Continuing an investigation of the composition of bitumen from Aleksinac (Yugoslavia) shale, we report here in the bitumen extracted with benzene the following compounds: aromatic hydrocarbons, CnH2n−14 (biphenyls) and CnH2n−18 (anthracenes and/or phenanthrenes), oxygen compounds CnH2nO2 (methyl esters of fatty acids), CnH2nO (aliphatic methyl ketones), and the triterpenoid ketone adiantone. The presence of these compounds in the bitumen is due to diagenetic changes of precursor biological material; adiantone alone presumably represents an intact residue of Miocene ferns.