Albumin Fraction Research Articles

Biological value of zaanen goats' pasteurized milk proteins

Goat's milk is positioned as biologically complete, suitable for the creation of functional products and baby food products. This is explained by the qualitative and quantitative composition of its main nutrients: proteins, lipids, carbohydrates, biologically active substances, micro- and macro-elements. The biological value of food raw materials is assessed by its ability to satisfy protein needs. Strict thermal treatment regimens are used for goat milk. This is caused by the quantitative ratio of casein and albumin fractions in goat milk and its specific organoleptic properties. Pasteurization of milk causes partial destruction of proteins, enzymes, hormones and evaporation of gaseous. This contributes to the improvement of sensory properties of dairy goat raw materials. The effect of high temperatures on goat milk proteins and its biological value has been little studied. The effect of pasteurization regimes on proteins was studied and indicators of the biological value of the samples were determined: without heat treatment; heat treatment 63 ± 2 °С, duration 30 minutes; heat treatment 87 ± 2 °С duration 5-6 minutes. The amino acid composition of the test samples was determined by acid hydrolysis on an LC2000 amino acid analyzer (Biotronik, Germany). Indicators of the biological value of proteins of pasteurized goat milk were calculated – coefficient of difference of amino acid composition, biological value, coefficient of utilitarian amino acid composition, coefficient of rationality of amino acid composition, coefficient of comparative redundancy. The general analysis of the obtained data revealed a positive effect of heat treatment on the indicators of the biological value of goat milk. The amino acid score of the limiting amino acid increased by 14.82–14.92 %; biological value – 11.4–13.02 %; the PDCAAS indicator – by 14.18–14.29 %; changes in the values of formalized indicators had the same tendency. The biological value of proteins for the application of thermal regimes is at the same level. It has been proven that pasteurization has a positive effect on the biological value of goat milk proteins. This makes the product safe and useful for all segments of the population and can be recommended for feeding children from 0 to 6 months. Prospects for further research are the development of milk drinks of a combined composition of raw materials with an improved recipe in order to enrich the product with limiting amino acids.

SDS Electrophoresis on Gradient Polyacrylamide Gels as a Semiquantitative Tool for the Evaluation of Proteinuria.

Proteinuria is an important sign of kidney diseases. Different protein patterns in urine associated with glomerular, tubular and overload proteinuria may be differentiated using the immunochemical detection of indicator proteins or via urinary proteins electrophoresis. Our aim was to characterize sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) using commercially available 4-20% gradient gels as a method to detect and differentiate proteinuria. Our laboratory-based study used excess urine samples collected for routine diagnostic purposes from adult patients of a tertiary-care hospital, including patients with albumin/creatinine < 30 mg/g and patients with dipstick proteinuria. The limit of albumin detection was estimated to be 3 mg/L. In 93 samples with albumin/creatinine < 30 mg/g, an albumin fraction was detected in 87% of samples with a minimum albumin concentration of 2.11 mg/L. The separation of 300 urine samples of patients with proteinuria revealed distinct protein patterns differentiated using the molecular weights of the detected proteins: glomerular (albumin and higher molecular weights) and two types of tubular proteinuria ("upper" ≥20 kDa and "lower" with lower molecular weights). These patterns were associated with different values of the glomerular filtration rate (median 66, 71 and 31 mL/min/1.72 m2, respectively, p = 0.004) and different proportions of multiple myeloma and nephrological diagnoses. As confirmed using tandem mass spectrometry and western blot, the SDS-PAGE protein fractions contained indicator proteins including immunoglobulin G, transferrin (glomerular proteinuria), α1-microglobulin, retinol-binding protein, neutrophil gelatinase-associated lipocalin, cystatin C, and β2-microglobulin (tubular), immunoglobulin light chain, myoglobin, and lysozyme (overflow). SDS-PAGE separation of urine proteins on commercially available 4-20% gradient gels is a reliable technique to diagnose proteinuria and differentiate between its main clinically relevant types.

