Alcohol Extractive Values Research Articles

Incorporation of standardised extract of Curcuma longa Linn into phytosomes and its evaluation for in vitro Anti-inflammatory potential and Brine shrimp lethality assay

Aim: To incorporate standardized Curcuma longa Linn extract into phytosomes and evaluate for in vitro anti-inflammatory and BSL bioassay. Method: The quality of the plant material was determined by various pharmacognostic parameters. The plant material was then subjected to maceration for extraction using ethanol: water as solvent followed by Soxhlet extraction. The resulting extract was subjected to phytochemical analysis to determine the presence of plant metabolites. The drug and excipients compatibility was evaluated by FTIR study. Furthermore, using the thin film hydration approach, a new lipid-based phytosome was prepared. In vitro anti-inflammatory and brine shrimp lethality tests were performed on prepared phytosome. Results: Moisture content, total ash, acid insoluble ash, water soluble ash values, aqueous, alcohol, and petroleum ether extractive values are all found to be within limits. The phytochemical analysis validated the existence of alkaloids, tannins, resins, carbohydrates, proteins, flavonoids, and saponins. The compatibility study demonstrates the compatibility of excipients with drugs. Thin film hydration technique was employed successfully to prepare the phytosomes containing Curcuma longa linn extract. In vitro anti-inflammatory activity revealed that prepared phytosome could serve as natural based therapeutic option for anti-inflammatory potential. Brine shrimp lethality assay also confirmed the bioactivity of prepared phytosomes. Conclusion: The method used for standardization can be used to aid with plant identification and quality analysis of Curcuma longa Linn for future research. It can be inferred from the findings that phytosomes loaded with Curcuma longa Linn extract exhibited promising anti-inflammatory and cytotoxic effects.

Pharmacognostic Evaluation of the Leaves of Coccinia barteri Hook F (Cucurbitaceae)

Background: In general, Coccinia barteri is offered and sold in its raw form. This natural form could be adulterated, substituted, or contaminated. Therefore, the purpose of this study is to use pharmacognostic analyses to verify the authenticity of the leaves of the Coccinia barteri plant.
 Methods: The organoleptic studies were carried out through sensory organs. Histological analyses were conducted by microscopic examination of the specimen mounted on hand slides. Phytochemical screening and chemomicroscopic evaluation were also carried out using various standard methods.
 Results: The leaves of Coccinia barteri come in a range of forms and sizes. They are lustrous, bright, and dark green. They are also tiny and feature opposite imparipinnate compound leaves. The organoleptic evaluation showed a leafy odour and an astringent taste. Microscopic evaluation showed glandular trichomes, wavy epidermal cells, and anomocytic stomata. The leaf had anomocytic stomata and hypostomatic. with an average stomatal number, index, density, length, width and size of 13.75 ± 0.40%, 15.44 ± 0.42 mm-2, 80.88±2.82 mm-2, 29.26µm, 26.17 ± 0.4 µm and 766.38± 39.49 µm, respectively.
 The phytochemical screening revealed the presence of phenols, terpenoids, saponins, alkaloids, proteins, glycosides, and flavonoids, while tannins and fixed oils were not detected. Chemotaxonomic studies showed the presence of starch grains, lignified tissues, calcium oxalates, gum, and the absence of protein and oil globules.
 Analytical evaluation of the leaves of Coccinia barteri produced 7.00% total ash, 0.93% acid insoluble ash, 2.96% water soluble ash, 8.40% moisture content, 29.67% alcohol extractive value, 40.17% water extractive value, and 21.00% ethyl acetate extractive value.
 Conclusion: This study provides information on the morphology, microscopic, and phytochemical profile of the leaves of Coccinia barteri.

