Alfalfa Extract Research Articles

Study of the Effects of Alfalfa Bioactive Substances and Polysaccharides on the Lactation Performance and Immune Function of Dairy Cows

Background: The aim of this study was to investigate the effects of alfalfa polysaccharides on the lactation performance and immune function of dairy cows. Methods: A total of 84 healthy Holstein cows were selected and randomly divided into 7 groups, including the control Group A0, the alfalfa polysaccharide groups (AP1, AP2, AP3) and the alfalfa extract groups (AE1, AE2, AE3). Each group had three replicates and each replicate had four cows. The daily rations of the dairy cows in the AP group and the AE group were supplemented with alfalfa polysaccharide powder and alfalfa extract at concentrations of 1, 2 and 3 kg/t, those in the A0 group served as a control. Result: (1) the milk production of dairy cows in the AE groups was extremely significantly greater (p less than 0.01) than that in A0 group and AP groups. The milk protein content of cows in the AP2 and AE2 groups was significantly greater (P less than 0.05) than that in the A0 group (2) The interleukin-2 (IL-2) levels in the serum of cows in the AP2, AP3, AE2 and AE3 groups were greater than those in the A0 and AP1 groups and the IgM antibody levels in the AP group and the AE group were extremely significantly greater than those in the A0 and AP1 groups (P less than 0.01). The IgA antibody level in the serum of dairy cows in the AP2, AP3, AE2 and AE3 groups was significantly (P less than 0.01) greater than that in the A0, AP1 and AE1 groups. In summary, the alfalfa extract improved both the lactation performance and immunity of dairy cows, indicating comprehensive effects. Alfalfa polysaccharides at a concentration of 2 kg/t improved the immunity of dairy cows.

Study the Effect of Aqueous Extract of Alfalfa a and Clove as Natural Antioxidant on Some Meat Quality Characteristics in Common Carp Cyprinus carpio

Study the Effect of Aqueous Extract of Alfalfa a and Clove as Natural Antioxidant on Some Meat Quality Characteristics in Common Carp Cyprinus carpio

Green Synthesis, Characterization and Biological activity of Optimized Alfalfa‐Mediated Zirconium Nanoparticles

AbstractNanoparticle green synthesis is an emerging scientific field that focuses on the production of nanoparticles by living cells. In this work, the potential of extracts of alfalfa leaves (Medicago sativa) as affordable and fabricated zirconium nanoparticles (ZrNPs) was evaluated for the first time. alfalfa alcoholic extract and zirconium precursor were used to produce ZrNPs, the production and characteristics of nanoparticles were measured using the Tyndall effect, UV‐vis, FT‐IR, EDX, DLS, and SEM tests, antioxidant and antibacterial properties, and toxicity Measurement of nanoparticles on breast cancer (MDA‐MB‐468) and human healthy cells (HFF) was investigated. The optimization of nanoparticle production was evaluated with ANN and GN algorithms. The analysis showed that nanoparticles have spherical‐like ZrNPs shapes with an average of 50 nm size. in addition, ZrNPs have significant cytotoxic effects against human breast cancer (MDA‐MB‐468) cells, and Human foreskin fibroblast (HFF) cells exhibited the highest resistance to ZrNPs. There was a significant agreement between the results of the designed ANN‐GA model and the experimental data. In this research, ZrNP was successfully created using alfalfa extract as a renewable source of stabilizing and reducing agents with an environmentally friendly approach, and they showed significant antioxidant, antibacterial, and anticancer properties.

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Study on the Key Autotoxic Substances of Alfalfa and Their Effects.