Elucidation of the structure, antioxidant, and interfacial properties of flaxseed proteins tailored by microwave treatment

The microwave treatment is commonly applied to flaxseed to release nutrients, inactivate enzymes, remove cyanogens, and intensify flavors. The current study aimed to explore the influences of microwave exposure on the antioxidant and interfacial properties of flaxseed protein isolates (FPI), focusing on the altering composition and molecular structure. The results showed that after microwave exposure (700 W, 1–5 min), more compact assembly of storage proteins and subsequent permeation by membrane fragments of oil bodies occurred for cold-pressing flaxseed flours. Moreover, the particle sizes of FPI was progressively reduced with the decrement ranged from 37.84 to 60.66% (P<0.05), whereas the zeta potential values initially decreased and then substantially recovered during 1–5 min of microwave exposure. The conformation unfolding, chain cross-linking, and depolymerization were sequentially induced for FPI based on the analysis of fluorescence emission spectra, secondary structure, and protein subunit profiles, thereby affecting the dispersion or aggregation properties between albumin and globulin fractions in FPI. Microwave exposure retained specific phenolic acids and superior in vitro antioxidant activities of FPI. The inferior gas–water interface absorption and the loose/porous assembly structure were observed for the foams prepared by FPI, concurrent with obviously shrinking foaming properties upon microwave exposure. Improving oil–water interface activities of FPI produced the emulsion droplets with descending sizes and dense interface coating, which were then mildly destabilized due to the lipid leakage and weakened rheological behavior with microwave exposure extended to 5 min. Our findings elucidated that microwave treatment could tailor the application functionality of protein fractions in flaxseed based on their in situ structural remodeling.

Differences and similarities of albumins from japonica rice bran, japonica, indica, and javanica rice endosperm in the suppressive effect of postprandial blood glucose elevation

AbstractSuppression of rapid increase in blood glucose level using food components can prevent diabetes. To use functional food components from natural resources, information regarding the plant varieties and plant parts where the component exists is important for the effective extraction and the effective use of biological resources. We have previously found that proteins from the albumin fraction of rice endosperm are indigestible and adsorb glucose to suppress postprandial blood glucose elevation in vivo. However, the variety and location of this functional protein remain unknown. We investigated whether water‐soluble fraction from the endosperm of indica and javanica rice and red and middle bran of japonica rice contains functional albumin observed in the endosperm of japonica. Middle bran was found to possess an indigestible albumin with the same molecular weight as that in the endosperm of japonica and adsorb a large amount of glucose. Middle‐bran albumin suppressed postprandial blood glucose elevation after oral glucose loading in animal experiments, as well as the rice‐endosperm albumin. As middle bran is the outer layer of the endosperm, many functional components are distributed. Our study can aid the effective use of middle bran, which is predominantly discarded in the process of making rice wine, as a functional food material for the prevention of diabetes mellitus.

CLINICAL PROFILE AND SHORT TERM OUTCOME OF CHILDREN WITH COVID-19 RELATED MULTISYSTEM INFLAMMATORY SYNDROME (MISC) TREATED WITH PULSE METHYLPREDNISOLONE AND/OR IV IMMUNOGLOBULIN : A CASE SERIES FROM TERTIARY CARE PAEDIATRIC HOSPITAL IN BANGALORE

Introduction: MIS-C in children is a rare, but life threatening complication of covid 19 infection. The complications of MIS-C can be severe, including cardiogenic shock or distributive shock with poor vasomotor tone. Aim: To study the clinical prole and outcome of children treated with methylprednisolone pulse therapy and/or IV immunoglobulin. Materials and methods: Case series of children diagnosed with COVID 19 related MISC admitted to Vydehi Institute of Medical Sciences and Research Centre, Bangalore from march to august 2021 , who met the WHO and CDC criteria were reviewed. Results: The case series describes 11 cases of MISC in our hospital. 3 children were treated with IV immunoglobulin and 8 children were received IV pulse methylprednisolone. All children had fever upon arrival to hospital lasting for atleast 5 days. Children with shock also had higher D-dimer , lower albumin , higher CRP, high lactate and lower ejection fraction compared to other children. Conclusion: We have concluded a favourable role of IV immunoglobulin or pulse methylprednisolone in treatment of MISC.

Relationship between serum CHIP levels with cardiovascular disease in maintenance haemodialysis patients.

