Air Samples Research Articles

Enhancing Surgical Safety: Microbiological Air Control in Operating Theatres at University Medical Centre Maribor.

the aim of the study was to assess microbiological air quality in operating theatres by determining the level of microbiological contamination of the air and critical surfaces using the passive air sampling method and compliance of the operating theatre staff with infection control measures. The prospective study was conducted in the surgical block of the University Medical Centre Maribor. For two months continuously, ten operating theatres were assessed for microbial contamination of air and surfaces during quiet and active times of the day. A passive air sampling method with Petri dishes on an agar specially adapted for this purpose (plate count agar) was used. In addition, ten surgical procedures were observed to assess staff compliance with recommended practises. Air samples met microbiological standards in all operating theatres. In both sampling sessions of the day (quiet and active periods), microbial contamination of the air was always within the limit of 10 CFU/m3. The average number of bacterial colonies was zero to two during quiet phases and one to four during active phases. Approximately 60% of the isolates from the operating theatres belonged mainly to the genus Staphylococcus: S. epidermidis (36% of the isolates), S. hominis (17.5%) and S. haemolyticus (5.5%). The rest were identified as Streptococcus anginosus (23%) and Bacillus sp. (18%). Pathogenic bacteria and moulds were not present. In regard to staff compliance with good surgical practise, the former varied by behaviour and function, with non-compliance in pre-operative skin preparation and operating theatre congestion being notable. The cleanliness of the environment was satisfactory. Microbiological air control is extremely important for the safety and success of both surgical and postoperative practises. In spite of good results obtained in the study, further improvements in surgical staff compliance with good surgical practise are essential to reduce surgical site infections.

Assessing exposure to airborne fungi and bacteria in the horse-riders environment: A pilot study

Among airborne biological contaminants, several pathogenic and/or allergenic fungi and bacteria can affect the health of horses and humans. Exposure to primary biological aerosol particles (PBAP) in the equine environment remains poorly understood, although environmental factors play a major role in the development of respiratory diseases and impaired performance in horses. This pilot study was conducted during a training course for young racers. Ambient and personal air samples provided original data on the microbial composition of PBAP in the riders’ immediate environment (stable, outdoor, riding arena), using culture-based and metagenomic approaches. The culture-based approach showed average levels of fungi and bacteria of 2.3 × 104 and 8.3 × 105 CFU/m3 respectively during training. Seventy-one and 50 fungal and bacterial species were respectively identified, while the metagenomic approach identified 211 and 285, respectively. It should be noted, however, that two-thirds of the bacterial and fungal genera were found by both approaches. Among these species, various pathogenic and/or allergenic fungi (Alternaria spp., Aspergillus spp., Cladosporium spp. and Lichtheimia ramosa) and bacteria (Aeromonas spp., Bacillus spp., Pseudomonas spp. and Staphylococcus spp.) were highlighted. This study underlines the value of the combined use of culture-based and metagenomic approaches to provide an overview of the distribution of different fungal and bacterial phyla, genera and species in the equine environment. Statistical analysis also suggests interactions between microbiological contaminants (bacteria and mold) and climatic parameters (rain, wind, temperature).

A novel approach to assess the health risk of aryl hydrocarbon receptor-bound contaminants via inhalation exposure using CYP1A1 expression as a biomarker

