Alcoholic Extract Research Articles

Efficacy of aqueous and alcoholic formulations extracted from Padina boryana algae against Aedes aegypti mosquito larvae: A comparative study

AbstractAedes aegypti is a mosquito species known for transmitting several diseases, with dengue fever being of utmost importance. Controlling the spread of dengue fever remains a significant global health challenge. Other diseases transmitted by Ae. aegypti include Zika virus, chikungunya, and yellow fever. Padina boryana algae were collected from the Red Sea coast south of Jeddah to evaluate the effectiveness of its alcoholic and aqueous extracts against Ae. aegypti mosquito larvae. The algae sample was washed, dried in the shade, processed, and subjected to aqueous and alcohol extraction using a rotary evaporator. The resulting extracts were tested for their larvicidal activity against Ae. aegypti mosquito larvae using standard bioassay techniques. The larval mortality rates were recorded, and the lethal concentration (LC50 and LC90) values were calculated.The results revealed that both the aqueous and alcoholic extracts of P. boryana algae exhibited significant larvicidal activity against Ae. aegypti mosquito larvae. However, the alcoholic formulation demonstrated higher efficacy compared to the aqueous formulation. The LC50 values for the alcoholic and aqueous extracts were 194.8 and 195.7 ppm, respectively. The LC90 values for both extracts were 653.1 and 656.7 ppm, respectively. This indicates the effectiveness of Padina extracts as an effective pesticide against the larvae of the Ae. aegypti mosquito. Overall, the alcohol extract showed promising efficacy, indicating its potential as a significant component in developing effective mosquito control strategies.

COMPARATIVE PHYTOCHEMICAL EVALUATION AND ANTIMICROBIAL ACTIVITY STUDIES ON MEDICINAL TREE AEGLE MARMELOS (L.) CORREA AND ITS INTRASPECIFIC VARIANT EKABILVA

The present study is focused on the comparative phytochemical analysis and antimicrobial activity in the typical form of the Aegle marmelos(AM1) and its intraspecific variant EkaBilva(AM2). Since ancient times Bilvais highly used in the indigenous systems of medicinesfor various disorders but extensively for dysentery and diabetes.Its intraspecific variant EkaBilvahas more extensively used rather than Bilva by the locals in Telugu speaking states. To find out the variations in the phytochemical components and their activity in the both typical form and intraspecific variant the studies were carried out. The powdered leaf materials of both were extracted by using Aqueous, ethanol, chloroform and pet-ether extracts. The following chemical compounds were reported Alkaloids, Steroids, Triterpenoids,Tannins, Glycosides, Flavonoids, Reducing sugars, Phenolsin AM1&AM2. Tannins, Flavonoids are present in Alcohol extracts in AM1 differing with AM2. The antimicrobial activity was carried out by disk-diffusion method by using four different microorganisms with the nutrient agar medium the high zone of inhibition shown in the leaf extracts of AM2 in all extracts except aqueous in all isolates.

Elucidating the mechanism of hepatotoxicity in Euodia rutaecarpa: insights from QSAR toxicity prediction and metabolomics

Objective: Euodia rutaecarpa, (Wu Zhu Yu) a Chinese medicine clinically used to treat gastrointestinal disorders, has been widely employed. However, Euodia rutaecarpa is regarded as a small toxic traditional Chinese medicine in the Chinese Pharmacopoeia and other herbal works. Using toxicity predictions combined with in vitro and in vivo studies, this study aimed to identify the toxic components and toxic target organs of Euodia rutaecarpa, and explore its toxic mechanism from a metabolic perspective. Methods: The toxic target organs of Euodia rutaecarpa were identified through in vitro and in vivo studies. In vitro toxicity screening was performed by alkaloid enrichment and isolation. The potential toxicity of compounds was predicted by Absorption, Distribution, Metabolism, Excretion, and Toxicity Predictor (ADMET Predictor) based on Quantitative Structure–Activity Relationship (QSAR) construction. In addition, the study integrated the serum metabolomic analysis after the administration of potentially toxic components to clarify the effect of potentially toxic substances on metabolism in mice. Results: Comparing the acute toxicity in mice of different extraction methods and before and after processing, it was evident that Euodia rutaecarpa alcoholic extract had the highest toxicity, and the target organ of Euodia rutaecarpa toxicity was the liver. The alkaloid fraction of alcoholic extract of Euodia showed strong cytotoxicity. The potential toxicity of Euodia rutaecarpa was calculated and predicted by ADMET Predictor, and alkaloids are suspected to be responsible for the toxicity of Euodia rutaecarpa. Evodiamine significantly reduced the number of cells and increased the mitochondrial membrane potential in vitro. Different metabolites were significantly identified by serum metabolomics, of which bile acid metabolism and steroid hormone biosynthesis are the key pathways of hepatotoxicity. Conclusions: Clarify the scientific significance of clinical use of processed products by comparing the acute toxicity of different extraction methods before and after processing. Combining the toxicity prediction based on QSAR with the toxicity screening in vitro and in vivo, the potential toxic target organs and toxic components of Euodia rutaecarpa can be identified. Through metabolomics, we preliminarily revealed that the hepatotoxicity of Euodia rutaecarpa may be related to bile acid metabolism and steroid hormone biosynthesis. This study lays the foundation for elucidating the mechanism of Euodia rutaecarpa and evaluating its safety and quality.