Pharmacognostic and Preliminary Phytochemical Investigation of Clerodendrum thomsoniae Leaves

Clerodendrum thomsoniae (Lamiaceae) is an ornamental plant grown almost worldwide in humid environment. The phytocompounds present in plant protect the body from oxidative stress, brain disorders, skin rashes, sores and depression. Present study deals with to establish pharmacognostic, physiochemical and phytochemical standards of Clerodendrum thomsoniae leaves. Microscopical evaluation of Clerodendrum thomsoniae leaves showed the presence of covering trichomes, epidermis and other characteristics. Results of Clerodendrum thomsoniae leaves yielded 7.5% w/w total ash, alcohol extractive value not more than 23.33% w/w (Hot extraction), 21.66% w/w (Cold maceration) and water extractive value not more than 30.12% w/w (Hot extraction), 36.66% w/w (Cold maceration) and 8% w/w total moisture content by LOD. The Phytochemical investigation of extracts of Clerodendrum thomsoniae leaves shows the presence of sterols, triterpenes, alkaloids, flavonoids, tannins, glycosides, proteins and carbohydrate. Pharmacognostical and phytochemical parameters determined in the present work can serve as major criteria for identity, quality and purity of a crude drug and extracts.

Pharmacognostic Study and Hepatoprotective Activity of the Methanolic Extract and Fractions of Leaves of Picralima nitida Apocyanaceae

Picralima nitida the only species in the genus Picralima belongs to the Apocyanaceae family. It is widely known for its medicinal purposes. The aim of the study was to investigate pharmacognostic parameters of the leaf and evaluate the hepatoprotective activity against carbon tetrachloride induced hepatotoxicity using Swiss Albino mice. The physicochemical evaluation indicated 11.75% moisture content, 8.50% total ash, 9.50% acid insoluble ash, 4.00% water soluble ash, 13.75% alcohol extractive value and 11.00% water extractive value. Macroscopic analysis on the fresh leaves revealed an odourless green plant with bitter taste. Microscopic examination indicated the presence of calcium oxalate crystals, starch grains, epidermal cells, xylem, parenchyma cells, paracytic stomata and palisade tissue. Chemomicroscopic evaluation indicated the presence of oxalate crystals, starch grains, lignified tissues, tannins, cellulose, protein and oil. The acute toxicity result revealed that P. nitida had no adverse effect in Swiss Albino mice. The ethyl acetate fraction had hepatoprotective ability on liver enzymes (alanine transaminase, aspartate aminotransferase, alkaline phosphatase) and can produce the same result as ascorbic acid (standard).

FORMULATION AND EVALUATION OF KALYANAKA KSHARA: AN AYURVEDIC COMPOUND FORMULATION

Background: Ayurveda utilises different forms of herbs in therapeutics. Kshara is one such form. The material destroys or cleans the excessive/morbid doshas (Ksharanat Kshananat va Kshara). They are considered anushastra’s (para-surgical instruments). Kshara’s (alkaline substances) are more suitable and can be used where shastra chikitsa (surgery) is contraindicated. Kshara (alkaline substances) is the best drug for internal and external use. Considering the quick action, lesser dose, and more comprehensive action, an attempt is made to formulate and standardise the product, as there is insufficient data regarding its standardisation. Objective: To formulate and standardise the Kalyanaka Kshara. Methods: All ingredients were taken per the reference Ashtanga Hridaya chikitsa sthana, Kalyanaka Kshara. All the Raw materials like plants, animals, and mineral drugs were collected from the local market of Jhansi, Uttar Pradesh. And by the sharava samputa (earthen pot) method, three different samples (KK1, KK2, and KK3) of Kalyanaka Kshara were prepared. Result: Physicochemical changes in each sample, organoleptic characters of the product yield were observed. The standard values of Kalyanaka Kshara are alkaline with pH 10.01, loss on drying (% w/w) 0.47, total ash value (% w/w) 55.5, acid insoluble ash value (% w/w) 0.93 water extractive value (% w/w) 62.55, alcohol extractive value (% w/w) 38.54. Conclusion: On behalf of each sample's pharmaceutical and physicochemical analysis, there are no considerable changes in any of the KK1, KK2, and KK3 samples, and encouraging results were observed. The developed SOP and physicochemical parameters can be adopted as pharmacopeial standards.