Polycyclic aromatic hydrocarbons (PAHs) and dioxins are potential causes of multiple diseases by activating the aryl hydrocarbon receptor (AhR) pathway. Health risk assessment of chemicals primarily relies on the relative potency factor (RPF), although its accuracy may be limited when solely using EC50 values. The induction of cytochrome P4501A1 (CYP1A1) serves as a biomarker for AhR activation and is an integrator of dioxin-like toxicity. Here, we present a method for evaluating the risks associated with AhR activation using mathematical models of dose-CYP1A1 induction. The dose-effect curves for certain PAHs and dioxins, including Ant, BghiP, 1,2,3,4,7,8-HxCDD, and others, exhibited a non-classical S-shaped form. The toxic equivalent factor (TEF) profiles revealed a broad range of toxic equivalent factor values. The TEFs for PAHs ranged from approximately 0.01 to 6, with higher values being observed when the concentration was less than 10−10 M, with the exceptions of Ace, Phe, and BghiP. Most congeners of dioxins got the lowest TEF value at around 10−10 M, ranging from 0.04 to 1.00. The binding affinity of AhR to ligands did not display a strong correlation with the EC50 of CYP1A1 expression, suggesting that the AhR-mediated effects of PAHs and dioxins are not fixed but instead fluctuate with the dose. Air samples acquired from a parking area were used to compare the proficiency of RPF and our current approach. In the current method, naphthalene and chrysene were the primary contributors of PAHs to AhR-mediated risks in parking lots air samples, respectively. However, the contributions of naphthalene and chrysene could be disregarded in the RPF approach.

Technological Maturity of Aircraft-Based Methane Sensing for Greenhouse Gas Mitigation.

Methane is a major contributor to anthropogenic greenhouse gas emissions. Identifying large sources of methane, particularly from the oil and gas sectors, will be essential for mitigating climate change. Aircraft-based methane sensing platforms can rapidly detect and quantify methane point-source emissions across large geographic regions, and play an increasingly important role in industrial methane management and greenhouse gas inventory. We independently evaluate the performance of five major methane-sensing aircraft platforms: Carbon Mapper, GHGSat-AV, Insight M, MethaneAIR, and Scientific Aviation. Over a 6 week period, we released metered gas for over 700 single-blind measurements across all five platforms to evaluate their ability to detect and quantify emissions that range from 1 to over 1,500 kg(CH4)/h. Aircraft consistently quantified releases above 10 kg(CH4)/h, and GHGSat-AV and Insight M detected emissions below 5 kg(CH4)/h. Fully blinded quantification estimates for platforms using downward-facing imaging spectrometers have parity slopes ranging from 0.76 to 1.13, with R2 values of 0.61 to 0.93; the platform using continuous air sampling has a parity slope of 0.5 (R2 = 0.93). Results demonstrate that aircraft-based methane sensing has matured since previous studies and is ready for an increasingly important role in environmental policy and regulation.

The Ins and Outs of Per- and Polyfluoroalkyl Substances in the Great Lakes: The Role of Atmospheric Deposition.

As part of the Integrated Atmospheric Deposition Network, precipitation (n = 207) and air (n = 60) from five sites and water samples (n = 87) from all five Great Lakes were collected in 2021-2023 and analyzed for 41 per- and polyfluoroalkyl substances (PFAS). These measurements were combined with other available data to estimate the mass budget for four representative compounds, PFBA, PFBS, PFOS, and PFOA for the basin. The median Σ41PFAS concentrations in precipitation across the five sites ranged between 2.4 and 4.5 ng/L. The median Σ41PFAS concentration in lake water was highest in Lake Ontario (11 ng/L) and lowest in Lake Superior (1.3 ng/L). The median Σ41PFAS concentration in air samples was highest in Cleveland at 410 pg/m3 and lowest at Sleeping Bear Dunes at 146 pg/m3. The net mass transfer flows were generally negative for Lakes Superior, Michigan, and Huron and positive for Lakes Erie and Ontario, indicating that the three most northern lakes are accumulating PFAS and the other two are eliminating PFAS. Atmospheric deposition is an important source of PFAS, particularly for Lake Superior.

