials and Methods:Twenty children were selected for this study. All children had a pair of non-vital primary molars. A sterile paper point was dipped in the root canals prior to the mortal pulpotomy. These paper points were collected in transfer media and immediately transported to the microbiological lab to be investigated microbiologically (for Streptococcus mutans and Lactobacillus acidophilus). Then the procedure of mortal pulpotomy was performed. After 2 weeks, the cotton pellets were removed and sterile paper points were dipped in the root canals for microbiological examination. Then comparison between the count of bacteria before and after treatment was conducted. Statistical analysis was performed using independent t-test and paired t-test at the significance level of α = 0.05.Results:After application of both medicaments, there was a marked decrease in S. mutans and L. acidophilus counts. The difference between the mean of log values of the count before and after the application was highly significant for both medicaments (P < 0.05); however, better results were obtained when A. sativum oil was used.Conclusion:A. sativum oil had more powerful antimicrobial effects than formocresol on the bacteria of the infected root canals.

Assessment of the potential of Allium sativum oil as a new medicament for non-vital pulpotomy of primary teeth.

Objective:The objective of this study was to compare the clinical and radiographic effects of Allium sativum oil and formocresol in nonvital pulpotomy in primary teeth.Materials and Methods:Twenty children ranging in age from 4 to 8 years were included in the study. In every one of those children, pulpotomy was indicated for the primary molars. Pulpotomy procedure was performed and the radicular pulp tissue of one molar was capped with A. sativum oil in a cotton pellet while the other molar was capped with formocresol. The teeth were evaluated clinically and radiographically before and after 6 months using standard clinical and radiographic criteria. Statistically, these results revealed significant difference between the radiographic findings of nonvital pulpotomy in primary molars with the two medicaments. Statistical analysis was performed using independent t-test and paired t-test at the significance level of α = 0.05.Results:A. sativum oil has potent antibacterial properties that enable it to combat intracanal microbes in the infected pulp of primary molars. Better results were obtained when A. sativum oil was used.Conclusion:A. sativum oil had more powerful effects than formocresol on the infected pulp of primary nonvital molars.

Body Surface Changes in Gastrointestinal Helminthes Following in vitro Treatment with Allium sativum Oil

A scanning electron microscopic study was undertaken to assess, for the first time, whether Allium sativum oil (ASO) extract had any effect on helminthes’ body surface following 24 h incubation in vitro. Two species of gastrointestinal helminthes, of most economic significance in sheep, Haemonchus contortus and Moniezia expansa were collected from naturally infected sheep slaughtered in Cairo abattoir, then exposed to 10, 50, 100 μg/ml ASO. The body surface of these helminthes was observed to be affected and altered by ASO. These changes were concentration dependent, and consisted of destructive alterations and deformity in the cuticle of H. contortus and also in the tegumental architecture of M. expansa. The current study confirmed that an alternative, effective and natural anthelmintic could be developed using garlic.

Effect of Garlic and Eucalyptus oils in comparison to Organophosphatinsecticides against some Piercing-Sucking Faba bean insect Pests and natural enemies populations

The efficacy of garlic Allium sativum oil, and eucalyptus Eucalyptus globulus oil and their combinations with two organophosphorus pesticides, Dimethoate 30 % and Pestban 48% were evaluated for the control of some Piercing-Sucking insect pests associated with Faba bean Vicia faba in open field, during season 2011/2012. All of the four composites diluted to 3% before applying for four times in each spray. Samples were taken at the first, fifth, seventh and tenth days after application to determine the reduction in numbers. The results showed that, garlic oil was the best efficient in reducing the population of leafhoppers and planthoppers by a mean reduction percentage of 68.09%, followed by the two chemical insecticides Dimethoate 67.90 % and Pestban 64.02 %, and Eucalyptus oil was the least in this category as 43.27 %. However in controlling aphids, also garlic surpasses again by a mean overall reduction 90.96 %, followed by Pestban 89.44 %, while eucalyptus oil came in the third rank as 80.66 % before the dimethoate which achieved the least rank in controlling aphids as 76.14 %. Furthermore experiment was conducted also to evaluate the side effects of such composites on the population fluctuations of two insect predators rife in Fababean fields, true spiders and minute pirate bug (Orius sp.). Data showed that, in compared with control, the use of natural oils preserved partly the coexist of natural enemies than the use of chemical compounds in Faba bean fields.