Eleven neoglycolipids were synthesized with a membrane anchor (cholesterol or Guerbet alcohols) attached, via a hydrophilic spacer, to a potential lectin-recognized sugar moiety ( N-acetylglucosamine: GlcNAc). Neoglycolipids G24-0, G28-0 and G32-0, with a C 24, C 28 and C 32 Guerbet alcohol residue, had no spacer, while neoglycolipids G24-3, G28-3, G32-3 contained a triethoxy spacer. Cholesteryl neoglycolipids chol-1 to chol-4 and chol-7 contained one to four and seven ethoxy units. All the cholesteryl neoglycolipids were incorporated into liposomes, while, for the Guerbet derivatives, the presence (or not) of a spacer and the length of their alkyl chains played an important role for obtaining mixed liposomes. The abilities of liposomes to be recognized by wheat germ agglutinin (WGA) were measured as an increase of the absorbance at 450 nm. Significant WGA-induced aggregations were obtained with liposomes containing neoglycolipids chol-3, chol-4, chol-7, G28-3 and G32-3. As neoglycolipid G24-3 was not recognized, the accessibility of its GlcNAc moiety for WGA depended not only on the spacer length but on the nature of the anchor. The WGA-induced aggregations increased with increasing lectin concentrations until a maximum value, which depended on the nature of the neoglycolipid. The highest aggregation obtained with G28-3 and G32-3 occurred for a WGA–neoglycolipid mole ratio superior to the ratio observed with chol-3, chol-4 and chol-7. A preincubation of WGA with free GlcNAc did not inhibit the aggregation between WGA and mixed liposomes, demonstrating a greater affinity of WGA for the neoglycolipid GlcNAc than for free GlcNAc.