Laser-induced electro-response of oil shale: Principles and applications

This study introduces a laser-induced electro-response (LIER) method to address the challenges arising from the harsh detection environment and energy consumption in the conversion engineering of oil shale. LIER system with pulsed laser and continuous-wave laser was used to investigate the feasibility of oil yield detection and real-time pyrolysis characterization, respectively. The equivalent circuit model for oil shale measurement under the pulse laser irradiation was established, considering the laser-induced plasma and photoelectric effect. While the laser-induced thermal effect is the main cause of the LIER response of oil shale under continuous-wave laser irradiation. Due to the complex interactions between laser and oil shale, the LIER of oil shale is affected by many factors, such as external electric field, laser source type, laser wavelength, and laser energy or power. The external electric field can improve the responsivity of LIER. The LIER signal of oil shale strongly positively correlates with oil yield. Hence, the LIER evaluated oil yield more quickly without pyrolysis than traditional methods, which is helpful for the exploration for oil shale. In addition, the multi-stage LIER responses were monitored in real time during the combustion and pyrolysis processes of oil shale samples in air and N2 atmosphere, respectively, realizing an evaluation of the organic and mineral decomposition, which provides a supplementary approach to conventional chemical techniques. These results show that LIER has great significance for the precise evaluation and effective development of oil shale reservoirs, which will drive the advances in the exploration for oil shale.

Assessment of Occupational Exposure to Inhalable Aerosols in an Instant Powdered Food Manufacturing Plant in Norway

BackgroundIn the food manufacturing industry, exposure to inhalable aerosols contributes to respiratory illnesses such as occupational asthma and rhinitis. However, there is a lack of comprehensive exposure assessment studies. This study evaluated occupational exposure to inhalable aerosols in an instant powdered food manufacturing plant during work operations involving dried food and powders. MethodsIn total, 50 workers from an instant powdered food manufacturing plant were recruited. Personal inhalable aerosol exposure measurements were taken for both full-shift and task-based activities. The concentrations of inhalable aerosols were analyzed to identify any variation within and across departments, as well as between seasons, handedness, and sex. ResultsIn total, 134 personal air samples were collected, and the particulate mass was determined gravimetrically. The concentrations of inhalable aerosols ranged from 0.1 to 27 mg/m3 for full-shift exposure measurements and 3.1 to 73 mg/m3 for task-based measurements. Statistically significant differences in mean aerosol concentrations were found across departments (A:B p < 0.001, A:C p < 0.05, B:C p < 0.001) and between seasons (p < 0.001). ConclusionThis study revealed high exposure to inhalable aerosols among workers, particularly those involved in manual weighing, mixing, and adding powders. The significant differences between departments highlight the specific activities contributing to increased inhalable aerosol concentrations. Seasonal variations were also evident, with autumn showing higher concentrations of inhalable aerosols in all departments compared with summer. These findings emphasize the importance of understanding the distribution of aerosol concentrations across different work tasks and departments, particularly during different seasons.

Comprehensive study on potato drying in convective air dryer: experimental observations, mathematical modeling, and model validation

We studied the drying kinetics of sliced potatoes (Solanum tuberosum) using a tray dryer. An experiment was conducted on how the drying rate was impacted by air temperature, sample thickness, and air velocity. The study was conducted under varying temperature ranges (50°C, 60°C, and 70°C), air velocities (0.6 ms−1, 0.7 m s−1, and 0.8 m s−1), and potato slice thickness (2 mm, 4 mm, and 6 mm). The findings revealed that a higher air temperature was correlated with an increase in the drying rate, while thicker layers demonstrated a decrease in the drying rate. We further evaluated five mathematical models, with the Page Model emerging as the most suitable based on the lowest residual standard error (RSE) and Akaike's Information Criteria (AIC), which are 0.06421 and -1123.947, respectively. After plotting the predicted moisture ratio against the experimental moisture ratio, we observed an exceptional predictive accuracy (R2 > 0.99) across various conditions. These findings underscore the critical importance of precise parameter control for optimizing potato drying processes in industrial settings, providing valuable insights for enhanced efficiency and resource utilization. Further exploration into real-time optimization and scalability can extend the practical applications of this study.

