Alkaline Hydrogen Peroxide Pretreatment Research Articles

Investigation of the alkaline hydrogen peroxide pretreatment: From cellulose saccharification to lignin isolation

Alkaline hydrogen peroxide (AHP) pretreatment, a green and effective technology for biomass fractionation, was studied to overcome the recalcitrance of bamboo for lignin isolation and facilitating enzymatic saccharification. In order to explore the impact of AHP pretreatment on the cellulose saccharification of bamboo, the glucan/xylan recovery, lignin removal, and structural properties of solid substrate were investigated in this research. AHP pretreatment at 90 ℃ for 60 min with 3.0 % H2O2 resulted in the maximum glucan/xylan hydrolysis yield reaching 90.62 % and 88.30 %, respectively. Specially, for the first time, the recovered lignin from the AHP liquid possesses up to 59.84/100Ar of β-O-4 linkages, abundant ferulic acid (FA), and p-coumarate (pCE) structures, as well as a homogeneous molecular structure with high purity (84.04%-96.20%) and preferable antioxidant activities (IC50: 0.015–0.018 mg/mL). These results provided theoretical support for the comprehensive valorization of lignocellulose, which is favorable for achieving viable biorefineries.

Recovery of p-Hydroxybenzoic Acid from Cu-Catalyzed Alkaline Hydrogen Peroxide Pretreatment of Hybrid Poplar

Recovery of <i>p</i>-Hydroxybenzoic Acid from Cu-Catalyzed Alkaline Hydrogen Peroxide Pretreatment of Hybrid Poplar

Efficacy of alkaline peroxide pretreatment on Eucalyptus grandis as effective lignin removal strategy for production of ethanol using enzymatic hydrolysis

Efficacy of alkaline peroxide pretreatment on Eucalyptus grandis as effective lignin removal strategy for production of ethanol using enzymatic hydrolysis

CHARACTERIZATION OF LIGNIN DISSOLVED DURING ALKALINE SODIUM BOROHYDRIDE AND HYDROGEN PEROXIDE PRETREATMENTS OF SUGAR MAPLE (ACER SACCHARUM)

Sugar maple (Acer saccharum) wood chips were pretreated with aqueous sodium hydroxide, sodium borohydride, and hydrogen peroxide pretreatments, and the characterization (i.e., amount, molar masses, and molar mass distributions) of the lignin dissolved during the pretreatments was performed. Of the applied pretreatments, especially borohydride pretreatments were efficient in dissolving lignin from this feedstock, as 20% to 28% of the dissolved organics were found to consist of dissolved lignin, contributing to 3.4% to 9.0% of the initial wood lignin. Corresponding values for 2.5% and 5.0% alkaline peroxide pretreatments were from 5.9% to 9.2% dissolved lignin of the total dissolved organics and from 1.1% to 3.2% of the initial wood lignin. Additionally, determined molar masses and molar mass distributions indicated that borohydride pretreatments generally led to the formation of smaller-molar-mass lignin fragments when compared to reference pretreatments and to pretreatments conducted with alkaline peroxide process, indicating more efficient lignin degradation caused by borohydride addition. For example, Mn and Mw values of the analyzed reference effluents were 54%-62% and 66%-71% higher when compared to the corresponding borohydride effluents, respectively.

Detoxification and enhancement of in vitro rumen digestibility of exhausted olive pomace wastes through alkaline hydrogen peroxide treatment

Abstract Background Due to the sharp rise in animal feed costs, funding alternatives to substitute high-cost raw materials used in animal feed is a persistent need. This study investigated the effect of alkaline hydrogen peroxide pretreatment as straightforward non-toxic technology to enhance the in vitro rumen digestibility of exhausted olive pomace (EOP), an abundant agricultural waste, to be suitable as animal feedstock. It examined the efficiency to eliminate the toxic phenolic content and minimize lipid oxidation of EOP. Results The pretreatment was first optimized using a central composite experimental design. Under the optimized conditions (1.6% H2O2, 5% NaOH), the measured phenolic content was 1.51 ± 0.03 mg/100 g dry weight (DW) for treated olive pomace (TOP) versus 4.91 ± 0.06 mg/100 g for the untreated one. The pretreatment showed that approximately 25% of the lignin was removed. Crude proteins, neutral detergent fibers, and acid detergent fibers yields of TOP were, respectively, 3.320 ± 0.05, 75.24 ± 0.23, and 54.05 ± 0.35 g/100 g of DW, significantly more important than those of untreated EOP. The enzymatic hydrolysis with a cellulase-based cocktail (Celluclast15 FPU/gDW), recorded a 48% of reducing sugar yield for TOP against 33% for EOP. When the in vitro organic matter digestibility (IVOMD) was assayed, the potential gas production of TOP (41.371 ml/g DM) was significantly higher than EOP (25.771 ml/g DM). The metabolizable energy of TOP (9.28 kcal/kg DM) was higher than that of EOP (7.78 kcal/kg DM). Conclusions The present study revealed that alkaline hydrogen peroxide (AHP) could be an efficient treatment for the detoxification and enhancement of in vitro rumen digestibility of olive pomace. This straightforward approach demonstrated that treated olive pomace waste may be well valorized as suitable animal feedstock. Graphical Abstract