FEATURES OF THE APPLICATION OF AROMATHERAPY IN A PRESCHOOL EDUCATION INSTITUTION AND AT HOME WITH PRESCHOOL CHILDREN WITH THE PURPOSE OF IMPROVING THEIR HEALTH

The article describes the essence of aromatherapy, reveals its principles, features and methods of application with preschool children in order to improve their health. The importance of applying non-traditional methods of preschool children’s health improvement, in particular aromatherapy, is emphasised. The author describes the history of aromatherapy, which dates back to ancient cultures. It is noted that with the advent of distillation, it became possible to use alcohol and water-alcohol plant extracts. It is noted that aromatherapy has become widespread over the past twenty years. The article analyses the works of researchers, which indicate the effectiveness of aromatherapy in preserving the health of preschool children and speech therapy with preschoolers. It is noted that aromatherapy involves treatment with essential oils contained in plants. It is indicated that aromatherapy is effective in regulating the functioning of internal organs and body systems. It is noted that essential oils affect the human body in two ways: through the respiratory system and through the skin. The rules for selecting and using essential oils are revealed. The method of aromatherapy application is described. The basic principles of using aromatherapy with preschool children are presented. It is noted that the use of aromatic oils will be effective through the use of various devices and a list of them is provided. The ways of using aromatherapy with preschool children in preschool education institutions and at home are described. Contraindications to the use of aromatherapy with children are indicated. It is emphasised that aroma oils are effective in improving physical and mental health. The article describes the indications for the use of essential oils. It is noted that aromatherapy is a universal means of preserving and promoting children’s health and can be used by educators and parents in various activities (educational, play, work, independent) both in preschool education institution and at home. It has been noted that aromatherapy is also effective in disinfecting premises where children are staying.

Isolate and Diagnose Negative Bacteria that Cause Urinary Tract Inflammation and Test their Sensitivity to Certain Antibiotics and Rheum Palmatum

The current study included the collection of 50 diarrhoea samples from UTIs, ages 25-50 years, 60% female and 40% male patients reviewed to Kirkuk city laboratories, gave 40 positive germ growth samples and 80%, while 10 other samples gave no germ growth. Isolation and diagnosis results showed that E.coli was prevalent on Proteus mirabilis and at 75%, while Proteus mirabilis was at 25% lower. The results of the study showed isolated bacterial sensitivity to a number of antibiotics and the results showed isolated bacterial sensitivity to anti-Ciprofloxacin while bacterial isolates showed the highest resistance to antibiotics. Effective compounds were identified and their concentration in the Riwas plant was determined using High Performance Liquid Chromotography HPLC (containing phenolic, turbine and alkaloid compounds, aquatic and alcoholic extracts were tested at concentrations (25), (50) and (100) and the focus (25) was on the lowest toxins.

Detection of active compounds in Aloe vera leaf extract and their inhibitory effect on Pseudomonas aeruginosa.

In the study, two types of Aloe vera leaf extracts were prepared: aqueous and alcoholic extract. The research was conducted at the Department of Life Sciences, Faculty of Science, Tikrit University. From October to January. The active compounds in these alcoholic extracts were detected as tannins, cladosides, saponins, and flavonoids, all of which gave a positive result. The antibacterial activity of the aqueous and alcoholic extracts were estimated at concentrations ranging from (200-12. 5) mg/ml, where the alcoholic extract gave a high efficacy of (34.5) mm, while the aqueous extract had a lower efficacy (29.6) mm at the same concentration. Microbial tests were evaluated using different combinations of the extracts on Pseudomonas aeruginosa isolates, where the alcoholic extract gave the highest inhibitory efficacy to the bacteria.