Many preclinical studies reported that the carboxyl terminus of Hsp70-interacting protein (CHIP) has cardiovascular protective effects. This study was designed to explore whether CHIP is related with cardiovascular disease (CVD) in maintanence haemodialysis (MHD) patients. 217 MHD patients and 150 healthy controls were recruited, serum CHIP concentration and clinical characteristics were measured. MHD patients were followed-up for 36 months and their cardiovascular events (CVEs) and survival conditions were recorded. Here, the data shows that serum CHIP concentrations in MHD patients were lower than those in healthy controls (31.69 ± 18.2 pg/mL vs 84.53 ± 22.1 pg/mL, p < 0.05). CHIP negatively correlated with age, C-reactive protein, B-type brain natriuretic peptide, phosphorus, parathyroid hormone, carotid intima-media thickness (CIMT) and left ventricular septal thickness (LVSTd), whereas it positively associated with albumin, haemoglobin, creatinine, Kt/V and ejection fraction (p < 0.05, respectively). Partial correlation and multiple linear regression analysis verified the negative relationship between CHIP with CIMT or LVSTd (p < 0.05, respectively). Using quartile method and Kaplan-Meier survival function, it indetified that the lower serum CHIP concentration predicted risk of CVEs, CVD and all-cause death (p < 0.001). Cox regression analysis manifested CHIP was negatively associated with CVEs (HR=0.914, 95%CI 0.880-0.950, p < 0.001), CVD mortality (HR=0.747, 95%CI 0.651-0.857, p < 0.001) and all-cause death (HR=0.769, 95%CI 0.696-0.850, p < 0.001). In conclusion, the data of this study revealed that serum CHIP level is significantly correlated with multiple risk factors of CVD and may be one of the predictors of CVD risk and death in MHD patients.

Modification of the structural stability of human serum albumin in rheumatoid arthritis.

Differential scanning calorimetry (DSC) can indicate changes in structure and/or concentration of the most abundant proteins in a biological sample via heat denaturation curves (HDCs). In blood serum for example, HDC changes result from either concentration changes or altered thermal stabilities for 7-10 proteins and has previously been shown capable of differentiating between sick and healthy human subjects. Here, we compare HDCs and proteomic profiles of 50 patients experiencing joint-inflammatory symptoms, 27 of which were clinically diagnosed with rheumatoid arthritis (RA). The HDC of all 50 subjects appeared significantly different from expected healthy curves, but comparison of additional differences between the RA and the non-RA subjects allowed more specific understanding of RA samples. We used mass spectrometry (MS) to investigate the reasons behind the additional HDC changes observed in RA patients. The HDC differences do not appear to be directly related to differences in the concentrations of abundant serum proteins. Rather, the differences can be attributed to modified thermal stability of some fraction of the human serum albumin (HSA) proteins in the sample. By quantifying differences in the frequency of artificially induced post translational modifications (PTMs), we found that HSA in RA subjects had a much lower surface accessibility, indicating potential ligand or protein binding partners in certain regions that could explain the shift in HSA melting temperature in the RA HDCs. Several low abundance proteins were found to have significant changes in concentration in RA subjects and could be involved in or related to binding of HSA. Certain amino acid sites clusters were found to be less accessible in RA subjects, suggesting changes in HSA structure that may be related to changes in protein-protein interactions. These results all support a change in behavior of HSA which may give insight into mechanisms of RA pathology.

The effect of a high-calorie diet on the total content of chemical elements and metal-ligand forms of zinc in the blood serum and liver of Wistar rats

Background. Worldwide, there is a rapid increase in the number of people suffering from various forms of carbohydrate and lipid metabolism disorders. Modern studies show that the transport, distribution, excretion and accumulation of chemical elements in these types of metabolic disorders change in different ways and affect the further functional state of the body differently.The aim. To evaluate the level of macro- and microelements in the blood serum and liver, as well as the content of metal-ligand forms of zinc in the blood serum of a Wistar rat in a high-calorie diet.Materials and methods. Thirty male rats were selected for the experiment, from which two groups were formed: control (n = 15) and experimental (n = 15). The animals of the control group received the basic diet (270 kcal/100 g), and the animals of the experimental group received a high-calorie diet. During the experiment, the caloric content of the diet of the experimental group gradually increased from the caloric content of the total diet. During the study, body weight, biochemical parameters of blood and urine were evaluated. The analysis of macro- and microelements in the samples was carried out using inductively coupled plasma mass spectrometry. Determination of the content of individual zinc compounds in blood serum was carried out using a combination of a chromatograph and a mass spectrometer.Results. It was found that a high-calorie diet led to a decrease in the level of iron, chromium, iodine, zinc, potassium, calcium, and an increase in vanadium in blood serum. In the liver, there was a decrease in the level of lithium and an increase in the level of calcium, vanadium, chromium, iron, zinc, cobalt. When assessing the chemical forms of zinc in the blood serum, a percentage increase in the albumin fraction was recorded against the background of a decrease in amino acid complexes and low-molecular-weight forms of zinc.Conclusion. The data obtained suggest that a high-calorie diet leads to an imbalance of chemical elements, which can serve as one of the triggers for dysregulation of a number of physiological functions of the body.