Anaerobic fermentation of poplar processing residue to produce methane by alkaline hydrogen peroxide (AHP) pretreatment

ABSTRACT The study evaluated the effects of alkaline hydrogen peroxide (AHP) pretreatment on poplar processing residue for methane production. The delignification of poplar processing residue was used as the basis for evaluation. The experiment considered the pretreatment time (0 h-24 h), the hydrogen peroxide (H2O2) concentration (0.5%-2.0%), and potassium hydroxide (KOH) concentration (1.0%-3.0%) at a temperature of 70°C. Subsequently, anaerobic fermentation experiments were conducted using the pretreated biomass with sludge as the inoculum under mesophilic temperature conditions (37 ± 1°C). The initial feedstock (poplar processing residue) contained 40.31 ± 0.06% cellulose, 31.17 ± 1.59% lignin, and 14.85 ± 0.38% hemicellulose. The AHP pretreatment caused changes in the lignocellulosic content of the feedstock, particularly affecting the lignin structure compared to cellulose and hemicellulose. At a pretreatment time of 18 hours, temperature of 70°C, and concentrations of 2.0% H2O2 and 1.0% KOH, the AHP pretreatment resulted in a higher methane production of 237.4 mL per gram. These findings demonstrate that AHP pretreatment is an effective strategy for enhancing methane production from poplar.

Effective cellulose isolation from torch ginger stem by alkaline hydrogen peroxide – Peracetic acid system

Efficient and eco-friendly isolation of cellulose from non-woody plants is paramount. This study aimed to isolate cellulose from torch ginger stem (Etlingera elatior) (TGS) waste efficiently by alkaline hydrogen peroxide pre-treatment and subsequently by peracetic acid treatment (AHP-PAA) under autoclave conditions (121 °C/0.1 MPa). A complete randomized design with three factors—AHP concentration (0, 16, and 33 % (v/v) H2O2 in 5 % (w/v) NaOH), PAA concentration (PAA to water ratio of 0.36, 1.14, and 4.00 (v/v)), and pre-treatment time (30, 60, and 90 min)—was used to investigate changes in yield and fiber’s chemical components. The results showed that TGS is a potential non-wood cellulose source with a high S/G ratio (3.016) and low SiO2 (4.2 %). Analysis of variance exhibited that the AHP and PAA concentration affected lignin-related parameters, but pre-treatment time influenced only carbohydrate-related parameters. The optimum isolation condition resulted in 47.78 % pulp yield with 64.78 % α-cellulose, 34.06 % hemicellulose, 0.15 % lignin, and 71.8 % crystallinity in 90 min of reaction time. Massive liberation of individual fibers from bundles, single peak on thermal degradation, removal of lignin-related functional groups and some metal oxide also supported the effectiveness of the AHP-PAA method to isolate TGS cellulose through prehydrolysis, chromophore group deactivation, and delignification reactions.

