Human Allergy Research Articles

Magnetic nanocomposite of filamentous algae activated carbon for efficient elimination of cephalexin from aqueous media

Discharge of antibiotics into the environment can cause problems like increase of the microorganisms’ resistance, disturbing the ecological balance and increasing the allergy in humans. In this research, an activated carbon was produced from filamentous algae and then magnetized with Fe3O4. The adsorbent size was nano-scale and its characteristics were studied using XRD, FT-IR, FE-SEM, BET and VSM techniques. The response surface method (RSM) was employed to optimize the operating parameters and determine the best conditions for cephalexin removal by novel composite of AC-Fe3O4. The various parameters in the process, such as reaction time, initial pH, adsorbent dose, initial concentration of cephalexin and effect of cations and anions that could interfere in the adsorption of cephalexin were evaluated in three levels. The proposed quadratic model was found to be best suggested model for the adsorption process (R2=0.99094 and $${\rm{R}}_{adj}^2=0.9991$$). According to results, the parameters such as cephalexin concentration, the adsorbent dose, the reaction time and the pH value were found to be 28.16 mg/L, 2 g/L, 30.04 and 3.02, respectively. Experimental results showed that the adsorption of cephalexin followed Langmuir isotherm (R2=0.9803). Also, the results showed cephalexin adsorption on the composite fitted pseudo-second-order kinetics. The study showed that the AC-Fe3O4 adsorbent has high efficacy for eliminating cephalexin from aqueous solution.

Infiltration: The disease of the structure

In civil construction, moisture has been highlighted as the main cause of infiltration, with the solution of waterproofing systems that aim to protect the environment. About this issue, this article aims to analyze and elucidate the infiltration process and it is consequences. In order to respond to the research objectives, the methodological choice was an exploratory and descriptive bibliographic review on the forms of pathological manifestation the buildings can present when affected by humidity. The research results show that the poor execution of the project and the lack of waterproofing in the structure are the main causes of structural pathologies. Found that the problem, the repair must be immediate. In addition, the study discusses the techniques and elements that should be used to avoid corrective repairs, preventing financial disorders, structural problems, public health and worsening of respiratory allergies in humans.

In vitro assessment of human seminal fluid IgE-mediated allergy

Human seminal plasma allergy is a rare clinical entity with symptoms varying from local reactions to life-threatening anaphylaxis after unprotected sexual intercourse. It can be associated with localized urticaria to skin contact and can be more severe as a result of condomless receptive anal sex. The in vitro investigation of specific serum IgE against human seminal fluid and molecular canine allergen Can f 5, cross-reactive with human prostate specific antigen (PSA), may be performed in clinical practice by solid-phase immunoassay methods such as singleplex fluoroimmunoassay and a new generation multiplex macroarray nanotechnology-based immunoassay.

Tissue-Resident Memory T Cells: Sheltering-in-Place for Host Defense

A silent revolution has occurred in our understanding of how T cell-mediated immunity protects the host from recrudescent pathogens and how it fits into occurrences of autoimmunity and allergies. Under the new paradigm, the hitherto unknown noncirculatory, tissue-resident memory T cells (TRM ) constitute the host defense sentinels posted in diverse anatomic compartments and they are the key actors in protection against reinfections, tissue surveillance, cancer, and in many cases in autoimmunity and allergy in both animal models and humans. This contrasts with the previously held view that circulating memory T cells (TcircM) transitioning through the peripheral tissue are the main defenders against reinfections and are underlying agents in autoimmune reactions. TRM, elicited after primary pathogen encounter in a given tissue, are now known to be stably positioned in the respective barrier (skin, lungs, gut, female reproductive tract mucosa, liver, etc.) or nonbarrier (brain, kidneys, etc.) peripheral tissues. TRM represent a rapid, tissue-autonomous, first line of robust adaptive immune defense against recurring infections. Following a discussion on the defining characteristics of TRM, this review will focus on how TRM seeding and induction at the site of recurrent pathogen invasion is now, and must continue to be, the governing principle in new vaccine designs. The review will also elaborate on the role of TRM in relapsing and remitting autoimmunity by being prepositioned in the tissue as potent effectors. Many infectious disease vaccines targeted to establish and activate TRM at the infection site in animal models are robustly more effective at host protection relative to their traditional, parenterally administered counterparts that only activate systemic TcircM. Likewise, TRM-centered remedies are being successful in ameliorating T cell mediated autoimmunity in cases in which approaches based on circulatory T cells failed. Thus, the current and emerging TRM discoveries are piloting a new era of TRM-driven strategies focused on activation or inactivation of tissue-localized immunity in vaccines and therapies targeting infectious disease, cancer, autoimmunity, and allergies.