Pharmacognostic standards for Uvaria chamae P. Beauv. leaves (Annonaceae)

Background : Standardisation as a tool in quality control ensures purity, safety and efficacy of herbal medicines. Uvaria chamae from the family Annonaceae has wide medicinal application which includes its use as a febrifuge and as an anti-inflammatory.Objective: The study was aimed at developing some Pharmacognostic standards for the leaves of U. chamae.Material and Methods: Uvaria chamae plant was collected, identified and authenticated. Fresh leaves were examined macroscopically and microscopically based on standard procedures. Fluorescence behavior was assessed by adding various reagents and viewing at daylight, 254 nm and 365 nm. Chemomicroscopy and physicochemical analysis of powdered samples were done using standard procedures. Phytochemical and Thin layer chromatographic analysis of the extracts were also carried out.Results: Leaves of U. chamae are alternately arranged and elliptic in shape. The abaxial surface has straight polygonal epidermal cells with paracytic stomata. Vascular bundles are arranged in fan shape from the transverse section of the leaf. Chemomicroscopic evaluation revealed the presence of lignin, starch and oil. Alcohol and water soluble extractive values were 4.00 ± 0.00 and 5.6 ± 0.67, respectively. The membrane stabilization activity of the extracts at 0-1 mg exceeded that of the standard drug diclofenac. The ethyl acetate fraction had the highest percentage of inhibition of haemolysis at 0.4 mg.Conclusion: The features established in this study may contribute in the identification and quality control of U. chamae.

Quality standardization, Phytosome formulation and in vitro antioxidant activity of Moringa oleifera Lam: An Ayurvedic medicinal plant

Aim: To formulate and evaluate Moringa oleifera loaded phytosome for its in vitro antioxidant activity. Method: The plant material was analyzed for its quality by analyzing various pharmacognostical properties. The plant material was subjected for extraction by using maceration followed by soxhlet extraction using ethanol: water as solvent. The resultant extract was subjected for phytochemical investigations to assess the presence of plant metabolites. Furthermore, novel lipid based nano formulation such as phytosome was prepared by thin film hydration method. The prepared phytosomes loaded with Moringa oleifera extract was analysed for DSC, FTIR, particle size and entrapment efficiency. The optimized formulation (F3) was subjected for in vitro antioxidant activity. Thin Layer Chromatography was performed using Quercetin as standard and Methanol: Water as mobile phase. Results: The organoleptic evaluation revealed the plant is green, aromatic odour, slightly bitter taste erect stems, thick grey bark, flowers are white. Physicochemical parameters such as Moisture content, Total ash, Acid Insoluble ash, Water soluble ash values, Aqueous, Alcohol and Petroleum ether extractive values are found to be 8.5, 10.5, 5, 12.5, 12.5 and 2.5% respectively. The phytochemical investigation confirmed the presence of Alkaloids, Glycosides, Steroids, Terpenoids and Phenols. The compatibility study confirms that the excipients and drugs are compatible with each other. Among four prepared phytosomes with different ratios, formulation 3 exhibited smaller particle size such as 141nm and entrapment efficiency as 81%.Hence, formulation 3 is subjected for further antioxidant activity. With the IC50 value of 17.82µg/ml, formulation 3 showed promising antioxidant activity which was compared with standard ascorbic acid with IC50 33.53 µg/ml. Conclusion: The present work can be utilized for plant identification and standardization of Moringa oleifera for further research .Based on the results, it can be concluded that novel lipid based nanoformulation loaded with Moringa oleifera exhibited promising Antioxidant properties. The limitations arising from conventional dosage forms will be overlooked by the novel lipid based phytosomes with greater bioavailability and remarkable therapeutic effects.

Anti-inflammatory and Analgesic Investigations of Methanol Extract of Ganoderma lucidum

Ganoderma lucidum is a popular woody and spongy mushroom (fungi) widely distributed throughout the world. It is commonly used in the production of nutriceuticals, functional foods and also serves as a therapeutic herb used in the treatment of several diseases. This study was aimed at evaluating the phytochemicals, proximate composition, antioxidant, anti-inflammatory and analgesic activities as well as acute toxicity of the crude methanol extract of G. lucidum. The phytochemicals, proximate composition and antioxidant potential were determined using already established methods. The formalin-induced inflammation and acetic acid-induced writhing techniques were applied to evaluate the anti-inflammatory and analgesic activities respectively. Phytochemicals detected were saponins, flavonoids and terpenoids. The moisture content, acid insoluble ash, water soluble ash, total ash, alcohol extractive value and water extractive value were 12.53 ± 0.18%, 1.45 ± 0.21%, 2.68 ± 0.51%, 3.31 ± 0.2%, 1.41 ± 0.00% and 1.07 ± 0.01% respectively. The IC50 values for the DPPH radical scavenging capacity of the extract and ascorbic acid standard) were 31.56 ± 1.30 and 18.84 ± 2.06 µg/mL respectively. The crude extract at the dose of 50 mg/kg body weight showed the highest % inhibition of edema after 4 hours and there was a significant decrease (p < 0.05) in the number of writhes in a dose dependent manner. In the oral administration of the crude extract to Swiss mice, 100% mortality was recorded at 5000 mg/kg. The study confirms that G. lucidum is a potential source of phytomedicine with substantial pharmacological and antioxidant properties but however, could be toxic at higher doses.