A waste-free biorefinery pathway to the valorisation of Chinese hickory shell through alkaline hydrogen peroxide pretreatment

Efficiently harnessing the intrinsic value of lignocellulose requires the integration of multifarious capacities within a zero-waste biorefinery. Chinese hickory shell, a food industry byproduct, remains largely untapped. Unleashing their full potential as a valuable biomass resource has become an urgent challenge. This study utilises the alkaline hydrogen peroxide pretreatment for Chinese hickory shell, exploring the effect of H2O2 dosage, alkali concentration, and temperature on lignocellulosic fractionation. Under optimal conditions, nearly complete removal of lignin and hemicelluloses is achieved, with cellulose enzymatic hydrolysis up to 98.1%, surpassing the original material by over 15-fold. Simultaneously, hemicelluloses transformed into high-value organic acids, yielding 33.5%. Notably, fractionated lignin is abundant in aryl ether linkages after AHP, facilitating subsequent transformation into aromatic chemicals. Through AHP, efficient fractionation of Chinese hickory shell is achieved, yielding fermentable glucose, organic acids, and lignin enriched with high aryl ether content. This research emphasises the pivotal role of comprehensive Chinese hickory shell utilisation in elevating the revenue of zero-waste biorefinery, sparking the production of valuable products from biomass waste streams, and further contributing to establishing resource-efficient biorefineries.

Optimization of Alkaline Hydrogen Peroxide Pretreatment and Enzymatic Hydrolysis of Wheat Straw for Enhancing Sugar Yields

Optimization of alkaline hydrogen peroxide (AHP) pretreatment and enzymatic hydrolysis of wheat straw (WS) was carried out to enhance fermentable sugar yields with the use of glucose and xylose yields from the pretreated WS as responses. In the first step, variables including temperature, hydrogen peroxide concentration and time during pretreatment were detected to have significant effects on the sugar yields. The results indicate that maximal sugar yields could be obtained while the WS was pretreated using 71 g/L hydrogen peroxide solution with 200 g/L of solid loading at 50 °C for 7.6 h. The corresponding cellulose recovery, hemicellulose recovery and lignin removal were 97.5%, 84.3% and 75.0%, respectively. In the second step, enzymatic hydrolysis of the pretreated WS was optimized. The results show that the reaction time, enzyme loading and biomass loading during enzymatic hydrolysis also had significant effects on the sugar yields. The final maximum yields of glucose (552.7 mg/gds (mg/g dry substrate)) and xylose (223.6 mg/gds) could be obtained while enzymatic hydrolysis was carried out at 50 °C for 37.0 h using 10.8 FPU/gds (filter paper activity unit per gram dry substrate) of enzyme loading, 88 g/L of biomass loading and 0.3% (w/v) of Tween-80. The corresponding cellulose conversion and hemicellulose conversion were 94.0% and 83.5%, respectively.

Insights into the capability of the lignocellulolytic enzymes of Penicilliumparvum 4-14 to saccharify corn bran after alkaline hydrogen peroxide pretreatment

BackgroundCorn bran is a major agro-industrial byproduct from corn starch processing. It contains abundant arabinoxylan that can be converted into value-added chemicals via biotechnology. Corn bran arabinoxylan (CBAX) is one of the most recalcitrant xylans for enzymatic degradation due to its particular heterogeneous nature. The present study aimed to investigate the capability of the filamentous fungus Penicilliumparvum 4-14 to enzymatically saccharify CBAX and reveal the fungal carbohydrate-active enzyme (CAZyme) repertoire by genome sequencing and secretome analysis.ResultsCBAX1 and CBAX2 with different branching degrees, together with corn bran residue (CBR) were generated from corn bran after alkaline hydrogen peroxide (AHP) pretreatment and graded ethanol precipitation. The protein blends E_CBAX1, E_CBAX2, and E_CBR were produced by the fungus grown on CBAX1, CBAX2, or CBR, respectively. Under the optimal conditions, E_CBAX1 released more than 80% xylose and arabinose from CBAX1 and CBAX2. Almost complete saccharification of the arabinoxylans was achieved by combining E_CBAX1 and a commercial enzyme cocktail Cellic®CTec3. Approximately 89% glucose, 64% xylose, and 64% arabinose were liberated from CBR by E_CBR. The combination of E_CBR with Cellic®CTec3 enhanced the saccharification of CBR, with conversion ratios of 97% for glucose, 81% for xylose, and 76% for arabinose. A total of 376 CAZymes including plentiful lignocellulolytic enzymes were predicted in P.parvum based on the fungal genomic sequence (25.8 Mb). Proteomic analysis indicated that the expression of CAZymes in P.parvum varied between CBAX1 and CBR, and the fungus produced complete cellulases, numerous hemicellulases, as well as high levels of glycosidases under the culture conditions.ConclusionsThis investigation disclosed the CAZyme repertoire of P.parvum at the genomic and proteomic levels, and elaborated on the promising potential of fungal lignocellulolytic enzymes upon saccharification of corn bran biomass after AHP pretreatment.