Determining Effects of Winter Weather Conditions on Adult Amblyomma americanum (Acari: Ixodidae) Survival in Connecticut and Maine, USA.

The lone star tick (Amblyomma americanum L.) is native to the United States, with its primary range encompassing the Southeast and portions of the Midwest. It is an aggressive ectoparasite that actively seeks out hosts through detection of carbon dioxide and vibrations and can transfer ehrlichiosis-causing bacteria as well as a carbohydrate that causes alpha-gal syndrome (red meat allergy) in humans. It has become of increasing concern as its range has recently expanded into coastal regions of the Northeast. Historically, harsh northeastern winter weather conditions made these areas inhospitable for A. americanum survival, but a warming climate coupled with increased host availability seem to have facilitated their range expansion. We developed a study to observe the effects of weather conditions on adult A. americanum overwintering survival. The study was conducted over three years in Connecticut and Maine. Ground-level conditions were manipulated to determine the effects of differing combinations of natural insulative barriers (leaf litter and snow accumulation) on adult A. americanum survival. We determined that there was a significant difference in survival between the two states, between years in Maine, and between sexes within Connecticut. However, presence or absence of snow and/or leaf litter had no impact on survival. Overall, we found a positive correlation between mean hourly temperature and adult survival in Maine, where temperatures were consistently below freezing. The results of this study can be included in an adaptive, predictive analytic model to accommodate the expected fluctuations and range expansion of A. americanum that will most likely accompany an increase in temperatures throughout the Northeast.

In vitro selection of DNA aptamers and their integration in a competitive voltammetric biosensor for azlocillin determination in waste water

The uncontrolled usage of veterinary antibiotics has led to their widespread pollution in waterways and milk products. Potential impact of antibiotic residues on the environment and human health such as increased antibiotic resistance of microorganisms and triggering allergic reactions in humans have been reported. In this work, we developed a highly selective and sensitive voltammetric aptasensor for on-step, sensitive and low cost detection of azlocillin antibiotic, one of the broad spectrum β-lactam antibiotics. The successful selection of DNA aptamers against azlocillin was accomplished using systemic evolution of ligands by exponential enrichment (SELEX) method. Fluorescence-binding assays showed dissociation constant of 55 nM for one of the selected aptamers (Az9). This aptamer was used to construct a competitive voltammetric aptasensor for azlocillin. A limit of detection of 1.2 pg/mL as well as remarkable selectivity against potential interfering agents, including amoxicillin, were achieved. This signal-off competitive sensor takes 30–50 min to complete the quantification of the target antibiotic. The sensor was challenged by detecting the target directly in complex environments such as tap and waste water where good recovery percentages were achieved.

Gap analysis of nickel bioaccessibility and bioavailability in different food matrices and its impact on the nickel exposure assessment.

The metal nickel is well known to cause nickel allergy in sensitive humans by prolonged dermal contact to materials releasing (high) amounts of nickel. Oral nickel exposure via water and food intake is of potential concern. Nickel is essential to plants and animals and can be naturally found in food products or contamination may occur across the agro-food chain. This gap analysis is an evaluation of nickel as a potential food safety hazard causing a risk for human health. In the first step, the available data regarding the occurrence of nickel and its contamination in food and drinks have been collected through literature review. Subsequently, a discussion is held on the potential risks associated with this contamination. Elevated nickel concentrations were mostly found in plant-based foods, e.g. legumes and nuts in which nickel of natural origin is expected. However, it was observed that dedicated and systematic screening of foodstuffs for the presence of nickel is currently still lacking. In a next step, published studies on exposure of humans to nickel via foods and drinks were critically evaluated. Not including bioaccessibility and/or bioavailability of the metal may lead to an overestimation of the exposure of the body to nickel via food and drinks. This overestimation may be problematic when the measured nickel level in foods is high and bioaccessibility and/or bioavailability of nickel in these products is low. Therefore, this paper analyzes the outcomes of the existing dietary intake and bioaccessibility/bioavailability studies conducted for nickel. Besides, the available gaps in nickel bioaccessibility and/or bioavailability studies have been clarified in this paper. The reported bioaccessibility and bioavailability percentages for different food and drinks were found to vary between <LOD and 83% and between 0 and 30% respectively. This indicates that of the total nickel contained in the foodstuffs only a fraction can be absorbed by the intestinal epithelium cells. This paper provides a unique critical overview on nickel in the human diet starting from factors affecting its occurrence in food until its absorption by the body.