Coordination of bioactive phytochemicals from Aloe vera extracts to metal ions; investigation of the metal complexes and bioactive compound formed.

The bioactive compounds contained within many plants account for their pharmacological values. Aloe vera has a wide range of organic and inorganic components, including carbohydrate polymers, glucomannans, and a variety of other natural and synthetic materials. The study aims to take a look into the characteristics of some metal complexes produced from Aloe vera extracts. The extracts from Aloe vera were derived by means of acetone, distilled water and ethanol. The solubility of the metal complexes with the ligand at varying temperatures was established. FT-IR was used to carry out the infra-red examination of the ligand. The results revealed that alcoholic extract of Aloe vera leaf was not soluble in Cu, Fe, or Zn but only soluble in Fe, the extract by distilled water was soluble in Cu, Fe and Zn. However, the Aloe vera in acetone as well as in the Zn (II) and Cu (II) composites displayed a bending that was found at 1430.97cm-1, 1500.01cm-1 and 1615.90cm-1.every functional groups are assigned to be coordinating sites as a result of increase or decrease in the wave number, and absorption band. Findings from the investigation reveal that the complexion of the metal salts with diverse donor sites in the extract is indicated by an increase in the absorption peak of the functional groups in the metal composites of the extracts.

Piscicidal Effects of Extracts of Karenja Plant Pongamia pinnata (L.) Pierre and Vat plant Clerodendrum viscosum (Vent.) on singhi Fish Heteropneustes Fossilis (Bloch)

The piscicidal properties of the seed, leaf and bark of the indigenous plants, karenja Pongamia pinnata (L.) Pierre and vat Clerodendrum viscosum (Vent.) were studied on the predatory fish Singhi, Heteropneustes fossilis (Bloch) under laboratory conditions. Distilled water, 50% ethyl alcohol and absolute ethyl alcohol extracts of the plant parts of the plants were tested in this experiment. The altered behaviour due to the effects of the extracts was noted, initially hyperactivity, losing stamina and in the later period, after a few hours, the affected fishes becoming sluggish and inactive. Mortality data varied depending upon the toxicity of the plant parts, extracts and concentrations. On the basis of concentrations, the relative toxicity of the piscicides on H. fossilis for P. pinnata was found to be in the order of seed > bark > leaf and for C. viscosum, seed > leaf > bark. The toxicity of seed and leaf extracts of C. viscosum was greater than P. pinnata but opposite for bark extract for the fish. On the basis of LC50 values the toxicity of both the plant parts were similar as to seed > bark > leaf. The Chittagong Univ. J. B. Sci.,Vol. 7(1 &2):65-78 , 2012.

Using molecular fine structure to identify optimal methods of extracting fungal glycogen

Glycogen is a highly branched glucose polymer that is an energy storage material in fungi and animals. Extraction of glycogen from its source in a way that minimizes its molecular degradation is essential to investigate its native structure. In this study, the following extraction methods were compared: sucrose gradient density ultracentrifugation, thermal alkali, hot alcohol and hot water extractions. Molecular-size and chain-length distributions of glycogen were measured by size-exclusion chromatography and fluorophore-assisted carbohydrate electrophoresis, respectively. These two fine-structure features are the most likely structural characteristics to be degraded during extraction. The results show that the thermal alkali, hot alcohol and hot water extractions degrade glycogen molecular size and/or chain-length distributions, and that sucrose gradient density ultracentrifugation with neither high temperature nor alkaline treatment is the most suitable method for fungal glycogen extraction.

Investigation of the in vitro and in vivo therapeutic activity of Tarantula cubensis extract (Theranekron®) on Leishmania major.