Utilizing rice straw and sugarcane bagasse as low-cost feedstocks towards sustainable production of succinic acid

Succinic acid (SA) has been produced from rice straw (RS) and sugarcane bagasse (SB) as low-cost feedstocks in this study through sequential peracetic acid (PA) and alkaline peroxide (AP) pretreatment assisted by ultrasound and pre-hydrolysis followed by simultaneous saccharification and fermentation (PSSF). The effect of yeast extract (YE) concentration, inoculum concentration, and biomass type on SA production was investigated. The results showed that SA production from RS and SB was significantly affected by the YE concentration. Final concentration and yield of SA produced were significantly increased along with the increasing of YE concentration. Moreover, inoculum concentration significantly affected the SA production from SB. Higher inoculum concentration led to higher SA production. On the other hand, SA production from RS was not significantly affected by the inoculum concentration. Using RS as the feedstock, the highest SA production was achieved on the medium containing 15 g/L YE with 5 % v/v inoculum, obtaining SA concentration and yield of 3.64 ± 0.1 g/L and 0.18 ± 0.05 g/g biomass, respectively. Meanwhile, the highest SA production from SB was acquired on the medium containing 10 g/L YE with 7.5 % v/v inoculum, resulting SA concentration and yield of 5.1 ± 0.1 g/L and 0.25 ± 0.05 g/g biomass, respectively. This study suggested that RS and SB are potential to be used as low-cost feedstocks for sustainable and environmentally friendly SA production through ultrasonic-assisted PA and AP pretreatment and PSSF.

Impact of different pretreatments on the anaerobic digestion performance of cucumber vine

Copious amounts of cucumber vine (CV) derived from crop growing and harvesting are casually discarded in the field, posing severely negative impacts on public health and the ecological environment. Treating CV via anaerobic digestion (AD) could represent a promising approach while the recalcitrant lignocellulosic structure restricts its conversion efficiency, thus underscoring the importance of valid pretreatments. This study systematically investigated the effects of nine types of commonly applied chemical pretreatments involved H2SO4, HCl, H3PO4, NaOH, KOH, Ca(OH)2, CaO, H2O2, and alkaline hydrogen peroxide (AHP) pretreatments on methane production of CV. Results showed that alkaline and AHP pretreatments were beneficial to the methane production of CV and obtained the considerable cumulative methane yield and biodegradability of 194.3-241.5mL·gVS-1 and 47.59-59.15%, respectively, 36.83-70.07% higher than untreated. Analyses of lignocellulosic compositions and structural characterizations revealed that alkaline and AHP pretreatments well destroyed both hemicellulose and lignin, which commendably increased the accessibility of cellulose, facilitating the methane production. The findings of this study provide not only efficient pretreatment methods for the disposal and utilization of CV during AD process but also promising alternatives for enhancing methane production performance of similar vine residues, which would be greatly valuable for industrial applications in the future.

Influences of organic loading, feed-to-inoculum ratio, and different pretreatment strategies on the methane production performance of eggplant stalk.

A large amount of eggplant stalk (ES) is incinerated after harvesting of eggplant every year, which aggravates environmental pollution and waste of resources. Converting ES into methane through anaerobic digestion (AD) technology may be a potential treatment method, considering the low environmental impact and high energy recovery. Firstly, this study explored the effects of organic loading (OL) and feed to inoculum ratio (F/I ratio) on the AD of ES by response surface methodology (RSM). In order to achieve higher AD efficiency, various pretreatments (acid, alkali, alkaline hydrogen peroxide (AHP), microwave, and ultrasound) were introduced and comprehensively assessed with regard to methane production, organic matter destruction, and kinetic parameters. Results showed that OL had a more significant impact on AD process compared to F/I ratio and methane production was enhanced remarkably when the OL and F/I ratio were 35.0g VS/L and 3.0, respectively. XRD, FTIR, and SEM analyses of pretreated ES showed that alkali and AHP pretreatments performed better in delignification. Under optimal conditions, the ES pretreated with 1.5% AHP (adjusted by KOH) performed the maximum methane production of 262.2mL/g VS with a biodegradability of 95.0%, which increased by 334.1% compared to untreated ES. This paper not only provides the theoretical data about methane production performance of ES but also gives practical guidance for efficient utilization of similar vegetable stalk biowastes, which is also promising for large-scale industrial applications in the future.