Chronic infection with the protozoan Toxoplasma gondii prevents the development of experimental atopic dermatitis in mice

BackgroundSupporting the hypothesis thatT. gondii infection protects against allergy in humans we previously demonstrated that this infection can modulate not only the susceptibility to develop respiratory allergies in mice but also suppresses allergic responses at systemic level. This latter finding suggests that T. gondii infection could prevent the onset of other allergic diseases, such as atopic dermatitis. At present, few studies have investigated the modulation of atopic dermatitis by parasite infections. ObjectiveHere, we sought to investigate whether chronic infection with T. gondii is capable of modulating the development of atopic dermatitis. MethodsChronically infected mice were sensitized by repeated epicutaneous ovalbumin administration. Skin histopathology, humoral response, cytokine production and innate type-II lymphoid cells (ILC2) were assessed. ResultsA marked reduction in epidermal thickness and dermal inflammatory infiltrate along with a reduction in mast cell count was observed in infected mice compared to non-infected mice. These results correlated with a diminished TH2 and TH1 allergen specific response. Reduced type-II IL-4 and IL-5 cytokines were already detected during the first 24 h of allergen sensitization in splenocytes and draining lymph nodes from infected mice. Moreover, this reduced type-II profile in chronically infected animals correlated with diminished ILC2 number in draining lymph nodes. ConclusionChronic infection withT. gondii prevents the development of atopic dermatitis. The diminished susceptibility seems to result from changes in type-II innate immune response that may lead to the induction of a deficient TH2 response and consequently to a lower susceptibility to develop atopic dermatitis.

Variation in Morphology and Airborne Dispersal of the Urticating Apparatus of Ochrogaster lunifer (Lepidoptera: Notodontidae), an Australian Processionary Caterpillar, and Implications for Livestock and Humans.

True setae borne on the abdominal tergites of Ochrogaster lunifer Herrich-Schӓffer caterpillars are the agents of an irritating contact dermatitis, osteomyelitis, ophthalmia, and severe allergic reactions in humans, and are the cause of Equine Amnionitis and Fetal Loss in Australia. The setae are detached and readily dislodge from the integument whereby they disperse throughout the environment. To better understand the true setae of O. lunifer as agents of medical and veterinary concern, we studied their characteristics and distance dispersed. Whereas members of the European Thaumetopoeinae have been widely studied, their southern-hemisphere counterparts such as O. lunifer are not well known despite their harmfulness and known medical and veterinary importance. The caterpillar’s investment in true setae increased with age and size, and two distinct size classes co-occurred in setae fields. A previously undescribed morphological type of true seta was found on the first abdominal segment. All true setae were calculated to travel long distances in the air even under light breeze conditions. Our results show there is a high risk of exposure to airborne urticating setae within 100 m of elevated caterpillar activity, and a likely risk of exposure for some kilometers in the direction of the prevailing breeze. This information should be used to inform management strategies in areas where urticating processionary caterpillars are active, and especially during periods of an outbreak.

Cinnamon extract inhibits allergen-specific immune responses in human and murine allergy models.

Ceylon cinnamon has been shown to possess anti-inflammatory properties in many diseases including allergic inflammation. The aim of this study was to analyse in more detail the effects of cinnamon extract (CE) and its major compounds p-cymene and trans-cinnamaldehyde (CA) on allergen-specific immune responses in vitro and in vivo. Therefore, monocyte-derived mature dendritic cells (DC) from grass or birch pollen allergic donors were pulsed with the respective allergen in the presence or absence of CE, p-cymene, CA or the solvent ethanol and co-cultured with autologous CD4+ T cells. Furthermore, basophil activation test was performed with or without CE or ethanol treatment. For the in vivo experiments, BALB/c mice were immunized with ovalbumin (OVA) and orally treated with CE or ethanol. Addition of CE, p-cymene or CA, but not ethanol significantly inhibited DC maturation and subsequent allergen-specific T cell proliferation as well as Th1 and Th2 cytokine production. Sulphidoleukotriene release and CD63 expression by basophils were also significantly diminished after addition of CE. In vivo, treatment of OVA-sensitized mice with CE led to a significant shift from OVA-specific IgE towards IgG2a production and to a strong inhibition of OVA-specific proliferation. Moreover, airway inflammation as well as anaphylaxis after intranasal or systemic allergen challenge was significantly reduced in CE-treated mice. Furthermore, topical application of CE prevented calcipotriol-induced atopic dermatitis-like inflammation in these mice. Taken together, our data indicate that the anti-inflammatory effect of cinnamon might be exploited for treatment of allergic inflammation, which needs to be further investigated.

