This manuscript reports the study of different types of interactions of chlorpheniramine maleate (CPAM) in aqueous solution using volumetric and viscometric parameters. Viscosity measurements were also used to calculate thermodynamic parameter for viscous flow. The critical micelle concentration (CMC) of drug was determined at four different temperatures using conductivity, surface tension and refractive index measurements. The data was also used to calculate different important parameters like surface excess concentration, area per molecule, Gibbs energy of adsorption, Gibbs energy of micellization, enthalpy of micellization, entropy of micellization, dissociation constant etc. These parameters are useful to understand the processes of micellization and adsorption of drug at solution-air interface. An idea of drug-biomembrane interaction in vitro was drawn with the help of drug-surfactant interaction study. Two amino acids i.e. glycine and L-tryptophan were selected to change the CMC of drug so that its decrease in transport rate through membrane due to micelle formation may be avoided.The results showed the spontaneous nature of adsorption of drug at solution-air interface and its micellization. CPAM showed strong interaction with anionic surfactant (SDS) by adsorption of its molecules between ionic heads of surfactant micelles which decreased CMC of surfactant. The presence of amino acids in solution caused the CMC of the drug to increase.