Occurrence and seasonal variations of organophosphate flame retardants in air and dust from college microenvironments at Qingdao, China: Implications for student's exposure and risk assessment

Organophosphate flame retardants (OPFRs) are widely used as alternatives to brominated flame retardants in a variety of consumer products and their consumption has continuously increased in recent years. However, their concentrations and human exposures in indoor microenvironments, particularly in a university environment, have received limited attention. In this study, the concentrations and seasonal variations of 15 OPFRs were assessed in typical microenvironments of two universities, including dormitories, offices, public microenvironments (PMEs: classroom, dining hall, gymnasium and library), and laboratories on the northern coast of China.Analysis of the OPFRs in both air and dust samples indicated widespread distribution in college campuses. The average concentration of ∑15OPFRs in the winter (12,774.4 ng/g and 5.3 ng/m3 for dust and air, respectively) was higher than in the summer (2460.4 ng/g and 4.6 ng/m3 for dust and air, respectively). The dust and air samples collected from PMEs and laboratories exhibited higher concentrations of OPFRs, followed by offices and dormitories. An equilibrium was reached between dust and air in all collected microenvironments. The daily intakes of OPFRs were significantly lower than the reference dose. Dust ingestion was the primary intake pathway in the winter, while inhalation and dust ingestion were the main intake pathways in the summer. The non-carcinogenic hazard quotients fell within the range of 10−7–10−3 in both the summer and winter, which are below the theoretical risk threshold. For the carcinogenic risk, the LCR values ranged from 10−10 to 10−8, indicating no elevated carcinogenic risk due to TnBP, TCEP, and TDCP in indoor dust and air.

Regulatory compliance of PCDD/F emissions by a municipal solid waste incinerator. A case study in Sant Adrià de Besòs, Catalonia, Spain

Despite incineration is an important emission source of toxic pollutants, such as heavy metals and polychlorinated dibenzo-p-dioxins and dibenzofurans (PCDD/Fs), it is still one of the most widely used methods for the management of municipal solid waste. The current paper summarizes the results of a 20-year follow-up study of the emissions of PCDD/Fs by a municipal solid waste incinerator (MSWI) in Sant Adrià de Besòs (Catalonia, Spain). Samples of ambient air, soils and herbage were periodically collected near the facility and the content of PCDD/Fs was analyzed. In the last (2017) survey, mean levels in soil were 3.60 ng WHO-TEQ/kg (range: 0.40–10.6), being considerably higher than the mean concentrations of PCDD/Fs in soil samples collected near other MSWIs in Catalonia. Moreover, air PCDD/F concentrations were even higher than those found in a previous (2014) survey, as they increased from 0.026 to 0.044 pg WHO-TEQ/m3. Ultimately, the PCDD/F exposure would be associated to a cancer risk (2.5 × 10−6) for the population living in the surrounding area. Globally, this information indicates that the MSWI of Sant Adrià de Besòs could have had a negative impact on the environment and potentially on public health, being an example of a possible inappropriate management for years. The application of Best Available Techniques to minimize the emission of PCDD/Fs and other chemicals is critical.

What potential do mosses have as biomonitors of POPs? A comparative study of hexachlorocyclohexane sorption

Persistent organic pollutants (POPs) pose a significant global threat to human health and the environment, and require continuous monitoring due to their ability to migrate long distances. Active biomonitoring using cloned mosses is an inexpensive but underexplored method to assess POPs, mainly due to the poor understanding of the loading mechanisms of these pollutants in mosses. In this work, Fontinalis antipyretica (aquatic moss) and Sphagnum palustre (terrestrial moss) were evaluated as potential biomonitors of hexachlorocyclohexanes (HCHs: α-, β-, γ-, δ-HCH), crucial POPs. Moss clones, grown in photobioreactors and subsequently oven-dried, were used. Their lipid composition and distribution were characterized through molecular and histochemical studies. Adsorption experiments were carried out in the aqueous phase using the repeated additions method and in the gas phase using an active air sampling technique based on solid-phase extraction, a pioneering approach in moss research. F. antipyretica exhibited greater lipid content in the walls of most cells and higher adsorption capacity for all HCH isomers in both gaseous and liquid environments. These findings highlight the need for further investigation of POP loading mechanisms in mosses and open the door to explore other species based on their lipid content.

Bioaerosol Sampling Devices and Pretreatment for Bacterial Characterization: Theoretical Differences and a Field Experience in a Wastewater Treatment Plant.