Climate change and plant pathogen biodiversity: The case of witches’ broom outbreak on Vachellia xanthophloea (Benth.) Banfi &amp; Galasso) in Botswana

Climate change, typified by drought, floods and rising temperatures continue to affect biodiversity in the world. Recently, outbreaks of new insect, weed and plant pathogen species have been reported in SADC and some old ones have increased in severity while others have diminished. Examples of new insects that have threated food security and trade in the region include the fall army worm (Spodoptera frugiperda) and tomato leaf miner (Tuta absoluta). Over 50% of the Hartbeespoort Dam near Pretoria is covered by water hyacinth (Eichhornia crassipes) reducing property values and tourism in the area. In August 2014, witches’ broom outbreak on fever tree was first observed in Gaborone. Microscopic examination of disease signs revealed, urediospores, teliospores, aeciospores and aecia typical of Ravenelia sp. Samples were sent to the FABI, University of Pretoria where the pathogen was tentatively identified as R. natalensis or R. hieronymi. After the 2014/2015 and 2015/16 poor rain seasons few new infections were recorded in August. Following the heavy rains caused by cyclone Dineo, the epiphytotic exploded starting July 2018 and almost all fever trees in the major towns of Botswana were attacked. The witches’ brooms were also infected by powdery mildew (Erysiphe sp.) and Diplodia sp. which caused black twig dieback. The fever tree natural distribution is limited to the Tuli block but it has been introduced south as a landscape and shade tree. The tree is now invasive spreading rapidly along drainage channels and rivers around Gaborone. Currently the pathogen may be a threat to other Vachellia and Senegalia spp. in Botswana. The witches’ brooms are releasing trillions of spores into the air which may result in new human allergies. The implications of the outbreak on tree biodiversity and how the disease can be managed should be investigated.

New Data on the Range Expansion of the Thaumetopoea pityocampa (Lepidoptera: Notodontidae) 'ENA clade' in Greece: The Role of Bacterial Endosymbionts.

The pine processionary moth, Thaumetopoea pityocampa (Denis and Schiffermüller), is an important insect in the Mediterranean region, as it defoliates pines and its urticating hairs can cause allergic reactions in humans and animals. Moreover, this species exhibits an interesting genetic structure as recently a distinct East-North African mtDNA lineage ('ENA clade') has been described. This clade has been recently detected in Greek populations where it has currently expanded its range by replacing the 'endemic' T. pityocampa lineages. Here, we report new data on the rapid spread of 'ENA clade' in the Greek island Evoia in only a few years. As the underlying mechanisms of the 'ENA clade' range expansion has not been studied so far, we screened T. pityocampa for an infection with the heritable bacterial endosymbionts Wolbachia (Bacteria: Anaplasmataceae), Cardinium (Bacteria: Bacteroidaceae), Rickettsia (Bacteria: Rickettsiaceae) and Spiroplasma (Bacteria: Spiroplasmataceae). These bacteria can manipulate the reproduction of infected hosts, something that could potentially explain the rapid spread of 'ENA clade' lineage. Therefore, we screened 28 individuals that exhibited T. pityocampa 'ENA clade' and 'endemic' T. pityocampa haplotypes from nine populations scattered all over Greece. None of them was infected with any of the four endosymbionts, suggesting that these bacteria do not cause reproductive manipulations in T. pityocampa lineages and, thus, other factors should be explored in future research efforts.