Development and validation of a nomogram to predict mortality risk in patients with ischemic heart disease.

Ischemic Heart Disease (IHD) is the leading cause of death from cardiovascular disease. Currently, most studies have focused on factors influencing IDH or mortality risk, while few predictive models have been used for mortality risk in IHD patients. In this study, we constructed an effective nomogram prediction model to predict the risk of death in IHD patients by machine learning. We conducted a retrospective study of 1,663 patients with IHD. The data were divided into training and validation sets in a 3:1 ratio. The least absolute shrinkage and selection operator (LASSO) regression method was used to screen the variables to test the accuracy of the risk prediction model. Data from the training and validation sets were used to calculate receiver operating characteristic (ROC) curves, C-index, calibration plots, and dynamic component analysis (DCA), respectively. Using LASSO regression, we selected six representative features, age, uric acid, serum total bilirubin, albumin, alkaline phosphatase, and left ventricular ejection fraction, from 31 variables to predict the risk of death at 1, 3, and 5 years in patients with IHD, and constructed the nomogram model. In the reliability of the validated model, the C-index at 1, 3, and 5 years was 0.705 (0.658-0.751), 0.705 (0.671-0.739), and 0.694 (0.656-0.733) for the training set, respectively; the C-index at 1, 3, and 5 years based on the validation set was 0.720 (0.654-0.786), 0.708 (0.650-0.765), and 0.683 (0.613-0.754), respectively. Both the calibration plot and the DCA curve are well-behaved. Age, uric acid, total serum bilirubin, serum albumin, alkaline phosphatase, and left ventricular ejection fraction were significantly associated with the risk of death in patients with IHD. We constructed a simple nomogram model to predict the risk of death at 1, 3, and 5 years for patients with IHD. Clinicians can use this simple model to assess the prognosis of patients at the time of admission to make better clinical decisions in tertiary prevention of the disease.

Interaction of Positively Charged Oligopeptides with Blood Plasma Proteins.

In this project, we combine two areas of research, experimental characterization and molecular docking studies of the interaction of positively charged oligopeptides with crucial blood plasma proteins. The investigated peptides are rich in NH2 groups of amino acid side chains from Dap, Orn, Lys, and Arg residues, which are relevant in protein interaction. The peptides are 9- and 11-mer with the following sequences: (Lys-Dab-Dab-Gly-Orn-Pro-His-Lys-Arg-Lys-Dbt), (Lys-Dab-Ala-Gly-Orn-Pro-His-Lys-Arg), and (Lys-Dab-Dab-Gly-Orn-Pro-Phe(2-F)-Lys-Arg). The net charge of the compound strongly depends on the pH environment and it is an important aspect of protein binding. The studied oligopeptides exhibit therapeutic properties: anti-inflammatory activity and the capacity to diminish reactive oxygen species (ROS). Therefore, the mechanism of potential binding with blood plasma components is the next challenge. The binding interaction has been investigated under pseudo-physiological conditions with the main blood plasma proteins: albumin (BSA), α1-acid glycoprotein (AAG), and γ-globulin fraction (GGF). The biomolecular quenching constant (kq) and binding constant (Kb) were obtained by fluorescence spectroscopy at various temperatures. Simultaneously, the changes in the secondary structure of proteins were monitored by circular dichroism (CD) and infrared spectroscopy (IR) by quantity analysis. Moreover, molecular docking studies were conducted to estimate the binding affinity, the binding domain, and the chemical nature of these interactions. The results show that the investigated oligopeptides could be mainly transported by albumin, and the binding domain I is the most favored cavity. The BSA and GGF are able to form stable complexes with the studied compounds as opposed to AAG. The binding reactions are spontaneous processes. The highest binding constants were determined for Lys-Dab-Dab-Gly-Orn-Pro-His-Lys-Arg-Lys-Dbt peptide, in which the values of the binding constants Kb to BSA and GGF were 10.1 × 104 dm3mol-1 and 3.39 × 103 dm3mol-1, respectively. The positively charged surface of peptides participated in salt bridge interaction with proteins; however, hydrogen bonds were also formed. The secondary structure of BSA and GGF after contact with peptides was changed. A reduction in the α-helix structure was observed with an increase in the β-sheet and β-turn and random coil structures.