Enhanced removal of Cd2+ from water by AHP-pretreated biochar: Adsorption performance and mechanism

The sesame straw-derived biochar was successfully prepared via alkaline hydrogen peroxide (AHP) pretreatment in this study. Systematic experimental characterizations, 15 relevant batch and column adsorption models, combined with density functional theory (DFT) calculation were used to investigate the performances and micro-mechanisms of Cd2+ adsorption onto biochar. We found AHP-pretreatment could greatly improve the adsorption performance of biochar for Cd2+. The maximum Cd2+ adsorption capacity of AHP-pretreated biochar (87.13 mg g−1) was much larger than that of unpretreated biochar. Cd2+ adsorption was mainly dominated by the chemisorption of the homogeneous surface monolayer. The hydroxyl and carboxyl groups on the surface of biochar provided preferential adsorption sites, and liquid film diffusion and intra-particle diffusion were two dominant rate-controlling steps. Our results showed that ion exchange, co-precipitation, surface complexation, and Cd2+-π interaction were the dominant adsorption mechanisms. Especially, DFT calculations well-identified that lone-pair electrons during complexation and π electrons during coordination were provided by oxygen-containing functional groups and aromatic rings, respectively. The experimental breakthrough curves fitted better with the theoretical value of the BJP model, compared to Thomas, Yoon-Nelson, and EXY models. Overall, our study provides a promising method for Cd2+ removal from wastewater and resource utilization of agricultural wastes.

The bamboo delignification saturation point in alkaline hydrogen peroxide pretreatment and its association with enzymatic hydrolysis

A delignification saturation point (DSP) was observed for bamboo alkaline hydrogen peroxide pretreatment (AHP). Lignin removal was increased from 52.23% to ∼70% when increasing H2O2 dosage from 0% to 2% at the optimum pH, but it cannot be further reinforced as increasing the H2O2. With partial lignin preserved, the glucan hydrolysis yield was found to have a ceiling of ∼80%. This study indicated a strong association between enzymatic digestibility and lignin removal. Anatomical analysis by fluorescence microscope and confocal Raman microscope revealed that the undegradable lignin was mainly existing in the cell corner of sclerenchyma fibers, causing the DSP in the bamboo AHP. Finally, the residual lignin in pretreated bamboo was characterized with GPC, HSQC NMR, and 31P NMR, which revealed the nature of DSP. This study could help to understand the lignin modification during the AHP and further contribute to the establishment of a chemical-saving biorefinery.

Polyploid Miscanthus Lutarioriparius: A Sustainable and Scalable Biomass Feedstock for Cellulose Nanocrystal Preparation in Biorefinery

In this study, polyploid Miscanthus lutarioriparius (PML) was introduced as a new sustainable and scalable source for cellulose nanocrystal (CNC). The agronomic traits of PML were significantly different from Miscanthus × giganteus (MG), but their chemical components and physical features were similar. Notably, a remarkable co-extraction of hemicellulose, lignin and ash and non-crystalline cellulose was observed during crude cellulose isolation from PML than it from MG by modified alkaline peroxide pretreatment. In addition, subjecting crude cellulose of PML and MG biomass to sulfuric acid hydrolysis provided high-quality CNC. The analysis of particle size distribution, zeta potential, crystalline index, the degree of polymerization, SEM and yield potential suggested that the CNC extracted from PML showed higher stability, processability and productivity than that from MG. Therefore, it provides a new theoretical basis for the applications of CNC prepared by PML and MG. The results also revealed potential genetic approaches for Miscanthus spp. to enhance biomass and CNC yield.

Comparison between cold plasma, ultrasonication, and alkaline hydrogen peroxide pretreatments of garden waste to enhance humification in subsequent composting with kitchen waste: Performance and mechanisms

This study compared the performance and mechanisms of cold plasma, ultrasonication, and alkali-assisted hydrogen peroxide for garden waste pretreatment to advance humification in composting with kitchen waste. High-throughput sequencing integrated with Functional Annotation of Prokaryotic Taxa was used to relate bacterial dynamics to humification. Results show that all pretreatment techniques accelerated humification by 37.5% – 45.7% during composting in comparison to the control treatment. Ultrasonication and alkalization preferred to decompose lignocellulose to produce humus precursors in garden waste, thereby facilitating humus formation at the beginning of composting. By contrast, cold plasma was much faster and simpler than other pretreatment techniques to effectively disrupt the surface structure and reduce the crystallinity of garden waste to enrich functional bacteria for aerobic chemoheterotrophy, xylanolysis, cellulolysis, and ligninolysis during composting. As such, a more robust bacterial community was developed after cold plasma pretreatment to advance humification at the mature stage of composting.