Phytocheical and antidiabetic evaluation of the solvent fractions of <em>Coccinia barteri</em> Hook F (Cucurbitaceae) on alloxan induced diabetic rats

The use of natural anti-diabetic medications in treating diabetes is receiving more attention daily. This study evaluated the phytochemical constituents and anti-diabetic properties of the leaves of Coccinia barteri on alloxan induced diabetic rats. The powdered leaf was macerated in methanol for 72hrs and then fractionated with ethyl acetate, chloroform and aqueous methanol solvents. Phytochemical and analytical evaluations were done using standard methods. The toxicity study was carried out on mice using Lorke’s method. The Albino rats were divided into 8 groups of 4 rats each, 6 groups for the fractions and 2 for negative control and positive controls. Alloxan monohydrate (120 mg/kg) was used to induce diabetes. Treatment were with oral doses of the fractions (100 and 300mg/kg body weight) and Glibenclamide 5mg for 7days and their glucose levels were checked daily. The macroscopic and microscopic analysis showed dark green leaf, reticulate veination, smooth texture, fresh leafy odour, glandular trichome, wavy epidermal cell and anisocytic stomata. Analytical evaluation produced total ash (7.00), acid insoluble ash (0.93), water soluble ash (2.96), moisture content (8.40), alcohol extractive value (29.67), water extractive value (40.17) and ethyl acetate extractive value (21.00). The phytochemical analysis revealed flavonoids, alkaloids, steroids, glycosides and saponins are present. The anti-diabetic test revealed a significant decrease in the rat's fasting blood glucose especially in the aqueous methanol fraction (300 mg/kg). The least reduction was seen in chloroform fraction (100 mg/kg).The phytochemical constituents C.barteri has good anti-diabetic effects and can be used for further studies.

Pharmacognostic and physicochemical analysis of the leaves of Fadogia andersonii Robyns (Rubiaceae)

Fadogia andersonii has long been used in Africa in treatment of diseases including inflammation, arthritis, malaria and as aphrodisiac. The use and demand of this medicinal plants has been growing over long period of time. This study was carried out to determine some important pharmacognostic parameters of F. andersonii leaf. Evaluation of the fresh, powdered leaves was carried out to determine the macro-morphological, qualitative and quantitative microscopic, chemomicroscopic features, physicochemical properties and elemental analysis of the leaves. The leaves are pinnate opposite arranged, oblique lamina, glabrous appearance, elliptical in shape, entire margin with reticulate venation. The microscopy revealed straight polygonal wall of epidermal cells, paracytic stomata, unicellular trichomes, xylem, phloem, prismatic & druse crystals, and starch grains. Chemomicroscopy revealed presence of cellulose, cutin, lignin, tannins, starch, calcium oxalate crystals and gum & mucilage. The physicochemical parameters for the leaves estimated include moisture contents (13.11%), total ash value (5.67%), water soluble ash (1.33%), acid insoluble ash (2.00%), water extractive values (13.33%) and alcohol extractive values (10.67%). Elemental analysis revealed the presence of iron, copper, manganese, zinc, nickel, and lead. The pharmacognostic standard observed in this study will be of help in correct identification and quality control of F. andersonii.