Alfalfa is a leguminous plant with strong autotoxicity, which seriously affects regeneration and stability. In order to clarify the relationship between the key autotoxic substances and autotoxicity of alfalfa, this experiment determined the content of phenolic autotoxic substances in different varieties of alfalfa and the effect of different concentrations of alfalfa extracts on seed germination, seedling growth and physiology. The results showed that the content of single autotoxic substances in the eight alfalfa varieties was highest for total coumarin. The variety with the highest total coumarin content was "LZ", and the lowest content was "656". Principal component analysis of the autotoxicity of eight alfalfa varieties revealed that the variety with the strongest autotoxicity was "LZ" and the weakest was "656". After treatment with extracts, the germination potential, germination rate, germination index and vigor index of 656 were higher than those of LZ, and the seeds of LZ and 656 did not germinate when the concentration was higher than C0.025 and C0.05, respectively. Compared with LZ, 656 had stronger osmotic regulation and antioxidant capacity, while the degree of membrane lipid peroxidation and ROS accumulation were lower. Further correlation analysis between the autotoxic substance content and autotoxicity observed that the content of total coumarin and autotoxic substances showed a significant positive association with autotoxicity (p < 0.01), and the total coumarin content showed a significant positive correlation with the content of autotoxic substances (p < 0.05). The total coumarin content is the major contributor to autotoxicity, and the higher the coumarin content, the higher the autotoxic substance content and the stronger the autotoxicity. Eight alfalfa varieties were systematically clustered on the basis of total coumarin content and autotoxicity, and the high-autotoxic alfalfa variety "LZ" and low-autotoxic alfalfa variety "656" were screened.

Qualitative and quantitative analysis for the content of benzoic, salicylic, sorbic and glycyrrhizic acids in an aqueous extract of alfalfa

The article presents the results of experimental studies on the assessment of the content of benzoic, salicylic, sorbic and glycyrrhizic acids in the aqueous-alcoholic extract of alfalfa (Medicago sativa L.) and in its condensed extract (BAA «Erakond»), belonging to the class of preservatives of natural origin. Qualitative and quantitative analysis was carried out by high performance liquid chromatography (HPLC) on a Waters Breeze chromatograph (Waters, USA). The content of acids endowed with antiseptic and fungicidal properties in the air-dry raw material of alfalfa was, mg/g: benzoic – 3.2 ± 0.3; salicylic – 1.8 ± 0.2; sorbic – 0.15 ± 0.11; glycyrrhizin – 2.0 ± 0.3. The technology of hydrobaric extraction, used in the industrial production of the dietary supplement «Erakond» from alfalfa, ensured the concentration of the analyzed acids in the finished product: the content of benzoic acid in the dietary supplement «Erakond» was 26.0 ± 3.0 mg/g, salicylic acid – 2.9 ± 0.3 mg/g, sorbic – 0.22 ± 0.03 mg/g, glycyrrhizin – 5.2 ± 0.6 mg/g. The mechanisms of implementation of the antioxidant properties of polyphenolic compounds of alfalfa, which exhibit a synergistic preservative effect together with identified organic acids, are disclosed. Relatively high concentration of polyphenolic compounds in Erakond dietary supplement (naringenin – 0.05 ± 0.0006; rutin – 8 ± 3; licuraside – 3.5 ± 0.4; quercetin – 0.04 ± 0.001; fisetin – 0.35 ± 0.01; naringin – 0.5 ± 0.0006; dihydroquercetin – 0.20 ± 0.002) provides high stability of Eracond-containing food products during long-term storage with preservation of nutritional, taste and biological properties. The combined antiseptic and antioxidant action in vitro of organic acids, polyphenolic compounds, essential oils, coumarins and plant saponins ensures the effectiveness of the use of natural preservatives in food industry technologies.