Studies on bioaerosol bacterial biodiversity have relevance in both ecological and health contexts, and molecular methods, such as 16S rRNA gene-based barcoded sequencing, provide efficient tools for the analysis of airborne bacterial communities. Standardized methods for sampling and analysis of bioaerosol DNA are lacking, thus hampering the comparison of results from studies implementing different devices and procedures. Three samplers that use gelatin filtration, swirling aerosol collection, and condensation growth tubes for collecting bioaerosol at an aeration tank of a wastewater treatment plant in Trieste (Italy) were used to determine the bacterial biodiversity. Wastewater samples were collected directly from the untreated sewage to obtain a true representation of the microbiological community present in the plant. Different samplers and collection media provide an indication of the different grades of biodiversity, with condensation growth tubes and DNA/RNA shieldTM capturing the richer bacterial genera. Overall, in terms of relative abundance, the air samples have a lower number of bacterial genera (64 OTUs) than the wastewater ones (75 OTUs). Using the metabarcoding approach to aerosol samples, we provide the first preliminary step toward the understanding of a significant diversity between different air sampling systems, enabling the scientific community to orient research towards the most informative sampling strategy.

A fieldable process for sensitive detection of airborne viruses via electrophoresis-based RNA enrichment

Sensitive on-site virus detection is a requirement for timely action against the spread of airborne infection since ultra-low in-air viral concentrations can readily infect individuals when inhaled. Here, we consider a fieldable biosensing process that incorporates a fast RNA enrichment step in order to concentrate viral RNA in a small volume prior to RT-qPCR. The enrichment approach uses electrophoresis in an RT-qPCR-compatible buffer, and allows for concentration of RNA by nearly 5-fold within only 10 min. In order to place this performance into context, we analyzed the minimum detectable concentration of a low-cost, fieldable, biosensing process that uses electrostatic precipitation for air sampling, heating for viral RNA extraction, and then RNA enrichment, followed by RT-qPCR. With enrichment, we estimated an in-air concentration of 5,654 genome copies (gc)/m3 with a 100% detection rate and an in-air concentration of 4,221 gc/m3 with a 78.6% detection rate. Given that the concentrations of common viruses, such as influenza and SARS-CoV-2, in several indoor spaces are between 5,800 and 37,000 gc/m3, we conclude that enrichment allows a detection that is sufficiently sensitive for practical applications.

A Loop-Mediated Isothermal Amplification (LAMP) Assay for the Detection of Bremia lactucae in the Field.

A real-time loop-mediated isothermal amplification (LAMP) assay for the detection of Bremia lactucae, the causal pathogen of lettuce downy mildew, was developed and validated to aid in-field detection of airborne inoculum. Assay specificity was confirmed against a range of other pathogenic oomycete and fungal spp., and sensitivity of the assay for the detection of DNA extracted from sporangia was evaluated. The B. lactucae LAMP assay reliably detected DNA equivalent to 1 spore/reaction (16.7 pg DNA/reaction). Following extraction of DNA from Rotorod air samplers, to which sporangial suspensions were added, the assay reliably detected 25 sporangia/Rotorod. Detection of airborne inoculum of B. lactucae collected through the season from air samplers deployed in-field in plots infected with B. lactucae and in commercial lettuce fields in Scotland over two growing seasons was assessed. The method can be deployed on samples collected from commercial lettuce production to inform disease management strategies and limit the use of unnecessary prophylactic pesticide applications.

Indoor and outdoor NO2 and SO2 levels in 13 randomly selected preschools from 7 districts in Mpumalanga Province, South Africa

AbstractBackgroundAir pollution is a serious worldwide issue, where both outdoor and indoor air quality have a substantial impact on the health of children. Children are more vulnerable to the effects of air pollution due to their developing respiratory systems and higher respiration rates than adults. These children dedicate a substantial amount of time to the preschool setting. The study sought to evaluate the concentration of NO2 and SO2 in both outdoor and indoor environments of preschool facilities.MethodsConcurrently, the levels of NO2 and SO2 were measured indoors and outdoors at 13 randomly selected preschools in 7 districts in Mpumalanga Province, South Africa. Radiello passive air samplers were employed to collect gaseous samples.ResultsThe mean levels of NO2 indoors and outdoors were within the recommended criteria outlined by the World Health Organization (WHO), specifically target levels 1–3. Additionally, these values were also below the air quality guides outlined by South Africa, both for yearly and 1‐h mean measurements. Nevertheless, both levels remained above the suitable threshold of 10 µg m−3 outlined by the WHO air quality guidelines.ConclusionsIt is important to implement proactive measures such as enhancing airflow systems, employing air filters and reducing potential sources of air pollution in preschools, to aid in reducing exposure to indoor and outdoor NO2 and SO2.

