Grape (Vitis sp.) is a popular fruit crop and host for a large number of viruses. So far, more than 70 different viruses have been identified from grapevines (Martelli 2014). Recently, grapevine geminivirus A (GGVA; family Geminiviridae) has been identified from two table grape (Vitis vinifera) cultivars, Black Beet and Nagano Purple (Al Rwahnih et al. 2017). Moreover, GGVA has been identified from grapevines in China (Fan et al. 2017). In 2016, we collected five grapevine leaf samples from 16 individual plants representing 12 different cultivars. All 15 different grapevine plants were grown at the Grape Research Institute, Okcheon, Korea, and Shine Muscat (V. labruscana Bailey × V. vinifera) was obtained from a vineyard in Okcheon. Shine Muscat displayed leaf malformations, vein clearing, and yellowing, while the other cultivars did not display any observable disease symptoms. Sixteen different libraries for RNA sequencing without pooling were prepared and subjected to paired-end sequencing (2 × 100 bp) using Illumina’s HiSeq 2000 followed by bioinformatics analyses, as described previously (Jo et al. 2016). Of 16 plants, 27 GGVA-associated contigs were identified from eight plants representing six cultivars: 188-08 (V. monticola × V. riparia) (two plants), Shine Muscat (two plants), Jaok (Vitis hybrid), Jungrang (Vitis hybrid), Jarang (V. vinifera), and Cabernet Sauvignon (V. vinifera). The numbers of reads associated with GGVA were 67 (188-08, #1 plant), 146 (188-08, #2 plant), 36 (Cabernet Sauvignon), 296 (Jaok), 118 (Jarang), 492 (Jungrang), 419 (Shine Muscat, #1 plant), and 171 (Shine Muscat, #2 plant). To confirm the results of RNA sequencing, we obtained five GGVA complete genome sequences (GenBank MF163261–65) from five cultivars by PCR using two abutting primer pairs (GGVAv950/GGVAc961 and GGVAv1402/GGVAc1438) and recovered the full GGVA genome, which was followed by Sanger sequencing (Al Rwahnih et al. 2017). The GGVA isolates 188-08 and Jaok shared 99% identity with isolate Tamar (KX618694.1), while isolates Cabernet Sauvignon, Jarang, and Shine Muscat shared 99% identity with known GGVA isolates: Koshu Sanjaku (KX570617.1), Scolokertek Kiralynoje (KX570618.1), and Black Beet (KX570609.1), respectively. The possible presence of GGVA in the other eight plants representing six cultivars—Okrang (Vitis hybrid), Campbell Early (V. labruscana), Chungporang (Vitis hybrid), Alicante Bouschet (V. vinifera), Cabernet franc (V. vinifera), and Chardonnay (V. vinifera)—was tested by PCR. PCR was conducted for 16 plants using GGVA-specific primers for amplifying the coat protein region of 414 bp in size, GGVA-1370F1 5′-TGTAAAGAGATCAGCCCAAATGTTTTC-3′ (position 1,370 to 1,396), and GGVA-1783R1 5′-ATGCAATTTTCGTCTCCCTGCA-3′ (position 1,783 to 1,762) based on the GGVA reference genome (NC_031340.1). The PCR results demonstrated that all 16 plants were infected by GGVA, while RNA sequencing revealed the presence of GGVA in eight plants. Although a previous study reported a low rate of GGVA infection in the U.S.A. (Al Rwahnih et al. 2017), a previous study showed that GGVA was widely present in grapevines in China (Fan et al. 2017). Our results demonstrated that GGV can infect diverse Vitis spp., including V. labruscana and other Vitis hybrids, and the genome sequences of five GGVA isolates were highly conserved. Taken together, this is the first report of GGVA infecting diverse Vitis spp. in Korea.