Genome-wide analysis of Anisakis simplex sensu lato: the role of carbohydrate metabolism genes in the parasite’s development

Anisakis simplex sensu lato is a parasitic nematode which can cause gastric symptoms and/or allergic reactions in humans who consume raw and undercooked fish. Anisakiasis poses a growing health problem around the globe because it causes non-specific symptoms and is difficult to diagnose. This genome-wide study was undertaken to expand our knowledge of A. simplex s.l. at the molecular level and provide novel data for biological and biotechnological research into the analyzed species and related nematodes. A draft genome assembly of the L3 stage of A. simplex s.l. was analyzed in detail, and changes in the expression of carbohydrate metabolism genes during the parasite's life cycle were determined. To our knowledge, this is the first genome to be described for a parasitic nematode of the family Anisakidae to date. We identified genes involved in parasite-specific pathways, including carbohydrates metabolism, apoptosis and chemo signaling. A total of 7607 coding genes were predicted. The genome of A. simplex s.l. is highly similar to genomes of other parasitic nematodes. In particular, we described a valuable repository of genes encoding proteins of trehalose and glycogen metabolism, and we developed the most comprehensive data set relating to the conversion of both saccharides which play important roles during the parasite's life cycle in a host environment. We also confirmed that trehalose is synthesized at the expense of glycogen. Trehalose anabolism and glycogen catabolism were the predominant processes in stages L4 and L5, which could confirm our and other authors’ previous reports that trehalose is synthesized at the expense of glycogen. The A. simplex s.l. genome provides essential data for post-genomic research into the biology of gastrointestinal and allergic anisakiasis in humans and the biology of other important parasitic helminths.

Modulating Shrimp Tropomyosin-Mediated Allergy: Hypoallergen DNA Vaccines Induce Regulatory T Cells to Reduce Hypersensitivity in Mouse Model.

Shellfish allergy is one of the most common food allergies, with tropomyosin as the major cross-reactive allergen. However, no allergen-specific immunotherapy is clinically available. Recently, we designed two shrimp hypoallergens MEM49 and MED171. This study aimed to examine and compare the efficacy of the MEM49- and MED171-based DNA vaccines (pMEM49 and pMED171) in modulating shrimp allergy in a murine model of shrimp tropomyosin sensitivity. Intradermal immunization of BALB/c mice with pMEM49 or pMED171 effectively down-modulated allergic symptoms, tropomyosin-specific IgE levels, intestinal Th2 cytokines expression, and inflammatory cell infiltration. Both pMEM49 and pMED171 increased the frequency of regulatory T cells, but to a greater extent by pMED171 with upregulation of gut-homing molecules integrin-α4β7. The functionality of the pMED171-induced Treg cells was further illustrated by anti-CD25-mediated depletion of Treg cells and the adoptive transfer of CD4+CD25+Foxp3+Treg cells. Collectively, the data demonstrate that intradermal administration of pMED171 leads to the priming, activation, and migration of dermal dendritic cells which subsequently induce Treg cells, both locally and systemically, to downregulate the allergic responses to tropomyosin. This study is the first to demonstrate the potency of hypoallergen-encoding DNA vaccines as a therapeutic strategy for human shellfish allergy via the vigorous induction of functional Treg cells.

Reviews of Science for Science Librarians: Alpha-Gal: The Curious Case of Ticks Whose Bite Induces Red Meat Allergies in Humans

ABSTRACTA growing number of tick species have now been demonstrated to induce red meat allergies through biting humans. During the course of the bite, Galactose alpha-1,3-Galactose, commonly abbreviated as alpha-Gal, a carbohydrate not found in humans, but common in four-legged mammals, makes its way through to the bloodstream, triggering antibody production. Subsequent consumption of red meat by bite victims sets off a peculiarly delayed immunological reaction that may range from hives through to anaphylaxis. This paper documents the discovery of its linkage with tick bites. The spread of this syndrome worldwide warrants the attention of scientific and clinical librarians serving allergists, immunologists, dieticians, and entomologists.

Study on the Optimization of Process Parameters for Absorbable Bone Plate with In-Mold Heat Treatment

Titanium alloy &amp; stainless steel are major materials for bone fracture fixation such as bone screws and plates in today's medical devices. However, the fixation devices made of metal not only have the risk of metal ion release to cause human allergies, but they also need to be removed by a second surgical operation making pain and the risk of the patient’s wound infection after the bone fracture healing. The biodegradable Polylactic Acid (PLA) bone screws &amp; bone plates have the great advantage of not needing a second operation, but their insufficient strengths make them not be widely used in the current bone fracture fixation. In the study, we use PLA as the matrix and in-mod heat treatment with induction coils to increase the strength of bone screw &amp; plate by improving crystallinity of material. Regarding ASTM F2502 “Standard Specification and Test Methods for Absorbable Plates and Screws for Internal Fixation Implants”, we measure bending loads of test pieces before and after in-mold heat treatment, and obtain the optimized process parameters by Taguchi method that will increase the bend load of PLA bone plates by 34.82%. These optimal parameters are the injection speed of 80 mm/s, the melt temperature of 205 °C, the heat treatment temperature of 110 °C and heat treatment time of 20 min.