Dietary alfalfa hay or lipid-soluble alfalfa extract may improve broiler growth, but fiber presence may be detrimental during Eimeria vaccine challenge

Lipid-soluble components in late-cutting alfalfa are linked to beneficial immune and microbiota responses in mouse challenge models; therefore, responses in a comparative poultry Eimeria challenge model were investigated. The study objective was to evaluate performance, immunity, and the cecal microbiota in broilers fed ground hay or lipid-soluble extract from late (fifth) cutting alfalfa during Eimeria challenge. At hatch, 432 Ross 708 broilers were placed in 24 floor pens (18 birds/pen) and assigned to 3 isocaloric/isonitrogenous dietary treatments consisting of control, 5% ground hay, or 0.25% lipid-soluble extract for a 42-d trial divided into 14 d starter, grower, and finisher periods. On d 14, 4 birds/treatment were euthanized to collect blood and cecal contents before half the remainder were inoculated with 10X Merck Coccivac-B52 (Kenilworth, NJ). Tissue samples were collected at 3, 7, 14, and 28 d postinoculation (pi; 4 birds/diet×Eimeria group) with body weight (BW) and feed intake (FI) recorded weekly. Immune populations within peripheral blood mononuclear cells were characterized by flow cytometry while cecal microbial communities were profiled by 16S rRNA gene amplicon sequencing. Data were normalized when appropriate and analyzed to evaluate the effects of diet, Eimeria challenge, and timepoint (SAS 9.4; P ≤ 0.05). Before challenge, dietary alfalfa hay or extract increased FI 6.9 to 8.0% and increased CD3+ T cells 19.3 to 24.9% compared to control-fed birds (P ≤ 0.007). Alfalfa did not significantly affect post-Eimeria performance, but Eimeria-challenged birds fed hay showed the greatest numeric reduction in final BW compared to their unchallenged counterparts (0.17 kg) vs. control and extract-fed birds (0.02-0.04 kg). Immune cell changes did not indicate recruitment from peripheral blood to local infection sites; however, alfalfa hay may have accelerated Bu-1+ B cell development by 7 d in unchallenged birds (P < 0.0001). During Eimeria-challenge, dietary alfalfa extract preserved alpha diversity measures related to species richness (P ≤ 0.007). Collectively, these results indicate potential benefits of feeding lipid-soluble extract from late cutting alfalfa to broilers during Eimeria challenge.

In Vitro Bioactivities of Commonly Consumed Cereal, Vegetable, and Legume Seeds as Related to Their Bioactive Components: An Untargeted Metabolomics Approach Using UHPLC-QTOF-MS2.

We conducted a comprehensive evaluation of the antioxidant, anti-obesity, anti-diabetic, and anti-glycation activities associated with the consumption of broccoli, red cabbage, alfalfa, and buckwheat seeds. Additionally, we explored the relationship between these biological activities and the profiles of amino acids, polyphenols, and organic acids identified in the seeds. Our findings demonstrated that red cabbage, broccoli, and buckwheat extracts exhibited significantly higher antioxidant potential compared to the alfalfa extract. Moreover, buckwheat displayed the most significant capacity for inhibiting alpha-glucosidase. Remarkably, broccoli and red cabbage demonstrated substantial anti-glycation and lipase inhibitory potentials. We identified the presence of amino acids, polyphenols, and organic acids in the extracts through untargeted metabolomics analysis. Correlation analysis revealed that pyroglutamic acid positively correlated with all the investigated functional properties. Most polyphenols made positive contributions to the functional properties, with the exception of ferulic acid, which displayed a negative correlation with all tested biological activities. Furthermore, gluconic acid and arabinonic acid among the organic acids identified displayed a positive correlation with all the functional properties. These results strongly support the anti-diabetic, anti-obesity, and anti-glycation potential of red cabbage, broccoli, and buckwheat seeds.

Effect of aqueous extracts of the sprouted seeds on the quantitative and qualitative yield of the coral lettuce cultured under the modified nutrient solution film NFT system