Comparative analysis of the efficiency of seed protein profiles in assessing genetic variation and population structure among indigenous Manipur black rice cultivars.

The state of Manipur, North East India has distinct topology of hill and valley regions with vast agroclimatic variability, being considered as one of the centers of rice diversity. The indigenous Manipur black rice cultivars exhibit wide range of diversity in morphology, pericarp color, shape and size of grain, aroma, glutinous or non-glutinous features but remain less characterised. Many of these cultivars, such as those named Chakhao, are endowed with multiple health benefits due to high anthocyanins, and hold special importance for the local people. It is important to analyse the genetic diversity and population structure for this germplasm with unique allelic combinations to utilize in rice breeding programme. We characterized total soluble seed protein fractions to not only fingerprint the 45 indigenous black rice cultivars but assess their genetic relatedness. Cluster analyses generated mainly two groups, complemented by PCoA scatter plot ascertaining geographical distinction. The hill black rice were more diverse. The population structure analysis revealed seven subpopulations indicating high genetic variability. The 24 polymorphic bands were scored in the range of 127.8 to 10.3kDa comprised of four protein fractions. Three polypeptide bands each were ascribed to known fractions of glutelins and prolamins, while one band each could be described for albumin and globulin fractions, besides other diagnostic bands. Some diverse cultivars were Amubi, Chedo Anal, Chipi Buh, Athebu, Poireton, BuPu Mui, Kotha Chahao II. These cultivars can be used in future black rice breeding programmes. This can further prevent genetic erosion and protect intellectual property rights.

Research of romanov sheep metabolism characteristics over the period of lactation in estations of North-Western region

Selenium is an essential trace element in the diet of farm animals. Selenium is a part of the antioxidant enzymes, preventing the free radicals cell destruction. Selenium availability varies greatly due to the great number of biogeochemical territories. The NorthWestern region of Russia is a territory with selenium deficiency in soils and plants. In this study, it was found that the concentration of selenium in pasture plants was in the range from 0,059 to 0,067 µg /kg with an optimal value of 0,1 mg/kg. It was found that the concentration of hemoglobin, the number of red blood cells, the concentration of total protein and albumins in blood of Romanov sheep over the period of lactation was below the reference values. The number of red blood cells also increased. On the 100th day, the number of red blood cells was higher by 7.78% compared to the 30th day and by 14.59% compared to the 10th day since the beginning of lactation. There was an increase in the concentration of total protein due to the albumin fraction. On the 100th day, the total protein concentration was 15.29% higher compared to the 10th day and 9.46% higher compared to the 30th day since the beginning of lactation. On the 100th day from the start of lactation, the hemoglobin concentration was 19.07% higher compared to the 10th day from the start of lactation and 10.73% higher compared to the 30th day from the start of lactation. It was marked that those parameters had got higher to the low reference limit at the late period of lactation. This tendency could be the effect of metabolic imbalance and selenium deficiency in sheep diet.