Phytochemical and antioxidant studies of Hibiscus Cannabinus seed oil

Abstract Hibiscus cannabinus is used in traditional medicine for treatment of cancer, bacterial infections, diabetics and other diseases. The study was carried out to investigate the phytochemical and antioxidant properties of the oil extracted from Hibiscus cannabinus. The physicochemical parameters of the seed powder were determined using standard procedures. The oil was extracted using Soxhlet extractor and the phytochemical composition was analysed using qualitative tests and GC-MS. The organoleptic characters of the oil were evaluated using standard protocols. Oral acute toxicity in Wistar rats was assayed using the method outlined by the Organization for Economic Co-operation and Development (OECD 425). Qualitative and quantitative antioxidant activity were determined using DPPH free radical assay. The result of the physicochemical evaluation revealed moisture content (8.0%), total ash (4.0%), acid insoluble ash (5.0%), alcohol extractive value (5.5%) and water extractive value (4.5%). The percentage yield of the oil was 13.2%. The organoleptic evaluation of the oil revealed characteristic taste, yellowish colour, characteristic smell and smooth texture. The oil had specific gravity of 0.94 and was found to be soluble/miscible in some organic solvents. The preliminary phytochemical screening revealed the presence of steroids/triterpenoids. The GC-MS revealed the presence of some fatty acids and their esters. The acid value (65.64 mgKOH/g of oil), saponification value (159.32 mgKOH/g of oil), ester value (93.68 mgKOH/g of oil) and iodine value (11.40 gI2/100 g of oil) were obtained. The oral acute toxicity showed that the oil was not toxic up to a dose of 5000 mg/kg per body weight. The oil demonstrated free radical scavenging activity with highest percentage inhibition of 85.30% at 0.441 μg/mL. The results of the research showed that Hibiscus cannabinus oil contain antioxidant compounds and the oil is not toxic when tested in rats. Therefore, the oil can be used in food and pharmaceutical industries.

Pharmacognostic evaluation and coagulant analysis of the ethanol extracts of the leaves of Ocimum gratissimum L. (Lamiaceae)

Ocimum gratissimum belongs to the group of plants known as spices. The plant is an erect small plumb with many branches usually not more than 1m high. It is of the familylabiatae, genus Ocimum and species gratissimum. It is commonly known as scent leaf. This project is focused on the pharmacognostic and coagulant activities of the ethanol extract of the leaves of Ocimum gratissimum. The ethanol leaf extract of the plant was obtained using cold maceration with ethanol and then filtered and concentrated using rotary evaporator. The extract was used for coagulant evaluations, pharmacognostic evaluation and chromatographic (thin layer chromatography) analysis. The LD50 was determined in rats using Lorkes method. Microscopic result shows the presence of a covering or uniseratetrichomes, sclerenchyma none pitted with lumen and diacytic stomata. The numerical data obtained were 8.75%, 12% and 8.45% for moisture content, alcohol extractive value and total cash value. The LD50 shows that there was no death recoded because of the holistic use of the plants. The result of the coagulation research are as follow: Group 1 clotting time 1: 69 min and bleeding time 2: 62min, group 2 clotting time 1: 39 min and bleeding time 4: 79min, group 3 clotting time 1: 37 min and bleeding time 1: 99min, group 4 clotting time 1: 81mins and bleeding time is 2: 28mins, group 5 clotting time is 1: 47mins and bleeding time is 2: 49mins) This shows that the ethanol extract of Ocimum gratissimumon rats exhibited coagulant property. The phytochemical analysis of this plant reveals that it contains alkaloids, flavonoids, saponins, terpenoids, tannins, glycosides and resins. It can therefore be concluded that the ethanol extract of Ocimum gratissimum possesses coagulant activity. This study can be of great importance to the development of new coagulant drugs. Citation: Osualaet al.(2021) Pharmacognostic Evaluation and Coagulant screening of Ethanol Extract of the Leaves ofOcimumgratissimumL. (Lamiaceae) Journal

Microscopic, Physicochemical and Phytochemical Evaluation of Stem bark of Aubrevillea kerstingii (Harms) Pellegr. (Mimosaceae)

Microscopic, physicochemical and phytochemical evaluation gives detailed valuable information that is useful in determination of the identity, quality and purity of medicinal plants, crude drugs and herbal products. The research was carried out in order to determine the microscopic, physicochemical and phytochemical parameters of stem bark extract of Aubrevillea kerstingii. The macroscopic, microscopic, chemo-microscopic and physicochemical and preliminary phytochemical screening parameters were determined according to standard methods.The microscopic features revealed the presence of thin walled, radially arranged cork cells, well developed cortex, bundles of sclerenchymatous cells and numerous prismatic calcium oxalate crystals The chemo-microscopy of the powdered stem bark revealed the presence of some cell wall materials and cell inclusions. Physicochemical parameters determined in this study showed moisture content to be 8.2 ± 0.07, total ash value 9.23 ± 0.56, acid insoluble ash 1.49 ± 0.12, water soluble ash 5.52 ± 0.10, alcohol extractive value 26.50 ± 0.61 and water extractive value 33.4 ± 0.36. Preliminary phytochemical screening revealed the presence of saponins, triterpenes, flavonoids, tannins, alkaloids and carbohydrate. The parameters of stem bark of Aubrevillea kerstingii determined in this study will help in establishing the quality and purity of the plant.
 Keywords: Aubrevillea kerstingii, Fabaceae, Physicochemical, Phytochemical