The study was conducted at the fields of the Dept. of Horticulture and Garden Engineering, College of the Agricultural Engineering Sciences, Jadriyah in the fall season of 2020-2021 aiming to culture the coral lettuce with green and red leaves under the hydroponics system using the modified nutrient solution film NFT and study the effect of aqueous extracts of alfalfa and berseem sprouted seeds on the quantitative and qualitative yield of the lettuce crop. The research was conducted as an experiment of split plots within the Randomized Complete Block Design (RCBD) of three replicates. The seedlings of the green coral lettuce, Locarno RZ, and red coral lettuce, Locarno RZ, symbolized by A and B respectively, were transferred to the culture system on 1/10/2020. Lettuce hybrids were regarded as the less important factor (main plot). The plants were sprayed with aqueous extracts of the alfalfa sprouted seeds at the two concentrations of 5 and 10%, symbolized by T1 and T2, and with aqueous extracts of the berseem sprouted seeds at the two concentrations of 5 and 10%, symbolized by T3 and T4, respectively, in addition to the control treatment (spraying with distilled water only), symbolized by T0. They were sprayed to full wetness three times at an interval of 10 days. This was regarded as the most important factor (subplot). Each experimental unit contained 10 plants. Results showed significant superiority of the treatment AT2 in the leaf chlorophyll content, number of leaves, leaf area and dry weight of vegetative growth (80.00 mg.100g fresh weight-1, 37.00 leaf.plant-1, 144.05 dcm2.plant-1, 10.97 g.plant-1 respectively) compared to the lowest value of the treatment BT0 (60.56 mg.100g fresh weight-1, 19.00 leaf.plant-1, 63.32 dcm2.plant-1 and 5.65g.plant-1 respectively). The same treatment (AT2) was superior in the highest root dry weight, and plant yield amounted to 1.92 and 364.35g.plant-1, respectively compared to the lowest values at the treatment AT0, recording 0.75 and 219.92g.plant -1 respectively. The interaction treatments' effect was insignificant on the content of β-carotene and VC in the leaves. We concluded that it is possible to culture the coral lettuce hydroponically and increase its productivity through spraying with the aqueous extracts of the alfalfa sprouted seeds at the concentration of 5%, which did not record a significant difference in the production from the treatment of spraying with aqueous extracts of the alfalfa sprouted seeds at 10%. Keywords: Lactuca sativa L., Red lettuce, Organic nutrients, Plant extracts, Soil culture.

Effects of Horsetail, Alfalfa, Ortie, Chêne and Aleppo oak as Potential Hemostatic Agents on Laboratory Coagulation Tests

This study investigated the effect of ethanol extracts of horsetail, alfalfa, ortie, chêne and aleppo oak on blood coagulation in vitro. Extraction was performed by the maceration method. Extracts were mixed with platelet and plasma, then prothrombin time (PT), activated partial thromboplastin time (APTT) and platelet aggregation tests were conducted. Alfalfa extract had a dose-dependent effect on the PT. Ortie, and horsetail, reduced the PT significantly compared to control group. Alfalfa, horsetail, and ortie reduced the APTT, but their effect was insignificant compared to the control group. The pooled extract showed the highest effect compared to the single extracts in a dose-dependent manner. Horsetail and alfalfa induced platelet aggregation in response to arachidonic acid but not in response to collagen. In the case of ortie, no aggregation occurred regarding the arachidonic acid, and incomplete was observed in response to collagen. Interestingly, blood clotting occurred immediately after adding the chêne, aleppo oak and the pooled extract, and therefore platelet poor plasma (PPP) and platelet rich plasma (PRP) became jelly. Generally, chêne and aleppo oak, as well as pooled extract, were more effective in inducing both primary and secondary coagulation pathways via shortening the PT and APTT, and induction of platelet aggregation.

The Effect of Alfalfa (Medicago sativa) extract onthe number of macrophage, fibroblast, and amount of collagen in rat wound healing