Predictors of diseases in cows in the fresh period

Relevance. This work is devoted to the study of prognostic parameters for the development of labor pathology (retention of the placenta), a complication of which is often postpartum endometritis, as well as mastitis in Holstein cows, which will allow us to accurately and timely develop a preventive algorithm of actions.Methods. To solve research problems, animals with different pregnancy periods of the Holstein breed were selected and divided into two groups in the amount of 42 heads: group 1 — cows with a pregnancy period of 5–6 months; group 2 — cows with a pregnancy of 7–8 months. To identify predictors of diseases in the fresh period, the metabolic status of pregnant cows was studied on the basis of clinical and biochemical blood parameters using laboratory methods; the presence or absence of pathologies in these animals was taken into account in the first 10–14 days after calving.Results. Having studied the level of metabolic processes in selected pregnant cows (second and third trimesters), we were able to found that the concentrations of calcium (2.05±0.034 mmol/l), albumin fraction (12.7±2.31%), glucose (2.20±0.299 mmol/l), bilirubin (10.7± 2.94 mmol/l) in animals with a pregnancy of 5–6 months and the values of the protein index (0.17±0.014), phosphorus (2.75±0.215 mmol/l) at 7–8 months of pregnancy are factors predicting the pathology the labor and the postpartum period, namely retention of the placenta, diseases of the mammary gland and endometrium of an inflammatory nature. The most significant was the fact that a decrease in serum calcium and albumin by 18% and 53% in the second and third trimesters of pregnancy increases the likelihood of diseases in newly calved cows by 27–33%.

Effect of lifestyle-based lipid lowering interventions on the relationship between circulating levels of per-and polyfluoroalkyl substances and serum cholesterol

Exposure to certain per-and polyfluoroalkyl substances (PFAS) has been shown to be positively associated with total and/or low-density lipoprotein cholesterol. Examining this association in lipid lowering interventions may provide additional evidence linking PFAS to cardiovascular risk. We examined the relationship of 6 PFAS with cholesterol in a 6-month lifestyle-based intervention. We quantitated PFAS in 350 individuals at baseline and post intervention and examined associations of PFAS with cholesterol before and after intervention. Food frequency questionnaires and GIS analyses were used to investigate PFAS hotspots and possible exposure routes. Cholesterol significantly decreased following intervention and in parallel, PFOS, PFOA, PFHxS, and PFHpA significantly decreased. PFOS was positively correlated with total cholesterol only post-intervention. We observed that PFOS was distributed among both non-albumin and albumin lipoprotein fractions pre-intervention, but entirely in albumin fraction post-intervention. Our results indicate that lipid-lowering via lifestyle modification may impact on circulating levels or distribution of PFAS.

Polymorphism of the glutathioneperoxidase-1 gene (GPX-1 g. 189 T/C) and biochemical parameters of the blood serum of Holstein cattle

The study examined the polymorphism of the glutathione peroxidase-1 gene (GPX-1, g. 189 T/C) effect on the GPx enzyme activity and biochemical parameters in the blood serum of Holstein cows of indigenous and foreign selection. The work was carried out in the Lenin Breeding Farm Integrated Agricultural Production Center (IAPC) and the Mukhametshin Z.Z. Peasant Farm Enterprise (PFE) of the Republic of Tatarstan. DNA genotyping by the locus of the Bos taurus GPX-1-Bsc4 I gene was performed by PCR-RFLP. As a result of genetic typing of cattle, 2 alleles (C and T) and 3 genotypes (CC, TC and TT) were identified. The classification carried out by PIC values in two populations of Holstein cattle showed that there is a moderate polymorphism of the GPX-1 gene. A negative inbreeding coefficient (F) indicates excessive outbreeding in both populations. In both populations, the numerical advantage of heterozygous genotype TC cows is 60.1 and 56.7 % of the total livestock. Indigenous breeding cows, compared with imported cattle had higher: GPx activity, AST, ALT, cholesterol level, total protein and albumin fraction. Analysis of the association between the GPX-1 gene polymorphism and the GPx enzyme level indicates that the activity of this enzyme varies depending on the genotype, and there is also a variation in the biochemical parameters of the Holstein cattle blood serum.