Comparative shelf-life study of Raw Guggulu (Commiphora wightii oleo-gum resin) and Shodhita Guggulu (cow urine processed C. wightii oleo-gum resin)

BACKGROUND: Shelf-life is an important aspect of raw as well as finished drugs. Recently, shelf-life parameters have been included in the Ayurvedic Pharmacopeia of India. The objective of this article is to evaluate and compare the long-term shelf-life study of Raw Guggulu (RG) (C. wightii oleo-gum resin) and Shodhita Guggulu (SG) (cow urine processed C. wightii oleo-gum resin). MATERIALS AND METHODS: A stability chamber with environmental conditions 30°C ± 2°C/60% ± 5% RH was used for long-term shelf-life study of RG and SG. Physico-chemical parameters such as loss on drying, pH, total ash, acid-insoluble ash, water extractive value, and alcohol extractive value were tested at regular frequency (0th, 3rd, 6th, 9th, and 12th month). RESULTS: Significant changes have been observed w.r.t. analysis of physico-chemical parameters. Physico-chemical parameters revealed that the shelf-life of RG ranged from 42 to 66 months and shelf-life of SG ranged from 39 to 62 months. CONCLUSION: The average shelf-life of RG and SG was found out to be 55.16 and 48.16 months, respectively. These studies may help to understand the shelf-life of various ayurvedic formulations of RG and SG.

Quality Assessment of Selected Anti-Asthmatic Herbal Products Marketed in Kaduna State Metropolis, Nigeria

Background: Herbal medicines are the oldest form of healthcare known to humanity and it is used worldwide for prevention and treatment of different disease conditions. The presence and proliferation of substandard herbal medicines in the market that do not fulfil standards set by the regulating bodies in Nigerian is a matter of concern. Objective: To assess quality of some anti-asthmatic herbal products marketed in Kaduna State using standard methods. Method: Ten (10) different samples of anti-asthmatic herbal products marketed in Kaduna metropolis were selected randomly and standard methods by World Health Organization methods, Trease and Evans, and digestion by AOAC were used to assess the quality of the herbal samples. Results: The result obtained showed that 50 % of the herbal preparations contain moisture content higher than the permissible limit (8 %), while 40 and 10 % of the samples had water extractive and alcohol extractive values respectively lower than the 15 % minimum limit stipulated by European Pharmacopeia. Also, 30 % of the samples had higher ash values than the 14 % maximum acceptable limit recommended by European Pharmacopoeia. All the herbal preparations analyzed contain cardiac glycosides, carbohydrates, alkaloids, steroids and flavonoid, while 80 % of the samples contain terpenoids, 70 % of the samples contain saponins and 50 % contain tannins. Also, 80 and 50 % of the samples analyzed contained cadmium and lead respectively above WHO permissible limits (0.3 and 10 ppm respectively). Conclusion: None of the anti-asthmatic herbal products analyzed passed the quality assessment carried out in this study.

Zarawand Mudharaj (Aristolochia rotunda Linn.), an important medicinal plant used in Unani system of medicine: A review