Background: Alfalfa extract has been shown to have anti-inflammatory activity in wound healing. Objectives: This study aims to investigate the effect of Alfafa on the number of macrophages, fibroblast, and collagen in the rat wound healing. Method: In this post-testonly control group design study, 50 Wistar rats were randomly divided into five groups. K0 (negative control) group was treated with ointment base. K1 (positive control) group was treated with gentamicin. P1, P2, and P3 groups were treated with 10%, 20%, and 30% alfalfa extract respectively. The termination of rats conducted on day 3 and day 7 post of first treatment (5 rats/group/period). On day 3, the number of macrophage and fibroblast were evaluated using Hematoxylin-Eosin staining. On day 7, the number of collagen was evaluated using Siriusred staining. The data were analyzed using one way ANOVA followed by Tukey’s post hoc test. Result: The result, showed a significant difference in the number of macrophages between the negative control treated group and K1 groups. The number of fibroblasts was significantly different between the P3 group and the P1, K1 group. There was a significant difference in the number of collagens between negative control andtreated group, and between the positive control and P2, P3 group. Conclusion: The administration of alfalfa extract at the dose of 20% can accelerate wound healing by increasing the number of fibroblasts and collagen fibers and decreasing number of macrophages. Bangladesh Journal of Medical Science Vol. 22 No. 01 January’23 Page : 77-83

Growth promoting activity, anti-biofilm effect, and down regulation of papC and rcsA genes expression by Medicago sativa (alfalfa) extract

Consumption of Medicago sativa (alfalfa) has been linked to infection control in livestock. Therefore, studying the mechanism of this action may lead to the development of enriched food supplements. The purpose of this study was to investigate how M. sativa extract affects the growth of Lacticaseibacillus casei, Lactiplantibacillus plantarum, Staphylococcus aureus, and Escherichia coli. The effects of M. sativa extract on the bacterial cell adherence to Caco-2 cells (Human colon epithelial adenocarcinoma cell lines) and expression of genes involved in the formation of E. coli biofilm were also studied. Generally, in concentrations lower than 100 mg/ml, alfalfa extract had no antimicrobial effect on the probiotic and pathogenic bacteria. However, it not only acted as a growth promoter for two probiotic strains at 2.5% concentration but also increased the antibacterial effect of probiotics supernatants. Furthermore, alfalfa extract reduced the adhesion of pathogenic bacteria to Caco-2 cells. M. sativa extract was identified to inhibit the biofilm formation by E. coli through decreasing the genes expression, papC, and rcsA. As a result, it looks that alfalfa can improve the growth and function of beneficial bacteria, but further studies are needed to elucidate its mechanism of action.

Protein recovery and quality of alfalfa extracts obtained by acid precipitation and fermentation

Alfalfa has a high potential as a novel food protein and can contribute to meet the increasing food demand. To facilitate food grade quality, protein extraction is essential. In this study, different extractions with isoelectric focusing the protein were compared; acid precipitation (citric and lactic acids) and fermentation (endogenous and Lactobacillus salivarius). Acid precipitation resulted in the highest protein recovery (30 %) compared with L. salivarius and endogenous fermentation. The protein ribulose-1.5-bisphosphate carboxylase/oxygenase (RuBisCO) was found intact and in high concentrations in acid precipitated samples. With L. salivarius, a higher content of peptides and possible cross-linked proteins were discovered. However, for both extractions, the amino acid composition showed a promising distribution meeting the recommendation of the Food and Agriculture Organization (FAO). Overall, these results indicate that protein extracts obtained with acid precipitation might be more appropriate as a food ingredient, whereas fermented products might have potential as food supplement.

Green nanopriming: responses of alfalfa (Medicago sativa L.) seedlings to alfalfa extracts capped and light-induced silver nanoparticles