Blood parameters and hormonal status of sled dogs Siberian husky breed before and after racing

The investigation of the physiological characteristics of sled dogs of Siberian husky breed seems relevant, since dogs of this breed recently play an important role for animal husbandry in the Far North in Russia. Blood samples have been taken before the racing and immediately after it. The study of hormones in the blood of Siberian husky sled dogs before and after the racing showed that the testosterone level dropped sharply after the racing by 7.6 times, which can be explained by the significant energy consumption of the dog's body during the racing. At the same time, cortisol as a hormone that protects the body from stress, regulates blood pressure, participates in the metabolism of proteins, fats and carbohydrates, has significantly increased by 3.6 times (P&lt;0.05), which indicated the activation of the body's protective systems during the period of exposure to stress. The content of estradiol had not undergone significant changes. The exertion affected the content of red blood cells their decrease occurred (by 7.3%), a similar dynamics in hemoglobin level was observed. It was found as the result of biochemical blood tests that the creatinine level after racing increased by 4.6 mmol/l. The levels of aspartate aminotransferase and alanine aminotransferase decreased by 31.5 and 30.8 units/l, respectively. The content of lactate dehydrogenase increased by 31.3 units/l. The level glucose in the blood of sled dogs after racing decreased from 5.0 to 4.5 mmol/l, which was quite understandable by the significant energy consumption of dogs during the racing. The decrease in the content of total protein and its albumin fraction during the racing by 6.0 and 2.5 g/l, respectively was found. Moreover, the decrease in albumin level was statistically significant at P&lt;0.05. As the result of the racing the level of creatine phosphokinase increased by 24.3 units /l, and the content of triglycerides decreased by 0.12 mmol/l.

Research on the productivity and interior of fattening bulls

The results of studies of the productivity and interior of young cattle of the Charolais breed fattened in CJSC Firm of Agrocomplex named after N.I. Tkachev, Vyselkovsky district, Krasnodar Territory. With a live weight before slaughter of 560.4 ± 16.7 kg, carcasses with a slaughter yield of 68.0% were distinguished by high rates of meat: the yield of boneless beef for baby food was 81.0%. At the age of 18 months animals had the highest content of total protein in the blood serum. In comparison with 12-month-old, bulls of 18 months old sowed higher live weight values, as well as higher albumin fraction indicators. The same trend was observed for the protein index (Albumin/Globulin - A/G) - an indicator of the state of protein metabolism (0.72 - at 12 months and 1.23 - at 18 months). An increase of 3.8% in the amount of lysozyme in the blood serum of bulls at the age of 18 months was established. compared to 12 months. (p&lt;0.05).

Cardiac Tamponade in Idiopathic Capillary Leak Syndrome: Philippines’ First

Background: Clarkson’s disease, more known as idiopathic systemic capillary leak syndrome (SCLS) is a rare condition characterized by hypotensive episodes due to capillary hyperpermeability, which may be manifested by fluid accumulation in any organ or potential space, causing range of complications: respiratory failure (from pulmonary edema or pleural effusion), mesenteric hypoperfusion (from hepatic congestion and severe intestinal edema), compartment syndrome and rhabdomyolysis, and even shock from hypovolemia or cardiac tamponade. Very few cases of cardiac tamponade have been reported and certainly a serious and life-threatening complication. Case Summary: A 44-year-old female, reported to have intermittent episodes of angioedema aggravated by menstruation and infection, came in due to persistent hypotension. Patient presented with flu-like symptoms and tested positive for COVID – 19 infection. Initial laboratories revealed hemoconcentration, slightly elevated creatinine and hypoalbuminemia. Electrolytes and urinalysis were normal. 2D echocardiography showed large pericardial effusion with right atrial and ventricular collapse suggestive of cardiac tamponade, hence patient underwent emergency pericardial windowing. Initial chest X-ray was normal but repeat study showed pleural effusion on the 4th day of admission which was drained surgically. Additional diagnostic tests revealed negative for hepatitis, Mycoplasmal and Epstein-Barr virus. Direct and indirect Coomb’s test were also negative and Lupus panel was unremarkable. Serum free light chain panel showed suspicious for low level monotypic serum free lambda light chains (based only on the free light ratio). Immunopathology results showed no overt evidence of monoclonal bands. Protein electrophoresis pattern showed decreased albumin fraction and increased alpha, beta, and gamma fractions. C1 inhibitor was slightly elevated while serum tryptase was within normal values. Patient was managed as a case of idiopathic systemic capillary leak syndrome or Clarkson’s disease. Aside from judicious fluid resuscitation and vasopressors assist Methylprednisolone pulse therapy (MPPT) and intravenous immunoglobulin (IVIg) were administered. Patient was discharged with tapering doses of oral steroids and scheduled monthly IV immunoglobulin infusion. Conclusion: Cardiac tamponade, an uncommon yet severe and life-threatening complication of systemic capillary leak syndrome (SCLS) or Clarkson’s disease, requires immediate intervention. While the exact cause remains unclear, research suggests that during acute SCLS episodes and hyperinflammatory states, fluid accumulation in the pericardial space can impede ventricular filling, leading to hemodynamic compromise.

