Ecologists aim at disentangling how species vary in abundance through spatial and temporal scales, using a range of sampling techniques. Here, we investigated the circadian rhythm of seagrass-associated decapod crustaceans through three sampling techniques. Specifically, we compared the abundance, biomass and structure of seagrass-associated decapod assemblages between the day and night using a hand net, an airlift pump and baited traps. At night, the hand-net consistently collected a larger total abundance and biomass of decapods, what resulted in significant diel differences, which were detected for the total biomass, but not for the total abundance, when decapods were sampled through an airlift pump. Traps, however, collected a larger total abundance, but not total biomass, of decapods during the night. In summary, our perception of faunal diel rhythms is notably influence by the way organisms are sampled.