Alkaline Phosphatase Activity In Response Research Articles

Observations on the Alkaline Phosphatase Activity of Leucocytes in Adrenalectomised Rats

The alkaline phosphatase activity of circulating polymorphonuclear leucocytes was investigated in rats, using a semi-quantitative cytochcmical method. In normal rats there was a rise in leucocyte alkaline phosphatase activity in response to adrcnocorticotrophic hormone; a much greater rise occurred when rats were injected subcutancously with turpentine. The positive response to ACTH was abolished by adrenalectomy, but in adrenalectomised rats the increase in leucocyte alkaline phosphatase activity associated with the turpentine-induced lesion was undiminished. These results imply that the alkaline phosphatase activity of leucocytes can be modified by at least two agents, one of which is probably an adrenocortical hormone, the other an unidentified factor, not of adrenal origin, but associated with tissue injury.

Bullous Eruption and Elevated Leukocyte Alkaline Phosphatase in the Course of Busulfan-Treated Chronic Granulocytic Leukemia

Abstract A 35-year-old man presented with a bullous eruption similar to erythema multiforme following the administration of high doses of busulfan therapy for chronic granulocytic leukemia. There was pancytopenia, hypoplasia of the bone marrow, elevation of serum vitamin B12 and B12 binding capacity and a paradoxical elevation of the leukocyte alkaline phosphatase level. Discontinuation of the busulfan and the administration of prednisone were followed by gradual healing of the skin lesions, reversal of the pancytopenia and reduction of the leukocyte alkaline phosphatase activity to zero. This case illustrates an unusual and previously unreported untoward response to busulfan and is another example of the capacity of the leukocytes in some patients with chronic granulocytic leukemia to develop high alkaline phosphatase activity in response to inflammation.

Vascular perfusion of isolated rat gut: norepinephrine and glucocorticoid requirement.

Vascular perfusion of isolated rat gut: norepinephrine and glucocorticoid requirement.