ISOLATION AND CHARACTERIZATION OF MAJOR SEED STORAGE PROTEINS: II. APIACEAE FAMILY FOUND IN SINDH, PAKISTAN

&#x0D; &#x0D; &#x0D; Seeds of 6 medicinally important plants they belong to family Apiaceae growing in Sindh province of Pakistan, including Anethum graveolens - dill, Apium graveolens - celery, Coriandrum sativum - coriander, Cuminum cyminum - cumin, Foeniculum vulgare -fennel, Trachyspermum ammi L - Carom, the deduction of four main seed storage proteins i.e. albumin, globulin, prolamin and glutelin. All plant species found and cultivated in Sindh, Pakistan. Proteins were seed flour was extracted by sequential steps of extractions including delipidation (removal of oil), water (albumin), 5.0 M NaCl (globulin), 70% ethonal (prolamin), and 0.2 M Na3PO4 buffer, pH8.0 (glutelin). Quantitative estimation was performed Dye binding technique of Bradford used for quantifiable estimation and found huge differences in terms of their concentrations and overall production (Table-1). Among all seed plants the albumin fraction was observed high in family Apiaceae where, C. cyminum (85.01%) and T. ammi L (47.12%) containing the highest, A. graveolans, F. vulgare, C. sativam L (37.66%, 36.88%, 30.14%, respectively) contains the medium while, the lowest concentration was observed in A. graveolans L. (29.11%). Globulin with the second dominant protein fraction may also vary from 3.52% in C. cyminum to 50% in C. sativam L. The meaningful increase in prolamin was observed in T. ammi L (33.08%), A. graveolans L (28.11%), A. graveolans, F. vulgare (21.34%) while, the lowest of around (9.6%) in C. cyminum, C. sativam L seeds. On the other hand, a consistent pattern of 5 to 20% of glutelin concentration was detected among every plant seeds (with exception of A. graveolans L having 23%). To the help of our research information, this study for the first time reported the comparative seed storage proteins profile of the family Apiacea and its possible medicinal and biotechnological application.&#x0D; &#x0D; &#x0D;

Physico-chemodiversity variation between the most common calcareous red seaweed, Eastern Harbor, Alexandria, Egypt

The present study sought to detect the difference in physicochemical properties and the proximate compositions of common calcareous red seaweeds "Corallina officinalis, Jania rubens, and Amphiroa rigida". J. rubens was recorded highly water (WHC), and oil holding capacity (OHC) (1.619 ± 0.08 g g−1 DW, 3.1 ± 0.50 g g−1 DW, respectively) than those other species relating to the hydrophilic nature of polysaccharides, whereas swelling water capacity (SWC) was higher in A. rigida (5.25 ± 0.38 mL g−1 DW). A higher value of carbohydrates (49.88 ± 2.56% DW) was observed in J. rubens, Contrariwise, protein (36.99 ± 1.36% DW) and lipid contents (5.85 ± 0.49% DW) were higher in C. officinalis. Albumin and protamine protein fractions were higher in J. rubens specimens (45.11 ± 2.29 mg g−1 & 0.0014 ± 0.0007 mg g−1 DW, respectively) than other species. While globulin and glutilin (31.70 ± 1.90 mg g−1 DW & 41.93 ± 2.20 mg g−1 DW, respectively) were high in A. rigida. Contrariwise, insoluble protein fraction was high in C. officinalis (9.50 ± 0.50 mg g−1 DW). J. rubens specimens were recorded maximum values of the photosynthetic pigments. The different surface types and elemental analysis of three species were examined by Scanning electron microscopy (SEM) and Energy dispersive X-ray spectroscopy (EDX). From fourier transform infrared (FTIR) spectroscopy the S–S stretching peak of disulfides group at 462 cm−1 was the fingerprint of J. rubens. From GC-MS data A.rigida possesses 16 bioactive components with biological properties. As a result, J. rubens and A. rigida could be employed as an ingredient in functional foods and drug manufacture.