Cutaneous leishmaniasis (CL) is a vector-borne parasitic disease caused by several species of the protozoan parasite Leishmania. The need for new anti-leishmanial drugs for the treatment of CL is highlighted by factors such as high cost, toxicity, potential for resistance and limited long-term use of existing anti-leishmanial drugs. The aim of this study was to investigate the therapeutic effect of Tarantula cubensis alcoholic extract (TCE), which has been shown to have wound-healing, anti-inflammatory, regenerative, resolving and epithelialising effects, on L. major promastigotes in vitro and in vivo in an experimental mouse model of CL. The effect of TCE on L. major promastigotes in vitro was investigated after determination of non-cytotoxic concentrations of TCE using the XTT method. To establish a CL model, L. major amastigotes were injected into the paws of BALB/c mice. Lesion size and histopathological evaluation were used to assess the effect of treatment. TCE was found to be effective against L. major promastigotes at 24 h and 48 h at concentrations of 250 μg/mL, 125 μg/mL and 62.5 μg/mL (P<0.05). TCE was found to be more effective than meglumine antimonate in treating CL in the experimentally induced CL model in BALB/c mice. The results suggest that TCE holds promising potential therapeutic agent for the treatment of CL. However, further extensive investigations are required to substantiate and expand understanding in this area.

Hibiscus Sabdariffa L. Extract as a Natural Additive in Food Packaging Biodegradable Films to Improve Antioxidant, Antimicrobial, and Physicochemical Properties.

In this study, biodegradable active films were prepared from potato starch and polyvinyl alcohol at different proportions, mixed with acetone extract of Hibiscus sabdariffa L. (HS) and using glycerol as a plasticizer. Functional properties, antimicrobial, and antioxidant capacity were evaluated. Potato starch films with a proportion of polyvinyl alcohol up to 50% and HS extract had significant antioxidant capacity and antibacterial effect against most of the analyzed strains. Adding polyvinyl alcohol (PVOH) and HS extract improved the mechanical performance and reduced water vapor permeability of the materials. The active biobased films with HS extract presented good physicochemical, antimicrobial, and antioxidant properties. These materials are considered as suitable for food packaging, and the active compounds in the roselle extract are a natural antibacterial option for the food area. The materials based entirely on biodegradable products are an excellent alternative when developing and marketing biobased materials, minimizing the environmental impact of food packaging.

Vallaris solanacea induces mitochondrial mediated apoptosis in HL-60 human promyelocytic leukemia cells

In the present study, the apoptosis-inducing potential of a chloroform fraction from an alcoholic extract of Vallaris solanacea aerial parts (VS) was examined using human promyelocytic leukemia HL-60 cells. We discovered a concentration and time-dependent decrease in cell growth using MTT assay. Scanning electron micrographs and fluorescence microscopy were used to observe several well-documented morphological and nuclear alterations, such as reduction in cell size, chromatin condensation, fragmentation, and the creation of cell surface blebs. A considerable rise in the Sub-G0 population was revealed by cell cycle analysis. Additionally, a dose-dependent rise in cells positive for Annexin V was observed. DCFH-DA test on VS-treated HL-60 cells showed an increase in endogenous ROS generation of up to 4.3 fold. Additionally, suppression in Bcl-2 levels and increased mitochondrial membrane depolarization in treated cells were also associated with a rise in cytosolic cytochrome-c levels that was consequently followed by the activation of the caspase cascade. Further, the DNA fragmentation assay exhibited a typical ladder formation at 25 μg/ml, which became prominent in a concentration-dependent manner. Our study revealed that VS has apoptosis-inducing potential towards HL-60 cells in vitro and is an effective candidate for further anti-cancer studies.

Whitening and antioxidant activities of Inonotus sanghuang Extract and development of primary whitening gel products.

Polysaccharides and flavonoids have excellent antioxidant properties and tyrosinase inhibitory effects. In this paper, the antioxidant capacity of Inonotus sanghuang extract and its inhibition kinetics on mushroom tyrosinase were investigated to determine the preparation process of Inonotus sanghuang primary whitening gel. By conducting experimental studies on the effects of water extract and alcohol extract of Inonotus sanghuang on antioxidant capacity and tyrosinase activity, their inhibitory ability and types of inhibitory effects were determined. The single factor experiment was used to determine the preparation process of Sanghuang primary whitening gel. This study has proven that the extract of Inonotus sanghuang possesses antioxidant and tyrosinase inhibitory capabilities. It also identified the preparation process for the primary whitening gel of Inonotus sanghuang, thereby providing a theoretical and experimental basis for the development of whitening products utilising Inonotus sanghuang.