IgG from Non-atopic Individuals Induces In Vitro IFN-γ and IL-10 Production by Human Intra-thymic γδT Cells: A Comparison with Atopic IgG and IVIg

Matured in the thymus, γδT cells can modulate the development of allergy in humans. The main γδT cell subsets have been described as interleukin (IL)-17A or interferon (IFN)-γ producers, but these cells can also produce other modulatory cytokines, such as IL-4 and IL-10. Here, we aimed to evaluate whether IgG can modulate the profile of cytokine production by γδT cells during their maturation in the thymus and after its migration to peripheral tissues. Thymic tissues were obtained from 12 infants, and peripheral blood mononuclear cells (PBMCs) were obtained from adults (both groups without an atopic background). IgG was purified from atopic and non-atopic volunteers. Thymocytes and PBMCs were cultured with purified atopic or non-atopic IgG, and intracellular cytokine production and phenotype were assessed. Mock and IVIg conditions were used as controls. IgG from non-atopic individuals induced IFN-γ and IL-10 production by thymic γδT cells, and no effect was observed on peripheral γδT cells. IL-17 production was inhibited by non-atopic IgG on thymic γδT cells and augmented by atopic IgG on peripheral γδT cells. Modulated thymic γδT cells did not produce IFN-γ and IL-10 simultaneously. We additionally evaluated the phenotype of intrathymic γδT cells and observed that IgG from all groups could induce CD25 expression and could not influence the CD28 expression of these cells. This report describes evidence revealing that IgG may influence the production of IFN-γ and IL-10 by intrathymic γδT cells depending on the donor atopic state. This observation is unprecedented and needs to be considered in further studies in the IgG immunotherapy field.

Proteome profiling of L3 and L4 Anisakis simplex development stages by TMT-based quantitative proteomics

Anisakis simplex is a parasitic nematode that can cause anisakiosis and/or allergic reactions in humans. The presence of invasive third-stage larvae (L3) in many different consumed fish species and the fourth-stage larvae (L4) in marine mammals, where L3 can accidentally affect to humans and develop as far as stage L4. World Health Organization and food safety authorities aim to control and prevent this emerging health problem. In the present work, using Tandem Mass Tag (TMT)-based quantitative proteomics we analyzed for the first time the global proteome of two A. simplex development stages, L3 and L4. The strategy was divided into four steps: (a) protein extraction of L3 and L4 development stages, (b) high intensity focused ultrasound (HIFU)-assisted trypsin digestion, (c) TMT-isobaric mass tag labeling following by high-pH reversed-phase fractionation, and (d) LC-MS/MS analysis in a LTQ-Orbitrap Elite mass spectrometer. A total of 2443 different proteins of A. simplex were identified. Analysis of the modulated proteins provided the specific proteomic signature of L3 (i.e. pseudocoelomic globin, endochitinase 1, paramyosin) and L4 (i.e. neprilysin-2, glutamate dehydrogenase, aminopeptidase N). To our knowledge, this is the most comprehensive dataset of proteins of A. simplex for two development stages (L3 and L4) identified to date. SignificanceA. simplex is a fish-borne parasite responsible for the human anisakiosis and allergic reactions around the world. The work describes for the first-time the comparison of the proteome of two A. simplex stages (L3 and L4). The strategy is based on four steps: (i) protein extraction, (ii) ultra-fast trypsin digestion under High-Intensity Focused Ultrasound (HIFU), (iii) TMT-isobaric mass tag labeling followed by high-pH reversed-phase fractionation and (iv) peptide analysis using a LTQ-Orbitrap Elite mass spectrometer. The workflow allows to select the most modulated proteins as proteomic signature of those specific development stages (L3 and L4) of A. simplex. Obtained stage-specific proteins, could be used as targets to control/eliminate this parasite and in future eradicate the anisakiosis disease.