Background: Zarawand Mudharaj (Aristolochia rotunda L.) belongs to family Aristolochiaceae. According to Unani scholars, it is a female plant of Aristolochia fontanesii Boiss. & Reut. (Syn. A. longa L.), usually found growing in Mediterranean regions, Central Asia and South Europe.
 Purpose of the review: The main aim of this review is to highlight various aspects of Zarawand Mudharaj such as temperament, botanical description, pharmacological properties, therapeutic uses etc, as mentioned in Unani literature that will ultimately guide researchers to design various studies for further exploration of this important medicinal plant.
 Materials and methods: The present review has been carried out through extensive literature survey of various classical Unani and botanical texts, and published papers available on different search engines. The botanical names and their synonyms were validated through ‘The Plant List’ (www.theplantlist.org).
 Results: In Unani medicine, the rhizome of Zarawand Mudharaj is used for the treatment of several ailments viz. stomach and liver diseases, jaundice, cough, septic wounds, splenomegaly, gout etc. Apart from its use as single drug, it has also been added in many compound preparations viz. Anqarooya-i-Kabir, Habb-i-Rewand, Majun Falasifa, Majun Dabeedul Ward etc. A study on physicochemical standardization of rhizome of Aristolochia rotunda L. has reported the presence of moisture content, total ash, acid insoluble ash, water soluble ash, sulphated ash, alcohol and water-soluble extractive values within normal limits. The same study has also revealed that the hydro-alcoholic extract of rhizome of Aristolochia rotunda L. possesses significant hepatoprotective activity against CCl4 induced liver toxicity in albino Wistar rats. Several toxicity reports have pointed out that aristolochic acid containing herbal drugs including Aristolochia rotunda L. cause renal impairment and other serious adverse reactions. 
 Conclusion: It is concluded that Aristolochia rotunda L. is widely used in Unani system of medicine. However, extensive studies on pharmacognosy, pharmacology, toxicology, phytochemistry and quality control of different parts of this potential medicinal plant have not been carried out so far. Therefore, such studies on modern scientific parameters may be conducted for further exploration so that the benefits may reach the masses.
 Keywords: Zarawand Mudharaj; Aristolochia rotunda L.; Unani medicine; Hepatoprotective; Aristolochic acid; Toxicity

Physicochemical, Phytochemical and Toxicological Study of Hydroalcoholic Extract of Aerva javanica Roots

Aim: The study is aimed at determining the characters of roots of Aerva javanica (A.javanica) assessing acute oral toxicity of hydroalcoholic extract of roots of Aerva javanica.
 Place and Duration of Study: the physicochemical and phytochemical evaluation was carried out at Faculty of Pharmacy, Maulana Azad University Jodhpur, Rajasthan. Acute Oral Toxicity was studied at Bilwal Medchem and Research Laboratory, Jaipur Rajasthan. The duration of study June 2021 – July 2021
 Methodology: The pharmacognostical characters were evaluated in terms of organoleptic property, physico-chemical parameters, and preliminary phytochemical investigation. The acute oral toxicity was determined using the 423, OECD guideline for testing of chemical, acute toxic class method.
 Results: The physico-chemical analysis revealed total ash; water soluble ash and, acid insoluble ash to be 6.36 ± 0.26%, 0.79 ±0.23 % and, 1.23 ± 0.34%. The water, alcohol and petroleum ether soluble extractive values were found to be 17.88± 3.54 %, 15.58 ± 1.13% and, 0.3 ± 0.13 %. The percentage yield of hydroalcoholic extract of root of A.javanica was found to be 24%. The phytochemical screening of hydroalcoholic extract revealed the presence of carbohydrates, flavonoids, steroids, alkaloids, tannins, proteins, and fixed oil. The acute oral toxicity of hydroalcoholic extract of root of A.javanica revealed that the extract was found to be safe till 2000 mg/kg BW.
 Conclusion: The results of the present study will furnish data helpful in the correct identification and authentication of roots of A.javanica. The extractive value shed light on the most suitable solvent to be chosen to obtain extract rich in phytoconstituents. The physicochemical screening furnished data on important phytoconstituents present in the hydroalcoholic extract which could be helpful in isolation and purification of desired phytoconstituents. Acute oral toxicity study revealed that the extract is safe till 2000 mg/kg BW which could be helpful in selection of dose for future pharmacological activities.

