Four compounds from each of the sodium alkyl sulphate, 3-alkyl-7-hydroxy cou marin sulphonate, and alkaryl-2-pyrazoline sulphonate series of anionic surfactants were applied to wool under conditions such that equilibrium distribution would occur throughout the fibers. The treated wools were then extracted under various conditions of time, pH, and temperature, and also with various surfactants or alcohols in the extraction solutions. Aqueous extractions, even at pH 10, were relatively ineffective in removing the more surface active members of each series. Solutions containing alcohols, especially isopropanol/pH 10 buffer (1:1), were particularly effective in ex tracting the surfactants from wool. The substantivities to wool of fluorescent anionic surfactants containing coumarin or pyrazoline fluorophores are similar to those of the sodium alkyl sulphate surfactants of similar critical micelle concentration.