The application of nanotechnology in agriculture can remarkably improve the cultivation and growth of crop plants. Many studies showed that nanoparticles (NPs) made plants grow more vigorously. Light can make NPs aggregated, leading to the reduction of the NPs toxicity. In addition, treatment with NPs had a “hormesis effect” on plants. In this study, light-induced silver nanoparticles (AgNPs) were synthesized by using the alfalfa (Medicago sativa L.) extracts, and then the optimal synthetic condition was determined. Light-induced AgNPs were aggregated, spherical and pink, and they were coated with esters, phenols, acids, terpenes, amino acids and sugars, which were the compositions of alfalfa extracts. The concentration of free Ag+ was less than 2 % of the AgNPs concentration. Through nanopriming, Ag+ got into the seedlings and caused the impact of AgNPs on alfalfa. Compared with the control group, low concentration of light-induced AgNPs had a positive effect on the photosynthesis. It was also harmless to the leaf cells, and there was no elongation effect on shoots. Although high concentration of AgNPs was especially beneficial to root elongation, it had a slight toxic effect on seedlings due to the accumulation of silver. With the increase of AgNPs concentration, the content of silver in the seedlings increased and the silver enriched in plants was at the mg/kg level. Just as available research reported the toxicity of NPs can be reduced by using suitable synthesis and application methods, the present light induction, active material encapsulation and nanopriming minimized the toxicity of AgNPs to plants, enhancing the antioxidant enzyme system.Graphical

An Evaluation of the Activity of Prepared Zinc Nanoparticles with Extracted Alfalfa Plant in the Treatment of Heavy Metals

The alfalfa plant, after harvesting, was washed, dried, and grinded to get fine powder used in water treatment. We used the alfalfa plant with ethanol to make the alcoholic extract characterized by using (GC-Mass, FTIR, and UV) spectroscopy to determine active compounds. Alcoholic extract was used to prepare zinc nanoparticles. We characterized Zinc nanoparticles using (FTIR, UV, SEM, EDX Zeta potential, XRD, AFM). Zinc nanoparticle with Alfalfa extract and alfalfa powder were used in the treatment of water polluted with inorganic elements such as Cr, Mn, Fe, Cu, Cd, Ag by (Batch processing). The batch process with using alfalfa powder gets treated with Pb (51.45%), which is the highest percentage of treatment. Mn (13.18%), which is the lowest percentage of treatment. The batch process with using Zinc nanoparticles gets the result treated with Pb(98.822%), which is the highest percentage of treatment, and Mn (10.31%), which is the lowest percentage of treatment. When comparing alfalfa powder and zinc nanoparticle, it has been found that the treatment with zinc nanoparticle is more efficient in the removal of inorganic pollutants.

Protein Recovery and Quality of Alfalfa Extracts Obtained by Acid Precipitation and Fermentation

Protein Recovery and Quality of Alfalfa Extracts Obtained by Acid Precipitation and Fermentation

Alfalfa saponins inhibit oxidative stress-induced cell apoptosis through the MAPK signaling pathway

ABSTRACT Background: Oxidative stress could seriously affect the growth performance of piglets. As natural extracts of Alfalfa (Medicago sativa), alfalfa saponins have been shown to function as antioxidants in piglets in vivo. However, few studies have investigated the effects and mechanism of alfalfa saponins against oxidative stress in piglet cells in vitro. In the current study, piglets’ small intestinal epithelial cell line (IPEC-J2) was explored to investigate the protective effects of alfalfa saponins on injured cells induced by H2O2. Methods: To investigate the effects and mechanism of alfalfa saponins against oxidative stress in piglet cells, the cell viability, activity of antioxidant enzymes, LDH and the amount of MDA were detected in H2O2-treated cells after the cells were pre-incubated with alfalfa saponins. The mechanism of alfalfa saponins against H2O2-induced oxidative cell damage was explored by detecting the expression of mitochondrial apoptosis-related proteins. Furthermore, the signaling pathway of alfalfa saponins in IPEC-J2 cells under oxidative stress was also investigated. Results: The results indicated that alfalfa saponins could rescue cell viability, elevate the activity of antioxidant enzymes and down-regulate the activity of LDH and the amount of MDA in H2O2-induced cells. Conclusion: Alfalfa saponins could inhibit oxidative stress-induced cell mitochondrial apoptosis through the MAPK signaling pathway, thereby providing a new method for improving antioxidant stress ability by means of nutritional regulation.

The Antioxidant Effect of Medicago sativa L. (Alfalfa) Ethanolic Extract against Mercury Chloride (HgCl2) Toxicity in Rat Liver and Kidney: An In Vitro and In Vivo Study.

