The plant was selected as the source of the nanoscale study because it possesses more chemical compounds than the other plants,ZnO nanoparticles ( ZnONPs) were prepared from the plant Artemisia herba alba extract and from the flavonoid compound. The samples were diagnosed by Atomic Force Microsco (AFM) and Fourier transform infrared (FT-IR) spectroscopy , and effect ZnONPs the synthesis from Artemisia herba alba extract , from the flavonoid compound , Artemisia herba alba and flavonoid on the criteria for oxidative stress (Malonaldehyde MDA, Lipid peroxidation level LPO) and antioxidants (GSH glutation) were determine effective time and effective dose. The results showed that MDA and LPO showed a significant decrease compared with the control group, while the period of 32 days showed a significant decrease compared to 62 days. The analysis of GSH found that the coefficients of the study showed a significant increase compared with the control, while the period of 32 days showed a significant decrease compared with 62 days.