Reference evapotranspiration is crucial for estimating plant water needs and managing resources. Simplifying the Penman-Monteith model by reducing input parameters and local calibration can enhance efficiency and reliability. In this study, three empirical formulas—Hargreaves and Samani (HS, 1985), Priestley and Taylor (PT, 1972), and the World Meteorological Organization (WMO, 1966)—representing temperature-, solar radiation-, and mass-transfer-based approaches, respectively, were evaluated. To achieve this objective, meteorological data from five synoptic stations situated in the Samsun province in the Black Sea region of Türkiye, were utilized. Three methods, namely the traditional method, regression analysis, and genetic algorithm, were employed to estimate the local calibration coefficients of empirical equations based on the Penman-Monteith 56 equation. Finally, the outcomes were evaluated based on four criteria: root-mean-square error, coefficient of determination, mean bias error, and percentage error of estimate. The results of empirical formulas both before and after calibration were analyzed. Prior to calibration, HS and PT exhibited greater accuracy for the case study. This accuracy trend was also observed in the calibrated results. Additionally, among the three employed calibration methods, regression analysis and traditional methods demonstrated a higher level of accuracy.