How safe are 3D-printed skull models for neurosurgical simulation? Measurement of airborne particles and VOCs while burr hole drilling

PurposeThree-dimensional (3D)printed skulls for neurosurgical training are increasingly being used due to the widespread access to 3D printing technology, their low cost and accuracy, as well as limitations and ethical concerns associated with using human cadavers. However, little is known about the risks of airborne particles or volatile organic compounds (VOCs) released while drilling into 3D-printed plastic models. The aim of this study is to assess the level of exposure to airborne contaminants while burr hole drilling.Design/methodology/approach3D-printed skull samples were produced using three different materials (polyethylene terephthalate glycol [PETG], white resin and BoneSTN) across three different 3D print processes (fused filament fabrication, stereolithography [SLA] and material jetting). A neurosurgeon performed extended burr hole drilling for 10 min on each sample. Spot measurements of particulate matter (PM2.5 and PM10) were recorded, and air samples were analysed for approximately 90 VOCs.FindingsThe particulate matter for PETG was found to be below the threshold value for respirable particles. However, the particulate matter for white resin and BoneSTN was found to be above the threshold value at PM10, which could be harmful for long periods of exposure without personal protective equipment (PPE). The VOC measurements for all materials were found to be below safety thresholds, and therefore not harmful.Originality/valueTo the best of the authors’ knowledge, this is the first study to evaluate the safety of 3D-printed materials for burr hole surgical drilling. It recommends PETG as a safe material requiring minimal respiratory control measures, whereas resin-based materials will require safety controls to deal with airborne particles.

Tracking bioaerosol exposure among municipal solid waste workers using hematological and inflammatory biomarkers

High levels of bioaerosols may exist in the air of municipal solid waste (MSW) management facilities, constituting a significant occupational hazard for workers. In this study, we investigated the potential association between exposure to bioaerosols and inflammatory biomarkers among municipal solid waste workers (MSWWs) at both the landfill site and the municipal solid waste transfer station (MSWTS), in comparison to a control group without exposure. Air sampling was conducted at six points around the landfill, two points at the MSWTS, and one point in a public park (as a control area) during the spring and summer of 2019. The results of our study revealed that airborne pathogens were highly prevalent at the sampling points, especially in the active zone and leachate collection pond. Aspergillus species were the predominant fungal species detected in this study, with the highest occurrence observed for Aspergillus flavus (83.3%), Aspergillus niger, and Aspergillus fumigatus (75.0%). Furthermore, Staphylococcus species accounted for over 75% of the total bacterial bioaerosols detected across all study areas. The blood test results of workers revealed a significant increase in platelets (PLT), immunoglobulin G (IgG), white blood cells (WBC), neutrophils, basophils, and high-sensitivity C-reactive protein (hs-CRP) compared to the control group. Conversely, platelet distribution width (PDW), mean platelet volume (MPV), and platelet-large cell ratio (P-LCR) in the exposed subjects exhibited a decreasing trend compared to the control group. These findings suggest a potential association between exposure to bioaerosols, particularly in the vicinity of open dumpsites, and elevated levels of hematologic and inflammatory markers in circulation. Furthermore, the influence of smoking status and confounding factors appears to be significant in both the control and exposure groups.

Flame Retardant Exposure in Vehicles Is Influenced by Use in Seat Foam and Temperature.