Identification and Analysis of Ardisia humilis as Potential Antihyperlipidemic by Network Pharmacology Followed by Molecular Docking

Objective: Hyperlipidemia is increasing lipids in a body that are risk factors for cardiovascular disease that increased last over 30 years. Natural products have a large portion as pharmacological agents, particularly in disease therapies. The pharmacological activity of natural product remedies has been thoroughly screened using high approaches in drug discovery. Lampeni, also known as Ardisia humilis Vahl, is utilized for various illnesses such as vertigo, rheumatism, and skin ulcers, and as a stimulant, carminative, andantidiarrheall.Previous studies have shown that feeding Swiss albino rats alcohol extract at 200mg/KgBW could decrease LDL, triglycerides, total cholesterol, and VLDL and increase HDL. Method: This study aimed to predict Lampenileaf's network pharmacology as a potential for hyperlipidemiausing multiple ethnobotanical databases and software. This research was cond.This is in silico. Results: The result of Lampeni leaf is almost all bioactive compounds targeted hyperlipidemia-associated genes.Compounds with the highest potential of Hyperlipidemia are Ardisinol II, Bilobol, ArdisiphenolB, Maesaquinone, Beta amiryn, and Embelin. IL6, HSP90AA1, EGFR, MAPK3, SRC, PPARG, and STAT3 had the best value and became the gene doth the Lampeni leaf compound.These targets are tightly associated with inflammatory and proliferation processes, which explain the possible explaining Lampeni leaf in attenuating hyperlipidemic symptoms.Further study is needed to validate the result other than by molecular docking method, such as molecular dynamics, in vitro, or in vivo studies.

An anti-inflammatory active ingredients in Poriae cutis: Screening based on network pharmacology.

Hyperuricemia (HUA) is a disorder of uric acid metabolism, which can lead to the formation of gouty arthritis, kidney inflammation and other damages. Previous studies have found that the alcohol extract of Poria cutis can reduce the level of uric acid and protect against kidney injury. Based on network pharmacology, the core targets and main active components of P. cutis intervention in HUA were determined. Most of the potential active ingredients are triterpenoid acids such as tumulosic acid (TA) and eburicoic acid (EA), and the potential targets are TNF and IL-6, which are associated with inflammation. In vitro experiments have shown that TA can significantly inhibit the release of NO, TNF-α and IL-6 in inflammatory RAW264.7 cell culture medium and the expression of TNF-α and IL-6 in RAW264.7 cells. This study suggests that TA based on network pharmacological screening has obvious anti-inflammatory effect on inflammatory RAW264.7 cells and is a promising anti-inflammatory compound.

The effect of alcoholic extract of Citrullus colocynthis on the structural manifestations of the parasite Fasciola gigantica and its comparison with the drug pendazole

The current study aims to find out the effect of alcoholic extracts of the fruit of the Citrullus colocynthis plant, as it was during the current study during the period from 12/2/2022 to 24/4/2023 and by 206 livers for cows, including (31) infected livers and 1348 livers for sheep that did not show infection. In the current study, the incidence of giant liver Fasciola gigantica cows was 15.04%, while sheep hadn't infection during the study period. The results of the current study show that there are different effects in the tissues of the treated worms if compared to the control group, which was showing the integrity of the tissues well after 6 hours, as it is observed the layers of the body wall, spines and muscle layers that cover the wall, as well as the parenchyma tissue in which the body systems are embedded, which did not notice any changes in those tissues and structures, which include (for intestinal backfilling, testicles, mules and uterus). As noted, the damages vary according to the concentration of separation and deformation in the longitudinal and circular muscles, and a few cells are seen in the tenderloin. The thorns are still present in the cover, and the effect of the drug albendazole is little practical Clearly.

Effects of Phyllanthus niruri antiseptic skin cream on surgical wounds in Felis catus after ovariosalpingohysterectomy

Studies highlight the cost-effectiveness and lower adverse effects of herbal remedies than allopathic medicines, making them attractive amidst growing microbial resistance concerns. This investigation explored the in vitro antiseptic properties of Phyllanthus niruri alcoholic extract (EA) against Escherichia coli, Staphylococcus aureus, Streptococcus spp., and Candida albicans. Various in vitro methods were employed, including disk diffusion, Minimum Inhibitory Concentration (MIC), and Bactericidal/Fungicidal Concentration (MBC/MFC). A skin cream formulation using P. niruri was developed, undergoing in vivo trials on ten feline subjects post-ovariosalpingohysterectomy (OSH). Felines were treated with P. niruri skin cream (100mg/ml), with 0.1% aqueous chlorhexidine as the positive control. Skin assessments included hyperemia, crust, edema, and exudation. Inhibition was observed in vitro at various EA concentrations, and cytotoxicity analysis showed no harm. The P. niruri skin cream at 100mg/ml exhibited consistent outcomes, with a minor deviation from the fifth day onwards, without compromising therapeutic efficacy (Tukey test, p &lt; 0.05). The formulation demonstrated stable physical-chemical properties with no adverse feline reactions. These findings suggest the skin cream's in vivo antimicrobial potential as a future antiseptic alternative.

