GC-MS analysis was carried out in order to investigate the phytoconstituents present in the ethanolic extract of Solanum americana leaves. The structural formula of the phytochemicals of S. americana leaves were determined by analysing of the spectra of GC-MS using the reference standard of National Institute of Standard and Technology (NIST). GC-MS analysis of S. americana leaves showed the presence of eighteen bioactive compounds. The compounds are 4-(5-(2-Naphthoxy)pentylthio)-5-chloro-uracil, propanamide, 2-(dimethylamino)-N-[2-methyl-1-[[3,3a,12,13,14,15,16,16a-octahydro-8-methoxy-13,16-dioxo-14, 4-Amino-1-methyl-5-nitropyrazole, 1,2-Benzenediol, o-octanoyl-o'-(thiophen-2-acetyl), 7H-1,4-Dioxino[2,3-c]xanthen-7-one, 2,3-dihydro-3-(4-hydroxy-3-methoxyphenyl)-2-(hydroxymethyl)-5-methoxy, benzoic acid, p-(cyclohexyloxy)-, 3-(2-methylpiperidino)propyl ester, quinoline, 6,6'-[2,6-naphthalenediyldi-2,1-ethenediyl]bis[1-ethyl-1,2,3,4-tetrahydro, 2,5,8,9,11-Pentaacetoxy-2,3-dihydrospiro(4H)benzofuro[2,3-g]-1-benzopyran-4,2'(5'H)-furan]-5'-one, 4-(d-Xylosyloxy)-6,7-dimethoxy-9-[3,4-(methylenedioxy)phenyl]naphtho[2,3-c]furan-1(3H)-one, benzenamine, N,N-didodecyl-4-nitro, 1-Hydroxy-3-(hydroxymethyl)anthraquinone, O,O'-bis(trimethylsilyl), d-Glucopyranoside, 1,4a,5,7a-tetrahydro-7-(methoxymethyl)cyclopenta[c]pyran-1,5-diyl[bis-, 2,2',3,3',4,4',6,6', 5,12-Naphthacenedione, 6,8,10,11-tetrakis(acetyloxy)-8-[(acetyloxy)acetyl]-7,8,9,10-tetrahydro-1-methoxy, salicylic acid, monoanhydride with 1-butaneboronic acid, cyclic ester, tetrabenzo[e,i,o,s][1,4,7,11,14,18]dithiatetraazacycloeicosine, 11,12,13,14,26,27-hexahydro, 6-Chloro-2-(3,5-diphenyl-pyrazol-2-yl)-benzothiazole, (23S,24S,25S)-26,26-dichloro-23-ethyl-6_-methoxy-3_,5:24,26-dicyclocholestane, 9H-Carbazole-9-carboxaldehyde, 1-hydroxy-3-methyl-2-(3-methyl-2-butenyl) and 2,4-cyclopentadiene-1,2,3,4-tetracarboxylic acid, 5-(4-bromo-2-quinolinyl)-, tetramethyl ester. It was concluded that the phytocompounds support the use of S. americana leaves for the management of pancreatic cancer, prostate cancer, asthma, allergic rhinitis, and osteoarthritis