Flame retardants (FRs) are added to vehicles to meet flammability standards, such as US Federal Motor Vehicle Safety Standard FMVSS 302. However, an understanding of which FRs are being used, sources in the vehicle, and implications for human exposure is lacking. US participants (n = 101) owning a vehicle of model year 2015 or newer hung a silicone passive sampler on their rearview mirror for 7 days. Fifty-one of 101 participants collected a foam sample from a vehicle seat. Organophosphate esters (OPEs) were the most frequently detected FR class in the passive samplers. Among these, tris(1-chloro-isopropyl) phosphate (TCIPP) had a 99% detection frequency and was measured at levels ranging from 0.2 to 11,600 ng/g of sampler. TCIPP was also the dominant FR detected in the vehicle seat foam. Sampler FR concentrations were significantly correlated with average ambient temperature and were 2-5 times higher in the summer compared to winter. The presence of TCIPP in foam resulted in ∼4 times higher median air sampler concentrations in winter and ∼9 times higher in summer. These results suggest that FRs used in vehicle interiors, such as in seat foam, are a source of OPE exposure, which is increased in warmer temperatures.

Review on Sampling Methods and Health Impacts of Fine (PM2.5, ≤2.5 µm) and Ultrafine (UFP, PM0.1, ≤0.1 µm) Particles

Airborne particulate matter (PM) is of great concern in the modern-day atmosphere owing to its association with a variety of health impacts, such as respiratory and cardiovascular diseases. Of the various size fractions of PM, it is the finer fractions that are most harmful to health, in particular ultrafine particles (PM0.1; UFPs), with an aerodynamic diameter ≤ 100 nm. The smaller size fractions, of ≤2.5 µm (PM2.5; fine particles) and ≤0.1 µm (PM0.1; ultrafine particles), have been shown to have numerous linkages to negative health effects; however, their collection/sampling remains challenging. This review paper employed a comprehensive literature review methodology; 200 studies were evaluated based on the rigor of their methodologies, including the validity of experimental designs, data collection methods, and statistical analyses. Studies with robust methodologies were prioritised for inclusion. This review paper critically assesses the health risks associated with fine and ultrafine particles, highlighting vehicular emissions as the most significant source of particulate-related health effects. While coal combustion, diesel exhaust, household wood combustors’ emissions, and Earth’s crust dust also pose health risks, evidence suggests that exposure to particulates from vehicular emissions has the greatest impact on human health due to their widespread distribution and contribution to air pollution-related diseases. This article comprehensively examines current sampling technologies, specifically focusing on the collection and sampling of ultrafine particles (UFP) from ambient air to facilitate toxicological and physiochemical characterisation efforts. This article discusses diverse approaches to collect fine and ultrafine particulates, along with experimental endeavours to assess ultrafine particle concentrations across various microenvironments. Following meticulous evaluation of sampling techniques, high-volume air samplers such as the Chem Vol Model 2400 High Volume Cascade Impactor and low-volume samplers like the Personal Cascade Impactor Sampler (PCIS) emerge as effective methods. These techniques offer advantages in particle size fractionation, collection efficiency, and adaptability to different sampling environments, positioning them as valuable tools for precise characterisation of particulate matter in air quality research and environmental monitoring.

Microplastics in remote region of the world: Insights from the glacier of Geladandong, China

Although microplastics have been detected in different remote areas, people are still short of knowledge for environmental background of microplastics in remote area. In this work, we collected air, snow, river and lake water samples from Geladandong region (the origin of the Yangtze River) of the Tibetan Plateau, along with microscopy and Micro-FTIR analyses. Microplastics abundance (0.13–19.8 items/L) was the low as compared with other remote areas worldwide, and the morphology of fiber and film, material of PP and PET, and size of <300 μm were the main microplastics features. Due to natural aging during the transport processes, microplastics were degrading to smaller particle in Geladandong. Air mass trajectory analysis shows microplastics in Geladandong is transferred by repeated atmospheric transport from no human activity place. All these results suggested that Geladandong is an ideal research area for environmental background value and characteristic of microplastics. In conjunction with data from other remote regions, we established the background values for microplastics in remote areas. This work provides quantitative evidence for establishing environmental quality standards, and monitoring environmental changes.