Mechanism of action of Sambucus williamsii Hance var. miquelii in the treatment of osteoporosis analyzed by UHPLC-HRMS/MS combined network pharmacology and experimental validation

Sambucus williamsii Hance var. miquelii(SWH) is a precious wild Chinese herb whose fruit, rhizome, leaves and root bark can be used as medicine. Sambucus Linn has pharmacological effects such as anti-osteoporosis, promoting fracture healing, anti-viral and anti-inflammatory. In this study, the main chemical components of the alcoholic extracts from SWH were rapidly identified by ultra-high performance liquid chromatography-quadrupole orbit trap high-resolution mass spectrometry (UHPLC- HRMS MS), and a total of 42 compounds were characterized from the alcoholic extracts of SWH. The results of network pharmacological validation showed that kaempferol, quercetin, luteolin, isorhamnetin and morroniside were the main active components, and KEGG enrichment demonstrated that SWH mainly affected the signaling pathways such as PI3K-Akt, TNF and FoxO by modulating the related targets such as AKT1, PIK3R1, EGFR, RELA SRC and PTGS2. The molecular docking results showed binding solid activity between the main active components of SWH and the targets. The network pharmacology was validated by establishing an animal model of osteoporosis (OP) in rats by gavage administration of vitamin A acid. The results of the pharmacological experiments showed that SWH could improve the degree of bone loss in the femur of osteoporotic rats, increase the number of trabeculae and decrease trabeculae porosity, up-regulate the Ca and P content in the serum of OP rats, down-regulate the scope of ALP and BGP in the serum, and promote the calcification of the bone matrix, and then exert the anti-OP efficacy. In this study, network pharmacology and pharmacological experiments verified the pharmacological mechanism of SWH in anti-OP rats. This provides a theoretical basis for the research and development of anti-OP drugs and a reference for the application of other traditional Chinese medicines in treating OP diseases.

The effect of alcoholic extract of brown algae on obesity complications in rats: neurological and histological studies

The effect of alcoholic extract of brown algae on obesity complications in rats: neurological and histological studies

Phytochemicals, antioxidant ability and in vitro anthelmintic activity of crude extracts from Vitexnegundo leaves against Haemonchus contortus.

The aim of this study was to evaluate the in vitro anthelmintic effect of crude aqueous, methanol, ethanol, hydro alcohol and acetone extracts of Vitex negundo leaves against Haemonchus contortus eggs and larvae. Phytochemical analysis to identify the number of compounds in extracts was done by chemical tests and gas chromatography coupled to a mass spectrophotometer detector (GC-MS). First off all the effectiveness of dried plant materials was evaluated on larval development by mixing powdered material (no nano particles) to faecal cultures from donor sheep. Adding powder to the faecal culture resulted into 100% inhibition in larval development at 200 and 300mg/g of faeces. The anthelmintic activity was assessed using the egg hatch assay (EHA) and the larval mortality assay (LMA). Comparison of mean inhibition percentage of egg embryonation, mean inhibition percentage of egg hatching and mean percentage of larval mortality at different concentrations with control was performed by one-way ANOVA. The means were compared for statistical significance using DMRT at P < 0.05. For both the assays, 50% inhibitory concentration (IC50) and lethal concentration (LC50) were calculated by probit analysis. Chemical test revealed presence of high concentration of saponin and flavoinoids and moderate concentration of total phenols in leaves. The antioxidant activity (radical scavenging activity, RSA %) measured was 35.47%. On GC-MS, the methanolic leaves extract revealed 30 phyto-compounds. On EHA, there was marked effect on inhibition of egg hatching by aqueous, hydro alcohol and acetone extracts. On LMA all the five extracts showed excellent larvicidal activity. V. negundo leaves methanol extract mediated silver nanoparticles were found very effective at much lower concentrations as compared to crude methanol extract. The results indicated that the V. negundo leaves crude extracts possessed excellent in vitro ovicidal and larvicidal properties against H. contortus which needs more investigation, especially in vivo trials for the control of parasite.