Heavy metals such as mercury are some of the environmental pollutants and can induce toxicity by bioaccumulation and oxidative damage. This study aimed to investigate the effect of ethanolic extract of Medicago sativa L. (Alfalfa) on mercury damage in the kidney and liver of rats. Thirty Wistar rats were randomly divided into five groups, the control group, S group (2 mg/kg mercury chloride), and T1, T2, and T3 groups that, in addition to mercury, received doses of 250, 500, and 750 mg/kg of the alfalfa extract. On the last day, blood samples were taken, and the serum was separated to measure biochemical and oxidative stress parameters in the kidney and liver. A part of the kidney and liver was also used for histopathological evaluation. Total phenols and flavonoids were 40.45 ± 2.12 and 14.36 ± 0.45 mg/g, respectively, whereas IC50 was 245.18 ± 19.76 μg/ml. The body weight significantly decreased in the S group compared to other groups, while treatment with different doses of alfalfa extract increased the body weight. Mercury concentration in the kidney was higher than that in the liver. The serum levels of urea, creatinine, alanine aminotransferase (ALT), and alkaline phosphatase (ALP) significantly increased in the S group compared to the control group, while treatment with different doses of alfalfa extract increased their levels. Moreover, an increase in malondialdehyde (MDA) and a decrease in glutathione peroxidase (GPx), catalase (CAT), total antioxidant capacity (TAC), and superoxide dismutase (SOD) activity were observed in the S group. The level of these parameters significantly improved in the groups receiving the extract compared to the S group. Furthermore, the histopathological evaluation showed glomerular and tubular damage and hepatic necrosis in the S group and that these conditions improved in the T3 group. The findings of this study showed that the ethanolic extract of alfalfa in a dose-dependent manner has potentially unique protective effects against mercury poisoning in the kidney and liver.

Comparative Studies of Selected Criteria Enabling Optimization of the Extraction of Polar Biologically Active Compounds from Alfalfa with Supercritical Carbon Dioxide

The aim of this research was to provide crucial and useful data about the selection of the optimization criteria of supercritical carbon dioxide extraction of alfalfa at a quarter-technical plant. The correlation between more general output, including total phenolics and flavonoids content, and a more specified composition of polar constituents was extensively studied. In all alfalfa extracts, polar bioactive constituents were analyzed by both spectrometric (general output) and chromatographic (detailed output) analyses. Eight specific phenolic acids and nine flavonoids were determined. The most dominant were salicylic acid (221.41 µg g−1), ferulic acid (119.73 µg g−1), quercetin (2.23 µg g−1), and apigenin (2.60 µg g−1). For all seventeen analyzed compounds, response surface methodology and analysis of variance were used to provide the optimal conditions of supercritical fluid extraction for each individual constituent. The obtained data have shown that eight of those compounds have a similar range of optimal process parameters, being significantly analogous for optimization based on total flavonoid content.

Analysis of individual biologically active compounds from root crop extracts of purple alfalfa (medicago sativa) in vitro

The authors selected the parameters for obtaining root cultures in vitro Medicago sativa. The number of cell culture lines studied is 15. The duration of the growth cycle is less than 50 days. The number of processed explants is 75. The growth characteristics of root cultures of in vitro purple alfalfa (Medicago sativa) were studied. The growth index for the dry biomass of the in vitro root culture of alfalfa was 31. A qualitative and quantitative analysis of the composition of BAS in the biomass of the obtained root cultures of in vitro alfalfa (Medicago sativa) was carried out. Unique biologically active substances (not previously described for alfalfa) were identified.): apigenin, naringenin, myricetin, chrysoeriol, coumestrol, and scopoletin. Rational parameters of isolation of individual BAS from the extract of root cultures of in vitro alfalfa (Medicago sativa) were selected. The degree of extraction of individual BAS is 80%. Methods of purification of individual BAS Medicago sativa have been developed. The degree of purification of individual BAS is 95%.