Formulation, Evaluation and Comparison of the Poly Herbal Anti-Diabetic Tablet with the Commercial Tablets

Objective: To formulate a poly herbal anti-diabetic tablet and to evaluate and compare its physicochemical properties with the marketed herbal tablets.
 Materials and Methods: The poly herbal anti-diabetic tablet was formulated by adding the powder of extract of Enicostemma littorale in powder of roots of Aconitum heterophyllum rhizomes of Picrorhiza kurroa and fruits of Piper longum in different proportions to an aqueous 5 % Starch solution and Several tests such as visual inspection, ash values, moisture content, Water soluble extractive value and Alcohol soluble extractive value, disintegration time, Uniformity of weight of tablets, Determination of hardness of tablets, Determination of friability of tablets etc and Preliminary phytochemical screening and qualitative chemical examination were performed and compare Laboratory formulated tablets with commercial tablets.
 Results: The study showed that Laboratory formulated poly herbal anti-diabetic tablet has good flow property and compressibility. The moisture content of laboratory formulations was found to be 4.8%. Water extractive value was found to be 28.14% W/V result shows that laboratory formulation consisted of higher amount of water soluble substances like carbohydrates. Alcohol extractive value was found to be 20.08% W/V denotes the amount of alcohol soluble constituents present in the formulation. Disintegration of tablet was found to be 3 mins results shows laboratory formulation was within the limit as it was prepared with starch paste (5%w/v) as a binding and disintegrating agent. Disintegration of tablet is not more than 30 minutes. The laboratory formulation was found to have 3 (Kg/Cm2) hardness, 0.38 % Friability and weight variation within pharmaceutical limits. However, further research and development is required to improve its quality and safety.
 Conclusion: The aim of this study was to formulate a completely poly herbal antidiabetic tablet. Our Laboratory formulated tablets showed comparable good results as compare to that of marketed tablets but further research and development is required to improve its overall quality.

Bioactive chemical constituents, acute toxicity and 1,1-diphenyl-2-picrylhydrazyl radical scavenging activity of Polyalthia longifolia root

Polyalthia longifolia (masquerade tree) is a plant which is believed to possess varied pharmacological and therapeutic values among different populations. The present report investigated the phytochemical composition, proximate, acute toxicity and antioxidant potential of P. longfolia root. All analyses were carried out using established methods; the antioxidant activity of the crude methanol extract and its fractions (n-hexane and ethyl acetate) were examined using 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging assay while the total phenolic and flavonoid contents were assessed using the Folin-Ciocalteu and the aluminum chloride calibration methods respectively. The phytochemical analysis revealed the presence of alkaloids, carbohydrate, reducing sugars, tannins, saponins, flavonoids, phenolic compounds and protein in aqueous extract. The proximate analysis showed moisture content, total ash, alcohol extractive value, water extractive value, acid insoluble ash and water soluble ash at 8.80, 9.35, 3.28, 3.29, 2.27 and 7.29% respectively. The ethyl acetate fraction showed the highest antioxidant property compared to the n-hexane fraction and crude methanol extract in all assays conducted. Also, the methanol fraction was found to have the highest flavonoids and phenolic content among the extract and fractions. Oral administration of crude methanol extract of P. longifolia to Swiss mice was relatively non-toxic at a maximum dose of 5000 mg/kg. The root extract and fractions of P. longifolia indicated moderately high level of some phytochemicals with outstanding radical scavenging activity, and therefore substantiate its use as a conventional and comparatively non-toxic plant antioxidant.

EFFECT OF INDUSTRY EFFLUENT ON HISTO-PHARMACOGNOSY OF BOERHAAVIA DIFFUSA LINN. USED AS BLOOD PURIFIER

Aims of the Study: To carried out the effect of Atlas Cycles Industry effluent on pharmacognosy of Boerhaavia diffusa Linn. used as Blood Purifier Method: The effluent of Atlas Cycles Industry was analyzed by Trivedi & Goel, 1986 method. Metacalf and Chalk, 1950 was consulted for anatomical studies of selected plant; for chemical analysis Johanson, 1940, Cromwell, 1955 & Trease and Evans, 1983 were followed. TLC was investigated by WHO, 1998. Results: The physico-chemical parameters of Atlas cycle industry effluent were found greater values as compared to standard values. The morphological & anatomical parameters were showed decreasing trend in those plants which were collected near the vicinity of Atlas cycle industry. The colour reaction tests resulted only degrees of changes. The number of spots in observation of TLC, stomatal index, palisade ratio, water extractive and alcohol extractive values were reduced in those plants which were collected near the vicinity of Atlas cycle industry where as vein Islet & vein termination number, ash values were comparatively higher in same samples. Conclusion: The conclusion of this study is that the plants should not be collected form polluted areas(near the vicinity of any industry) for the preparation of medicines, since majority of parameters reflect decreasing data in those plants which were taken from polluted area.