The impact of CO 2 leakage on solubility and distribution of trace metals in seawater and sediment has been studied in lab scale chambers. Seven metals (Al, Cr, Ni, Pb, Cd, Cu, and Zn) were investigated in membrane-filtered seawater samples, and DGT samplers were deployed in water and sediment during the experiment. During the first phase (16 days), “dissolved” (< 0.2 µm) concentrations of all elements increased substantially in the water. The increase in dissolved fractions of Al, Cr, Ni, Cu, Zn, Cd and Pb in the CO 2 seepage chamber was respectively 5.1, 3.8, 4.5, 3.2, 1.4, 2.3 and 1.3 times higher than the dissolved concentrations of these metals in the control. During the second phase of the experiment (10 days) with the same sediment but replenished seawater, the dissolved fractions of Al, Cr, Cd, and Zn were partly removed from the water column in the CO 2 chamber. DNi and DCu still increased but at reduced rates, while DPb increased faster than that was observed during the first phase. DGT-labile fractions (Me DGT) of all metals increased substantially during the first phase of CO 2 seepage. DGT-labile fractions of Al, Cr, Ni, Cu, Zn, Cd and Pb were respectively 7.9, 2.0, 3.6, 1.7, 2.1, 1.9 and 2.3 times higher in the CO 2 chamber than that of in the control chamber. Al DGT, Cr DGT, Ni DGT, and Pb DGT continued to increase during the second phase of the experiment. There was no change in Cd DGT during the second phase, while Cu DGT and Zn DGT decreased by 30% and 25%, respectively in the CO 2 chamber. In the sediment pore water, DGT labile fractions of all the seven elements increased substantially in the CO 2 chamber. Our results show that CO 2 leakage affected the solubility, particle reactivity and transformation rates of the studied metals in sediment and at the sediment–water interface. The metal species released due to CO 2 acidification may have sufficiently long residence time in the seawater to affect bioavailability and toxicity of the metals to biota.