Consumer protection and risk assessment: sensitising substances in consumer products

The human immune system is confronted daily with a large, chemically-varied range of potentially sensitising substances. Skin sensitising substances are found, above all, in a plethora of consumer products, e. g. cosmetics, jewellery, earrings, toys, textiles, leather, other everyday commodities and, in some cases, also tattoos. These products may contain sensitisers such as fragrances, preservatives, dyes, or other additives. To provide a greater degree of consumer protection, there is a need for specific legal regulation and risk assessment, which covers each possible human exposure to a sensitising substance or mixture. This review article describes the background and pathway towards the development and implementation of an international legal framework for the classification and labelling of chemicals that contain potentially skin sensitising substances. This includes the implementation of the globally harmonized system of classification and labelling of chemicals (GHS), the classification, labelling and packaging (CLP) regulation, registration, evaluation, authorisation and restriction of chemicals (REACH), and the regulation of cosmetics, among other national laws and regulations. Assessment criteria for classification is derived from a suite of in vitro and in vivo assays, in addition to in silico approaches—validated by the organisation for economic cooperation and development (OECD)—as well as data derived from human studies. New legislation for chemical and product safety is reflected in the classification and labelling of skin sensitising substances under Category 1, Subcategory 1A or 1B, within which the threshold concentrations of several materials are regulated, e. g. p‑phenylenediamine in hair dyes, nickel in piercings, chromium VI in leather and methylisothizolinone in cosmetics. In order to minimise the risk of human contact allergy from consumer products, the scientific committee on consumer safety (SCCS) and the German federal institute for risk assessment (BfR) investigate pathways of exposure and perform risk assessments using new in vitro approaches and new (immuno-) toxicological concepts (i. e. adverse outcome pathways [AOPs], key events as well as an integrated approach to testing and assessment [IATA]). In comparison to cosmetics, substances in textiles and other consumer products are less regulated. Major efforts in research and development are necessary to decode complex substance-specific molecular mechanisms in allergic responses and to define new substance-specific thresholds. Such efforts have been continuously proposed by the BfR with regard to fragrances for over 10 years. Today, skin sensitising substances can be legally regulated and labelled and, depending on the exposure, their content in consumer products can be reduced or eliminated. Furthermore, the risk assessment of potentially sensitising substances makes consumer products safer. Further improvements in research approaches are required in the area of health and consumer protection with regard to allergy.

STRUCTURE OF SOIL COMPLEXES OF MICROSCOPIC FUNGI UNDER DIFFERENT VARIETIES OF BROAD BEANS

Considering of broad beans as an integral component of biological crop rotations, the presence of phytopathogenic, toxigenic, opportunistic, and allergenic microscopic fungi in soils under their different varieties should be examined. At the Botanical garden BelSU (Belgorod) on a natural infectious background were grown and studied of 16 different broad beans varieties. Were taking into account the indicators of frequency of occurrence and abundance of species, they determined the structures of soil mycocomplexes under different varieties. Analysis of the data showed that most micromycetes in the rhizosphere under bean varieties belonged to the Ascomycota division, which is part of it as 4th classes of Sordariomycetes, Dothideomycetes, Eurotiomycetes and Saccharomycetes. The species diversity of the soil mycobiota of steaming soil was characterized by 26 species, and under the studied bean varieties 57 species of fungi were identified. In the mycocomplex under varieties, species of different ranks of frequency of occurrence developed that were not noted in the paring soil: Alternaria tenuissima and Stemphyllium solani were rarely and accidentally found in the control soil, but they were not found in under the beans during budding – flowering. This can be explained by the fact that a different composition of exudates in the root zone of the soil under the plants contributes to the development of different types of microorganisms. In the complex of micromycetes under the beans, the share of toxigenic species increased by 2.5-29%, opportunistic species - by 1.3-31%, allergens - by 2-24% compared with the soaring soil. In the soil, an abundance of opportunistic and allergenic species capable of inducing mycoses and allergies in humans can be reduced by the broad bean varieties Aquadul, White large-fruited, Russian Black, Velena, Summer Resident, Leader, Optics, Pink Flamingo, Yankel Byala. After the cultivation of the beans the phytopathogenic species Ascochyta fabae, Clasdosporium herbarum, Fusarium graminearum, F. oxysporum var. orthoceras, F. oxysporum, F. solani and Ulocladium botrytis, remain in the soil and this fact must be considered when selecting the next crop in crop rotation.