Chemical and ultrastructural evolutions of corn stalk during sequential acid and alkaline hydrogen peroxide pretreatment

Two-step pretreatment, the sequential dilute acid pretreatment (DAP) and alkaline hydrogen peroxide pretreatment (AHP), is an efficient strategy to pretreat lignocellulose. Understanding the chemical and ultrastructural evolutions could improve the utilization of corn stalks. Advanced multiscale approach was integrated, to semi-quantitative elucidate the lignocellulose structural evolution. Implementing DAP followed by AHP offered greater accessibility to enzymes than the reverse order, with glucose yield reaching 57.1% under mild condition. DAP promoted lignin degradation in the following AHP, by partially solubilizing cell wall extracts from middle lamella, and relocating lignin to cell corner and lumen. This investigation can provide new insight into potential routes to further development of lignocellulose conversion.

Optimization of Acid-Catalyst Hydrolysis Process in Lactic Acid Production from Rice Husk by Using Lactobacillus bacteria

Lactic acid is an important component of manufacturing polylactic acid (PLA), which can be produced with high-lignocellulosic biomass, still few of them have utilized that. The production process starts with Alkaline Hydrogen Peroxide (AHP) pretreatment, acid-catalyzed (H2SO4) hydrolysis followed by post-hydrolysis, and fermentation using a mixture of Lactobacillus brevis + Lactobacillus rhamnosus and single Lactobacillus delbrueckii bacteria. The effectiveness of pretreatment results was analyzed by using Thermal Gravity Analysis (TGA), which resulted in the removal of lignin and cellulose are 32.60% and 43.18%, respectively. Lignin reduction results are supported by Scanning Electron Microscope (SEM) for morphology analysis; it is shown the surface of rice husk becomes rough and cracks after pretreatment. Cellulose reduction was analyzed by using X-Ray Diffraction (XRD) and shown the Crystallinity Index (CrI) decrease after chemical pretreatment. The optimum operating condition of hydrolysis was also studied using Face Centered Composite Design (FCCD) by Response Surface Methodology (RSM), and get the optimum conditions at a 4 M H2SO4 for 30.7 minutes 63.1 . Followed by post-hydrolysis for 10 minutes at 121 , it resulted in sugar concentration of 15.3056 g/L, which was analyzed by using dinitrosalicylic-acid (DNS) with Root Mean Square Error of 0.78009. The last process is fermentation which is carried out at 125 rpm 37 , for 48 hours. Through High-Performance Liquid Chromatography (HPLC) analysis, the lactic acid concentration of a single bacterium and a mixture was 24.595 and 24.975%, respectively. Similar research has been previously carried out using palm waste raw materials with the enzymatic hydrolysis method without optimization of RSM. This study investigates the effect of AHP pretreatment in the lignin removal process, the effectiveness of acid-catalyzed hydrolysis in the optimized sugar reduction through FCCD, and compares lactic acid concentrations obtained from the fermentation process with L. delbrueckii and culture L. rhamnosus + L. brevis.

Promising seawater hydrothermal combining electro-assisted pretreatment for corn stover valorization within a biorefinery concept

In this study, for the first time, seawater hydrothermal (SH) pretreatment combining subsequent electrogenerated alkaline hydrogen peroxide (EAHP) pretreatment was proposed to achieve an effective fractionation of corn stover into high value-added products. During SH pretreatment, complex ions in natural seawater (Mg2+, Ca2+ and Cl-) were used to promote depolymerization of xylan into xylo-oligosaccharides with 49.37% yield (190 °C,40 min), 18.52% higher than that of deionized water. Subsequent EAHP treatment not only provided a green and economical way to produce hydrogen peroxide but also synchronously realized satisfied delignification (94.91%). The integrated pretreatment resulted in 91.16% of glucose yield, which was about 5.6 times more than that of unpretreated corn stover. In addition, the recovered lignin fraction which has a potential application in functional materials were investigated by FTIR, 2D-HSQC NMR and GPC. In short, this work provided a novel and environmentally-friendly strategy for biorefinery-based fractionation of corn stover.